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Gram-staining procedure
Gram-staining procedure

... decolorization while Gram negative bacteria do not retain the dye. In order to visualize the decolorized Gram negative cells a counterstain (safranin - a red stain) is used. In addition to Gram positive/ negative two other groups have been recognized: Gram nonreactive, do not stain or stain poorly b ...
Concept questions-lecture exam 1
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... When are the chromosomes visible? What causes them to be visible? 6. Define mitosis and explain its function. What happens to the chromosome number during this process? How does a diploid organism remain diploid and a haploid organism remain haploid? 7. Define meiosis and explain its function. When ...
acid-fast endospore and capsule stain
acid-fast endospore and capsule stain

... endospore form. Examples of bacteria that can form endospores include Bacillus and Clostridium. • Endospore formation process called sporogenesis. • An endospore is a dormant, tough, and nonreproductive structure produced by certain bacteria from the Firmicute phylum. ...
Chapter 27- Prokaryotes and the Origin of Metabolism
Chapter 27- Prokaryotes and the Origin of Metabolism

...  Some even used the oxidizing property of O2 to pull electrons down electron chains.  Some bacteria gave up photosynthesis to become solely chemoheterotrophs. ...
microbiology exam ii - Medical Mastermind Community
microbiology exam ii - Medical Mastermind Community

... 77. The proton motive force, pmf, has as a component the: A. Na+ concentration inside the cell versus that outside the cell B. difference in pH outside the cell versus that inside the cell C. K+ concentration outside the cell versus that inside the cell D. Ca2+ concentration inside the cell versus t ...
PROBIOTIC FOODS: HEALTHY WAY TO HEALTHY LIFE
PROBIOTIC FOODS: HEALTHY WAY TO HEALTHY LIFE

... alter the intestinal microflora balance in a positive way. Fructofibres® stimulate the proliferation of the health-promoting bacteria in the gastro-intestinal tract and encourage their metabolic selectivity. These effects promote a healthy digestive tract and increased resistance to infection. ...
Bacterial Meningitis - UNC
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... of several antibiotics like penicillin and cephalosporins  Attack peptioglycans in bacterial cell walls ...
Read the full description.
Read the full description.

... topoisomerase with a novel mode of action. There are several advantages of topoisomerase inhibitors as antibiotics. Bacteria contain the two type IIA topoisomerases DNA gyrase (topoisomerase II) and topoisomerase IV. The active sites show a high degree of similarity, thus one antibiotic can potentia ...
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Chemosynthesis for the Classroom (6 pages, 464k)
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... upon sunlight as their primary source of energy. Instead, these communities derive their energy from chemicals through a process called chemosynthesis (in contrast to photosynthesis in which sunlight is the basic energy source). Some chemosynthetic communities have been found near underwater volcani ...
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... Bacterial Disease in Humans Growth of pathogenic bacteria disrupts the body’s equilibrium by interfering with its normal activities and producing disease. ...
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Question set no: Page no: 31 31 1. Name some chemical

... 3. Can you tell us any method of sterilization without heat? What are the mechanisms of action of those sterilization processes? ...
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... sulfur, these radioactive substances could be used as markers, enabling the scientists to tell which molecules actually entered the bacteria and carried the genetic information of the virus. ...
Prokaryotic Cells
Prokaryotic Cells

... diameter, associated with the outer membrane. Such discs have never been reported in peritrichously flagellated bacteria and their function is unknown (Coulton and Murray, 1977). Recent evidence indicates that current preparations do not remove all of the basal-body components. Recent preparations h ...
Characteristics of invasion of H Ep-2 cells by Providencia alcalifa ciens
Characteristics of invasion of H Ep-2 cells by Providencia alcalifa ciens

... Quantitative assay. The procedure for determining HEp-2 cell invasion has been described previously.2 HEp-2 cells were seeded on glass coverslips (Bellco Glass Inc., Vineland, NJ, USA) placed in the bottom of 1-dram (3.7-ml) glass shell vials (American Scientific Products, McGraw Park, IL, USA) and ...
trisan - Arcochimica
trisan - Arcochimica

... Hazard labelling pursuant to EC Regulation 1272/2008 (CLP) and subsequent amendments and supplements. Hazard statements: Flammable liquid and vapour. May cause an allergic skin reaction. Harmful to aquatic life with long lasting effects. Contains: 1,5-pentanedial , Poly(hexamethylene) biguanide hydr ...
Coordination of Multiple Flagella in Bacteria - q
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... affects the downstream response of multiple flagellar motors by constructing coarse-grained models of the signaling noise, the bacterial flagellar motor, and the interactions of multiple flagella. Interestingly, we find that cells can exploit the upstream signaling noise to coordinate the response o ...
2_Chisto+Gille+Shiel+Tayl+Tlus_Baird2010
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... Bacteria of healthy vs lesioned shell • Genetic signatures of bacteria on shell span multiple major taxonomic groups, potentially comprised of 100’s of species ...
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A1980KF12400001

... the University of Pennsylvania. Our other coworkers have raised families and have then returned to the laboratory. “Although it is now some 20 years since the discovery that fluorouracil provokes ‘thymineless death,’ the nature of these events is less than crystal clear. Most workers today do believ ...
phenotypic
phenotypic

... • Enzyme-mediated metabolic reactions often visualized by a color change - microbe is cultured in a medium with a special substrate, then tested for a particular end product - microbial expression of the enzyme is made visible by a colored dye ...
Gram staining
Gram staining

... polysaccharides) that protect the cell. Usually negative staining is used (capsule is an unstained place on a stained background) • Biofilm is a complex layer, composed of bacteria, their capsullae and other material. Biofilm is much stronger than individual bacteria, living in so named planctonic f ...
Microbes In Human Welfaregps
Microbes In Human Welfaregps

... 1. Biological control is defined as the reduction of pest populations by natural enemies and typically involves an active human role. ...
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Bacteria



Bacteria (/bækˈtɪəriə/; singular: bacterium) constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria have a number of shapes, ranging from spheres to rods and spirals. Bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep portions of Earth's crust. Bacteria also live in symbiotic and parasitic relationships with plants and animals. They are also known to have flourished in manned spacecraft.There are typically 40 million bacterial cells in a gram of soil and a million bacterial cells in a millilitre of fresh water. There are approximately 5×1030 bacteria on Earth, forming a biomass which exceeds that of all plants and animals. Bacteria are vital in recycling nutrients, with many of the stages in nutrient cycles dependent on these organisms, such as the fixation of nitrogen from the atmosphere and putrefaction. In the biological communities surrounding hydrothermal vents and cold seeps, bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. On 17 March 2013, researchers reported data that suggested bacterial life forms thrive in the Mariana Trench, which with a depth of up to 11 kilometres is the deepest part of the Earth's oceans. Other researchers reported related studies that microbes thrive inside rocks up to 580 metres below the sea floor under 2.6 kilometres of ocean off the coast of the northwestern United States. According to one of the researchers, ""You can find microbes everywhere — they're extremely adaptable to conditions, and survive wherever they are.""Most bacteria have not been characterized, and only about half of the phyla of bacteria have species that can be grown in the laboratory. The study of bacteria is known as bacteriology, a branch of microbiology.There are approximately ten times as many bacterial cells in the human flora as there are human cells in the body, with the largest number of the human flora being in the gut flora, and a large number on the skin. The vast majority of the bacteria in the body are rendered harmless by the protective effects of the immune system, and some are beneficial. However, several species of bacteria are pathogenic and cause infectious diseases, including cholera, syphilis, anthrax, leprosy, and bubonic plague. The most common fatal bacterial diseases are respiratory infections, with tuberculosis alone killing about 2 million people per year, mostly in sub-Saharan Africa. In developed countries, antibiotics are used to treat bacterial infections and are also used in farming, making antibiotic resistance a growing problem. In industry, bacteria are important in sewage treatment and the breakdown of oil spills, the production of cheese and yogurt through fermentation, and the recovery of gold, palladium, copper and other metals in the mining sector, as well as in biotechnology, and the manufacture of antibiotics and other chemicals.Once regarded as plants constituting the class Schizomycetes, bacteria are now classified as prokaryotes. Unlike cells of animals and other eukaryotes, bacterial cells do not contain a nucleus and rarely harbour membrane-bound organelles. Although the term bacteria traditionally included all prokaryotes, the scientific classification changed after the discovery in the 1990s that prokaryotes consist of two very different groups of organisms that evolved from an ancient common ancestor. These evolutionary domains are called Bacteria and Archaea.
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