Mechanisms of Translocation of Legionella pneumophila Effectors
... fusions of various L. pneumophila effectors to the rapidly and tightly folding dihydrofolate reductase (DHFR) protein. Fusions to DHFR prevented the translocation of nearly all the effectors studied, including a 50 amino acid carboxy-terminal tag of an effector protein, suggesting that tightly folde ...
... fusions of various L. pneumophila effectors to the rapidly and tightly folding dihydrofolate reductase (DHFR) protein. Fusions to DHFR prevented the translocation of nearly all the effectors studied, including a 50 amino acid carboxy-terminal tag of an effector protein, suggesting that tightly folde ...
Page 1 Proteins - Made up of amino acid monomers (yep, you got it
... common, but weak formed when δ+ H from –OH or –NH of the R group attract the δ- O of a –CO group, or another R group Ionic bonds: form between amino and carboyl groups on some R groups stronger than hydrogen bonds, but are weaker than disulphide bonds Disulphide bonds: covalent bond that is formed b ...
... common, but weak formed when δ+ H from –OH or –NH of the R group attract the δ- O of a –CO group, or another R group Ionic bonds: form between amino and carboyl groups on some R groups stronger than hydrogen bonds, but are weaker than disulphide bonds Disulphide bonds: covalent bond that is formed b ...
Protein Motif Analysis
... perform different sub-functions, and together these parts allow the entire protein to perform its overall function. These functionally distinct parts of the protein are known as functional domains. If they are conserved across taxa, these conserved domains can be identified by amino acid sequence si ...
... perform different sub-functions, and together these parts allow the entire protein to perform its overall function. These functionally distinct parts of the protein are known as functional domains. If they are conserved across taxa, these conserved domains can be identified by amino acid sequence si ...
GMP Recombinant Human Epidermal Growth Factor alpha (rh EGF)
... A DNA sequence encoding the mature EGF protein (Bell, G.I. et al, 1986, Nucl. Acids Res. 14: 8427) (including a C-terminal His6-tag) was expressed in E. coli ...
... A DNA sequence encoding the mature EGF protein (Bell, G.I. et al, 1986, Nucl. Acids Res. 14: 8427) (including a C-terminal His6-tag) was expressed in E. coli ...
Lecture 8
... After the initial fractionation steps we move to column chromatography. The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. This solution is passed through a column consisting of a porous solid matrix called the stationary phase. ...
... After the initial fractionation steps we move to column chromatography. The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. This solution is passed through a column consisting of a porous solid matrix called the stationary phase. ...
After oral administration, bioactive substances and drugs must be
... Enhancement of the water solubility of bioactive substances and drugs by complexation will increase their absorption efficiency and bioavailability. This will allow reduction of the dose required to achieve the desired response, and reduction of any harmful side effects of the drug. The formation of ...
... Enhancement of the water solubility of bioactive substances and drugs by complexation will increase their absorption efficiency and bioavailability. This will allow reduction of the dose required to achieve the desired response, and reduction of any harmful side effects of the drug. The formation of ...
Peptides and peptide bond
... α-carboxyl groups of the non-terminal amino acids are involved in the peptide bond so they cannot ionize nor contribute to the acid –base behavior of the peptide molecule. However the R-group when containing an ionizable group ( additional carboxyl or amino group) will contribute to the overall acid ...
... α-carboxyl groups of the non-terminal amino acids are involved in the peptide bond so they cannot ionize nor contribute to the acid –base behavior of the peptide molecule. However the R-group when containing an ionizable group ( additional carboxyl or amino group) will contribute to the overall acid ...
Protein-surface interactions: insights from atomistic - Cnr-Nano
... accidentally uptaken [1]; surgical implants) are readily covered with proteins, whose nature, orientation and subsequent conformational changes are determined by protein-inorganics interactions [2]. On the other hand, development of applications where proteins are used in nanotechnology contexts (e. ...
... accidentally uptaken [1]; surgical implants) are readily covered with proteins, whose nature, orientation and subsequent conformational changes are determined by protein-inorganics interactions [2]. On the other hand, development of applications where proteins are used in nanotechnology contexts (e. ...
Biotechnology Lab (Kallas)
... recovery, in vitro manipulation of genes, and data analysis that are fundamental to many areas of biotechnology. 2) To gain experience in critical thinking and experimental design to address interesting problems in biology or biotechnology. Topics include: analysis of DNA sequence databases, DNA amp ...
... recovery, in vitro manipulation of genes, and data analysis that are fundamental to many areas of biotechnology. 2) To gain experience in critical thinking and experimental design to address interesting problems in biology or biotechnology. Topics include: analysis of DNA sequence databases, DNA amp ...
File - Heritage FFA
... regulate body heat. Water also acts as a lubricant for the body's organs. Any living thing can live longer without food than without water. PROTEINS Proteins are complex chemical substances from which the body tissues are built. Each protein is comprised of smaller units called amino acids. Each spe ...
... regulate body heat. Water also acts as a lubricant for the body's organs. Any living thing can live longer without food than without water. PROTEINS Proteins are complex chemical substances from which the body tissues are built. Each protein is comprised of smaller units called amino acids. Each spe ...
protein expression after nacl treatment in two tomato cultivars
... immersed for 24 h in solutions containing 50, 100, 150, 200, 300 mM NaCl or water as control exhibited different responses. The first signs of wilting appeared at 100 mM NaCl in Castle rock and 200 mM NaCl in Edkawi (Fig. 1). The trend was similar for plants growing on MS-agar media at the same conc ...
... immersed for 24 h in solutions containing 50, 100, 150, 200, 300 mM NaCl or water as control exhibited different responses. The first signs of wilting appeared at 100 mM NaCl in Castle rock and 200 mM NaCl in Edkawi (Fig. 1). The trend was similar for plants growing on MS-agar media at the same conc ...
The use of isotope-coded affinity tags (ICAT)
... from the LeuD3 and Leu cells and their relative abundance can be calculated, enabling the quantitative comparison of protein levels in the two samples. Foster et al. [19] treated the Leu grown cells with a drug to disrupt lipid rafts. The drug-treated Leu cells were then pooled with untreated LeuD3 ...
... from the LeuD3 and Leu cells and their relative abundance can be calculated, enabling the quantitative comparison of protein levels in the two samples. Foster et al. [19] treated the Leu grown cells with a drug to disrupt lipid rafts. The drug-treated Leu cells were then pooled with untreated LeuD3 ...
Protein Ubiquitination
... *High proportion of proteins are translocated into the ER. *Proteins are translocated into the ER lumen in an unfolded state. *Inhibition of translation initiation serves as an effective means to limit the flow of proteins into the ER . ...
... *High proportion of proteins are translocated into the ER. *Proteins are translocated into the ER lumen in an unfolded state. *Inhibition of translation initiation serves as an effective means to limit the flow of proteins into the ER . ...
Confocal Fluorescence Microscopy
... or longer wavelengths (EYFP). Double-label studies using these different fluorescent proteins can be employed for fluorescent resonant energy transfer (FRET) studies. Table 2.3(b) summarizes the main spectral properties of the different fluorescent proteins. Figure 2.14 shows the excitation (i.e., the ...
... or longer wavelengths (EYFP). Double-label studies using these different fluorescent proteins can be employed for fluorescent resonant energy transfer (FRET) studies. Table 2.3(b) summarizes the main spectral properties of the different fluorescent proteins. Figure 2.14 shows the excitation (i.e., the ...
Direct Tissue Analysis by Matrix
... for direct tissue analysis depends on the molecular weight, hydrophobicity, and salt content of the analyte. Typically, 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid) is favorable for proteins (molecular weight ⬎ 2 kDa), and ␣-cyano-4-hydroxycinnamic acid (CHCA) is optimal for peptides (500-2 ...
... for direct tissue analysis depends on the molecular weight, hydrophobicity, and salt content of the analyte. Typically, 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid) is favorable for proteins (molecular weight ⬎ 2 kDa), and ␣-cyano-4-hydroxycinnamic acid (CHCA) is optimal for peptides (500-2 ...
Structure of Proteins
... This is again by hydrogen bonding. These bonds are WEAK bonds. -sheets can either be PARALLEL (where the adjacent polypeptide strands extend in the same direction) or ANTIPARALLEL (the strands extend in ...
... This is again by hydrogen bonding. These bonds are WEAK bonds. -sheets can either be PARALLEL (where the adjacent polypeptide strands extend in the same direction) or ANTIPARALLEL (the strands extend in ...
10-30-ramnath
... A common experimental procedure is to induce random mutations in the "wild-type" strain of a model organism (e.g., saccharomyces cerevisiae) and then screen the mutants for interesting observable characteristics (i.e. phenotype). Often the phenotype shows slower growth rates under certain conditions ...
... A common experimental procedure is to induce random mutations in the "wild-type" strain of a model organism (e.g., saccharomyces cerevisiae) and then screen the mutants for interesting observable characteristics (i.e. phenotype). Often the phenotype shows slower growth rates under certain conditions ...
Four immunosuppressive agents
... The liquid is introduced into the source using a thin capillary tube and is subjected to a strong electromagnetic field. Under the effect of a gas nebuliser, the liquid is transformed into a mist of fine highly charged droplets. The molecular ions or 'ion parents' formed then pass through the first ...
... The liquid is introduced into the source using a thin capillary tube and is subjected to a strong electromagnetic field. Under the effect of a gas nebuliser, the liquid is transformed into a mist of fine highly charged droplets. The molecular ions or 'ion parents' formed then pass through the first ...
Protein Structure - Particle Sciences
... analyzed by means of peptide mapping and the use of Edman degradation or mass spectroscopy. This process is routine for peptides and small proteins, but becomes more complex for large multimeric proteins. Peptide mapping generally entails treatment of the protein with different protease enzymes in o ...
... analyzed by means of peptide mapping and the use of Edman degradation or mass spectroscopy. This process is routine for peptides and small proteins, but becomes more complex for large multimeric proteins. Peptide mapping generally entails treatment of the protein with different protease enzymes in o ...
Protein Creation Pathway Tutorial
... Directions: Download and then open the PowerPoint from my website. Press the F5 button to start the presentation. ...
... Directions: Download and then open the PowerPoint from my website. Press the F5 button to start the presentation. ...
ab initio
... propensity of a position is calculated using an average over 5 or 6 residues surrounding each position. On a larger set of 62 proteins the base method reports a success rate of 50%. (page 446) •1978 Garnier improved the method by using statistically significant pair-wise interactions as a determinan ...
... propensity of a position is calculated using an average over 5 or 6 residues surrounding each position. On a larger set of 62 proteins the base method reports a success rate of 50%. (page 446) •1978 Garnier improved the method by using statistically significant pair-wise interactions as a determinan ...
The Amino Acid Sequence of Chlorella fusca Plastocyanin
... group. Nevertheless N-terminal group analysis by the dansyl method showed both alanine and serine. After removal of the haem (Hg2Cl2-0.l M-HC1-8M-urea, 3 7 T , 24h), the apoprotein was digested with trypsin or chymotrypsin, and the peptides formed were isolated and characterized by standard methods ...
... group. Nevertheless N-terminal group analysis by the dansyl method showed both alanine and serine. After removal of the haem (Hg2Cl2-0.l M-HC1-8M-urea, 3 7 T , 24h), the apoprotein was digested with trypsin or chymotrypsin, and the peptides formed were isolated and characterized by standard methods ...
Chapter 3
... from an unfolded state to their folded native state. This proves that the amino acid sequence contains enough information to specify tertiary structure. Bonds within the peptide backbone seek out different possible conformations as the final tertiary structure is achieved (Fig. 3.14). Folding tends ...
... from an unfolded state to their folded native state. This proves that the amino acid sequence contains enough information to specify tertiary structure. Bonds within the peptide backbone seek out different possible conformations as the final tertiary structure is achieved (Fig. 3.14). Folding tends ...
Protein mass spectrometry
Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as ""top-down"" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called ""bottom-up"" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.