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DNA Similarities
DNA Similarities

... Suppose you could compare the total DNA sequences of various organisms (some billions of base pairs). How much similarity would you expect between a whale and a fish? A whale and a dog? A dog and a shrimp? A shrimp and a bacterium? As always, there are two types of similarity to be considered: analo ...
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... The process of copying DNA in a cell is called ____________________. In the first step, the twonucleotide chains separate. The point at which the two chains separate is called the _____________ _____________, and are separated by enzymes called ____________________. In the next step, enzymes called ...
CHAPTER 10 - Protein Synthesis The DNA genotype is expressed
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English Version

... 2. Profound grasp of the key knowledge of the structure and function of DNA and RNA. 3. Familiarity with the physio-chemical properties of DNA and the relation between these properties and the DNA structure. 4. To know the technique of isolation and purification of nucleic acids and DNA sequencing. ...
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DNA and Gene Expression

A. Nucleic Acid = polymer of nucleotides 1. nucleotide = molecule
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BIOLOGY-DNA replication, transcription, translation (DOC 98KB)
BIOLOGY-DNA replication, transcription, translation (DOC 98KB)

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L27- Cloning
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Practical molecular biology
Practical molecular biology

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̚Ꮈ̂ጯ Ⴧ̀঱٢Ϡ ྏྏᗟ

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Real-time polymerase chain reaction



A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.
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