Examination IV Key
... transcription requires primers while replication does not only one strand is transcribed while both strands are replicated transcription can occur in either direction while replication can only occur in one direction transcription involves polymerization 3´ to 5´ while replication involves 5´ to 3´ ...
... transcription requires primers while replication does not only one strand is transcribed while both strands are replicated transcription can occur in either direction while replication can only occur in one direction transcription involves polymerization 3´ to 5´ while replication involves 5´ to 3´ ...
We describe a method for the formation of hybrid
... site located downstream of the IFN-al coding sequence and probably contained entirely a2-specific coding sequences; they were not analyzed further. Forty-four of the remaining plasmids were sequenced between the upstream Bglll site and the EcoRI site. As shown in Fig. 2, 11 different crossover regio ...
... site located downstream of the IFN-al coding sequence and probably contained entirely a2-specific coding sequences; they were not analyzed further. Forty-four of the remaining plasmids were sequenced between the upstream Bglll site and the EcoRI site. As shown in Fig. 2, 11 different crossover regio ...
PCR (BASIC REQUIREMENT, copied from last semester lecture
... fragments. PCR differs from PCR in that it is performed in vitro by DNA polymerase and not in living cells. The PCR technique was developed by Kary Mullis, who was awarded Nobel Prize in 1993. The following components are needed for the PCR. (1) DNA template containing the DNA fragment to be amplifi ...
... fragments. PCR differs from PCR in that it is performed in vitro by DNA polymerase and not in living cells. The PCR technique was developed by Kary Mullis, who was awarded Nobel Prize in 1993. The following components are needed for the PCR. (1) DNA template containing the DNA fragment to be amplifi ...
Biology 212 General Genetics
... Compare the genotype of the most frequent class to the least frequent class and determine which marker changes places. ...
... Compare the genotype of the most frequent class to the least frequent class and determine which marker changes places. ...
DNA structure
... a. DNA polymerase extends the RNA primer in opposite directions using monomers present in the cell. b. DNA ligase joins together two adjacent strands of DNA c. Helicase unwinds/separates the two DNA strands c. RNA primase then adds a short complementary strand of RNA (a RNA primer) to each strand d. ...
... a. DNA polymerase extends the RNA primer in opposite directions using monomers present in the cell. b. DNA ligase joins together two adjacent strands of DNA c. Helicase unwinds/separates the two DNA strands c. RNA primase then adds a short complementary strand of RNA (a RNA primer) to each strand d. ...
AP Biology Deoxyribonucleic acid
... http://www.biology.arizona.edu/biochemistry/problem_sets/large_molecules/06t.html ...
... http://www.biology.arizona.edu/biochemistry/problem_sets/large_molecules/06t.html ...
Shedding Light on Nucleic Acids and DNA under - Beilstein
... Photochemistry in Living Cells Most of the processes in living organisms are exquisitely spatiotemporally regulated – and this is true at every level of organization. A cell is more than just the sum of its individual (non-interacting) constituents, a tissue is more than just an assembly of individu ...
... Photochemistry in Living Cells Most of the processes in living organisms are exquisitely spatiotemporally regulated – and this is true at every level of organization. A cell is more than just the sum of its individual (non-interacting) constituents, a tissue is more than just an assembly of individu ...
DNA polymerase
... • DNA is the hereditary material and contains all the information needed to build an organism. • It is a polymeric molecule made from discrete units called nucleotides. • Nucleotides link together to form a DNA strand at positions 3’ and 5’ ...
... • DNA is the hereditary material and contains all the information needed to build an organism. • It is a polymeric molecule made from discrete units called nucleotides. • Nucleotides link together to form a DNA strand at positions 3’ and 5’ ...
Note: all of these sentences are true.
... 23.Single-stranded DNA-binding (SSB) proteins has two functions,1. Keep the two strands of DNA separated 2. Protect the DNA from nucleases that degrade ssDNA. 24.Type I DNA topoisomerases, cut one strand of the DNA duplex and relax negative supercoiled DNA only. 25.Type II DNA topoisomerases, cut bo ...
... 23.Single-stranded DNA-binding (SSB) proteins has two functions,1. Keep the two strands of DNA separated 2. Protect the DNA from nucleases that degrade ssDNA. 24.Type I DNA topoisomerases, cut one strand of the DNA duplex and relax negative supercoiled DNA only. 25.Type II DNA topoisomerases, cut bo ...
THE GENETIC PROCESS CHAPTER 4
... The discussion thus far describes the conversion of DNA information for the synthesis of proteins. The discussion is incomplete without consideration of another important process, DNA replication. Replication is the process whereby a DNA molecule duplicates to yield identical DNA molecules. The dupl ...
... The discussion thus far describes the conversion of DNA information for the synthesis of proteins. The discussion is incomplete without consideration of another important process, DNA replication. Replication is the process whereby a DNA molecule duplicates to yield identical DNA molecules. The dupl ...
DNA Replication
... making copies of DNA would be based on its structure • They said if you peeled apart DNA down the middle it would be easy to copy • This is because all of the unpaired bases must be paired with a specific base ...
... making copies of DNA would be based on its structure • They said if you peeled apart DNA down the middle it would be easy to copy • This is because all of the unpaired bases must be paired with a specific base ...
Chpt8_RecombineDNA.doc
... At least four types of naturally occurring recombination have been identified in living organisms (Fig. 8.1). General or homologous recombination occurs between DNA molecules of very similar sequence, such as homologous chromosomes in diploid organisms. General recombination can occur throughout the ...
... At least four types of naturally occurring recombination have been identified in living organisms (Fig. 8.1). General or homologous recombination occurs between DNA molecules of very similar sequence, such as homologous chromosomes in diploid organisms. General recombination can occur throughout the ...
Unit 4
... direction. Simultaneous 5’ ----> 3’ synthesis of anti-parallel strands at a replication fork yields a continuous leading strand and short, discontinuous segments of lagging strand. The fragments are later joined together with the help of DNA ligase. DNA synthesis must start on the end of a primer, ( ...
... direction. Simultaneous 5’ ----> 3’ synthesis of anti-parallel strands at a replication fork yields a continuous leading strand and short, discontinuous segments of lagging strand. The fragments are later joined together with the help of DNA ligase. DNA synthesis must start on the end of a primer, ( ...
What is DNA sequencing
... band indicates that its particular dideoxynucleotide was added first to the labeled primer. In Figure 2, for example, the band that migrated the farthest was in the ddATP reaction mixture. Therefore, ddATP must have been added first to the primer, and its complementary base, thymine, must have been ...
... band indicates that its particular dideoxynucleotide was added first to the labeled primer. In Figure 2, for example, the band that migrated the farthest was in the ddATP reaction mixture. Therefore, ddATP must have been added first to the primer, and its complementary base, thymine, must have been ...
Virginia Gil
... 8. Describe the structure of DNA, and explain what kind of chemical bond connects the nucleotides of each strand and what type of bond holds the two strands together. Each nucleotide unit of the polynucleotide chain consists of a nitrogenous base (T, A, C, OR G), the sugar deoxiribose, and a phospha ...
... 8. Describe the structure of DNA, and explain what kind of chemical bond connects the nucleotides of each strand and what type of bond holds the two strands together. Each nucleotide unit of the polynucleotide chain consists of a nitrogenous base (T, A, C, OR G), the sugar deoxiribose, and a phospha ...
DNA notes
... • 1907…Thomas Hunt Morgan works with fruit flies to show that chromosomes have a definite function in heredity. This work also establishes mutation theory and leads to an understanding of how inheritance works. ...
... • 1907…Thomas Hunt Morgan works with fruit flies to show that chromosomes have a definite function in heredity. This work also establishes mutation theory and leads to an understanding of how inheritance works. ...
DNA replication
... Chromosomes also must be attached to the mitotic spindle for mitosis to complete (failure to attach can result in nondisjunction). ...
... Chromosomes also must be attached to the mitotic spindle for mitosis to complete (failure to attach can result in nondisjunction). ...
Lecture 14: Nucleic Acids and DNA Replication
... DNA is a helix with a uniform width of 2 nm. Each nucleic acid strand has a width of 1nm, suggesting that DNA is double stranded Purine and pyrimidine bases are stacked 0.34 nm apart The helix makes one full turn each 3.4 nm along its length Each turn includes ten layers (10 x 0.34 = 3.4 nm) of nitr ...
... DNA is a helix with a uniform width of 2 nm. Each nucleic acid strand has a width of 1nm, suggesting that DNA is double stranded Purine and pyrimidine bases are stacked 0.34 nm apart The helix makes one full turn each 3.4 nm along its length Each turn includes ten layers (10 x 0.34 = 3.4 nm) of nitr ...
chapter 8 and 9
... Example: nitrous acid strips the amino group from nucleotides Base analogs Resemble nucleotide bases; erroneously incorporated into DNA Analog base-pairs with a different nucleotide Intercalating agents Insert between base-pairs, pushing nucleotides apart; extra nucleotide may then be erroneously ad ...
... Example: nitrous acid strips the amino group from nucleotides Base analogs Resemble nucleotide bases; erroneously incorporated into DNA Analog base-pairs with a different nucleotide Intercalating agents Insert between base-pairs, pushing nucleotides apart; extra nucleotide may then be erroneously ad ...
Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology
... Charles McHenry (CU SOM) biochemical studies ...
... Charles McHenry (CU SOM) biochemical studies ...
File - Groby Bio Page
... The strands are separated according to size in a process similar to gel electrophoresis. A computer analyses the order of fluorescent markers, and can therefore deduce the order of bases in the template DNA strand. ...
... The strands are separated according to size in a process similar to gel electrophoresis. A computer analyses the order of fluorescent markers, and can therefore deduce the order of bases in the template DNA strand. ...
Molecular Biology Fourth Edition
... structures from local base-pairing interactions, e.g. mRNA. • These RNAs may fold into three-dimensional structures only in the presence of bound proteins, forming complexes called ribonucleoproteins (RNPs). ...
... structures from local base-pairing interactions, e.g. mRNA. • These RNAs may fold into three-dimensional structures only in the presence of bound proteins, forming complexes called ribonucleoproteins (RNPs). ...
Holliday junction
A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.