Modern affinity reagents: Recombinant antibodies and aptamers
... line of antibody-producing cells) antibodies are used in research. Monoclonal antibodies are defined by their capacity to selectively bind a single antigen. 1.2. Historical methods of monoclonal antibody discovery and production Monoclonal antibodies are generated using either animal or recombinant D ...
... line of antibody-producing cells) antibodies are used in research. Monoclonal antibodies are defined by their capacity to selectively bind a single antigen. 1.2. Historical methods of monoclonal antibody discovery and production Monoclonal antibodies are generated using either animal or recombinant D ...
Introduction to Immunoassays
... • The process of making an antiserum begins by injecting a solution that contains the antigen of interest into an animal. This antigen of interest is sometimes called an immunogen, because it can stimulate an immune response. Over time, and in some cases with multiple injections, the immune system o ...
... • The process of making an antiserum begins by injecting a solution that contains the antigen of interest into an animal. This antigen of interest is sometimes called an immunogen, because it can stimulate an immune response. Over time, and in some cases with multiple injections, the immune system o ...
Curriculum Vitae
... 42. Oseroff C, Peters B, Pasquetto V, Moutaftsi M, Sidney J, Panchanathan V, Tscharke DC, Maillere B, Grey H, Sette A. Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus western reserve. J Immunol. 2008 Jun 1;180(11):7193. PMCID: PMC2533852 43. Sette A, M ...
... 42. Oseroff C, Peters B, Pasquetto V, Moutaftsi M, Sidney J, Panchanathan V, Tscharke DC, Maillere B, Grey H, Sette A. Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus western reserve. J Immunol. 2008 Jun 1;180(11):7193. PMCID: PMC2533852 43. Sette A, M ...
Analysis of the roles of Interleukin 15 and CD4+ T cells specific of a
... In physiological conditions, robust immunological mechanisms avoid adverse responses to food antigens. In contrast, in celiac disease that affects about 1% of Western populations, exposure to dietary gluten of genetically predisposed HLA-DQ2.5/ DQ8 individuals triggers a chronic small intestinal ent ...
... In physiological conditions, robust immunological mechanisms avoid adverse responses to food antigens. In contrast, in celiac disease that affects about 1% of Western populations, exposure to dietary gluten of genetically predisposed HLA-DQ2.5/ DQ8 individuals triggers a chronic small intestinal ent ...
Lymphoid follicles in (very) severe COPD: beneficial or harmful? REVIEW
... been demonstrated to be oligoclonal in nature [11], suggesting antigen-specific induction of the B-cell follicles. In this study, 10 lymphoid follicles were isolated by laser microdissection from the lung tissue of eight patients with COPD [11]. Sequence analysis of the Ig rearrangements revealed th ...
... been demonstrated to be oligoclonal in nature [11], suggesting antigen-specific induction of the B-cell follicles. In this study, 10 lymphoid follicles were isolated by laser microdissection from the lung tissue of eight patients with COPD [11]. Sequence analysis of the Ig rearrangements revealed th ...
Plaque reduction test: an alternative method to assess specific
... 3.2. Sequences analysis of selected heptapeptides After three rounds of biopanning, individual phage clones were isolated and characterized by DNA sequencing. The number of pfu obtained after each round of biopanning increased gradually, indicating that the biopanning selections were successful. The ...
... 3.2. Sequences analysis of selected heptapeptides After three rounds of biopanning, individual phage clones were isolated and characterized by DNA sequencing. The number of pfu obtained after each round of biopanning increased gradually, indicating that the biopanning selections were successful. The ...
Full-Text PDF
... recognized by distinct receptors. The effector responses triggered lead to a chronic tissue inflammation. DsDNA autoantibodies are the most studied as well as the first in being characterized for its pathogenic role in Lupus nephritis. However, others are of growing interest since they have been ass ...
... recognized by distinct receptors. The effector responses triggered lead to a chronic tissue inflammation. DsDNA autoantibodies are the most studied as well as the first in being characterized for its pathogenic role in Lupus nephritis. However, others are of growing interest since they have been ass ...
CD4+ T-Cell-Independent Secondary Immune Responses to
... In order to assess whether infection with Pneumocystis generates Pneumocystis-specific bone marrow plasma cells (BMPCs), BMPCs were enumerated using flow cytometry and cultured. BMPCs (CD45R−CD138+) are long-lived cells that secrete neutralizing antibody after exposure to antigen. As terminally diff ...
... In order to assess whether infection with Pneumocystis generates Pneumocystis-specific bone marrow plasma cells (BMPCs), BMPCs were enumerated using flow cytometry and cultured. BMPCs (CD45R−CD138+) are long-lived cells that secrete neutralizing antibody after exposure to antigen. As terminally diff ...
Vaccination with recombinant fusion proteins incorporating Toll
... lar mechanisms governing the regulation of the host immune response. Janeway originally postulated that the recognition of pathogens by the host was mediated by specific receptors on the surface of antigen presenting cells (APC) [9]. This hypothesis has now been supported by the identification of 11 ...
... lar mechanisms governing the regulation of the host immune response. Janeway originally postulated that the recognition of pathogens by the host was mediated by specific receptors on the surface of antigen presenting cells (APC) [9]. This hypothesis has now been supported by the identification of 11 ...
The Immune System
... system is composed of many interdependent cell types that collectively protect the body from bacterial parasitic fungal viral infections and from the, immune system diseases disorders function - the role of the immune system a collection of structures and processes within the body is to protect agai ...
... system is composed of many interdependent cell types that collectively protect the body from bacterial parasitic fungal viral infections and from the, immune system diseases disorders function - the role of the immune system a collection of structures and processes within the body is to protect agai ...
History of Immunology
... disease that the sick and the dying found most compassion. These knew what it was from experience, and had now no fear for themselves; for the same man was never attacked twice - never at least fatally” Resistance to re-infection-Immunity Department of Immunology ...
... disease that the sick and the dying found most compassion. These knew what it was from experience, and had now no fear for themselves; for the same man was never attacked twice - never at least fatally” Resistance to re-infection-Immunity Department of Immunology ...
fulltext
... T-cells, like B-cells, originate from the bone marrow (BM), but they mature in the thymus. In the thymus the T-cells start to express their antigen binding receptor, the T-cell receptor (TCR). The TCR recognizes protein antigens that are displayed as peptides by major histocompability complex (MHC) ...
... T-cells, like B-cells, originate from the bone marrow (BM), but they mature in the thymus. In the thymus the T-cells start to express their antigen binding receptor, the T-cell receptor (TCR). The TCR recognizes protein antigens that are displayed as peptides by major histocompability complex (MHC) ...
PDF - Journal of Veterinary Science
... causative agents of AR. In this study, we tried to solve the problem by reformulating experimental vaccines. For this purpose, 4 different groups were designed based on the vaccine components of the causative agents of AR, B. bronchiseptica and P. multocida 4:D, and animals were immunized using diff ...
... causative agents of AR. In this study, we tried to solve the problem by reformulating experimental vaccines. For this purpose, 4 different groups were designed based on the vaccine components of the causative agents of AR, B. bronchiseptica and P. multocida 4:D, and animals were immunized using diff ...
Monoclonal Antibodies Binding Renal Renin
... Since each antiserum is comprised of a different mixture of antibodies varying in both specificity and affinity," comparative experiments among different laboratories is difficult. We have noted an additional problem. The effectiveness of a mixed immunoglobulin population in inhibiting the enzymatic ...
... Since each antiserum is comprised of a different mixture of antibodies varying in both specificity and affinity," comparative experiments among different laboratories is difficult. We have noted an additional problem. The effectiveness of a mixed immunoglobulin population in inhibiting the enzymatic ...
The use of antibodies in the treatment of infectious diseases
... (boxes) have enabled the shift from polyclonal serum towards monoclonal and increasingly more human antibodies. ...
... (boxes) have enabled the shift from polyclonal serum towards monoclonal and increasingly more human antibodies. ...
NOD2 Variants and Antibody Response to Microbial Antigens in
... A hyperresponsive adaptive immunologic response to microbial antigens has been characterized in patients with CD and is believed to be reflective of the underlying immunopathogenesis of this disorder. Measures of this adaptive immunologic response include antibodies to oligomannan (anti–Saccharomyce ...
... A hyperresponsive adaptive immunologic response to microbial antigens has been characterized in patients with CD and is believed to be reflective of the underlying immunopathogenesis of this disorder. Measures of this adaptive immunologic response include antibodies to oligomannan (anti–Saccharomyce ...
Common Features of Mucosal and Peripheral Antibody Responses
... cific IgG and IgA responses in sera and mucosal secretions at the peak time point (P ⬍ 0.01; paired t test), and these responses declined by approximately 0.5 log (range, 0.17 to 0.57 log; median, 0.54) by 20 to 24 weeks following the final immunization (Fig. 2A). Consistent with prior reports (5), ...
... cific IgG and IgA responses in sera and mucosal secretions at the peak time point (P ⬍ 0.01; paired t test), and these responses declined by approximately 0.5 log (range, 0.17 to 0.57 log; median, 0.54) by 20 to 24 weeks following the final immunization (Fig. 2A). Consistent with prior reports (5), ...
Advances in siRNA delivery to T-cells: potential clinical applications
... have significant side effects. Most importantly, a cure is still not available [14,17]. It is clear, therefore, that new and more refined therapies that modulate T-cell function are urgently needed. Silencing of gene expression in T-cells using siRNAs is considered an attractive therapeutic strategy ...
... have significant side effects. Most importantly, a cure is still not available [14,17]. It is clear, therefore, that new and more refined therapies that modulate T-cell function are urgently needed. Silencing of gene expression in T-cells using siRNAs is considered an attractive therapeutic strategy ...
Really Essential Medical Immunology Arthur Rabson
... Complement has a range of defensive biological functions, 9 Complement can mediate an acute inflammatory reaction, 10 Macrophages can also do it, 10 Humoral mechanisms provide a second defensive strategy, 11 Acute phase proteins increase in response to infection, 12 Extracellular killing, 13 Natural ...
... Complement has a range of defensive biological functions, 9 Complement can mediate an acute inflammatory reaction, 10 Macrophages can also do it, 10 Humoral mechanisms provide a second defensive strategy, 11 Acute phase proteins increase in response to infection, 12 Extracellular killing, 13 Natural ...
Rheumatoid factors: what`s new?
... excess immune complex production. In particular, HERVs may contribute to the pathogenesis of disease, possibly by acting in concert with other infectious agents [47], or as potential transducing agents that enable other environmental/hormonal triggers to alter the immune system [48]. EBV is a classi ...
... excess immune complex production. In particular, HERVs may contribute to the pathogenesis of disease, possibly by acting in concert with other infectious agents [47], or as potential transducing agents that enable other environmental/hormonal triggers to alter the immune system [48]. EBV is a classi ...
Human Immunodeficiency Virus (HIV) Tat
... that range. These data suggest that progression to AIDS is accompanied by a decline in HIV-positive sera of a constant component of normal sera: a set of IgM antibodies reactive with HIV Tat protein. HIV-negative Sera Are Not Reactive with HIV Proteins Other than Tat. Further creditation for the spe ...
... that range. These data suggest that progression to AIDS is accompanied by a decline in HIV-positive sera of a constant component of normal sera: a set of IgM antibodies reactive with HIV Tat protein. HIV-negative Sera Are Not Reactive with HIV Proteins Other than Tat. Further creditation for the spe ...
antibody structure and function
... respond to artificial antigens that do not exist in nature (Fast Focus 1). Because the amino acid sequence differs in the arms of various antibody molecules, each different antibody can bind specifically to 1We use the term antigen, even though immunogen may be more accurate at times, as explained i ...
... respond to artificial antigens that do not exist in nature (Fast Focus 1). Because the amino acid sequence differs in the arms of various antibody molecules, each different antibody can bind specifically to 1We use the term antigen, even though immunogen may be more accurate at times, as explained i ...
... molecules by themselves can seldom produce specific circulating antibodies. However, many small molecules may change the antigenic properties of a larger molecule, i.e. a protein, when covalently coupled to it (haptens). The resulting antibodies are partially directed against the small molecule. Imm ...
Exploring the Homeostatic and Sensory Roles of the Immune System
... organisms and essential for host fitness. Although it may appear controversial to have two complex systems in charge of the same function, which already exists in the host, the nervous and immune systems are not redundant. These systems mediate interactions between host and environment that exceed i ...
... organisms and essential for host fitness. Although it may appear controversial to have two complex systems in charge of the same function, which already exists in the host, the nervous and immune systems are not redundant. These systems mediate interactions between host and environment that exceed i ...
and B-‐cell Responses against Human Cytomegalovirus after
... PhD thesis I have investigated how specific T- and B-lymphocyte responses against CMV reconstitute after HSCT. In my thesis I will therefore explain both how our studies were preformed and what they have shown and furthermore I will give the reader some background in the fields of immunology, basic ...
... PhD thesis I have investigated how specific T- and B-lymphocyte responses against CMV reconstitute after HSCT. In my thesis I will therefore explain both how our studies were preformed and what they have shown and furthermore I will give the reader some background in the fields of immunology, basic ...
Gluten immunochemistry
The immunochemistry of Triticeae glutens is important in several inflammatory diseases. It can be subdivided into innate responses (direct stimulation of immune system), class II mediated presentation (HLA DQ), class I meditiated stimulation of killer cells, and antibody recognition. The responses to gluten proteins and polypeptide regions differs according to the type of gluten sensitivity. The response is also dependent on the genetic makeup of the human leukocyte antigen genes. In gluten sensitive enteropathy, there are 4 types of recognition, innate immunity (a form of cellular immunity priming), HLA-DQ, and antibody recognition of gliadin and transglutaminase. With idiopathic gluten sensitivity only antibody recognition to gliadin has been resolved. In wheat allergy, the response pathways are mediated through IgE against other wheat proteins and other forms of gliadin.