The nuclear membrane in multidrug resistance
... lines after incubation with epirubicin 10 mg/ mL are shown in Fig. 1A,B. MGH-U1 cells showed a clear nuclear uptake pattern of epirubicin (Fig. 1A), whereas MGH-U1R cells showed characteristic nuclear sparing of epirubicin (Fig. 2B). After microinjection with epirubicin the sensitive cell lines MGH- ...
... lines after incubation with epirubicin 10 mg/ mL are shown in Fig. 1A,B. MGH-U1 cells showed a clear nuclear uptake pattern of epirubicin (Fig. 1A), whereas MGH-U1R cells showed characteristic nuclear sparing of epirubicin (Fig. 2B). After microinjection with epirubicin the sensitive cell lines MGH- ...
Esau`s Plant Anatomy - Wiley Online Library
... for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages. For general information on our other products and services or for technical support, please contact our Customer Care Department within the United States at (800 ...
... for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages. For general information on our other products and services or for technical support, please contact our Customer Care Department within the United States at (800 ...
From skin to the treatment of diseases the possibilities of iPS cell
... and the matched cells become very similar to each other (40). For therapeutic purposes of the iPSC technology, the chosen cell types have to be easily accessible in the patient. Skin cells like dermal fibroblasts, keratinocytes, dermal papilla cells or melanocytes can be easily isolated by punch bio ...
... and the matched cells become very similar to each other (40). For therapeutic purposes of the iPSC technology, the chosen cell types have to be easily accessible in the patient. Skin cells like dermal fibroblasts, keratinocytes, dermal papilla cells or melanocytes can be easily isolated by punch bio ...
Versus Nuclear Localized Estrogen Receptors in Breast Cancer Cells
... endogenous genes, namely pS2 and WISP2, genes that we knew to be robustly regulated by the WT-ER in these cells and in MCF-7 breast cancer cells (31–33). As shown in Fig. 5, WT-ER robustly stimulated pS2 and WISP2 gene expression in a time-dependent manner in response to E2. In contrast, we observed ...
... endogenous genes, namely pS2 and WISP2, genes that we knew to be robustly regulated by the WT-ER in these cells and in MCF-7 breast cancer cells (31–33). As shown in Fig. 5, WT-ER robustly stimulated pS2 and WISP2 gene expression in a time-dependent manner in response to E2. In contrast, we observed ...
AN OPTICAL-INDUCED PLATFORM FOR MULTIPLE GENES
... is the result obtained from a traditional electroporator. The capability of the developed platform for multiple plasmid transfection was also explored. Figure 4 shows the transfected 293T cells under fluorescent microscopy observation. The pEFGPC-1, pDsRed-express-1 and pECFP-H2B plasmids, which car ...
... is the result obtained from a traditional electroporator. The capability of the developed platform for multiple plasmid transfection was also explored. Figure 4 shows the transfected 293T cells under fluorescent microscopy observation. The pEFGPC-1, pDsRed-express-1 and pECFP-H2B plasmids, which car ...
The interplay between chromosome stability and cell cycle control
... and (C) seriation algorithms can inform models of chromosome stability. Laboratory experiments were used to identify 19 genes that when over-expressed cause chromosome instability in the yeast Saccharomyces cerevisiae. To better understand the molecular mechanisms by which these genes act, we explor ...
... and (C) seriation algorithms can inform models of chromosome stability. Laboratory experiments were used to identify 19 genes that when over-expressed cause chromosome instability in the yeast Saccharomyces cerevisiae. To better understand the molecular mechanisms by which these genes act, we explor ...
Attachment
... whether they exist as single cells, colonies of cells, or in multicellular form. There are two general types of cells: prokaryotic and eukaryotic. Prokaryotic cells are very tiny and do not have a membrane-bound nucleus and cell organelles. Bacteria and blue-green algae (cyano bacteria) are prokaryo ...
... whether they exist as single cells, colonies of cells, or in multicellular form. There are two general types of cells: prokaryotic and eukaryotic. Prokaryotic cells are very tiny and do not have a membrane-bound nucleus and cell organelles. Bacteria and blue-green algae (cyano bacteria) are prokaryo ...
Title Single-cell cDNA microarray profiling of complex biological
... 10-13, 16, 17]: the next three sections will focus on some of the applications to developmental biology in particular. In addition, this method has recently been applied to an mRNA-sequencing whole-transcriptome analysis with a slight modification, and shown to be able to identify various previously ...
... 10-13, 16, 17]: the next three sections will focus on some of the applications to developmental biology in particular. In addition, this method has recently been applied to an mRNA-sequencing whole-transcriptome analysis with a slight modification, and shown to be able to identify various previously ...
TOLL-LIKE RECEPTORS Toll-like receptors & Host
... Identified a protein she called “Toll” meaning “weird” Helps the Drosophila embryo to differentiate its top from its bottom (Neural tube development) ...
... Identified a protein she called “Toll” meaning “weird” Helps the Drosophila embryo to differentiate its top from its bottom (Neural tube development) ...
Reactive oxygen species (ROS) and defence response in
... depending on the interaction. Whereas Rbohderived ROS are positive regulators of the HR induced by an avirulent bacteria, they are negative regulators of the cell death after an The Arabidopsis NAPDH oxidase AtrbohD is avirulent oomycete (Torres et al., 2002). In responsible for the production of mo ...
... depending on the interaction. Whereas Rbohderived ROS are positive regulators of the HR induced by an avirulent bacteria, they are negative regulators of the cell death after an The Arabidopsis NAPDH oxidase AtrbohD is avirulent oomycete (Torres et al., 2002). In responsible for the production of mo ...
Mechanisms of the proliferation and differentiation of plant cells in
... genes for PCNA or cyclins), an attempf was made to isolate cDNAs that are expressed periodically during the plant cell cycle in synchronous cultures, For this purpose, both systems for ensuring synchronous division of periwinkle cells were utilized, Using the system in which synchrony is induced by ...
... genes for PCNA or cyclins), an attempf was made to isolate cDNAs that are expressed periodically during the plant cell cycle in synchronous cultures, For this purpose, both systems for ensuring synchronous division of periwinkle cells were utilized, Using the system in which synchrony is induced by ...
and G-CSF-dependent myeloid cell lines
... GM-2) only form colonies in response to GM-CSF or IL-3 but not in the presence of Epo or G-CSF. G-CSF dependent lines (G-l and G-2) only form colonies in response to G-CSF. These cells divide few times in the presence of IL-3 and then die. Morphological and cytochemical analyses of these cell lines ...
... GM-2) only form colonies in response to GM-CSF or IL-3 but not in the presence of Epo or G-CSF. G-CSF dependent lines (G-l and G-2) only form colonies in response to G-CSF. These cells divide few times in the presence of IL-3 and then die. Morphological and cytochemical analyses of these cell lines ...
Signal Transduction and Control of the Cell Cycle in Yeast
... cells that can no longer respond to the particular environmental cue. Characterizing the specific proteins that are affected by these mutations gives insights into the components of the entire pathway. One pathway that has been extensively studied as a model for understanding signal transduction in ...
... cells that can no longer respond to the particular environmental cue. Characterizing the specific proteins that are affected by these mutations gives insights into the components of the entire pathway. One pathway that has been extensively studied as a model for understanding signal transduction in ...
Smart thermoresponsive coatings and surfaces for tissue
... show obvious differences between different batches of plasma-deposited coatings, although the thickness of deposited polymer varied from batch to batch [10,30]. This shows that cell adhesion and proliferation do not seem to be sensitive to the grafted layer thickness of plasma-polymerized PNIPAAm su ...
... show obvious differences between different batches of plasma-deposited coatings, although the thickness of deposited polymer varied from batch to batch [10,30]. This shows that cell adhesion and proliferation do not seem to be sensitive to the grafted layer thickness of plasma-polymerized PNIPAAm su ...
High productivity machining cell showcases process control at IMTS
... the output of a manufacturing process using ‘tailgate’ inspection is not enough and, more often, too late to control all the variability in a manufacturing process. It is critical that checks and measurements are also made before, during and immediately after machining to control both common-cause a ...
... the output of a manufacturing process using ‘tailgate’ inspection is not enough and, more often, too late to control all the variability in a manufacturing process. It is critical that checks and measurements are also made before, during and immediately after machining to control both common-cause a ...
Chapter 36: Resource Acquisition and Transport in Vascular Plants
... -Internal “clock” in guard cells: the memory of daily activity of opening and closing of stomata; even for plants that kept in the dark place. 22. Explain how xerophytes reduce transpiration. --The Xerophytes are plants that adapted to deserts and other regions with little moisture. --Xerophytes red ...
... -Internal “clock” in guard cells: the memory of daily activity of opening and closing of stomata; even for plants that kept in the dark place. 22. Explain how xerophytes reduce transpiration. --The Xerophytes are plants that adapted to deserts and other regions with little moisture. --Xerophytes red ...
The molecular basis for selective assembly of the UBAP1
... domain (amino acids 17–63) (Agromayor et al., 2012; de Souza and Aravind, 2010; Stefani et al., 2011). Although we failed to express UBAP1(1-63)–strep (data not shown) a slightly longer Nterminal fragment, UBAP1(1-95)–strep, that incorporates an additional predicted a-helix (Fig. 2B), could be gener ...
... domain (amino acids 17–63) (Agromayor et al., 2012; de Souza and Aravind, 2010; Stefani et al., 2011). Although we failed to express UBAP1(1-63)–strep (data not shown) a slightly longer Nterminal fragment, UBAP1(1-95)–strep, that incorporates an additional predicted a-helix (Fig. 2B), could be gener ...
HIV Attachment & Entry: Insights into pathogenesis and
... protein that modulates the pH in the Golgi compartment Ion channel activity of Vpu may be required for proper virion maturation and assembly by protecting newly formed Env protein from premature conformational changes in the secretory pathway ...
... protein that modulates the pH in the Golgi compartment Ion channel activity of Vpu may be required for proper virion maturation and assembly by protecting newly formed Env protein from premature conformational changes in the secretory pathway ...
DairyNZ Farmfact 3-10 Somatic Cell Counts (SCC)
... increase following calving for several days before dropping to normal levels, they also tend to rise towards the end of lactation. Short term rises can occur due to under feeding, pregnancy testing, bulling activity and holding milk. These effects are usually more noticeable in cows with mastitis. ...
... increase following calving for several days before dropping to normal levels, they also tend to rise towards the end of lactation. Short term rises can occur due to under feeding, pregnancy testing, bulling activity and holding milk. These effects are usually more noticeable in cows with mastitis. ...
Note Guide - WordPress.com
... Forbs are important sources of protein and carotene during the growing season. Shrubs are important to maintain phosphorus and protein levels in winter (dormant season). Grasses are important sources of energy (structural CHO's) throughout the year. Forbs and shrubs may contain anti-quality ...
... Forbs are important sources of protein and carotene during the growing season. Shrubs are important to maintain phosphorus and protein levels in winter (dormant season). Grasses are important sources of energy (structural CHO's) throughout the year. Forbs and shrubs may contain anti-quality ...
An antibody raised to a maize auxin-binding protein has inhibitory
... The inhibition of occurrence of first divísion only affects a fraction of the protoplast population, this fraction being about one third of the total population at the highest IgG concentration tested. This is in contrast with the fact that anti-abpl IgGs at a ten fold lower concentration inhibits t ...
... The inhibition of occurrence of first divísion only affects a fraction of the protoplast population, this fraction being about one third of the total population at the highest IgG concentration tested. This is in contrast with the fact that anti-abpl IgGs at a ten fold lower concentration inhibits t ...
Hydrogen peroxide induces programmed cell death features in
... observable event was a condensation of chromatin manifesting as hyperchromasia of DNA stained with Hoechst 33258. Compared with control cells (Fig. 2A), cells treated with H2O2 showed chromatin condensed at the periphery of the nucleus (Fig. 2B: `pre-apoptotic nuclei'), or the appearance of micronuc ...
... observable event was a condensation of chromatin manifesting as hyperchromasia of DNA stained with Hoechst 33258. Compared with control cells (Fig. 2A), cells treated with H2O2 showed chromatin condensed at the periphery of the nucleus (Fig. 2B: `pre-apoptotic nuclei'), or the appearance of micronuc ...
Microbiology - The Student Room
... 13. For a viable count a known volume of organisms is added to agar plates, incubated and the colonies counted. It is assumed that one cell gives rise to one colony. This makes no allowance for clumping of cells so may cause an underestimate of numbers. For a viable count, Clumping may account for a ...
... 13. For a viable count a known volume of organisms is added to agar plates, incubated and the colonies counted. It is assumed that one cell gives rise to one colony. This makes no allowance for clumping of cells so may cause an underestimate of numbers. For a viable count, Clumping may account for a ...
The Principles Governing the Lung Structure and Function
... There is a unique feature in the lung; the airways form a continuum from the trachea to the alveoli. There are no interruptions or abrupt transitions, but rather a smooth gradual transformation from the wide, strong trachea, through bronchi and bronchioles, to the minuscule delicate alveoli. In the ...
... There is a unique feature in the lung; the airways form a continuum from the trachea to the alveoli. There are no interruptions or abrupt transitions, but rather a smooth gradual transformation from the wide, strong trachea, through bronchi and bronchioles, to the minuscule delicate alveoli. In the ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.