Defining characteristics of Tn5 Transposase non
... localization within the global DNA, most likely through a direct transfer mechanism, and that nonspecific DNA binding may play a role in the cis bias manifested by Tn5 transposition. ...
... localization within the global DNA, most likely through a direct transfer mechanism, and that nonspecific DNA binding may play a role in the cis bias manifested by Tn5 transposition. ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
Charge Transport in DNA - Insights from
... based on thermodynamic observations.[26] Since then, reports have been constantly appearing against,[27] as well as in favor of the thermally induced melting.[28] Importantly, the nature of the overstretched state of DNA probably depends on the rate of stretching. A dsDNA oligomer was reported to de ...
... based on thermodynamic observations.[26] Since then, reports have been constantly appearing against,[27] as well as in favor of the thermally induced melting.[28] Importantly, the nature of the overstretched state of DNA probably depends on the rate of stretching. A dsDNA oligomer was reported to de ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... chains. Therefore, the easy penetration of Hoechst into these flexible sites (not into the rigid double helix) in such samples could occur. While binding to cellular DNA, the Hoechst dye can block the DNA replication during cell division. Consequently, Hoechst at low concentrations could be applied, ...
... chains. Therefore, the easy penetration of Hoechst into these flexible sites (not into the rigid double helix) in such samples could occur. While binding to cellular DNA, the Hoechst dye can block the DNA replication during cell division. Consequently, Hoechst at low concentrations could be applied, ...
... abundance during stages of development in which the imprinting pattern switches from monoallelic to biallelic, as assayed by real-time PCR. (D) Methylated DNA immunoprecipitation (MeDIP) analysis of the Kcnq1 and Kcnq1ot1 promoter regions in sperm and 7.5 days post coitum (dpc) embryos. 5meC lane = ...
PDF
... They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The main advantage of optical tweezers is the ease with which single beads can be manipulated and carried around. In contrast, magnetic tweezers are at their best when working with a homogen ...
... They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The main advantage of optical tweezers is the ease with which single beads can be manipulated and carried around. In contrast, magnetic tweezers are at their best when working with a homogen ...
Epigenetics & Chromatin Xist through transcriptional control of Dnmt3a
... the inactive state is stably maintained through all subsequent cell generations (reviewed in [1]). X inactivation is triggered by the expression of the X inactive specific transcript (Xist), an unusual non-coding RNA that has the unique property of binding to and coating the chromosome from which it ...
... the inactive state is stably maintained through all subsequent cell generations (reviewed in [1]). X inactivation is triggered by the expression of the X inactive specific transcript (Xist), an unusual non-coding RNA that has the unique property of binding to and coating the chromosome from which it ...
Supporting Information Parfenov et al. 10.1073/pnas.1416074111
... P values (adjusted by the Benjamini–Hochberg procedure) less than 0.05 were considered as significantly differentially methylated or expressed. The sites and corresponding statistics for all CpG loci and genes can be found in Dataset S1, Tables S3 and S4. Additionally, we carried out an unsupervised ...
... P values (adjusted by the Benjamini–Hochberg procedure) less than 0.05 were considered as significantly differentially methylated or expressed. The sites and corresponding statistics for all CpG loci and genes can be found in Dataset S1, Tables S3 and S4. Additionally, we carried out an unsupervised ...
reproductive cell fate transition in plants - Development
... multicellular gametophytes, from which the gametes are derived, and during which epigenetic reprogramming takes place. Here we show that in the Arabidopsis female megaspore mother cell (MMC), cell fate transition is accompanied by large-scale chromatin reprogramming that is likely to establish an ep ...
... multicellular gametophytes, from which the gametes are derived, and during which epigenetic reprogramming takes place. Here we show that in the Arabidopsis female megaspore mother cell (MMC), cell fate transition is accompanied by large-scale chromatin reprogramming that is likely to establish an ep ...
Classification of colorectal cancer based on correlation of clinical
... syndrome or germ-line hemi-allelic methylation of MLH1.36 The over-representation of CTNNB1 mutations in MSI-H CRC cell lines37 is probably due to the fact that very few such cell lines are derived from sporadic MSI-H CRCs. Methylation of the APC promoter could fill the mutational gap in theory, but ...
... syndrome or germ-line hemi-allelic methylation of MLH1.36 The over-representation of CTNNB1 mutations in MSI-H CRC cell lines37 is probably due to the fact that very few such cell lines are derived from sporadic MSI-H CRCs. Methylation of the APC promoter could fill the mutational gap in theory, but ...
Youngson and Whitelaw, 2008
... the establishment of epigenetic state can be influenced by environmental factors (33, 40, 129). To ensure the totipotency of the zygote and to prevent perpetuation of abnormal epigenetic states, most gene regulatory, i.e., epigenetic, information is not transferred between generations. Several mechan ...
... the establishment of epigenetic state can be influenced by environmental factors (33, 40, 129). To ensure the totipotency of the zygote and to prevent perpetuation of abnormal epigenetic states, most gene regulatory, i.e., epigenetic, information is not transferred between generations. Several mechan ...
The presence of two UvrB subunits in the UvrAB complex ensures
... is expected to cause local melting of the DNA helix, thereby facilitating insertion of the b-hairpin of UvrB between the DNA strands. From mutational analysis of the b-hairpin it was proposed that UvrB scans the DNA for damage by trying to ¯ip nucleotides out of the DNA helix, thereby probing for di ...
... is expected to cause local melting of the DNA helix, thereby facilitating insertion of the b-hairpin of UvrB between the DNA strands. From mutational analysis of the b-hairpin it was proposed that UvrB scans the DNA for damage by trying to ¯ip nucleotides out of the DNA helix, thereby probing for di ...
Agarose Gel Electrophoresis
... changes the superhelical density of closed circular molecules by inducing positive supercoils. With increasing concentrations of ethidium bromide, negative supercoils are gradually removed, causing a concomitant decrease in the mobility of the DNA molecule. This occurs until a critical free-dye conc ...
... changes the superhelical density of closed circular molecules by inducing positive supercoils. With increasing concentrations of ethidium bromide, negative supercoils are gradually removed, causing a concomitant decrease in the mobility of the DNA molecule. This occurs until a critical free-dye conc ...
Agarose Gel Electrophoresis
... commonly incorporated into the gel and running buffer. The dye reduces the mobility of linear duplexes (Fig. 10.4.2D) and has a particularly pronounced effect on the mobility of closed circular DNA. Ethidium bromide changes the superhelical density of closed circular molecules by inducing positive s ...
... commonly incorporated into the gel and running buffer. The dye reduces the mobility of linear duplexes (Fig. 10.4.2D) and has a particularly pronounced effect on the mobility of closed circular DNA. Ethidium bromide changes the superhelical density of closed circular molecules by inducing positive s ...
Identification of Genetic and Epigenetic Risk Factors for Psoriasis
... thesis examination committee, Dr. Anne Bowcock. It is difficult to overstate my gratitude to Anne. It was such a great honor and pleasant to work with Anne and during the past 6 years, she has been giving me enthusiastic supervision in ensuring my academic, professional, financial, and moral well-be ...
... thesis examination committee, Dr. Anne Bowcock. It is difficult to overstate my gratitude to Anne. It was such a great honor and pleasant to work with Anne and during the past 6 years, she has been giving me enthusiastic supervision in ensuring my academic, professional, financial, and moral well-be ...
Fulltext PDF - Indian Academy of Sciences
... replication. A failure of methylation during cold exposure may lead to the demethylation of cytosines on the newly synthesized DNA strand. After a second round of replication, this would result in the double-stranded demethylation of DNA in one daughter cell. Because 5-azacytidine may have unintende ...
... replication. A failure of methylation during cold exposure may lead to the demethylation of cytosines on the newly synthesized DNA strand. After a second round of replication, this would result in the double-stranded demethylation of DNA in one daughter cell. Because 5-azacytidine may have unintende ...
RECOMBINANT DNA TECHNOLOGY AND BIOTECHNOLOGY
... mobility that is inversely proportional to the log10 of their molecular weight. However, circular forms of DNA migrate in agarose distinctly differently from linear DNAs of the same mass. Uncut plasmids will migrate more rapidly than the same plasmid when linearized and most preparations of uncut pl ...
... mobility that is inversely proportional to the log10 of their molecular weight. However, circular forms of DNA migrate in agarose distinctly differently from linear DNAs of the same mass. Uncut plasmids will migrate more rapidly than the same plasmid when linearized and most preparations of uncut pl ...
The legal, social and ethical controversy of the collection and
... ability to characterize one or more rare features of an individual’s genome, that is, their hereditary makeup. DNA contains the blueprints that are responsible for our cells, tissues, organs, and body [4, p.8]. In short it can be likened to “God’s signature” [6, p.259]. Every single human has a uniq ...
... ability to characterize one or more rare features of an individual’s genome, that is, their hereditary makeup. DNA contains the blueprints that are responsible for our cells, tissues, organs, and body [4, p.8]. In short it can be likened to “God’s signature” [6, p.259]. Every single human has a uniq ...
GENECLEAN® Kit
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... For any lower value of cPEG there is a pressure difference and hence a net force (outward) on the confining walls, because an insufficient amount of water has been drawn out of the DNA solution to lower its hydrostatic pressure to 1 atm. Let cPEG be such an intermediate value: 0,cPEG ,cPEG . Suppos ...
... For any lower value of cPEG there is a pressure difference and hence a net force (outward) on the confining walls, because an insufficient amount of water has been drawn out of the DNA solution to lower its hydrostatic pressure to 1 atm. Let cPEG be such an intermediate value: 0,cPEG ,cPEG . Suppos ...
Topologically Non-linked Circular Duplex DNA
... nevertheless remain associated with each other, as if they were physically locked together. It is not at all surprising that this observation has discouraged many from seriously considering any proposals for alternative structures for DNA in which the strands are not topologically linked. In order t ...
... nevertheless remain associated with each other, as if they were physically locked together. It is not at all surprising that this observation has discouraged many from seriously considering any proposals for alternative structures for DNA in which the strands are not topologically linked. In order t ...
Uracil in DNA – occurrence, consequences and repair
... involved, and timing in the cell cycle. In this review the emphasis will be on the enzymes that initiate the repair process, the DNA glycosylases. Uracil in DNA results from deamination of cytosine to uracil, creating a premutagenic U : G mispair, or from misincorporation of dUMP instead of dTMP dur ...
... involved, and timing in the cell cycle. In this review the emphasis will be on the enzymes that initiate the repair process, the DNA glycosylases. Uracil in DNA results from deamination of cytosine to uracil, creating a premutagenic U : G mispair, or from misincorporation of dUMP instead of dTMP dur ...
Activity Name - Science4Inquiry.com
... Inversion Mutation: reverse one section of bases (a whole gene) Original Strand: ...
... Inversion Mutation: reverse one section of bases (a whole gene) Original Strand: ...
Methylation of the Factor IX Gene is the Main Source of Mutations
... main sources of mutations in this gene, which repeatedly occur de novo and support the incidence of hemophilia В with a high frequency. Asymmetry of C T and GA transition mutations was found in a number of CG sites of the complementary DNA strands. It is a result of "silent" mutations, which usual ...
... main sources of mutations in this gene, which repeatedly occur de novo and support the incidence of hemophilia В with a high frequency. Asymmetry of C T and GA transition mutations was found in a number of CG sites of the complementary DNA strands. It is a result of "silent" mutations, which usual ...
DNA methylation
DNA methylation is a process by which methyl groups are added to DNA. Methylation modifies the function of the DNA, typically acting to suppress gene transcription. DNA methylation is essential for normal development and is associated with a number of key processes including genomic imprinting, X-chromosome inactivation, suppression of repetitive elements, and carcinogenesis.Two of DNA's four nucleotides, cytosine and adenine, can be methylated. Adenine methylation is restricted to prokaryotes.The rate of cytosine DNA methylation differs strongly between species: 14% of cytosines are methylated in Arabidopsis thaliana, 4% in Mus musculus, 2.3% in Escherichia coli, 0.03% in Drosophila, and virtually none (< 0.0002%) in yeast species.DNA methylation can stably alter the expression of genes in cells as cells divide and differentiate from embryonic stem cells into specific tissues. The resulting change is normally permanent and unidirectional, preventing a cell from reverting to a stem cell or converting into a different cell type. However, DNA methylation can be removed either passively, by dilution as cells divide, or by a faster, active, process. The latter process occurs via hydroxylation of the methyl groups that are to be removed, rather than by complete removal of methyl groups. DNA methylation is typically removed during zygote formation and re-established through successive cell divisions during development. Methylation modifications that regulate gene expression are usually heritable through mitotic cell division; some methylation is also heritable through the specialized meiotic cell division that creates egg and sperm cells, resulting in genomic imprinting. DNA methylation suppresses the expression of endogenous retroviral genes and other harmful stretches of DNA that have been incorporated into the host genome over time. DNA methylation also forms the basis of chromatin structure, which enables a single cell to grow into multiple organs or perform multiple functions. DNA methylation also plays a crucial role in the development of nearly all types of cancer.DNA methylation at the 5 position of cytosine has the specific effect of reducing gene expression and has been found in every vertebrate examined. In adult somatic cells (cells in the body, not used for reproduction), DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells, and has also been indicated in neural development.