Combined in silico modeling and metabolomics analysis to
... metabolomics and in silico modeling approach to gain a deeper insight into the cellular mechanisms of Chinese hamster ovary (CHO) fed-batch cultures. Initially, extracellular and intracellular metabolite profiling analysis shortlisted key metabolites associated with cell growth limitation within the ...
... metabolomics and in silico modeling approach to gain a deeper insight into the cellular mechanisms of Chinese hamster ovary (CHO) fed-batch cultures. Initially, extracellular and intracellular metabolite profiling analysis shortlisted key metabolites associated with cell growth limitation within the ...
field desorption (in mass spectrometry)
... identifying the resulting fragment ions. This information can then be pieced together to generate structural information regarding the intact molecule. Tandem mass spectrometry also enables specific compounds to be detected in complex mixtures on account of their specific and characteristic fragment ...
... identifying the resulting fragment ions. This information can then be pieced together to generate structural information regarding the intact molecule. Tandem mass spectrometry also enables specific compounds to be detected in complex mixtures on account of their specific and characteristic fragment ...
The Biochrom 30 series of Amino Acid Analysers
... Gel Electrophoresis Gel Electrophoresis remains one of the most important techniques in the life sciences. Biochrom, via its subsidiary Scie-Plas, offers a full range of electrophoresis products for analytical and preparative nucleic acid studies and manual DNA sequencing, including both horizontal ...
... Gel Electrophoresis Gel Electrophoresis remains one of the most important techniques in the life sciences. Biochrom, via its subsidiary Scie-Plas, offers a full range of electrophoresis products for analytical and preparative nucleic acid studies and manual DNA sequencing, including both horizontal ...
False-Positive and False-Negative Rates in Meconium Drug Testing
... and negative meconium specimens. The methods were not equivalent in their ability to extract drugs from the matrix. To determine the number of false positives reported by the use of screenonly (unconfirmed) results, 535 screen-positive meconium specimens were subjected to confirmation by gas chromat ...
... and negative meconium specimens. The methods were not equivalent in their ability to extract drugs from the matrix. To determine the number of false positives reported by the use of screenonly (unconfirmed) results, 535 screen-positive meconium specimens were subjected to confirmation by gas chromat ...
High-resolution analysis of metabolic cycles in the intertidal mussel
... quickly, and anaerobic metabolism commences at the onset of hypoxia (2). Under these hypoxic conditions, glucose and aspartate are fermented to produce succinate and alanine via the glucose-succinate and aspartate-succinate pathways, respectively (24). If the duration of hypoxia extends for days the ...
... quickly, and anaerobic metabolism commences at the onset of hypoxia (2). Under these hypoxic conditions, glucose and aspartate are fermented to produce succinate and alanine via the glucose-succinate and aspartate-succinate pathways, respectively (24). If the duration of hypoxia extends for days the ...
Determination of Fatty Acids and Carbohydrate Monomers in Micro
... study, found reproducible differences between chromatograms of M . avium, M. kansasii, M . tuberculosis and M . bovis, strain BCG. The latter two species produced virtually identical fatty acid GC profiles, and could only be distinguished when carbohydrates were also analysed. The 2 m packed column ...
... study, found reproducible differences between chromatograms of M . avium, M. kansasii, M . tuberculosis and M . bovis, strain BCG. The latter two species produced virtually identical fatty acid GC profiles, and could only be distinguished when carbohydrates were also analysed. The 2 m packed column ...
Determination of Fatty Acids and Carbohydrate Monomers in Micro
... study, found reproducible differences between chromatograms of M . avium, M. kansasii, M . tuberculosis and M . bovis, strain BCG. The latter two species produced virtually identical fatty acid GC profiles, and could only be distinguished when carbohydrates were also analysed. The 2 m packed column ...
... study, found reproducible differences between chromatograms of M . avium, M. kansasii, M . tuberculosis and M . bovis, strain BCG. The latter two species produced virtually identical fatty acid GC profiles, and could only be distinguished when carbohydrates were also analysed. The 2 m packed column ...
protein expression (pdf, en, 2701 KB, 4/8/10)
... ResidueResidue-specific enrichment It is achieved using E. coli auxotrophic strains which are grown in the presence of selected labeled aminoacids or using more sophisticated techniques such as cellcell-free. The latter approach allows to simplify problems due to protein size. It is possible to focu ...
... ResidueResidue-specific enrichment It is achieved using E. coli auxotrophic strains which are grown in the presence of selected labeled aminoacids or using more sophisticated techniques such as cellcell-free. The latter approach allows to simplify problems due to protein size. It is possible to focu ...
The Sticht Center on - Wake Forest Clinical and Translational
... • Yongmei Liu • Developed methodology to quantify transcriptome / methylome on large scale. • Working with a large team – identified gene modules involved cellular cholesterol transport to be associated with coronary calcification and diabetes • This R01 explores microRNA regulation of these gene mo ...
... • Yongmei Liu • Developed methodology to quantify transcriptome / methylome on large scale. • Working with a large team – identified gene modules involved cellular cholesterol transport to be associated with coronary calcification and diabetes • This R01 explores microRNA regulation of these gene mo ...
What is metabolic engineering?
... protein-protein interaction studies, ligand binding studies, or immunoassays • High-end quadruple TOF tandem mass spectrometers enable high-performance protein identification, epitope and phosphorylation mapping, and protein-interaction analyses. ...
... protein-protein interaction studies, ligand binding studies, or immunoassays • High-end quadruple TOF tandem mass spectrometers enable high-performance protein identification, epitope and phosphorylation mapping, and protein-interaction analyses. ...
1,2-Ethanediol, [1-2-Chlorophenyl]-, 2-carbama
... currently under clinical investigation for adjunctive treatment of epilepsy (Rogawski, 2006). In preclinical studies (data on file), this drug has demonstrated broad anticonvulsant activity in acute rodent seizure models and in the kindled rat model of partial epilepsy. It has shown efficacy in atte ...
... currently under clinical investigation for adjunctive treatment of epilepsy (Rogawski, 2006). In preclinical studies (data on file), this drug has demonstrated broad anticonvulsant activity in acute rodent seizure models and in the kindled rat model of partial epilepsy. It has shown efficacy in atte ...
MNG_CSF Collection and Processing
... 1) Request a CSF collection kit online at Order kits online at www.mnglabs.com/kits. Tube #3 contains antioxidants necessary to protect the sample from oxidation. One set of tubes is required per patient. The total CSF volume required is 4.5 milliters. If you do not have a CSF kit on hand: • Collect ...
... 1) Request a CSF collection kit online at Order kits online at www.mnglabs.com/kits. Tube #3 contains antioxidants necessary to protect the sample from oxidation. One set of tubes is required per patient. The total CSF volume required is 4.5 milliters. If you do not have a CSF kit on hand: • Collect ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 7. Precursors are chemicals directly incorporated with products. 8. Glucanase enzyme is used to disrupt yeast cells. 9. Conformational epitopes are formed by continuous sequence of amino acids in a protein. 10. Oral corticosteroids are also used in conditions of autoimmunity. III Complete the follow ...
... 7. Precursors are chemicals directly incorporated with products. 8. Glucanase enzyme is used to disrupt yeast cells. 9. Conformational epitopes are formed by continuous sequence of amino acids in a protein. 10. Oral corticosteroids are also used in conditions of autoimmunity. III Complete the follow ...
lecture notes ch40 Animal Structure and
... of food because they need energy for self-maintenance. Machines without fuel do not suffer damage from starvation because they do not use energy for self-maintenance. Of course, organisms also use energy for purposes not directly related to self-maintenance, like movement, or synthesizing chemicals, ...
... of food because they need energy for self-maintenance. Machines without fuel do not suffer damage from starvation because they do not use energy for self-maintenance. Of course, organisms also use energy for purposes not directly related to self-maintenance, like movement, or synthesizing chemicals, ...
Chemotaxonomic Metabolite Profiling of 62 Indigenous Plant
... comparing differences in properties of plant species, such as morphological [14], physiological [15], and chemical characteristics [16]. Among the various plant taxonomic methods, chemotaxonomy, a method based on differences in chemical compounds, is a useful tool for classification of plant species ...
... comparing differences in properties of plant species, such as morphological [14], physiological [15], and chemical characteristics [16]. Among the various plant taxonomic methods, chemotaxonomy, a method based on differences in chemical compounds, is a useful tool for classification of plant species ...
Electrophoretic techniques : general principles , supporting
... Subject to possible changes: check the web site of the Teacher or the Department/School for updates. ...
... Subject to possible changes: check the web site of the Teacher or the Department/School for updates. ...
12866_2017_1009_MOESM5_ESM
... Mix with the matrix solution of sinapinic acid (ca. 1 μL, 20 mg/mL in 50 % acetonitrile with 1 % TFA) Spot onto the MALDI target (ca. 1 μL) Dry in air ...
... Mix with the matrix solution of sinapinic acid (ca. 1 μL, 20 mg/mL in 50 % acetonitrile with 1 % TFA) Spot onto the MALDI target (ca. 1 μL) Dry in air ...
Ethylene Glycol Intoxication
... - severe metabolic acidosis (high anion gap) from glycolic acid accumulation - very high lactate (artefactual as there is high cross reactivity between lactate and glycolate in laboratory analysis) -> high lactate with oxidase method, less high with lactate dehydrogenase method - high osmolar gap - ...
... - severe metabolic acidosis (high anion gap) from glycolic acid accumulation - very high lactate (artefactual as there is high cross reactivity between lactate and glycolate in laboratory analysis) -> high lactate with oxidase method, less high with lactate dehydrogenase method - high osmolar gap - ...
Reading GuideChapter6_Tues
... Which of these three methods is how cells make ATP within a metabolic pathway such as glycolysis by the transfer of a phosphate group from an organic compound to ADP? Which process is the generation of ATP through oxidation/reduction reactions in the electron transport chain? Another key concept for ...
... Which of these three methods is how cells make ATP within a metabolic pathway such as glycolysis by the transfer of a phosphate group from an organic compound to ADP? Which process is the generation of ATP through oxidation/reduction reactions in the electron transport chain? Another key concept for ...
BOTANY DEPARTMENT - university of nairobi staff profiles
... Purpose The unit aims to provide fundamental concepts with a focus on description, organization, and functions of metabolic pathways of primary metabolites in microorganisms, plants and mammalian systems. Course Objectives 1. To deliver a concise knowledge and understanding of primary basic metaboli ...
... Purpose The unit aims to provide fundamental concepts with a focus on description, organization, and functions of metabolic pathways of primary metabolites in microorganisms, plants and mammalian systems. Course Objectives 1. To deliver a concise knowledge and understanding of primary basic metaboli ...
METABOLIC COMPARTMENTATION
... equivalent of 30 to 38 ATP molecules per molecule of glucose oxidized to carbon dioxide. Whatever the number, it is much greater than the net 2 molecules of ATP obtained from the anaerobic glycolysis of glucose to two molecules of lactic acid. ...
... equivalent of 30 to 38 ATP molecules per molecule of glucose oxidized to carbon dioxide. Whatever the number, it is much greater than the net 2 molecules of ATP obtained from the anaerobic glycolysis of glucose to two molecules of lactic acid. ...
Part 2
... technique that carries out separation in two dimensions. Proteins are separated based on their isoelectric points (pI) in the first dimension followed by SDS-PAGE in the second dimension, which separates proteins based on their molecular weight. 2. IPG strip: Commercially available immobilized pH gr ...
... technique that carries out separation in two dimensions. Proteins are separated based on their isoelectric points (pI) in the first dimension followed by SDS-PAGE in the second dimension, which separates proteins based on their molecular weight. 2. IPG strip: Commercially available immobilized pH gr ...
lecture1
... proteins) are broken down to simpler end-products such as C02, H20 and NH3 and is accompanied by the synthesis of ATP. Anabolism refers to biosynthetic processes in which simple precursor molecules are enzymatically converted into the molecular components of cells, such as nucleic acids, proteins, l ...
... proteins) are broken down to simpler end-products such as C02, H20 and NH3 and is accompanied by the synthesis of ATP. Anabolism refers to biosynthetic processes in which simple precursor molecules are enzymatically converted into the molecular components of cells, such as nucleic acids, proteins, l ...
The activity reaction core and plasticity of metabolic networks
... To examine the utilization and relative flux rates of each metabolic reaction in a wide range of simulated environmental conditions ...
... To examine the utilization and relative flux rates of each metabolic reaction in a wide range of simulated environmental conditions ...