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Lecture 16: Spherical Virus Structures

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...  Plating efficiency - counts made by plaque assays are always lower than counts made with electron microscope. Plating efficiency with bacteriophage is usually > 50% but with some animal viruses may be <1%  The plaque procedure may be used to prepare pure viral strains  Cell cultures may also be ...
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... into the cell and is replicated along with the host cell’s DNA.  Do NOT lyse the host cell away.  Viral DNA becomes part of the hosts DNA…(prophage.)  The prophage will remain this way for a varied amount of time.  Some “factors” will activate the prophage, and the cell will start making viruses ...
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Virus/Bacterial Worksheet
Virus/Bacterial Worksheet

... 1. Where is the genetic material in a T4 bacteriophage located? 2. In general, is the genetic material in a virus inside or outside the protein parts? 3. Why do you think the word virus, based on the Latin word for poison, was used for these structures? ...
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Master/PhD position in cell biology of virus infection at the University
Master/PhD position in cell biology of virus infection at the University

... Master/PhD position in cell biology of virus infection at the University of Cologne We offer a master and/or PhD position to join the DFG-project “Herpes simplex virus entry into skin or mucosa” with the possibility to study at the Graduate School for Biological Sciences at the Faculty of Mathematic ...
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Virus quantification



Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.
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