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Ketone Bodies, Potential Therapeutic Uses
Ketone Bodies, Potential Therapeutic Uses

... of energy for brain per unit oxygen, supported by the same phenomenon noted in the isolated working perfused rat heart and in sperm. It has also been shown to decrease cell death in two human neuronal cultures, one a model of Alzheimer’s and the other of Parkinson’s disease. These observations raise ...
Dynamic Energy Budget theory - Vrije Universiteit Amsterdam
Dynamic Energy Budget theory - Vrije Universiteit Amsterdam

... Symbiogenesis • symbioses: fundamental organization of life based on syntrophy ranges from weak to strong interactions; basis of biodiversity • symbiogenesis: evolution of eukaryotes (mitochondria, plastids) • DEB model is closed under symbiogenesis: it is possible to model symbiogenesis of two ini ...
Table of Available Analyses - NHS Greater Glasgow and Clyde
Table of Available Analyses - NHS Greater Glasgow and Clyde

... Power Failure: In the event of power failure the pneumatic tube should not be used because it takes an hour to purge itself after restoration of power. HISS Requesting: Due to the complex range of tests performed, the HISS system offers over 500 biochemical tests. The following tables are a summary ...
Spain - Eurocare
Spain - Eurocare

... meconium. Evaluation of a possible cutoff is the next step  The sensitivity and specificity of EtG in hair is well-known and the determination of this metabolite in pregnant women’s hair could be very useful in the diagnosis of gestational ethanol exposure ...
Journal of Biotechnology Evaluation of 13C isotopic tracers for
Journal of Biotechnology Evaluation of 13C isotopic tracers for

... et al., 2007). Metabolic analysis of cancer cells has again become an active area of research, and technological improvements have expanded our ability to investigate metabolism using stable isotopically labeled substrates. In this study we used the A549 cancer cell line as a model system for evalua ...
Chemical (Elemental) Analysis - Fritz-Haber
Chemical (Elemental) Analysis - Fritz-Haber

... Ø a mass spectrometer separates a stream of gaseous ions into ions with different mass to charge ratio m/z (mass range in inorganic mass spectrometry from 1 – 300 u) Ø in combination with an ion source (ICP, spark, glow discharge, laser ablation) as analytical method for elemental analysis Ø most po ...
complete
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... Glycogen synthase – active when dephosphorylated, inactive when phosphorylated; insulin vs. glucagon ...
Evolution & organisation of metabolic Pathways
Evolution & organisation of metabolic Pathways

... Biomass: reserve(s) + structure(s) Reserve(s), structure(s): generalized compounds, mixtures of proteins, lipids, carbohydrates: fixed composition Reserve(s) do complicate model & implications & testing Reasons to delineate reserve, distinct from structure • metabolic memory • biomass composition d ...
Newsletter JUNE 2014
Newsletter JUNE 2014

... data is matched to the correct sample id. The panel can be used for the identification of exome, transcriptome, and whole genome sequenced samples. ...
Ontario`s Expanded Screening Program
Ontario`s Expanded Screening Program

...  Regional treatment centre will notify the infant’s healthcare provider and/or parents and arrange confirmatory testing  If diagnosis is confirmed, regional treatment centre will provide management counselling & follow up  Report will be mailed to referring hospital, provided that correct informa ...
Nutrition in the septic Patient
Nutrition in the septic Patient

... Critically Ill, Ventilated Patients Clearly, total caloric intake has a greater impact on CO2 production and respiratory function than does the ratio of CHO/fat (varying CHO content from 40-75% of total calories has little impact) Recommend 1.2 to 1.5 times REE (up to 5 mg/kg/min CHO infusion – 40 t ...
Catabolism of the branched
Catabolism of the branched

... • The metabolic block created by this defect leads to elevated levels of valine, leucine, and isoleucine (and their corresponding branched-chain a-keto acids) in the blood and urine. • The accumulation of the branched-chain α-keto acid substrates in urine give their sweet odor and prompted the name ...
Metabolism of Selenoamino Acids and Contribution of Selenium
Metabolism of Selenoamino Acids and Contribution of Selenium

... is metabolized by reduced glutathione (GSH) and/or glutathione reductase to hydrogen selenide (H2Se) via selenocysteine-glutathione selenenyl sulfide (CySeSG). The H2Se is a key intermediate in the methylation process of inorganic and organic selenium compounds. Accumulation of H2Se resulting from i ...
Human Metabolism: Macronutrients Instructors: Rosalind Coleman
Human Metabolism: Macronutrients Instructors: Rosalind Coleman

... Assignments/Discussion sessions/Questions: The objective of these sessions and the assignments is to help you use and integrate information covered in the course. These sessions will help you understand disorders related to nutrition. You may be asked to participate in class. If you do not wish to b ...
Guide to preparation of liquid biopsies for nucleic acid extraction
Guide to preparation of liquid biopsies for nucleic acid extraction

... 1.   Use of a suited and validated extraction method. Several commercial kits for nucleic acid extraction from body fluids are available. However, we recommend to use following extraction methods as they have been extensively tested and used in Biogazelle’s downstream applications: miRNeasy serum/pl ...
classes/nsg101/Acid Based balance student
classes/nsg101/Acid Based balance student

...  If the blood is alkalotic or acidotic, we now need to determine if it is caused primarily by a respiratory or metabolic problem. To do this, assess the PaCO2 level. Remember that with a respiratory problem, as the pH decreases below 7.35, the PaCO2 should rise. If the pH rises above 7.45, the PaCO ...
Regulation of metabolic pathways at the cellular level
Regulation of metabolic pathways at the cellular level

... Changing the absolute concentration of enzyme • Induction (activation) or repression (inhibition) of gene encoding the enzyme ...
Disposition of Glutathione Conjugates in Rats by a Novel Glutamic
Disposition of Glutathione Conjugates in Rats by a Novel Glutamic

... are not elucidated due to the difficulty in isolating and separating them from endogenous components in the biological matrices. With the advent of liquid chromatography/mass spectrometry (LC/MS) and LC/NMR, it has become possible to elucidate the structures of minor metabolites. Characterization of ...
Regulation of Primary Metabolism in Response to
Regulation of Primary Metabolism in Response to

... Based on enzyme activity assays and metabolic responses to waterlogging of the legume Lotus japonicus, it was previously suggested that, during hypoxia, the tricarboxylic acid cycle switches to a noncyclic operation mode. Hypotheses were postulated to explain the alternative metabolic pathways invol ...
Coordinated concentration changes of transcript and metabolites in Saccharomyces cerevisiae.
Coordinated concentration changes of transcript and metabolites in Saccharomyces cerevisiae.

... Metabolite concentrations can regulate gene expression, which can in turn regulate metabolic activity. The extent to which functionally related transcripts and metabolites show similar patterns of concentration changes, however, remains unestablished. We measure and analyze the metabolomic and trans ...
Molecular analysis in three cases of X91- variant chronic granulomatous disease
Molecular analysis in three cases of X91- variant chronic granulomatous disease

... mL reaction; 0.8 pCi of "'1); 2 mmol/L glucose; 3% normal serum; overlapping fragments (1-624, 539-1222, and 1127-1746 bp) using 0.5 mg of Zymosan boiled, washed, and resuspended in phosphatethree pairs of synthetic primerswith one primerof each pair biotinylbuffered saline (PBS), and 2.5 X 10' poly ...
Introduction to quantitative real
Introduction to quantitative real

... • Calculates efficiency for each individual sample based on rate of fluorescence change and mean efficiency across run • Calculates a “take off” value – start of exponential phase, less subjective than setting threshold for Ct • Set calibrator sample, T0 or untreated etc • Relative amount calculated ...
Detailed Supporting Information
Detailed Supporting Information

... triplicate cell cultures for each condition within each experiment and experiments were repeated once. Mass spectroscopic analyses were carried out by three independent automatic injections of 1µl samples by the automatic sampler and accepted only if the standard sample deviation was less than 1% of ...
Glycosylation of Proteins - Structure, Function and Analysis
Glycosylation of Proteins - Structure, Function and Analysis

... Glycosylation is one of the most widely observed, and structurally diverse, forms of post translational modification (PTM) of proteins. Animal, plant, fungal and bacterial cell systems all have the ability to glycosylate proteins and, whilst similarities do exist between these groups, there are also ...
An Introduction to Metabolism and Energetics
An Introduction to Metabolism and Energetics

... • Liver cells (primary sites of deamination) have enzymes that use © 2012 Pearson Education, Inc. ...
< 1 ... 36 37 38 39 40 41 42 43 44 ... 78 >

Pharmacometabolomics

Pharmacometabolomics, also known as pharmacometabonomics, is a field which stems from metabolomics, the quantification and analysis of metabolites produced by the body. It refers to the direct measurement of metabolites in an individual’s bodily fluids, in order to predict or evaluate the metabolism of pharmaceutical compounds, and to better understand the pharmacokinetic profile of a drug. Alternatively, pharmacometabolomics can be applied to measure metabolite levels following the administration of a pharmaceutical compound, in order to monitor the effects of the compound on certain metabolic pathways(pharmacodynamics). This provides detailed mapping of drug effects on metabolism and the pathways that are implicated in mechanism of variation of response to treatment. In addition, the metabolic profile of an individual at baseline (metabotype) provides information about how individuals respond to treatment and highlights heterogeneity within a disease state. All three approaches require the quantification of metabolites found in bodily fluids and tissue, such as blood or urine, and can be used in the assessment of pharmaceutical treatment options for numerous disease states.
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