Document
... BIOPHYSICS-BASED DESIGN Characterise sequences and structures of naturally occurring proteins in terms of their total similarity scores using different scoring matrices. This will produce a database of sequences with predicted and known structures with specific selectivity and affinity to different ...
... BIOPHYSICS-BASED DESIGN Characterise sequences and structures of naturally occurring proteins in terms of their total similarity scores using different scoring matrices. This will produce a database of sequences with predicted and known structures with specific selectivity and affinity to different ...
Supplementary Figures
... reading frames (orfs). Six Epsilon15 virion structural proteins were matched to orfs by mass spectrometric peptide mapping of trypsin digested phage proteins separated by SDS-PAGE, represented by the blue boxes in the genome map. Other genes were identified by homology to sequences of known function ...
... reading frames (orfs). Six Epsilon15 virion structural proteins were matched to orfs by mass spectrometric peptide mapping of trypsin digested phage proteins separated by SDS-PAGE, represented by the blue boxes in the genome map. Other genes were identified by homology to sequences of known function ...
BS3 Crosslinking
... previously (Grosshans et al., 2001, 2002; Conrad et al., 2008). BS3 is a membrane-impermeable agent, which selectively crosslinks cell-surface proteins to form high-molecular-mass aggregates. Because intracellular proteins are not modified, they retain normal molecular mass. This enables surface and ...
... previously (Grosshans et al., 2001, 2002; Conrad et al., 2008). BS3 is a membrane-impermeable agent, which selectively crosslinks cell-surface proteins to form high-molecular-mass aggregates. Because intracellular proteins are not modified, they retain normal molecular mass. This enables surface and ...
PowerPoint
... • However, φ and ψ of a given amino acid residue are limited due to steric hindrance • Only 10% of the {φ, ψ} combinations are generally observed for proteins • First noticed by G.N. Ramachandran ...
... • However, φ and ψ of a given amino acid residue are limited due to steric hindrance • Only 10% of the {φ, ψ} combinations are generally observed for proteins • First noticed by G.N. Ramachandran ...
Correlating heritable traits with specific gene products.
... surface protein also found in breast milk and has previously been identified as having a high similarity to the B7 family 7. -Three of the proteins corresponded to signal regulatory protein (SIRP)--1 and one to SIRP--1. Both of these are transmembrane proteins. SIRP--1 is an inhibitory receptor, ...
... surface protein also found in breast milk and has previously been identified as having a high similarity to the B7 family 7. -Three of the proteins corresponded to signal regulatory protein (SIRP)--1 and one to SIRP--1. Both of these are transmembrane proteins. SIRP--1 is an inhibitory receptor, ...
Receptor Tyrosine Kinases
... • NRTK’s associate with membrane receptors or multiprotein complexes which regulate their activity. • Activation involves both conformational changes and tyrosine phosphorylation of activation loop residues by heterologous kinases or autophosphorylation. • NRTK’s contain domains that mediate binding ...
... • NRTK’s associate with membrane receptors or multiprotein complexes which regulate their activity. • Activation involves both conformational changes and tyrosine phosphorylation of activation loop residues by heterologous kinases or autophosphorylation. • NRTK’s contain domains that mediate binding ...
biological process
... Result of an MeCP2 blastp search: A methyl-binding domain shared by several proteins ...
... Result of an MeCP2 blastp search: A methyl-binding domain shared by several proteins ...
Introduction to Macromolecular Structures
... The Universe of Protein Structures Protein motifs may be defined by their primary sequence or by the arrangement of secondary structure elements ...
... The Universe of Protein Structures Protein motifs may be defined by their primary sequence or by the arrangement of secondary structure elements ...
Structure determination by X
... The Universe of Protein Structures Protein motifs may be defined by their primary sequence or by the arrangement of secondary structure elements ...
... The Universe of Protein Structures Protein motifs may be defined by their primary sequence or by the arrangement of secondary structure elements ...
Translation
... • UGA – These codons are not recognized by tRNAs, but by proteins called release factors • Indeed, the 3-D structure of release factors mimics that of tRNAs ...
... • UGA – These codons are not recognized by tRNAs, but by proteins called release factors • Indeed, the 3-D structure of release factors mimics that of tRNAs ...
Brooker Chapter 13
... – In most species there are three stop or nonsense codons • UAG • UAA • UGA – These codons are not recognized by tRNAs, but by proteins called release factors • Indeed, the 3-D structure of release factors mimics that of tRNAs ...
... – In most species there are three stop or nonsense codons • UAG • UAA • UGA – These codons are not recognized by tRNAs, but by proteins called release factors • Indeed, the 3-D structure of release factors mimics that of tRNAs ...
File - Elko Science
... (water soluble) side chain are often found on the surface of the molecule while amino acids with nonpolar (water insoluble) side chain are buried in the interior. This means that the folded protein is soluble in water or aqueous solutions. + Disulfide Bonds: The polypeptide chains of some proteins a ...
... (water soluble) side chain are often found on the surface of the molecule while amino acids with nonpolar (water insoluble) side chain are buried in the interior. This means that the folded protein is soluble in water or aqueous solutions. + Disulfide Bonds: The polypeptide chains of some proteins a ...
Steps in a Western blot
... In order to make the proteins accessible to antibody detection, they are moved from within the gel onto a membrane made of nitrocellulose or PVDF. The membrane is placed on top of the gel, and a stack of tissue papers placed on top of that. The entire stack is placed in a buffer solution which moves ...
... In order to make the proteins accessible to antibody detection, they are moved from within the gel onto a membrane made of nitrocellulose or PVDF. The membrane is placed on top of the gel, and a stack of tissue papers placed on top of that. The entire stack is placed in a buffer solution which moves ...
general western blot troubleshooting tips
... Filter the secondary with a 0.2 µm filter to remove any aggregates. ...
... Filter the secondary with a 0.2 µm filter to remove any aggregates. ...
BIOMG 3310: Principles of Biochemistry
... Lecture Keywords: aliphatic hydroxyl amino acids, acidic amino acids, amide amino acids, basic amino acids, ubiquitin, proline, hydrophobic interaction, protein modification ...
... Lecture Keywords: aliphatic hydroxyl amino acids, acidic amino acids, amide amino acids, basic amino acids, ubiquitin, proline, hydrophobic interaction, protein modification ...
structures
... • Confidence predicted - based upon number of residues of given type at a given position in the alignment ...
... • Confidence predicted - based upon number of residues of given type at a given position in the alignment ...
21. Membranes
... cytoskeleton, but are still considered a part of the membrane. Similarly, proteins on the extracellular side are often held by the ECM. 2. Protein functions a. Transport i. Proteins provide the cell with the opportunity to transport material into and out of the cell, by active transport/facilitated ...
... cytoskeleton, but are still considered a part of the membrane. Similarly, proteins on the extracellular side are often held by the ECM. 2. Protein functions a. Transport i. Proteins provide the cell with the opportunity to transport material into and out of the cell, by active transport/facilitated ...
doc - Gogarten Lab
... 10. If two proteins are homologous, will their structures usually be alignable with the Swiss Protein DataBank Viewer? (1pt) a. Yes b. No c. Can’t tell- not enough information given Give a short justification of your reasoning: ...
... 10. If two proteins are homologous, will their structures usually be alignable with the Swiss Protein DataBank Viewer? (1pt) a. Yes b. No c. Can’t tell- not enough information given Give a short justification of your reasoning: ...
Essential Amino Acids
... Other protein comes from the recycling of enzymes and other proteins. Protein is synthesized in all tissues in the body; however, the liver and muscles are the most active. The body synthesizes about 300 grams of protein per day even though average intake is only 70 grams. ...
... Other protein comes from the recycling of enzymes and other proteins. Protein is synthesized in all tissues in the body; however, the liver and muscles are the most active. The body synthesizes about 300 grams of protein per day even though average intake is only 70 grams. ...
Ostlund Farrants
... Actively transcribed gene body – H3K36me3 Inactive enhancers and promoters – H3K27me3 and H3K9me3 ...
... Actively transcribed gene body – H3K36me3 Inactive enhancers and promoters – H3K27me3 and H3K9me3 ...
Histone Demethylation by A Family of JmjC Domain
... Figure S2. Schematic representation of the steps used in purifying the demethylase activity from HeLa cells. Numbers represent the salt concentrations (mM) at which the histone demethylase activity elutes from the column. Figure S3. Comparison of the JHDM1 family of proteins. a. Diagrammatic represe ...
... Figure S2. Schematic representation of the steps used in purifying the demethylase activity from HeLa cells. Numbers represent the salt concentrations (mM) at which the histone demethylase activity elutes from the column. Figure S3. Comparison of the JHDM1 family of proteins. a. Diagrammatic represe ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.