Manufacturing Recombinant Protein Therapeutics under Cost
... in downstream processing (DSP). A first aspect impacting the manufacturing costs is the protein structure. Dr. Schmidt presented an overview of the DSP step sequences for a number of different complex proteins such as mAbs, Fc-fusion proteins (FcF), protein vaccines (Vac), enzymes (Enz) and fusion p ...
... in downstream processing (DSP). A first aspect impacting the manufacturing costs is the protein structure. Dr. Schmidt presented an overview of the DSP step sequences for a number of different complex proteins such as mAbs, Fc-fusion proteins (FcF), protein vaccines (Vac), enzymes (Enz) and fusion p ...
RISE-Workshop
... – A much greater range of protein Mr values can be separated than on a fixed percentage gels. In a complex mixture, very low-mol-wt proteins travel freely through the gel to begin with, and start to resolve when they reach the smaller pore size toward the lower part of the gel. Much larger proteins ...
... – A much greater range of protein Mr values can be separated than on a fixed percentage gels. In a complex mixture, very low-mol-wt proteins travel freely through the gel to begin with, and start to resolve when they reach the smaller pore size toward the lower part of the gel. Much larger proteins ...
Protein
... How to Calculate Your Protein Needs: 1. Weight in pounds divided by 2.2 = weight in kg 2. Weight in kg x 0.8-1.8 gm/kg = protein gm. Use a lower number if you are in good health and are sedentary (i.e., 0.8). Use a higher number (between 1 and 1.8) if you are under stress, are pregnant, are r ...
... How to Calculate Your Protein Needs: 1. Weight in pounds divided by 2.2 = weight in kg 2. Weight in kg x 0.8-1.8 gm/kg = protein gm. Use a lower number if you are in good health and are sedentary (i.e., 0.8). Use a higher number (between 1 and 1.8) if you are under stress, are pregnant, are r ...
LC/MS Study of Casein Proteins in Milk
... different degrees. The alpha and beta caseins are insoluble in water and are bound together with the more soluble kappacasein to form micelles. The caseins are extracted from milk with a denaturing 8 M urea protocol, which breaks apart the micelles and solubilizes the proteins. Chromatography of the ...
... different degrees. The alpha and beta caseins are insoluble in water and are bound together with the more soluble kappacasein to form micelles. The caseins are extracted from milk with a denaturing 8 M urea protocol, which breaks apart the micelles and solubilizes the proteins. Chromatography of the ...
Posttranslational Modification
... The azide is distinguished from all cellular functionality by its unique chemical reactivity with phosphine probes, a reaction termed the Staudinger ligation. Thus, proteins modified with GalNAz, a marker of O-linked glycans, can be selectively tagged for visualization or enrichment ...
... The azide is distinguished from all cellular functionality by its unique chemical reactivity with phosphine probes, a reaction termed the Staudinger ligation. Thus, proteins modified with GalNAz, a marker of O-linked glycans, can be selectively tagged for visualization or enrichment ...
SilenciO - PROvendis
... Chinese Hamster ovary (CHO) cells are commonly used as a mammalian expression system for the production of recombinant proteins. They are one of the few cell types that can be grown at high cell density as the cells are resistant to metabolic stress. The SilenciO-technology is a powerful tool for th ...
... Chinese Hamster ovary (CHO) cells are commonly used as a mammalian expression system for the production of recombinant proteins. They are one of the few cell types that can be grown at high cell density as the cells are resistant to metabolic stress. The SilenciO-technology is a powerful tool for th ...
Protein 101
... •How does this fit with Rx for athletes •Upper end of range clearly exceeds Rx for “athlete” Rx •*1.2-1.4 g/d /kg for endurance athletes *1.4-1.8 g/d/kg for strength athletes are adequate to support the ...
... •How does this fit with Rx for athletes •Upper end of range clearly exceeds Rx for “athlete” Rx •*1.2-1.4 g/d /kg for endurance athletes *1.4-1.8 g/d/kg for strength athletes are adequate to support the ...
Bordetella avium is Gram negative bacterium found in the upper
... bordetellosis. There are many genetic similarities between Bordetella avium, and its ancestors Bordetella pertussis and Bordetella bronchiseptica, approximately 75% similar on the genetic level. Therefore, searching for similarities in autotransporters for vaccinations in the other two lineages may ...
... bordetellosis. There are many genetic similarities between Bordetella avium, and its ancestors Bordetella pertussis and Bordetella bronchiseptica, approximately 75% similar on the genetic level. Therefore, searching for similarities in autotransporters for vaccinations in the other two lineages may ...
Using Computers to teach Undergraduates about Biological Molecules
... random sequences of M, about 14OOO or can have specific sequences added to it. Primary structures may be completely determined by simulating traditional protein biochemical approaches. Students can normally determine the sequence of a polypeptide of 70-80 residues in about two hours. Reasonably real ...
... random sequences of M, about 14OOO or can have specific sequences added to it. Primary structures may be completely determined by simulating traditional protein biochemical approaches. Students can normally determine the sequence of a polypeptide of 70-80 residues in about two hours. Reasonably real ...
Proteins
... water and unaffected by moderate changes in temperature and pH. Subgroups within this category include: Collagens & Elastins, the proteins of connective tissues. tendons and ligaments. Keratins, proteins that are major components of skin, hair, feathers and horn. Fibrin, a protein formed when blood ...
... water and unaffected by moderate changes in temperature and pH. Subgroups within this category include: Collagens & Elastins, the proteins of connective tissues. tendons and ligaments. Keratins, proteins that are major components of skin, hair, feathers and horn. Fibrin, a protein formed when blood ...
Nickel-NTA-Nanogold Binds His
... by such techniques. For multi-subunit complexes, a particular subunit may be labeled to specifically identify it in the complex. In x-ray or electron protein crystallography, the label could be used to improve contrast or be used as a phasing aid. Another difference in this type of label is that the ...
... by such techniques. For multi-subunit complexes, a particular subunit may be labeled to specifically identify it in the complex. In x-ray or electron protein crystallography, the label could be used to improve contrast or be used as a phasing aid. Another difference in this type of label is that the ...
Introduction to Proteins
... is similar but includes a sulfur atom. Other amino acids have R groups that range from a single hydrogen atom up to a double ring of carbon and nitrogen atoms, as you can see in the next plate. Color titles B, D, and E, the arrows (B and D), the dipeptide, and the water molecule. Just as with carboh ...
... is similar but includes a sulfur atom. Other amino acids have R groups that range from a single hydrogen atom up to a double ring of carbon and nitrogen atoms, as you can see in the next plate. Color titles B, D, and E, the arrows (B and D), the dipeptide, and the water molecule. Just as with carboh ...
more details
... independently, although it is well appreciated that substitution rates in a protein are influenced by changes in the amino acids at other locations. Generating more accurate but computationally tractable models of the evolutionary process will require a better understanding of the properties of such ...
... independently, although it is well appreciated that substitution rates in a protein are influenced by changes in the amino acids at other locations. Generating more accurate but computationally tractable models of the evolutionary process will require a better understanding of the properties of such ...
1471-2164-9-462-S2
... a frame-shift mutation, has become a pseudogene. MUDP_038 comprises part of an 8.4kb region that has been deleted from both pMUM001 and pMUM002. In Streptomyces spp. the plasmid-encoded FtsK/SpoIIIE domain protein, TraB, is involved in plasmid transfer [12-14]. MUDP_038 has similarity to FtsK/SpoIII ...
... a frame-shift mutation, has become a pseudogene. MUDP_038 comprises part of an 8.4kb region that has been deleted from both pMUM001 and pMUM002. In Streptomyces spp. the plasmid-encoded FtsK/SpoIIIE domain protein, TraB, is involved in plasmid transfer [12-14]. MUDP_038 has similarity to FtsK/SpoIII ...
We propose a frequent pattern-based algorithm for predicting
... We propose a frequent pattern-based algorithm for predicting functions and localizations of proteins from their primary structure (amino acid sequence). We use reduced alphabets that capture the higher rate of substitution between amino acids that are physiochemically similar. Frequent sub strings a ...
... We propose a frequent pattern-based algorithm for predicting functions and localizations of proteins from their primary structure (amino acid sequence). We use reduced alphabets that capture the higher rate of substitution between amino acids that are physiochemically similar. Frequent sub strings a ...
Proteins
... Another important property is the distinction between hydrophobic and hydrophylic amino acids. Proteins tend to fold in such a way that hydrophylic amino acids will be exposed to aqueous environments, such as the cytoplasm, whereas hydrophobic amino acids tend be located away from water, for example ...
... Another important property is the distinction between hydrophobic and hydrophylic amino acids. Proteins tend to fold in such a way that hydrophylic amino acids will be exposed to aqueous environments, such as the cytoplasm, whereas hydrophobic amino acids tend be located away from water, for example ...
Functional Genomics and Proteomics, Institute of
... Analysis of Biologically Important Molecular Complexes • Telomere biology ...
... Analysis of Biologically Important Molecular Complexes • Telomere biology ...
Proteolysis in Mixed Organic-Aqueous Solvent
... 2682 Analytical Chemistry, Vol. 73, No. 11, June 1, 2001 ...
... 2682 Analytical Chemistry, Vol. 73, No. 11, June 1, 2001 ...
Module 5
... against databases of motifs and profiles, or indeed both. Some commonly used programmes are listed below: Pfam is a collection of multiple alignments and profile hidden Markov models of protein domain families, which is based on proteins from both SWISS-PROT and SP-TrEMBL. SMART (a Simple Modular Ar ...
... against databases of motifs and profiles, or indeed both. Some commonly used programmes are listed below: Pfam is a collection of multiple alignments and profile hidden Markov models of protein domain families, which is based on proteins from both SWISS-PROT and SP-TrEMBL. SMART (a Simple Modular Ar ...
The George Washington University School of Medicine and Health
... – Director of Genomics and Proteomics MS program – Co-Director of the Institute for Proteomics Technology and development ...
... – Director of Genomics and Proteomics MS program – Co-Director of the Institute for Proteomics Technology and development ...
MNV-VPg-eIF4G-paper.SuppInfo.v2 07/08/2015 A conserved
... L939A – 1.04, H918A – 1.145, K901M-E914R – 1.15, L897A – 0.84). Therefore the purified proteins were thawed and incubated with 1 µM MgCl2 and 387 U benzonase nuclease (Sigma) for 70-90 minutes (typically 1 U benzonase per 0.02 mg protein). The mixture was then subjected to size-exclusion chromatogra ...
... L939A – 1.04, H918A – 1.145, K901M-E914R – 1.15, L897A – 0.84). Therefore the purified proteins were thawed and incubated with 1 µM MgCl2 and 387 U benzonase nuclease (Sigma) for 70-90 minutes (typically 1 U benzonase per 0.02 mg protein). The mixture was then subjected to size-exclusion chromatogra ...
chemistry_and_proteins
... disease and most cancers arise from a complex interplay among multiple genes and between genes and factors in the environment. ...
... disease and most cancers arise from a complex interplay among multiple genes and between genes and factors in the environment. ...
RACK-1, a receptor for activated C kinase, links metabotropic
... Local protein synthesis is activated by glutamate in synaptoneurosomes (Weiler, Greenough PNAS, 90:7168, 1993). To search for transmitter receptor triggered mechanisms involved in translational control of dendritically localized mRNAs, we focussed on mRNPs that might be affected by second messenger ...
... Local protein synthesis is activated by glutamate in synaptoneurosomes (Weiler, Greenough PNAS, 90:7168, 1993). To search for transmitter receptor triggered mechanisms involved in translational control of dendritically localized mRNAs, we focussed on mRNPs that might be affected by second messenger ...
Gene Section AKAP9 (A kinase (PRKA) anchor protein (yotiao) 9)
... Note: Breakpoint in AKAP9-BRAF fusion is located within intron 8 of the gene. In this fusion, exons 1-8 of AKAP9 are fused with last 10 exons 9-18 of BRAF. In the fusion, AKAP9 lacks the centrosome binding domain and, as a result, the AKAP9-BRAF protein looses its cytoplasmic compartmentalization an ...
... Note: Breakpoint in AKAP9-BRAF fusion is located within intron 8 of the gene. In this fusion, exons 1-8 of AKAP9 are fused with last 10 exons 9-18 of BRAF. In the fusion, AKAP9 lacks the centrosome binding domain and, as a result, the AKAP9-BRAF protein looses its cytoplasmic compartmentalization an ...
Post-Translational Modifications of Proteins
... Post-Translational Modifications of Proteins: from discovery to functional analysis ...
... Post-Translational Modifications of Proteins: from discovery to functional analysis ...
Protein mass spectrometry
Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as ""top-down"" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called ""bottom-up"" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.