Module 6 : Hypersensitivity and immunodeficiency
... widely spread in the body. Serum sickness, Arthus reaction, polyarteritis nodosa, and poststreptococcal glomerulonephritis are some of the immune complex mediated diseases. Serum sickness is an immunization of any individual with a non-self protein or foreign protein that leads to immune reaction. A ...
... widely spread in the body. Serum sickness, Arthus reaction, polyarteritis nodosa, and poststreptococcal glomerulonephritis are some of the immune complex mediated diseases. Serum sickness is an immunization of any individual with a non-self protein or foreign protein that leads to immune reaction. A ...
lec#5 done by Haneen Al-Khateeb
... large lymphocyte containing granules, direct contact btw NK cells & coated cell will kill the coated cell directly. 4-Ab transport through epithelium or placenta 5-Activation of mast cells, eosinophils and basophiles by IgE 6-neutralization: when we have a virus, the antibodies will coat the virus , ...
... large lymphocyte containing granules, direct contact btw NK cells & coated cell will kill the coated cell directly. 4-Ab transport through epithelium or placenta 5-Activation of mast cells, eosinophils and basophiles by IgE 6-neutralization: when we have a virus, the antibodies will coat the virus , ...
Table of contents
... 1. Overview Hematopoiesis is the process by which all the different cell lineages that form the blood and immune system are generated from a common pluripotent stem cell. During the life of an individual, two separate hematopoietic systems exist, both arising during embryonic development but only o ...
... 1. Overview Hematopoiesis is the process by which all the different cell lineages that form the blood and immune system are generated from a common pluripotent stem cell. During the life of an individual, two separate hematopoietic systems exist, both arising during embryonic development but only o ...
Sequential follow up observations of a patient with rubella
... Peptide coated microplate wells were subsequently exposed to 1:50 dilutions of patient or reference (15 IU/ml) or positive and negative control serum samples and incubated for 30 minutes at room temperature. After washing the plates with PBST, bound specific antibodies were detected by the sequentia ...
... Peptide coated microplate wells were subsequently exposed to 1:50 dilutions of patient or reference (15 IU/ml) or positive and negative control serum samples and incubated for 30 minutes at room temperature. After washing the plates with PBST, bound specific antibodies were detected by the sequentia ...
Chapter 20-22 Lymphatic System
... c.) signs – objective abnormalities that can be seen or measured by someone other than the patient d.) symptom – subjective abnormality felt only by the patient e.) syndrome – collection of different signs & symptoms, usually with a common cause, that presents a clear picture of a pathological condi ...
... c.) signs – objective abnormalities that can be seen or measured by someone other than the patient d.) symptom – subjective abnormality felt only by the patient e.) syndrome – collection of different signs & symptoms, usually with a common cause, that presents a clear picture of a pathological condi ...
169) `Immune Epitope Database (IEDB) and its use of formal
... T cells recognize epitopes (typically peptides) presented by MHC Molecules ...
... T cells recognize epitopes (typically peptides) presented by MHC Molecules ...
A role for complement and immune complexes in immune
... Pre-existing antibodies to murine IgG (relevant for chimeric antibodies) (known as false-positive reactions in e.g. ELISAs) Prevalence 1-80% (!) Two different groups of naturally occurring heterophilic antibodies (IgGtype) (Hennig et al., J Immunol Methods. 2000 Feb 21;235(1-2):71-80): Against the ...
... Pre-existing antibodies to murine IgG (relevant for chimeric antibodies) (known as false-positive reactions in e.g. ELISAs) Prevalence 1-80% (!) Two different groups of naturally occurring heterophilic antibodies (IgGtype) (Hennig et al., J Immunol Methods. 2000 Feb 21;235(1-2):71-80): Against the ...
Advanced Pharmacology-I (PHR5001) Lecture 12: Anti
... Monoclonal antibodies are widely diagnostic and research reagents. ...
... Monoclonal antibodies are widely diagnostic and research reagents. ...
B cell
... Antigenicity 抗原性: the ability to combine specifically with Ab and/or cell-surface receptors (Ig/TCR) ...
... Antigenicity 抗原性: the ability to combine specifically with Ab and/or cell-surface receptors (Ig/TCR) ...
Delivery of Antibodies to the Gastrointestinal Tract
... The optimum matrix type sustained release tablet was then coated with different combinations and thicknesses (as weight gain) of Eudragits L30D-55 and NE30D to achieve different delayed release times. The three tablets with each has different delayed release times were then incorporated in a size 00 ...
... The optimum matrix type sustained release tablet was then coated with different combinations and thicknesses (as weight gain) of Eudragits L30D-55 and NE30D to achieve different delayed release times. The three tablets with each has different delayed release times were then incorporated in a size 00 ...
Vaksin dan sera
... is inserted into a plasmid. This genetically engineered plasmid comprises the DNA vaccine which is then injected into the host. Within the host cells, the foreign gene can be expressed (transcribed and translated) from the plasmid DNA, and if sufficient amounts of the foreign protein are produced, t ...
... is inserted into a plasmid. This genetically engineered plasmid comprises the DNA vaccine which is then injected into the host. Within the host cells, the foreign gene can be expressed (transcribed and translated) from the plasmid DNA, and if sufficient amounts of the foreign protein are produced, t ...
FOOD ALLERGEN TESTING
... • While kits all appear similar, there seems to be considerable variation in the degree of development and validation carried out • Peanut kits from major manufacturers are generally the best and have been through AOAC method assessment process • Some kits are perfectly satisfactory for screening, b ...
... • While kits all appear similar, there seems to be considerable variation in the degree of development and validation carried out • Peanut kits from major manufacturers are generally the best and have been through AOAC method assessment process • Some kits are perfectly satisfactory for screening, b ...
Polyclonal Antibody Production Guidelines Introduction Since the
... several minutes. An emulsion is properly prepared when it becomes thick, is difficult to inject back and forth through the cannula, and will not separate on standing; a droplet placed into a saline solution should not disperse. Emulsification is enhanced by using cold (4°C) adjuvant. Failure of the ...
... several minutes. An emulsion is properly prepared when it becomes thick, is difficult to inject back and forth through the cannula, and will not separate on standing; a droplet placed into a saline solution should not disperse. Emulsification is enhanced by using cold (4°C) adjuvant. Failure of the ...
FOOD ALLERGEN TESTING
... • While kits all appear similar, there seems to be considerable variation in the degree of development and validation carried out • Peanut kits from major manufacturers are generally the best and have been through AOAC method assessment process • Some kits are perfectly satisfactory for screening, b ...
... • While kits all appear similar, there seems to be considerable variation in the degree of development and validation carried out • Peanut kits from major manufacturers are generally the best and have been through AOAC method assessment process • Some kits are perfectly satisfactory for screening, b ...
File
... Active immunity is where the body cells are stimulated so that they make their own antibodies. The vaccine may cause some of the milder symptoms of the disease to appear, but the person will be protected from a serious attack. Passive immunity, which does not last as long as active immunity, is ach ...
... Active immunity is where the body cells are stimulated so that they make their own antibodies. The vaccine may cause some of the milder symptoms of the disease to appear, but the person will be protected from a serious attack. Passive immunity, which does not last as long as active immunity, is ach ...
Immunity Answers
... Describe phagocytosis by a neutrophil using the words: histamine, antibody, engulf and lysosome. Cells which are infected by pathogens releases histamine which attracts neutrophils. Pathogens are then engulfed by the neutrophils. Antibodies may have become attached to the antigens on the surface o ...
... Describe phagocytosis by a neutrophil using the words: histamine, antibody, engulf and lysosome. Cells which are infected by pathogens releases histamine which attracts neutrophils. Pathogens are then engulfed by the neutrophils. Antibodies may have become attached to the antigens on the surface o ...
CRP (C-Reactive Protein)
... Carried by white blood cells into the liver, spleen, and bone marrow Multiply and reenter the bloodstream (Clinical illness) Bacteria invade the gallbladder, biliary system, and the lymphatic tissue of the bowel and multiply in high numbers Then pass into the intestinal tract (can be identified for ...
... Carried by white blood cells into the liver, spleen, and bone marrow Multiply and reenter the bloodstream (Clinical illness) Bacteria invade the gallbladder, biliary system, and the lymphatic tissue of the bowel and multiply in high numbers Then pass into the intestinal tract (can be identified for ...
No Slide Title
... Approx. 140 genes, ~70 of these are involved in immune responses. • HLA: Human Leukocyte Antigens. Is the name of the MHC in humans = interchangeable. ...
... Approx. 140 genes, ~70 of these are involved in immune responses. • HLA: Human Leukocyte Antigens. Is the name of the MHC in humans = interchangeable. ...
Membrane Receptors for Antigen
... • Expressed on nearly all cells of the animal, though are more abundant on some cells than others • In humans there are three main genes, located on Chromosome 6, called HLA-A, HLA-B and HLA-C (HLA stands for Human Leukocyte Antigen – they were originally called antigens because animals make antibod ...
... • Expressed on nearly all cells of the animal, though are more abundant on some cells than others • In humans there are three main genes, located on Chromosome 6, called HLA-A, HLA-B and HLA-C (HLA stands for Human Leukocyte Antigen – they were originally called antigens because animals make antibod ...
Immunity_Hypersensitivity_Allergy_and_Autoimmune_Diseases
... organism or its products, usually possesses some degree of immunity as well • Many diseases are associated with the development of an acquired immunity without demonstrable hypersensitivity • Normally, a person develops an immune response only against foreign antigens (non-self antigens) because the ...
... organism or its products, usually possesses some degree of immunity as well • Many diseases are associated with the development of an acquired immunity without demonstrable hypersensitivity • Normally, a person develops an immune response only against foreign antigens (non-self antigens) because the ...
notes
... • When an antigen enters the body, the B cells that best bind with the antigens proliferate by cloning. The B cells clone a specific type of antibody • The strength of binding is dependent on how well (closely) the paratope on the antibody bind with the epitope of the antigen. This property is calle ...
... • When an antigen enters the body, the B cells that best bind with the antigens proliferate by cloning. The B cells clone a specific type of antibody • The strength of binding is dependent on how well (closely) the paratope on the antibody bind with the epitope of the antigen. This property is calle ...
Cellular Apoptosis Susceptibility Protein Data Sheet
... Cellular apoptosis susceptibility protein, the product of the CAS gene, is associated with microtubules and the mitotic spindle. CAS is the human homolog of the yeast chromosomesegregation gene, CSE-1. The molecular mechanism or function by which CAS is associated with cell proliferation and apoptos ...
... Cellular apoptosis susceptibility protein, the product of the CAS gene, is associated with microtubules and the mitotic spindle. CAS is the human homolog of the yeast chromosomesegregation gene, CSE-1. The molecular mechanism or function by which CAS is associated with cell proliferation and apoptos ...
monitoring compliance with treatment
... • Screening data is “accepted” into the LIMS system and that triggers the order in the LIMS system for confirmations. • The confirm ordered samples are retrieved and a portion of it is placed into a test tube. • That sample then undergoes a series of sample preparation steps before being placed onto ...
... • Screening data is “accepted” into the LIMS system and that triggers the order in the LIMS system for confirmations. • The confirm ordered samples are retrieved and a portion of it is placed into a test tube. • That sample then undergoes a series of sample preparation steps before being placed onto ...
The immune system - Los Angeles Mission College
... Phagocytes include eosinophil (which only phagocytize antigens in the blood), neutrophil (which can phagocytize small antigens in connective tissues, out of the blood ), and monocyte which develops into macrophage (which can phagocytize larger antigens in connective tissues). ...
... Phagocytes include eosinophil (which only phagocytize antigens in the blood), neutrophil (which can phagocytize small antigens in connective tissues, out of the blood ), and monocyte which develops into macrophage (which can phagocytize larger antigens in connective tissues). ...
ELISA
The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance.ELISA is a popular format of ""wet-lab"" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a ""sandwich"" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Of note, ELISA can perform other forms of ligand binding assays instead of strictly ""immuno"" assays, though the name carried the original ""immuno"" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or ""immunosorbed"" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, and so are not easily reusable.