The Maternal Gene skn.1 Encodes a Protein That Is Distributed
... cleavage rate, and mitotic spindle orientation. Reproducible differences in the fates of these blastomeres were first described almost 100 years ago. For example, limited cell lineage studies suggested that only the posterior blastomere produces intestinal cells in the nematode Parascaris (Boveri, 1 ...
... cleavage rate, and mitotic spindle orientation. Reproducible differences in the fates of these blastomeres were first described almost 100 years ago. For example, limited cell lineage studies suggested that only the posterior blastomere produces intestinal cells in the nematode Parascaris (Boveri, 1 ...
Protocol for Real-Time RT-PCR - MGH-PGA
... Some genes are expressed transiently or only in certain tissues. In our experience, this is the most likely cause for negative PCR results. Please read literature for the gene expression patterns. One caveat is that microarrays are not always reliable at measuring gene expressions. After switching t ...
... Some genes are expressed transiently or only in certain tissues. In our experience, this is the most likely cause for negative PCR results. Please read literature for the gene expression patterns. One caveat is that microarrays are not always reliable at measuring gene expressions. After switching t ...
Ectopic segmentation gene expression and
... alternate metameres. Nevertheless, the double-metameric primordia must regulate to give approximately normal-sized cuticular segments. It has not yet proved possible to follow the fates of individual cells during this process. If size regulation is due to an intrinsic response of cells to mutant pat ...
... alternate metameres. Nevertheless, the double-metameric primordia must regulate to give approximately normal-sized cuticular segments. It has not yet proved possible to follow the fates of individual cells during this process. If size regulation is due to an intrinsic response of cells to mutant pat ...
Balanced reciprocal translocation at amniocentesis - FUNPEC-RP
... death, and the transmission of chromosomal abnormalities to their offspring through the production of a higher number of unbalanced spermatozoa (Godo et al., 2013). Because the carriers of balanced translocations have an increased risk of pregnancy loss, fetal death, and the transmission of chromoso ...
... death, and the transmission of chromosomal abnormalities to their offspring through the production of a higher number of unbalanced spermatozoa (Godo et al., 2013). Because the carriers of balanced translocations have an increased risk of pregnancy loss, fetal death, and the transmission of chromoso ...
Genetic and experimental studies on a pigment
... these conditions, it was also clear that P^/P^ xanthophores and iridophores were less intensely pigmented than their wild-type neighbors. Ultrastructural studies will be necessary to further clarify the nature of these differences. Bagnara et al. (1979) have suggested that melanophores, xanthophores ...
... these conditions, it was also clear that P^/P^ xanthophores and iridophores were less intensely pigmented than their wild-type neighbors. Ultrastructural studies will be necessary to further clarify the nature of these differences. Bagnara et al. (1979) have suggested that melanophores, xanthophores ...
Production of loach (Misgurnus anguillicaudatus) germ
... Isolated blastomere suspension (in sodium citrate) was diluted 4-fold in a cryosolution (1 part blastomere suspension to 3 parts cryosolution) and mixed. This procedure resulted in a cell concentration of 356 × 103 ± 75 cells/mL. The cryosolution was prepared with Eagle’s minimum essential medium (M ...
... Isolated blastomere suspension (in sodium citrate) was diluted 4-fold in a cryosolution (1 part blastomere suspension to 3 parts cryosolution) and mixed. This procedure resulted in a cell concentration of 356 × 103 ± 75 cells/mL. The cryosolution was prepared with Eagle’s minimum essential medium (M ...
Birefringence imaging of spermatozoa, spindle and zona pellucida
... • Use of acrosome acrosome-reacted reacted spermatozoa (identified by polarization microscopy) raises implantation and p pregnancy g y rates for severe Oat and testicular spermatozoa ...
... • Use of acrosome acrosome-reacted reacted spermatozoa (identified by polarization microscopy) raises implantation and p pregnancy g y rates for severe Oat and testicular spermatozoa ...
File
... Bob and Carol know that there is a risk that their next baby will have cystic fibrosis. Embryos can be screened for the allele that produces cystic fibrosis. Many people support the screening of embryos, but others do not. (i) ...
... Bob and Carol know that there is a risk that their next baby will have cystic fibrosis. Embryos can be screened for the allele that produces cystic fibrosis. Many people support the screening of embryos, but others do not. (i) ...
View PDF - OMICS International
... According to DNA polymorphism studies performed in families with aneuploid spontaneous abortions and live-born babies, chromosomal abnormalities originate predominantly from female meiosis I, probably due to reduction of meiotic recombination rate with advanced reproductive age [1-3]. It was also su ...
... According to DNA polymorphism studies performed in families with aneuploid spontaneous abortions and live-born babies, chromosomal abnormalities originate predominantly from female meiosis I, probably due to reduction of meiotic recombination rate with advanced reproductive age [1-3]. It was also su ...
Q-RT-PCR replaced 4-21-06
... d. Initial evaluation should also include NRTC (no reverse transcriptase control) e. Standard curves can be generated using serial dilutions of cDNA with highest content of mRNA of interest 8. Cap tubes and place in PE 5700 QPCR machine 9. Run at 950C for 10 minutes and then 40 cycles of 950C for 15 ...
... d. Initial evaluation should also include NRTC (no reverse transcriptase control) e. Standard curves can be generated using serial dilutions of cDNA with highest content of mRNA of interest 8. Cap tubes and place in PE 5700 QPCR machine 9. Run at 950C for 10 minutes and then 40 cycles of 950C for 15 ...
Leapfrogging: primordial germ cell transplantation
... (e.g. Fig. 2C). Three of the four remaining test crosses resulted in no albino embryos whereas the fourth had 41% transmission of mutant alleles. There are several possible explanations for the cases where low tyr transmission rates were observed. First, mutagenesis in some F0 donor embryos might ha ...
... (e.g. Fig. 2C). Three of the four remaining test crosses resulted in no albino embryos whereas the fourth had 41% transmission of mutant alleles. There are several possible explanations for the cases where low tyr transmission rates were observed. First, mutagenesis in some F0 donor embryos might ha ...
Unbalanced Translocation Breakout
... • Adoption • Prenatal Testing • Chorionic villus sampling (CVS) – at about 11 to 12 weeks • Amniocentesis – at about 16 to 18 weeks of pregnancy • Non-Invasive Prenatal Testing (NIPT) analyzes cellfree fetal DNA circulating in maternal blood – at about 10 to 22 weeks ...
... • Adoption • Prenatal Testing • Chorionic villus sampling (CVS) – at about 11 to 12 weeks • Amniocentesis – at about 16 to 18 weeks of pregnancy • Non-Invasive Prenatal Testing (NIPT) analyzes cellfree fetal DNA circulating in maternal blood – at about 10 to 22 weeks ...
209 Original Scientific Article THE INFLUENCE OF
... One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is already known that the epigenetic enzymes transcription in ooc ...
... One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is already known that the epigenetic enzymes transcription in ooc ...
Detection of Large Expansions in SCA8 Using a Fluorescent Repeat
... Spinocerebellar ataxia type 8 (SCA8) is a neurodegenerative disorder characterized by slowly progressive cerebellar ataxia. Koob et al first reported this neurodegenerative disorder as autosomal dominant spinocerebellar ataxia in a large kindred6). It is caused by bidirectional expression of (CUG)n ...
... Spinocerebellar ataxia type 8 (SCA8) is a neurodegenerative disorder characterized by slowly progressive cerebellar ataxia. Koob et al first reported this neurodegenerative disorder as autosomal dominant spinocerebellar ataxia in a large kindred6). It is caused by bidirectional expression of (CUG)n ...
1 Oviduct-embryo interactions in cattle
... kg weight) were managed and synchronized as described for Experiment 1. In order to ensure that ovulation had occurred at the time of embryo transfer, 0.02 mg of a GnRH agonist (buserelin) was administered 32 h after CIDR removal. Heifers were observed for signs of estrus four times per day commenci ...
... kg weight) were managed and synchronized as described for Experiment 1. In order to ensure that ovulation had occurred at the time of embryo transfer, 0.02 mg of a GnRH agonist (buserelin) was administered 32 h after CIDR removal. Heifers were observed for signs of estrus four times per day commenci ...
Zygotic genes that mediate torso receptor tyrosine kinase
... developmental pathway in the central region of the embryo. We have extended this genetic interaction screen by testing an additional 25 loci for suppression or enhancement of the central segment-loss phenotype of tor gain-of-function alleles (Table 1). Although mutations in these loci have been desc ...
... developmental pathway in the central region of the embryo. We have extended this genetic interaction screen by testing an additional 25 loci for suppression or enhancement of the central segment-loss phenotype of tor gain-of-function alleles (Table 1). Although mutations in these loci have been desc ...
Argonaute2 Is Essential for Mammalian
... (Figure S1B and S1C), which may account for the ability of some Ago2 mutants to escape gastrulation arrest and develop until midgestation [11]. Also consistent with previous studies is the reduced extraembryonic region in the e7.5 Ago2 mutant embryos; this finding further suggests embryos that survi ...
... (Figure S1B and S1C), which may account for the ability of some Ago2 mutants to escape gastrulation arrest and develop until midgestation [11]. Also consistent with previous studies is the reduced extraembryonic region in the e7.5 Ago2 mutant embryos; this finding further suggests embryos that survi ...
Methylation Dynamics in the Early Mammalian Embryo - Beck-Shop
... both sister chromatids and spatially separated from the fully demethylated paternal chromosomes. B Two-cell embryo in which one cell goes through second mitosis. The interphase nucleus exhibits a methylated maternal and a demethylated paternal compartment. During second mitosis, only one of the two ...
... both sister chromatids and spatially separated from the fully demethylated paternal chromosomes. B Two-cell embryo in which one cell goes through second mitosis. The interphase nucleus exhibits a methylated maternal and a demethylated paternal compartment. During second mitosis, only one of the two ...
Methylation Dynamics in the Early Mammalian Embryo: Implications
... both sister chromatids and spatially separated from the fully demethylated paternal chromosomes. B Two-cell embryo in which one cell goes through second mitosis. The interphase nucleus exhibits a methylated maternal and a demethylated paternal compartment. During second mitosis, only one of the two ...
... both sister chromatids and spatially separated from the fully demethylated paternal chromosomes. B Two-cell embryo in which one cell goes through second mitosis. The interphase nucleus exhibits a methylated maternal and a demethylated paternal compartment. During second mitosis, only one of the two ...
Warszawa, dnia 7 stycznia 2002 r
... describe screening tests carried out to identify from among the general population of apparently healthy individuals, those at risk of transmitting a genetic disorder to their offspring. Prenatal genetic screening may take place during pregnancy and involves testing people of either sex. The Princip ...
... describe screening tests carried out to identify from among the general population of apparently healthy individuals, those at risk of transmitting a genetic disorder to their offspring. Prenatal genetic screening may take place during pregnancy and involves testing people of either sex. The Princip ...
- Annals of Forest Science
... Rutaceae) are concerned [20, 27]. It has never been observed in in planta reproduction of gymnosperms [24] although some references report apomictic type deviations from the sexual cycle of conifers [4, 11, 23]. In comparison with “maternal apomixis”, C. dupreziana embryo development from paternal t ...
... Rutaceae) are concerned [20, 27]. It has never been observed in in planta reproduction of gymnosperms [24] although some references report apomictic type deviations from the sexual cycle of conifers [4, 11, 23]. In comparison with “maternal apomixis”, C. dupreziana embryo development from paternal t ...
Lecture 11-POSTED-BISC441-2012
... (2) A potential conflict is expected over maintenance of pregnancy: ->fetus selected for phenotypes (and underlying alleles) that maintain pregnancy, ->mother is selected to (a) abort any fetus in poor circumstances, (b) screen fetuses for quality (lack of genetically-based defects) Especially stron ...
... (2) A potential conflict is expected over maintenance of pregnancy: ->fetus selected for phenotypes (and underlying alleles) that maintain pregnancy, ->mother is selected to (a) abort any fetus in poor circumstances, (b) screen fetuses for quality (lack of genetically-based defects) Especially stron ...
Rapid and Quantitative Detection of Toxoplasma Gondii by PCR
... brew” applications for prenatal diagnosis of toxoplasmosis, in particular, making it faster, more sensitive, and decreases morbidity, because it is currently based on amniocentesis alone [4]. However, the main risk concerns false-positive results arising from contamination with previously amplified ...
... brew” applications for prenatal diagnosis of toxoplasmosis, in particular, making it faster, more sensitive, and decreases morbidity, because it is currently based on amniocentesis alone [4]. However, the main risk concerns false-positive results arising from contamination with previously amplified ...