Arg305 of Streptomyces l-glutamate oxidase plays a crucial role for
... of LGOX resembles that of LAO [19]. A previous report by Pawelek et al. of the crystal structure of LAO interacted with o-aminobenzoate (AB) described that three AB molecules are visible within the funnel of the LAO-AB complex [23]. Likewise, the structure of LGOX interacted with ligand provide us w ...
... of LGOX resembles that of LAO [19]. A previous report by Pawelek et al. of the crystal structure of LAO interacted with o-aminobenzoate (AB) described that three AB molecules are visible within the funnel of the LAO-AB complex [23]. Likewise, the structure of LGOX interacted with ligand provide us w ...
Electron Transport and ATP Synthesis
... • Stalk causes ATP synthase to have three different conformations: open, loose, tight • In “tight” conformation, energy has been used to cause an energy conformation that favors ATP formation • 9 protons = 3 ATP (or 1 ATP/3 protons) ...
... • Stalk causes ATP synthase to have three different conformations: open, loose, tight • In “tight” conformation, energy has been used to cause an energy conformation that favors ATP formation • 9 protons = 3 ATP (or 1 ATP/3 protons) ...
The Synthesis and Degradation of Nucleotides
... B. Delineate the sequence of reactions (group additions) involved in the synthesis of a purine nucleotide. C. Detail the reactions leading from inosine-5´-monophosphate to adenosine-5´-monophosphate and inosine-5´-monophosphate to guanosine-5´-monophosphate. D. Note similarities among the reactions ...
... B. Delineate the sequence of reactions (group additions) involved in the synthesis of a purine nucleotide. C. Detail the reactions leading from inosine-5´-monophosphate to adenosine-5´-monophosphate and inosine-5´-monophosphate to guanosine-5´-monophosphate. D. Note similarities among the reactions ...
TFE3 contains two activation domains, one acidic and the other
... 50% of the residues are identical among all three proteins and 45% are identical between two of the three. The conserved region is contained within the 102 amino acid C-terminal portion of TFE3 shown to be sufficient for activation (Fig. 1 A). Interestingly, although the proline content in the highl ...
... 50% of the residues are identical among all three proteins and 45% are identical between two of the three. The conserved region is contained within the 102 amino acid C-terminal portion of TFE3 shown to be sufficient for activation (Fig. 1 A). Interestingly, although the proline content in the highl ...
Vmax Regulation through Domain and Subunit Changes. The Active
... although it is still solvent exposed; therefore, the catalytic reaction probably involves additional conformational changes. By comparing the inhibited with the uninhibited complex, it is possible to describe changes in conformation that are involved in the inhibitory signal transduction of serine. ...
... although it is still solvent exposed; therefore, the catalytic reaction probably involves additional conformational changes. By comparing the inhibited with the uninhibited complex, it is possible to describe changes in conformation that are involved in the inhibitory signal transduction of serine. ...
Oxidative Phosphorylation Goal: ATP Synthesis
... Problem 39 • How did these key experiments support the chemiosmotic theory of Peter Mitchell? – The pH of the intermembrane space is lower than the pH of the mitochondrial matrix. – Oxidative phosphorylation does not occur in mitochondrial preparations to which detergents have been added. – Lipi ...
... Problem 39 • How did these key experiments support the chemiosmotic theory of Peter Mitchell? – The pH of the intermembrane space is lower than the pH of the mitochondrial matrix. – Oxidative phosphorylation does not occur in mitochondrial preparations to which detergents have been added. – Lipi ...
Lipid Metabolism During Exercise
... plasma FFA from adipocytes (large > 50,000 kcals) intramuscular TG (2,000 -3,000 kcals) plasma TG (very small role during exercise in humans) 5.) Destabilizing effect on membranes High IMTG (obesity, type-II diabetes) linked with insulin resistance in muscle. ...
... plasma FFA from adipocytes (large > 50,000 kcals) intramuscular TG (2,000 -3,000 kcals) plasma TG (very small role during exercise in humans) 5.) Destabilizing effect on membranes High IMTG (obesity, type-II diabetes) linked with insulin resistance in muscle. ...
Lipid Metabolism During Exercise
... FFA Transport to Muscle Cells • Fatty Acids from adipose – transported in blood via Albumin – 3 per – brought to muscle cell at fatty acid binding receptor proteins – taken into muscle cell Triglycerides in blood (chylomicrons and VLDL) broken down by lipoprotein lipase in capillary of the muscle b ...
... FFA Transport to Muscle Cells • Fatty Acids from adipose – transported in blood via Albumin – 3 per – brought to muscle cell at fatty acid binding receptor proteins – taken into muscle cell Triglycerides in blood (chylomicrons and VLDL) broken down by lipoprotein lipase in capillary of the muscle b ...
Higher Human Biology unit 1 section 5 ENZYMES
... Tips - This is a quantitative experiment and requires great accuracy • Weigh out sodium phosphate to the nearest 0.01g • Rinse the boat with an eye wash bottle after adding beaker with 80ml of distilled water • Dissolve thoroughly and add 80ml to 100ml volumetric flask ...
... Tips - This is a quantitative experiment and requires great accuracy • Weigh out sodium phosphate to the nearest 0.01g • Rinse the boat with an eye wash bottle after adding beaker with 80ml of distilled water • Dissolve thoroughly and add 80ml to 100ml volumetric flask ...
reactants -> products. - University of San Diego Home Pages
... 5) Isomerases catalyze the isomerase reactions. Because these reactions have only one substrate and one product, they are among the simplest enzymatic reactions. ...
... 5) Isomerases catalyze the isomerase reactions. Because these reactions have only one substrate and one product, they are among the simplest enzymatic reactions. ...
Structure and mechanism of action of a novel
... members of Staphylococcus and Streptococcus species, some of which are important human pathogens. Consequently, it is possible that iPGMs could be a target for rational design of a novel antibiotic. Interestingly, some bacteria have genes for both an iPGM and a dPGM, but only one enzyme comprises th ...
... members of Staphylococcus and Streptococcus species, some of which are important human pathogens. Consequently, it is possible that iPGMs could be a target for rational design of a novel antibiotic. Interestingly, some bacteria have genes for both an iPGM and a dPGM, but only one enzyme comprises th ...
Structure and function of carbohydrate
... On Earth, the most abundant renewable carbon source is biomass. It is organic material that has stored energy through photosynthesis. The unexploited, but substantial sources of plant-based material are usually byproducts of forestry and agriculture, for example, cellulose, hemicellulose, lignin, an ...
... On Earth, the most abundant renewable carbon source is biomass. It is organic material that has stored energy through photosynthesis. The unexploited, but substantial sources of plant-based material are usually byproducts of forestry and agriculture, for example, cellulose, hemicellulose, lignin, an ...
HOW CELLS HARVEST ENERGY
... Catabolism of glucose Cellular respiration = catabolism of glucose to produce ATP 3 types of cellular respiration: o Aerobic respiration (nearly all organisms) o Anaerobic respiration (only a few types of bacteria) o Fermentation (when aerobic can’t work) A. Aerobic Respiration Requires O2 Occurs in ...
... Catabolism of glucose Cellular respiration = catabolism of glucose to produce ATP 3 types of cellular respiration: o Aerobic respiration (nearly all organisms) o Anaerobic respiration (only a few types of bacteria) o Fermentation (when aerobic can’t work) A. Aerobic Respiration Requires O2 Occurs in ...
Adv. Bio. Ch 9 Glyco and Resp
... and redox rnxs are not coupled – we round the number of ATP produced for each NADH to 3 and FADH2 2 (more like 2.5-3.3 and 1.5-2 respectively) NADH is hard to get in to the mitochondria from cytosol – shuttle to get it in varies by cell (NAD+ or FAD) – more ATP if shuttled in by NAD+ The ATP mad ...
... and redox rnxs are not coupled – we round the number of ATP produced for each NADH to 3 and FADH2 2 (more like 2.5-3.3 and 1.5-2 respectively) NADH is hard to get in to the mitochondria from cytosol – shuttle to get it in varies by cell (NAD+ or FAD) – more ATP if shuttled in by NAD+ The ATP mad ...
Bio 226: Cell and Molecular Biology
... 1) H+ translocation alters affinity of active site for ATP 2) Each active site ratchets through 3 conformations that have different affinities for ATP, ADP & Pi 3) ATP is synthesized by rotational catalysis g subunit rotates as H+ pass through Fo, forces each active site to sequentially adopt the 3 ...
... 1) H+ translocation alters affinity of active site for ATP 2) Each active site ratchets through 3 conformations that have different affinities for ATP, ADP & Pi 3) ATP is synthesized by rotational catalysis g subunit rotates as H+ pass through Fo, forces each active site to sequentially adopt the 3 ...
Spectroscopic Techniques: I Spectrophotometric Techniques
... they are masked by the more intense peptide bond absorption. The most useful range for proteins is above 230 nm, where there are absorptions from aromatic side chains. While a very weak absorption maximum of phenylalanine occurs at 257 nm, tyrosine and tryptophan dominate the typical protein spectru ...
... they are masked by the more intense peptide bond absorption. The most useful range for proteins is above 230 nm, where there are absorptions from aromatic side chains. While a very weak absorption maximum of phenylalanine occurs at 257 nm, tyrosine and tryptophan dominate the typical protein spectru ...
Document
... so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -KM and the x-intercept is Vmax ...
... so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -KM and the x-intercept is Vmax ...
Answers - U of L Class Index
... The ATP synthase extends through the inner mitochondrial membrane with the F0 part in contact with the proton gradient in the intermembrane space, while the F1 complex is in the matrix. ...
... The ATP synthase extends through the inner mitochondrial membrane with the F0 part in contact with the proton gradient in the intermembrane space, while the F1 complex is in the matrix. ...
NAD - wwphs
... Acetyl CoA enters mitochondria matrix and reacts with oxaloacetate Citrate (aka citric acid cycle) A series of reactions will yield oxaloacetate again (aka cycle) Each pyruvate makes 3 NADH, 1FADH2, 1ATP, 2 CO2 How many per glucose? ...
... Acetyl CoA enters mitochondria matrix and reacts with oxaloacetate Citrate (aka citric acid cycle) A series of reactions will yield oxaloacetate again (aka cycle) Each pyruvate makes 3 NADH, 1FADH2, 1ATP, 2 CO2 How many per glucose? ...
Chapter
... • An enzyme may also couple two different reactions. • Coupling an exothermic reaction with an endothermic one allows the enzyme to use the energy released by the exothermic reaction to drive the endothermic reaction. • In fact, a large variety of enzymes couple many different endothermic reactions ...
... • An enzyme may also couple two different reactions. • Coupling an exothermic reaction with an endothermic one allows the enzyme to use the energy released by the exothermic reaction to drive the endothermic reaction. • In fact, a large variety of enzymes couple many different endothermic reactions ...
enzymes lecture 1
... Active Site(catalytic site) • A restricted region of an enzyme molecule which binds to the substrate. Active Site • It is formed from Substrate Enzyme Amino acids sequences in the polypeptide chain . ...
... Active Site(catalytic site) • A restricted region of an enzyme molecule which binds to the substrate. Active Site • It is formed from Substrate Enzyme Amino acids sequences in the polypeptide chain . ...
Calvin Cycle
... Most plants, designated C3, fix CO2 initially via RuBP Carboxylase, yielding the 3-C 3-phosphoglycerate. Plants designated C4 have one cell type in which phosphoenolpyruvate (PEP) is carboxylated via the enzyme PEP Carboxylase, to yield the 4-C oxaloacetate. Oxaloacetate is converted to other 4- ...
... Most plants, designated C3, fix CO2 initially via RuBP Carboxylase, yielding the 3-C 3-phosphoglycerate. Plants designated C4 have one cell type in which phosphoenolpyruvate (PEP) is carboxylated via the enzyme PEP Carboxylase, to yield the 4-C oxaloacetate. Oxaloacetate is converted to other 4- ...
Electron Transport Chain - Dr-Manar-KSU
... By Oxidative phosphorylation By substrate-level phosphorylation ...
... By Oxidative phosphorylation By substrate-level phosphorylation ...
Chapter 15 Enzymes
... • An example is trypsin, a digestive enzyme. • It is synthesized and stored as trypsinogen, which has no enzyme activity. • It becomes active only after a six-amino acid fragment is hydrolyzed and removed from the N-terminal end of its chain. • Removal of this small fragment changes not only the pri ...
... • An example is trypsin, a digestive enzyme. • It is synthesized and stored as trypsinogen, which has no enzyme activity. • It becomes active only after a six-amino acid fragment is hydrolyzed and removed from the N-terminal end of its chain. • Removal of this small fragment changes not only the pri ...
BCHEM 253 – METABOLISM IN HEALTH AND DISEASES
... The general acid catalyst protonating the carbonyl group is His-95. The result is the enediol intermediate. His-95 then functions as a general base abstracting the hydrogen from the hydroxyl group with Glu-165 functioning as a general acid donating its proton to the C2 carbon forming G-3-P. ...
... The general acid catalyst protonating the carbonyl group is His-95. The result is the enediol intermediate. His-95 then functions as a general base abstracting the hydrogen from the hydroxyl group with Glu-165 functioning as a general acid donating its proton to the C2 carbon forming G-3-P. ...
Luciferase
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The name is derived from Lucifer, the root of which means 'light-bearer' (lucem ferre). One example is the firefly luciferase (EC 1.13.12.7) from the firefly Photinus pyralis. ""Firefly luciferase"" as a laboratory reagent often refers to P. pyralis luciferase although recombinant luciferases from several other species of fireflies are also commercially available.