Slide 1
... Protein Transport (Prokaryotes) Protein transport is more complex in eukaryotes Transport is also important in prokaryotes eg. secreted proteins Targeting proteins to periplasm for disulfide bond formation Periplasmic targeting requires a signal sequence (signal peptide) ...
... Protein Transport (Prokaryotes) Protein transport is more complex in eukaryotes Transport is also important in prokaryotes eg. secreted proteins Targeting proteins to periplasm for disulfide bond formation Periplasmic targeting requires a signal sequence (signal peptide) ...
Inherited platelet disorders: towards DNA-based
... Biobank of 500,000 healthy individuals92. Both projects link genotypes with health and social care records and will lead to a better understanding of the relationship between variants and diseases. In particular, they will allow more accurate assignment of pathogenicity to rare variants underlying t ...
... Biobank of 500,000 healthy individuals92. Both projects link genotypes with health and social care records and will lead to a better understanding of the relationship between variants and diseases. In particular, they will allow more accurate assignment of pathogenicity to rare variants underlying t ...
How oleic acid in olive oil reduces blood pressure
... reductions were observed (data not shown), indicating that the effect cannot be attributed to the PKA inhibitor itself. In addition, this effect was time- and concentration-dependent. ...
... reductions were observed (data not shown), indicating that the effect cannot be attributed to the PKA inhibitor itself. In addition, this effect was time- and concentration-dependent. ...
Research data
... removal of the stop codon in the exon 2 of the Pdx1 gene and fusion of the open reading frames of this gene (orange boxes) to the fluorescent protein reporter gene BFP (light blue arrow). The PGK-driven Neomycin (neo)-resistant cassette, flanked by LoxP sites, was removed through Cre-recombinase-med ...
... removal of the stop codon in the exon 2 of the Pdx1 gene and fusion of the open reading frames of this gene (orange boxes) to the fluorescent protein reporter gene BFP (light blue arrow). The PGK-driven Neomycin (neo)-resistant cassette, flanked by LoxP sites, was removed through Cre-recombinase-med ...
Global gene expression analysis of human erythroid
... cytometry of peripheral blood mononucleocyte-derived erythroblasts. Cells were stained for 15 minutes at 4°C in 2% bovine serum albumin in HBSS and washed twice. CFU-E are CD36⫹, CD71⫹, and CD235a⫺/low. CD235a expression increases until the intermediate erythroblast stage, when it remains high and C ...
... cytometry of peripheral blood mononucleocyte-derived erythroblasts. Cells were stained for 15 minutes at 4°C in 2% bovine serum albumin in HBSS and washed twice. CFU-E are CD36⫹, CD71⫹, and CD235a⫺/low. CD235a expression increases until the intermediate erythroblast stage, when it remains high and C ...
The Sex-Inducing Pheromone and Wounding Trigger the Same Set
... The sex-inducing pheromone of the multicellular green alga Volvox carteri is a glycoprotein that triggers development of males and females at a concentration ,10216 M. By differential screening of a cDNA library, two novel genes were identified that are transcribed under the control of this pheromon ...
... The sex-inducing pheromone of the multicellular green alga Volvox carteri is a glycoprotein that triggers development of males and females at a concentration ,10216 M. By differential screening of a cDNA library, two novel genes were identified that are transcribed under the control of this pheromon ...
ARTICLE IN PRESS Small Ruminant Research R.C. Scheepers, E. van Marle-Köster
... -casein (kappa-casein) is of special interest as a milk protein polymorphism due to its known relationship with milk quality and composition. A number of allelic variants have been identified in goats. In this study, genetic variants of the -casein gene were evaluated in the South African Boer goat ...
... -casein (kappa-casein) is of special interest as a milk protein polymorphism due to its known relationship with milk quality and composition. A number of allelic variants have been identified in goats. In this study, genetic variants of the -casein gene were evaluated in the South African Boer goat ...
6. Global gene expression analysis of human erythroid progenitors.
... cytometry of peripheral blood mononucleocyte-derived erythroblasts. Cells were stained for 15 minutes at 4°C in 2% bovine serum albumin in HBSS and washed twice. CFU-E are CD36⫹, CD71⫹, and CD235a⫺/low. CD235a expression increases until the intermediate erythroblast stage, when it remains high and C ...
... cytometry of peripheral blood mononucleocyte-derived erythroblasts. Cells were stained for 15 minutes at 4°C in 2% bovine serum albumin in HBSS and washed twice. CFU-E are CD36⫹, CD71⫹, and CD235a⫺/low. CD235a expression increases until the intermediate erythroblast stage, when it remains high and C ...
Molecule of the Month on Hemoglobin
... Hemoglobin is a remarkable molecular machine that uses motion and small structural changes to regulate its action. Oxygen binding at the four heme sites in hemoglobin does not happen simultaneously. Once the first heme binds oxygen, it introduces small changes in the structure of the corresponding p ...
... Hemoglobin is a remarkable molecular machine that uses motion and small structural changes to regulate its action. Oxygen binding at the four heme sites in hemoglobin does not happen simultaneously. Once the first heme binds oxygen, it introduces small changes in the structure of the corresponding p ...
Evolution of Primate ABO Blood Group Genes
... There are three common alleles (A, B, and 0) at the human ABO blood group locus. We compared nucleotide sequences of these alleles, and relatively large numbers of nucleotide differences were found among them. These differences correspond to the divergence time of at least a few million years, which ...
... There are three common alleles (A, B, and 0) at the human ABO blood group locus. We compared nucleotide sequences of these alleles, and relatively large numbers of nucleotide differences were found among them. These differences correspond to the divergence time of at least a few million years, which ...
post-peer-review-non
... In this study we use liquid chromatography tandem mass spectrometry (LC-MS/MS), denovo sequencing and database searching to enable an accurate and convenient workflow to identify neopeptides (in selected proteoglycans; important to cartilage biology) released by either specific protease treatment of ...
... In this study we use liquid chromatography tandem mass spectrometry (LC-MS/MS), denovo sequencing and database searching to enable an accurate and convenient workflow to identify neopeptides (in selected proteoglycans; important to cartilage biology) released by either specific protease treatment of ...
Capture Compound Mass Spectrometry: A Technology for the
... Capture of CA II from HEK293 flow-through fraction with capture compound 8 As a representative example for capture and pullout experiments the procedure generating the results of Fig. 1B is given. SoftLink avidin resin (Promega) was washed and equilibrated three times with 20 mM HEPES (pH 7.2), and ...
... Capture of CA II from HEK293 flow-through fraction with capture compound 8 As a representative example for capture and pullout experiments the procedure generating the results of Fig. 1B is given. SoftLink avidin resin (Promega) was washed and equilibrated three times with 20 mM HEPES (pH 7.2), and ...
Protein - UMass Nutrition
... oz glass of low-fat milk? • 12 grams Incorrect. There is one gram of protein for each ounce of milk. The protein content is the same in skim, low fat and whole milk, only the fat content changes. Next Question ...
... oz glass of low-fat milk? • 12 grams Incorrect. There is one gram of protein for each ounce of milk. The protein content is the same in skim, low fat and whole milk, only the fat content changes. Next Question ...
Production of Human Erythropoietin in Transgenic Canola
... spinal marrow, ischemic brain damage, congestive cardiac diseases and neurological injuries. EPO has been shown to prevent apoptosis, balance inflammatory responses, fuel angiogenesis and stimulate the engagement of stem cells [5-8, 11]. As an endogenous cytokine, EPO possesses a specific structure ...
... spinal marrow, ischemic brain damage, congestive cardiac diseases and neurological injuries. EPO has been shown to prevent apoptosis, balance inflammatory responses, fuel angiogenesis and stimulate the engagement of stem cells [5-8, 11]. As an endogenous cytokine, EPO possesses a specific structure ...
Hemoglobinopathies and thalasemias – genetic basis and
... DNA sequences. This method is usually connected with RFLP technique (restriction fragment length polymorphism), in which restriction enzymes are used, cutting DNA at specific sequences. When the mutation in the globin gene removes recognition site in DNA sequence, the pattern of DNA fragments is dif ...
... DNA sequences. This method is usually connected with RFLP technique (restriction fragment length polymorphism), in which restriction enzymes are used, cutting DNA at specific sequences. When the mutation in the globin gene removes recognition site in DNA sequence, the pattern of DNA fragments is dif ...
Grobe AHA progress report ROUGH DRAFT
... mentor and I decided to change the focus of my work while still in the infant stages of my predoctoral work. We decided to shift my efforts toward understanding the physiological regulation (and importance in hypertension) of the newly discovered ACE2 gene, rather than focusing on physiological effe ...
... mentor and I decided to change the focus of my work while still in the infant stages of my predoctoral work. We decided to shift my efforts toward understanding the physiological regulation (and importance in hypertension) of the newly discovered ACE2 gene, rather than focusing on physiological effe ...
enzymatik control
... Lipase is an enzyme the body uses to break down fats in food so they can be absorbed in the intestines. Lipase is produced in the pancreas, mouth, and stomach. Pancreatic lipase, also known as pancreatic triacylglycerol lipase, is an enzymesecreted from the pancreas, and is the primary lipase enzyme ...
... Lipase is an enzyme the body uses to break down fats in food so they can be absorbed in the intestines. Lipase is produced in the pancreas, mouth, and stomach. Pancreatic lipase, also known as pancreatic triacylglycerol lipase, is an enzymesecreted from the pancreas, and is the primary lipase enzyme ...
Diuretics
... Loops require renal prostaglandins to work, therefore NSAIDs can interfere with diuresis ...
... Loops require renal prostaglandins to work, therefore NSAIDs can interfere with diuresis ...
Transcription factor networks in B-cell differentiation
... expression of a certain surface marker. Complete lack of Ig expression, as in Bprecursor ALL, suggest that the progenitor cell either has not completed IGrearangement or that the recombination event has failed to generate a functional IgH chain gene. The expression of intracellular IgH chain, as det ...
... expression of a certain surface marker. Complete lack of Ig expression, as in Bprecursor ALL, suggest that the progenitor cell either has not completed IGrearangement or that the recombination event has failed to generate a functional IgH chain gene. The expression of intracellular IgH chain, as det ...
SERIES 0CONTROVERSIES IN OCCUPATIONAL ASTHMA0 Number 3 in this Series
... combination of linkage and segregation analyses increases the power to determine genetic interactions. However, it also generates some disadvantages. For example, combining the two methods works better only for the analysis of quantitative traits. Epidemiological approaches. The epidemiological appr ...
... combination of linkage and segregation analyses increases the power to determine genetic interactions. However, it also generates some disadvantages. For example, combining the two methods works better only for the analysis of quantitative traits. Epidemiological approaches. The epidemiological appr ...
Regulation of Toxic Shock Syndrome Toxin
... in the production of 51.7 Ilg of toxin per mJ of culture supernatant of stationary cells. However, transformants derived from RN6390 produced 0.5 Ilg per ml of supernatant, even though the recombinant plasmid was stably maintained at an equivalent copy number in the two strains. The results indicate ...
... in the production of 51.7 Ilg of toxin per mJ of culture supernatant of stationary cells. However, transformants derived from RN6390 produced 0.5 Ilg per ml of supernatant, even though the recombinant plasmid was stably maintained at an equivalent copy number in the two strains. The results indicate ...
packet
... sequence that represents the allele for Normal Hemoglobin. The other group member should transcribe the mRNA from the DNA sequence that represents the allele for Sickle Cell hemoglobin. Write both mRNA sequences in section 3 of your poster. 2. Compare the mRNA that results from the transcription of ...
... sequence that represents the allele for Normal Hemoglobin. The other group member should transcribe the mRNA from the DNA sequence that represents the allele for Sickle Cell hemoglobin. Write both mRNA sequences in section 3 of your poster. 2. Compare the mRNA that results from the transcription of ...
In Vivo Search for Butyrate Responsive Sequences Using
... If BRE were present along the y gene promoter, mice carrying truncated constructs lacking these elements were expected to fail to respond to aABA by increasing y gene expression. Mice carrying y gene promoters containing a BRE were expected to respond to butyrate treatment by increasing y gene expre ...
... If BRE were present along the y gene promoter, mice carrying truncated constructs lacking these elements were expected to fail to respond to aABA by increasing y gene expression. Mice carrying y gene promoters containing a BRE were expected to respond to butyrate treatment by increasing y gene expre ...
GENE EXPRESSION: PROTEIN SYNTHESIS
... sequence that represents the allele for Normal Hemoglobin. The other group member should transcribe the mRNA from the DNA sequence that represents the allele for Sickle Cell hemoglobin. Write both mRNA sequences in section 3 of your poster. 2. Compare the mRNA that results from the transcription of ...
... sequence that represents the allele for Normal Hemoglobin. The other group member should transcribe the mRNA from the DNA sequence that represents the allele for Sickle Cell hemoglobin. Write both mRNA sequences in section 3 of your poster. 2. Compare the mRNA that results from the transcription of ...
Complement component 4
Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b; 4b can be further cleaved into 4c and 4d. C4a is an anaphylatoxin. C4b forms part of C3-convertase, in conjunction with 2a: C4b can bind CR1.