instructions on the annotation of pdf files
... nACO(Nt) < ACO(Ct)/nACO(Ct) < ACO(Nt) are 1.7, 1.5, and 1.8 for the 19, 28, and 44 respective members of the a, b, a/b, and a+b classes in CATH, and 1.8, 1.3, and 1.4 for the 31, 46, and 88 members of these classes in SCOP. Importantly, the bias for ACO values of the N-terminal domains in two-domain ...
... nACO(Nt) < ACO(Ct)/nACO(Ct) < ACO(Nt) are 1.7, 1.5, and 1.8 for the 19, 28, and 44 respective members of the a, b, a/b, and a+b classes in CATH, and 1.8, 1.3, and 1.4 for the 31, 46, and 88 members of these classes in SCOP. Importantly, the bias for ACO values of the N-terminal domains in two-domain ...
bi6103-20feb04 - NUS School of Computing
... • PSORT-B generates all signal/features in a bottom-up way, then integrate them for decision making using Bayesian Network • Machine learning “beats” human expert? Probably the number of features/rules needed is too much/complicated Copyright 2003 limsoon wong ...
... • PSORT-B generates all signal/features in a bottom-up way, then integrate them for decision making using Bayesian Network • Machine learning “beats” human expert? Probably the number of features/rules needed is too much/complicated Copyright 2003 limsoon wong ...
Structure and Function of the Groucho Gene Family and Encoded
... TTTCAG in the genomic sequence abutting the short 5’ untranslated region TAAAA (Pflugrad et al., 1997). In humans, there are four Groucho (TLE1, TLE2, TLE3 and TLE4) genes and one AES gene. We previously reported that human TLE3 and AES genes are located on human chromosomes 15 and 19, respectively ...
... TTTCAG in the genomic sequence abutting the short 5’ untranslated region TAAAA (Pflugrad et al., 1997). In humans, there are four Groucho (TLE1, TLE2, TLE3 and TLE4) genes and one AES gene. We previously reported that human TLE3 and AES genes are located on human chromosomes 15 and 19, respectively ...
Fractionation of rice glutelin polypeptides using gel filtration
... would likely confound attempts to further characterize or fractionate the individual polypeptides. Gel filtration chromatography, using Sepharose CL-6B, was effective for partially purifying crude glutelin extracts and obtaining preparative samplesof dissociatedglutelin polypeptides suitable for fur ...
... would likely confound attempts to further characterize or fractionate the individual polypeptides. Gel filtration chromatography, using Sepharose CL-6B, was effective for partially purifying crude glutelin extracts and obtaining preparative samplesof dissociatedglutelin polypeptides suitable for fur ...
PDF
... The competence to undergo fertilization and early embryonic development is not acquired by mammalian oocytes until the completion of a final phase of differentiation immediately before ovulation. During this phase, referred to as the maturation period, many intracellular changes occur, including a r ...
... The competence to undergo fertilization and early embryonic development is not acquired by mammalian oocytes until the completion of a final phase of differentiation immediately before ovulation. During this phase, referred to as the maturation period, many intracellular changes occur, including a r ...
Factor VIlI-Related Protein Circulates in Normal
... available after the immobilization. The basic approach has a rather wide potential for application and has been of special antigenic ...
... available after the immobilization. The basic approach has a rather wide potential for application and has been of special antigenic ...
Translation
... endomembrane system and proteins that are secreted from the cell • Ribosomes are identical and can switch from free to bound Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
... endomembrane system and proteins that are secreted from the cell • Ribosomes are identical and can switch from free to bound Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
Diapositiva 1 - ASCRS/ASOA 2008
... right and left eyes of a single subject. All the normal electrophoretic bands are clearly more intense (increased evaporation?) ...
... right and left eyes of a single subject. All the normal electrophoretic bands are clearly more intense (increased evaporation?) ...
TRYPSIN / LYS
... Specificity: Serine endopeptidase that specifically hydrolyzes proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. Optimum pH: 8.0 Description Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of l ...
... Specificity: Serine endopeptidase that specifically hydrolyzes proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. Optimum pH: 8.0 Description Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of l ...
MICROSTRUCTURE OF PROTEIN BODIES IN MARAMA BEAN SPECIES
... beans may be due to differences in solubility behaviour of their proteins in Proteinase K buffer. ...
... beans may be due to differences in solubility behaviour of their proteins in Proteinase K buffer. ...
Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein
... forms of ISG15 [11]. ISG15 and other protein post-translational modifiers such as ubiquitin (Ub) and small ubiquitin-like modifier (SUMO) are central in fine-tuning antiviral innate immune responses and participate in regulating activity, stability, affinity and location of many signalling proteins ...
... forms of ISG15 [11]. ISG15 and other protein post-translational modifiers such as ubiquitin (Ub) and small ubiquitin-like modifier (SUMO) are central in fine-tuning antiviral innate immune responses and participate in regulating activity, stability, affinity and location of many signalling proteins ...
Cell-Free (In Vitro) Protein Expression
... Results 1. Asr1 binds directly to the RNAP II CTD in a ...
... Results 1. Asr1 binds directly to the RNAP II CTD in a ...
The DsbA Signal Sequence Directs Efficient
... DsbAss was significantly more hydrophobic than the other two signal sequences (data not shown) (see Materials and Methods). Since at about this time Lee and Bernstein (17) reported that they had engineered cleavable signal sequences with higher hydrophobicity than normal and that proteins attached t ...
... DsbAss was significantly more hydrophobic than the other two signal sequences (data not shown) (see Materials and Methods). Since at about this time Lee and Bernstein (17) reported that they had engineered cleavable signal sequences with higher hydrophobicity than normal and that proteins attached t ...
Full-Text PDF
... inflammation is the most important part in order to discover effective and specific antibiotics for H. pylori. In this regard, the structural and functional study of H. pylori proteins should be the most important point for developing antibiotics and can provide clues to help cope with new antibioti ...
... inflammation is the most important part in order to discover effective and specific antibiotics for H. pylori. In this regard, the structural and functional study of H. pylori proteins should be the most important point for developing antibiotics and can provide clues to help cope with new antibioti ...
Introduction to Database Searching using MASCOT
... molecular mass constraint. When this is done, the tag is effectively allowed to float within the candidate sequence, so that a match is possible even if there is a difference in the calculated mass to one side or the other of the tag. This is one of the few ways of getting a match to a peptide when ...
... molecular mass constraint. When this is done, the tag is effectively allowed to float within the candidate sequence, so that a match is possible even if there is a difference in the calculated mass to one side or the other of the tag. This is one of the few ways of getting a match to a peptide when ...
supp - Springer Static Content Server
... HnRNP F is an RNA binding protein belonging to the HnRNP H protein family that contains four highly homologous members, namely HnRNP H, HnRNP H’, HnRNP F, and HnRNP 2H9. These proteins contain two (2H9) or three (H, H’, and F) RNA recognition motifs (RRM) and two glycine rich auxiliary domains (Hono ...
... HnRNP F is an RNA binding protein belonging to the HnRNP H protein family that contains four highly homologous members, namely HnRNP H, HnRNP H’, HnRNP F, and HnRNP 2H9. These proteins contain two (2H9) or three (H, H’, and F) RNA recognition motifs (RRM) and two glycine rich auxiliary domains (Hono ...
Gene Section BRD4 (bromodomain containing 4) Atlas of Genetics and Cytogenetics
... A striking feature of BRD4 is its association with euchromatic regions of mitotic chromosomes. By this association, the protein exerts its function as regulator of cell cycle progression from G2 to M but also in the G1 to S transition. It has also been suggested that the association of BRD4 to chrom ...
... A striking feature of BRD4 is its association with euchromatic regions of mitotic chromosomes. By this association, the protein exerts its function as regulator of cell cycle progression from G2 to M but also in the G1 to S transition. It has also been suggested that the association of BRD4 to chrom ...
AMIN domains have a predicted role in localization of diverse
... whose PilQ protein also contains AMIN domains: even though PilQ itself has not been localized, several other proteins involved in the biogenesis of type IV pilus have been shown to be polar in this species (Chiang et al., 2005); and (iv) Evidence from the Neisseria PilQ protein suggests that its N-t ...
... whose PilQ protein also contains AMIN domains: even though PilQ itself has not been localized, several other proteins involved in the biogenesis of type IV pilus have been shown to be polar in this species (Chiang et al., 2005); and (iv) Evidence from the Neisseria PilQ protein suggests that its N-t ...
bioinformatics - Noble Research Lab
... These methods include the yeast two-hybrid screen and methods based on mass spectrometry (see von Mering et al., 2002 and references therein). The data obtained by these methods are partial: each experimental assay can identify only a subset of the interactions, and it has been estimated that for th ...
... These methods include the yeast two-hybrid screen and methods based on mass spectrometry (see von Mering et al., 2002 and references therein). The data obtained by these methods are partial: each experimental assay can identify only a subset of the interactions, and it has been estimated that for th ...
chromatography - Bio-Rad
... A human serum sample was loaded onto a BioLogic DuoFlow chromatography system configured to use both an Affi-Gel Blue and an Affi-Gel protein A column for removal of albumin and IgG, respectively (Figure 1). Since protein A media can be costly, the relative purification efficiency achieved using dif ...
... A human serum sample was loaded onto a BioLogic DuoFlow chromatography system configured to use both an Affi-Gel Blue and an Affi-Gel protein A column for removal of albumin and IgG, respectively (Figure 1). Since protein A media can be costly, the relative purification efficiency achieved using dif ...
Changes in Typical Organelles in Developing Cotyledons of Soybean
... 3E) and then disappeared rapidly as seeds reached maturity (Fig. 3F). The formation of vacuoles smaller than the large centrally located vacuole, was observed from about 25 OAF and so me times the organelles found by Kaizuma et al. ( 1981) were also recognized (Figs. 3C, 30) . These organelles were ...
... 3E) and then disappeared rapidly as seeds reached maturity (Fig. 3F). The formation of vacuoles smaller than the large centrally located vacuole, was observed from about 25 OAF and so me times the organelles found by Kaizuma et al. ( 1981) were also recognized (Figs. 3C, 30) . These organelles were ...
Bioinformatic Software in Web
... glass microscope slide39. The immobilized GST fusion proteins were detected using a labeled antibody against GST. (MacBeath G. Nat Genet 2002 Dec;32 Suppl 2:526-32 ) ...
... glass microscope slide39. The immobilized GST fusion proteins were detected using a labeled antibody against GST. (MacBeath G. Nat Genet 2002 Dec;32 Suppl 2:526-32 ) ...
Inhibition of Protein Kinases by Balanol: Specificity within the Serine
... and 65.2 nM, respectively (Fig. 2D). The Ki value of 100deoxybalanol is 2910 nM toward PKCa and 550 nM toward PKCbII. The Ki values of 140-decarboxybalanol for PKCa and PKCbII are 11,680 and 2000 nM (Fig. 2D). Thus, elimination of either one of these functional groups greatly affects the affinity of ...
... and 65.2 nM, respectively (Fig. 2D). The Ki value of 100deoxybalanol is 2910 nM toward PKCa and 550 nM toward PKCbII. The Ki values of 140-decarboxybalanol for PKCa and PKCbII are 11,680 and 2000 nM (Fig. 2D). Thus, elimination of either one of these functional groups greatly affects the affinity of ...
prosite.excerpt
... origins, including meat products, dairy products and vegetables. Class IIa bacteriocins are all cationic, display anti-Listeria activity, and kill target cells by permeabilizing the cell membrane [1-3]. Class IIa bacteriocins contain between 37 and 48 residues. Based on their primary structures, the ...
... origins, including meat products, dairy products and vegetables. Class IIa bacteriocins are all cationic, display anti-Listeria activity, and kill target cells by permeabilizing the cell membrane [1-3]. Class IIa bacteriocins contain between 37 and 48 residues. Based on their primary structures, the ...
Protein folding
Protein folding is the process by which a protein structure assumes its functional shape or conformation. It is the physical process by which a polypeptide folds into its characteristic and functional three-dimensional structure from random coil.Each protein exists as an unfolded polypeptide or random coil when translated from a sequence of mRNA to a linear chain of amino acids. This polypeptide lacks any stable (long-lasting) three-dimensional structure (the left hand side of the first figure). Amino acids interact with each other to produce a well-defined three-dimensional structure, the folded protein (the right hand side of the figure), known as the native state. The resulting three-dimensional structure is determined by the amino acid sequence (Anfinsen's dogma). Experiments beginning in the 1980s indicate the codon for an amino acid can also influence protein structure.The correct three-dimensional structure is essential to function, although some parts of functional proteins may remain unfolded, so that protein dynamics is important. Failure to fold into native structure generally produces inactive proteins, but in some instances misfolded proteins have modified or toxic functionality. Several neurodegenerative and other diseases are believed to result from the accumulation of amyloid fibrils formed by misfolded proteins. Many allergies are caused by incorrect folding of some proteins, because the immune system does not produce antibodies for certain protein structures.