Whole-Brain Serial-Section Electron Microscopy In Larval Zebrafish
... variable. Consequently, ssEM of whole vertebrate neuronal circuits requires rapid computerbased technologies for acquiring, storing, and analysing many images from one animal. In many cases, anatomical data must be combined with other experiments on the same individual11-13 to define the relationshi ...
... variable. Consequently, ssEM of whole vertebrate neuronal circuits requires rapid computerbased technologies for acquiring, storing, and analysing many images from one animal. In many cases, anatomical data must be combined with other experiments on the same individual11-13 to define the relationshi ...
Dopamine Modulates the Activity of Sensory Hair Cells
... Correspondence should be addressed to Dr. Teresa Nicolson, Oregon Hearing Research Center and Vollum Institute, 3181 SW Sam Jackson Park Road, Oregon Health and Science University, Portland, OR 97239. E-mail: ...
... Correspondence should be addressed to Dr. Teresa Nicolson, Oregon Hearing Research Center and Vollum Institute, 3181 SW Sam Jackson Park Road, Oregon Health and Science University, Portland, OR 97239. E-mail: ...
Review Article Regeneration of Zebrafish CNS
... availability of genetic tools (only a few of them have recently been developed) proved these models relatively less appealing to the scientists. On the other hand, the zebrafish proves to be an excellent and popular model for a variety of reasons, for example, (a) very cost-effective maintenance and ...
... availability of genetic tools (only a few of them have recently been developed) proved these models relatively less appealing to the scientists. On the other hand, the zebrafish proves to be an excellent and popular model for a variety of reasons, for example, (a) very cost-effective maintenance and ...
Mutations affecting neural survival in the zebrafish Danio rerio
... largest category comprises 398 mutations that lead to massive and widespread CNS-specific cell death which becomes apparent only after somitogenesis and does not result in an abnormal brain morphology. Due to the abundance of these mutations, only a small subset of 16 mutations was maintained and wi ...
... largest category comprises 398 mutations that lead to massive and widespread CNS-specific cell death which becomes apparent only after somitogenesis and does not result in an abnormal brain morphology. Due to the abundance of these mutations, only a small subset of 16 mutations was maintained and wi ...
Zebrafish Get Connected: Investigating Neurotransmission Targets
... to a number of diseases including movement disorders, neuropsychiatric disorders, and depression (reviewed by [7–9]). The zebrafish (Danio rerio) research model is increasingly being used in neurotoxicity studies (as reviewed by [3,10–13]). Zebrafish share the common neurotransmitter pathways with m ...
... to a number of diseases including movement disorders, neuropsychiatric disorders, and depression (reviewed by [7–9]). The zebrafish (Danio rerio) research model is increasingly being used in neurotoxicity studies (as reviewed by [3,10–13]). Zebrafish share the common neurotransmitter pathways with m ...
Rapid Whole Brain Imaging Of Neural Activities In Freely
... bioRxiv preprint first posted online Apr. 28, 2017; doi: http://dx.doi.org/10.1101/131532. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ...
... bioRxiv preprint first posted online Apr. 28, 2017; doi: http://dx.doi.org/10.1101/131532. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ...
Expression of the zebrafish gene hlx
... from hlx-1 and the pax[b] gene. The latter is expressed within two transversal stripes located in the posterior midbrain region which fuse at the dorsal midline in 10 hpf embryos (Krauss et al., 1991b). At later stages, the posterior boundary of the pax[b] expression domain coincides with the furrow ...
... from hlx-1 and the pax[b] gene. The latter is expressed within two transversal stripes located in the posterior midbrain region which fuse at the dorsal midline in 10 hpf embryos (Krauss et al., 1991b). At later stages, the posterior boundary of the pax[b] expression domain coincides with the furrow ...
Limitations of Neural Map Topography for Decoding Spatial
... points in the brain. It is often assumed that this “topography” of wiring is essential for decoding sensory stimuli. However, here we show in the developing zebrafish that topographic decoding performs very poorly compared with methods that do not rely on topography. This suggests that, although wir ...
... points in the brain. It is often assumed that this “topography” of wiring is essential for decoding sensory stimuli. However, here we show in the developing zebrafish that topographic decoding performs very poorly compared with methods that do not rely on topography. This suggests that, although wir ...
Physiological study of larval fishes: challenges and opportunities
... present as general animal models for understanding vertebrate development. Larval fishes can serve as broadly applicable models because early stages of development are surprisingly similar across vertebrate taxa. These similarities can be exploited and extrapolated so that discoveries made in a larv ...
... present as general animal models for understanding vertebrate development. Larval fishes can serve as broadly applicable models because early stages of development are surprisingly similar across vertebrate taxa. These similarities can be exploited and extrapolated so that discoveries made in a larv ...
Time course of the development of motor behaviors in the zebrafish
... studied at different temperatures. The lower temperature used in earlier behavioral studies (25°C) results in a slower rate of growth (Kimmel et al., 1995) than that during morphological investigations (at the now standard temperature of 28.5°C), preventing a direct comparison of the two sets of obs ...
... studied at different temperatures. The lower temperature used in earlier behavioral studies (25°C) results in a slower rate of growth (Kimmel et al., 1995) than that during morphological investigations (at the now standard temperature of 28.5°C), preventing a direct comparison of the two sets of obs ...
Nat Methods 2015 Yu - Xiaokun Shu - University of California, San
... some of the many BphP sequences in protein sequence databases might be monomeric in the $BphP form, which is likely to occur if the protein lacks strong hydrophobic interactions at the putative dimer interface. In contrast, in IFP1.4’s parent, DrBphP13, the dimer interface includes several residues ...
... some of the many BphP sequences in protein sequence databases might be monomeric in the $BphP form, which is likely to occur if the protein lacks strong hydrophobic interactions at the putative dimer interface. In contrast, in IFP1.4’s parent, DrBphP13, the dimer interface includes several residues ...
Zebrafish primary neurons initiate expression of the
... old parts of the brain. In the chicken spinal cord Isl-1 has been identified as the earliest known marker of developing motor neurons and the onset of Isl-1 expression in the motor neurons can be ...
... old parts of the brain. In the chicken spinal cord Isl-1 has been identified as the earliest known marker of developing motor neurons and the onset of Isl-1 expression in the motor neurons can be ...
Molecular mechanisms of floor plate formation and neural patterning
... The vertebrate spinal cord is composed of billions of neurons and glia cells, which are formed in a highly coordinated manner during early neurogenesis. Specification of these cells at distinct positions along the dorsoventral (DV) axis of the developing spinal cord is controlled by a ventrally loca ...
... The vertebrate spinal cord is composed of billions of neurons and glia cells, which are formed in a highly coordinated manner during early neurogenesis. Specification of these cells at distinct positions along the dorsoventral (DV) axis of the developing spinal cord is controlled by a ventrally loca ...
Mutations affecting the development of the embryonic zebrafish brain
... dorsal view. Note the reduced medial expression domain of pax[zf-b] (arrowhead). (C,D) Expression of pax[zf-b] at the midbrainhindbrain boundary (arrow) and pax6 (forebrain, eye anlage and hindbrain) in wild-type (C) and spgm216 mutant (D) embryos at the 10-somites stage; dorsal view. (E,F) Lateral ...
... dorsal view. Note the reduced medial expression domain of pax[zf-b] (arrowhead). (C,D) Expression of pax[zf-b] at the midbrainhindbrain boundary (arrow) and pax6 (forebrain, eye anlage and hindbrain) in wild-type (C) and spgm216 mutant (D) embryos at the 10-somites stage; dorsal view. (E,F) Lateral ...
atterning the nervous system through development and evolution: a
... connectivity illuminates our understanding of what fear is. It is well known, of course, that the choice of a particular behavior at a particular time is based both on emotional input (affective value) and on past experience. In this context, Okamoto reminded us of the classical experiments where hu ...
... connectivity illuminates our understanding of what fear is. It is well known, of course, that the choice of a particular behavior at a particular time is based both on emotional input (affective value) and on past experience. In this context, Okamoto reminded us of the classical experiments where hu ...
A zebrafish model of CLN2 disease is deficient in
... impractical to generate large numbers of animals for drug discovery. Therefore assessment of early disease pathogenesis has not been performed and high-throughput drug screening for therapeutics development is expensive. An alternative model organism, that develops externally, is relatively cheap to ...
... impractical to generate large numbers of animals for drug discovery. Therefore assessment of early disease pathogenesis has not been performed and high-throughput drug screening for therapeutics development is expensive. An alternative model organism, that develops externally, is relatively cheap to ...
Chapter 7/8-Animal Biotechnology
... • Only 5% or less of the treated eggs become transgenic progeny • Need to check mouse pups for DNA (by PCR or Southerns), RNA (by northerns or RT-PCR), and protein (by western or by some ...
... • Only 5% or less of the treated eggs become transgenic progeny • Need to check mouse pups for DNA (by PCR or Southerns), RNA (by northerns or RT-PCR), and protein (by western or by some ...
Article Full Text PDF
... Generation of field effects and of chemical inhibition in the goldfish, a brief summary We found that as in the goldfish, the M-cell collateral inhibitory network of the zebrafish produces both electrical and chemical inhibitions mediated by the same set of interneurons. Before describing the result ...
... Generation of field effects and of chemical inhibition in the goldfish, a brief summary We found that as in the goldfish, the M-cell collateral inhibitory network of the zebrafish produces both electrical and chemical inhibitions mediated by the same set of interneurons. Before describing the result ...
Learning and memory in zebrafish larvae
... the response of an animal to repeated presentations of a stimulus of fixed intensity or strength gradually declines; furthermore, this decline is not due to sensory adaptation, motor fati, or injury (Thompson and Spencer, 1966; Rankin et al., 2009). Despite habituation’s simplicity and apparent ubiq ...
... the response of an animal to repeated presentations of a stimulus of fixed intensity or strength gradually declines; furthermore, this decline is not due to sensory adaptation, motor fati, or injury (Thompson and Spencer, 1966; Rankin et al., 2009). Despite habituation’s simplicity and apparent ubiq ...
Proper migration and axon outgrowth of zebrafish
... information from attractive and repulsive cues and growth promoting adhesive environments. Cell-cell and cell-substrate interactions via adhesion molecules, especially by molecules belonging to the immunoglobulin superfamily, have been shown to be crucial for nervous system development (Maness and S ...
... information from attractive and repulsive cues and growth promoting adhesive environments. Cell-cell and cell-substrate interactions via adhesion molecules, especially by molecules belonging to the immunoglobulin superfamily, have been shown to be crucial for nervous system development (Maness and S ...
RFC_Cp_C_Wyart_def_EUK-v
... generate electrical oscillations. In order to understand the functioning and modulation of the spinal locomotor network, Claire Wyart’s team studies motor activity in the zebrafish. This transparent vertebrate species is particularly suited to optogenetics, an innovative technology that allows stimu ...
... generate electrical oscillations. In order to understand the functioning and modulation of the spinal locomotor network, Claire Wyart’s team studies motor activity in the zebrafish. This transparent vertebrate species is particularly suited to optogenetics, an innovative technology that allows stimu ...
ZFIN5.08
... Curate data from zebrafish publications for mutant and morphant phenotypes into ZFIN and OBD using EQ syntax ...
... Curate data from zebrafish publications for mutant and morphant phenotypes into ZFIN and OBD using EQ syntax ...
Optogenetics in a transparent animal: circuit function in the larval
... large populations of neurons and causally testing their role by modifying their electrical properties and perturbing their spiking patterns. The use of light both for imaging and manipulation facilitates non-invasive access following the expression of transgene-derived proteins that either emit phot ...
... large populations of neurons and causally testing their role by modifying their electrical properties and perturbing their spiking patterns. The use of light both for imaging and manipulation facilitates non-invasive access following the expression of transgene-derived proteins that either emit phot ...
Zebrafish and motor control over the last decade
... combination of strengths that are allowing studies that are very difficult or impossible in other models. The two major assets of the zebrafish model are its transparency and its genetic accessibility. The transparency allows optical approaches to the study of structure and function in the intact an ...
... combination of strengths that are allowing studies that are very difficult or impossible in other models. The two major assets of the zebrafish model are its transparency and its genetic accessibility. The transparency allows optical approaches to the study of structure and function in the intact an ...
Drosophila as a model to study mechanisms underlying alcohol
... Laboratory: Center for Molecular Medicine, 4th floor, Robert-Koch-Str. 21, Cologne As a group based at the Institute of Human Genetics, the Wirth lab is involved in gene identification particularly in finding genes implicated in motor neuron diseases, such as spinal muscular atrophy but also of othe ...
... Laboratory: Center for Molecular Medicine, 4th floor, Robert-Koch-Str. 21, Cologne As a group based at the Institute of Human Genetics, the Wirth lab is involved in gene identification particularly in finding genes implicated in motor neuron diseases, such as spinal muscular atrophy but also of othe ...
Zebrafish
The zebrafish (Danio rerio) is a tropical freshwater fish belonging to the minnow family (Cyprinidae) of the order Cypriniformes. Native to the Himalayan region, it is a popular aquarium fish, frequently sold under the trade name zebra danio. The zebrafish is also an important and widely-used vertebrate model organism in scientific research, and was the first vertebrate to be cloned. It is particularly notable for its regenerative abilities, and has been modified by researchers to produce several transgenic strains.