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... • This sequence is referred to as an Alu sequence after a restriction enzyme site that is located within this 300 base pair length of DNA. ...
... • This sequence is referred to as an Alu sequence after a restriction enzyme site that is located within this 300 base pair length of DNA. ...
Document
... 2. RNA nucleotides contain the fivecarbon sugar ribose rather than the sugar deoxyribose, which is found in DNA nucleotides 3. In addition to the A, G, and C nitrogen bases found in DNA, RNA nucleotides can have a nitrogen base called uracil (U) ...
... 2. RNA nucleotides contain the fivecarbon sugar ribose rather than the sugar deoxyribose, which is found in DNA nucleotides 3. In addition to the A, G, and C nitrogen bases found in DNA, RNA nucleotides can have a nitrogen base called uracil (U) ...
Sir Alec Jeffreys minisatellites
... CODIS - Repetitive DNA Minisatellite DNA Unit - 15-400 bp (average about 20). Repeat - Generally 20-50 times (1000-5000 bp long). Location - Generally euchromatic. Examples - DNA fingerprints. Tandemly repeated but often in ...
... CODIS - Repetitive DNA Minisatellite DNA Unit - 15-400 bp (average about 20). Repeat - Generally 20-50 times (1000-5000 bp long). Location - Generally euchromatic. Examples - DNA fingerprints. Tandemly repeated but often in ...
Restriction Enzymes, Gel Electrophoresis and Mapping DNA
... • Hybridization kinetics—complexity of regions of DNA, no specifics ...
... • Hybridization kinetics—complexity of regions of DNA, no specifics ...
Ch. 12 Notes
... Cytosine Guanine GuanineCytosine • Adenine Uracil Uracil Adenine • Thymine (from DNA) Adenine • DNA Strand: A T G C T A A G C ...
... Cytosine Guanine GuanineCytosine • Adenine Uracil Uracil Adenine • Thymine (from DNA) Adenine • DNA Strand: A T G C T A A G C ...
No Slide Title
... • Utilizes microbiological selection and screening procedures to isolate a gene that represents as little as 1 part in a million of the genetic material in an organism. ...
... • Utilizes microbiological selection and screening procedures to isolate a gene that represents as little as 1 part in a million of the genetic material in an organism. ...
File
... 2. describe how proteins are formed and what they are composed of 3. be aware of amino acids categorizations 4. draw a model to show the basic structure on a nucleotide 5. explain the bonds between consecutive nucleotides and the bonds between the bases, and explain their relative strengths. 6. stat ...
... 2. describe how proteins are formed and what they are composed of 3. be aware of amino acids categorizations 4. draw a model to show the basic structure on a nucleotide 5. explain the bonds between consecutive nucleotides and the bonds between the bases, and explain their relative strengths. 6. stat ...
2009 - Barley World
... phenotype in the F1 and F2 generations. You observe that all F1 plants are resistant and in the F2 there is a ratio of 15 resistant: 1susceptible. The most likely explanation for these observations is that a. the alleles at the resistance locus show codominance. b. the two varieties have the same re ...
... phenotype in the F1 and F2 generations. You observe that all F1 plants are resistant and in the F2 there is a ratio of 15 resistant: 1susceptible. The most likely explanation for these observations is that a. the alleles at the resistance locus show codominance. b. the two varieties have the same re ...
handout 1
... The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. We perform a number of these classic diagnostic test ...
... The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. We perform a number of these classic diagnostic test ...
clicker review
... B Polymerase chain reaction (PCR) C electroporation D gel electrophoresis E restriction fragment ligation 21. In recombinant gene technology, a vector can refer to A a plasmid used to transfer DNA into a living cell B an RFLP marker C the enzyme that cuts DNA into restriction fragments D the sticky ...
... B Polymerase chain reaction (PCR) C electroporation D gel electrophoresis E restriction fragment ligation 21. In recombinant gene technology, a vector can refer to A a plasmid used to transfer DNA into a living cell B an RFLP marker C the enzyme that cuts DNA into restriction fragments D the sticky ...
Lab 12
... different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in the DNA, the new DNA will generate more or less fragments/different sized fragments when cut with a particular enzyme ...
... different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in the DNA, the new DNA will generate more or less fragments/different sized fragments when cut with a particular enzyme ...
DNA Replication and DNA Repair Study Guide Focus on the
... iii. Eukaryotes- 1 to 2000 origins of replication per chromosome b. Direction- two forks proceed in opposite directions c. Forks i. Replication sites ii. Proceed in one direction (one for each direction) iii. Replication can only proceed in ϱ͛ƚŽϯ͛ĚŝƌĞĐƚŝŽŶ ...
... iii. Eukaryotes- 1 to 2000 origins of replication per chromosome b. Direction- two forks proceed in opposite directions c. Forks i. Replication sites ii. Proceed in one direction (one for each direction) iii. Replication can only proceed in ϱ͛ƚŽϯ͛ĚŝƌĞĐƚŝŽŶ ...
WWTBAM Review C8 test - Week of 1/12-1/15
... Suppose you can read the sequence of bases on only one strand of the double helix. What would you use to figure out the sequence on the other strand? ...
... Suppose you can read the sequence of bases on only one strand of the double helix. What would you use to figure out the sequence on the other strand? ...
DNA bracelet activity pack
... Today we are going to make a DNA bracelet just for you that is a copy of the pattern sequence of DNA held inside your genes. Does anyone know what a gene is? Not to be confused with a pair of trousers! A gene is a tiny code of genetic information that gives your body instructions. We all have genes ...
... Today we are going to make a DNA bracelet just for you that is a copy of the pattern sequence of DNA held inside your genes. Does anyone know what a gene is? Not to be confused with a pair of trousers! A gene is a tiny code of genetic information that gives your body instructions. We all have genes ...
Sex linked inheritance, sex linkage in Drosophila and man, XO, XY
... agents proflavin, acridine orange, and ICR-191. (b) An intercalating agent slips between the nitrogenous bases stacked at the center of the DNA molecule. This occurrence can lead to single-nucleotide-pair insertions and deletions. ...
... agents proflavin, acridine orange, and ICR-191. (b) An intercalating agent slips between the nitrogenous bases stacked at the center of the DNA molecule. This occurrence can lead to single-nucleotide-pair insertions and deletions. ...
Multiple Choice. ______1. Which of the following molecules
... c. genetic information is used to make proteins. d. sunlight energy is converted into chemical energy. ______39. Transcription of eukaryotic genes requires a. binding of RNA polymerase to the promoter. b. binding of several transcription factors. c. capping of mRNA. d. Both a and b ______40. The exp ...
... c. genetic information is used to make proteins. d. sunlight energy is converted into chemical energy. ______39. Transcription of eukaryotic genes requires a. binding of RNA polymerase to the promoter. b. binding of several transcription factors. c. capping of mRNA. d. Both a and b ______40. The exp ...
DNA upgrade supplement WITH PICS
... sugar molecules. This helps to describe molecules and discuss where specific bonds form. For instance the DNA sugar, deoxyribose, is easily distinguished from the RNA sugar, ribose, because deoxyribose lacks an oxygen atom at the number two position in the ring. The phosphodiester bonds Levene descr ...
... sugar molecules. This helps to describe molecules and discuss where specific bonds form. For instance the DNA sugar, deoxyribose, is easily distinguished from the RNA sugar, ribose, because deoxyribose lacks an oxygen atom at the number two position in the ring. The phosphodiester bonds Levene descr ...
Cloning vectors share four common properties
... Many cloning vectors contain a multiple cloning site or polylinker: a DNA segment with several unique sites for restriction endo- nucleases located next to each other Restriction sites of the polylinker are not present anywhere else in the plasmid. Cutting plasmids with one of the restriction enzyme ...
... Many cloning vectors contain a multiple cloning site or polylinker: a DNA segment with several unique sites for restriction endo- nucleases located next to each other Restriction sites of the polylinker are not present anywhere else in the plasmid. Cutting plasmids with one of the restriction enzyme ...
Lab 4 Restriction Analysis
... RE's first made it possible to work with small, defined pieces of DNA. Before RE's were discovered, a scientist might be able to tell that a chromosome contained a gene of interest to him. He might be able to purify the protein or use genetic analysis to tell what other genes were close to "his" gen ...
... RE's first made it possible to work with small, defined pieces of DNA. Before RE's were discovered, a scientist might be able to tell that a chromosome contained a gene of interest to him. He might be able to purify the protein or use genetic analysis to tell what other genes were close to "his" gen ...
5. Nucleic Acids-Structure, Central Dogma – Bio 20
... This difference in structure affects secondary structure and stability. ...
... This difference in structure affects secondary structure and stability. ...
Chapter 12 Molecular Genetics
... Double helix Deoxyribose sugar Bases: adenine, guanine, cytosine, and thymine One type of DNA ...
... Double helix Deoxyribose sugar Bases: adenine, guanine, cytosine, and thymine One type of DNA ...
DNA TEST
... c) Protein; nucleic acid b) DNA; RNA d) Nuclein; protein 3. The person/team that developed the first photograph of the helical molecule with its bases inside were ...
... c) Protein; nucleic acid b) DNA; RNA d) Nuclein; protein 3. The person/team that developed the first photograph of the helical molecule with its bases inside were ...
DNA
Deoxyribonucleic acid (/diˌɒksiˌraɪbɵ.njuːˌkleɪ.ɨk ˈæsɪd/; DNA) is a molecule that carries most of the genetic instructions used in the development, functioning and reproduction of all known living organisms and many viruses. DNA is a nucleic acid; alongside proteins and carbohydrates, nucleic acids compose the three major macromolecules essential for all known forms of life. Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix. The two DNA strands are known as polynucleotides since they are composed of simpler units called nucleotides. Each nucleotide is composed of a nitrogen-containing nucleobase—either cytosine (C), guanine (G), adenine (A), or thymine (T)—as well as a monosaccharide sugar called deoxyribose and a phosphate group. The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. According to base pairing rules (A with T, and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. The total amount of related DNA base pairs on Earth is estimated at 5.0 x 1037, and weighs 50 billion tonnes. In comparison, the total mass of the biosphere has been estimated to be as much as 4 TtC (trillion tons of carbon).DNA stores biological information. The DNA backbone is resistant to cleavage, and both strands of the double-stranded structure store the same biological information. Biological information is replicated as the two strands are separated. A significant portion of DNA (more than 98% for humans) is non-coding, meaning that these sections do not serve as patterns for protein sequences.The two strands of DNA run in opposite directions to each other and are therefore anti-parallel. Attached to each sugar is one of four types of nucleobases (informally, bases). It is the sequence of these four nucleobases along the backbone that encodes biological information. Under the genetic code, RNA strands are translated to specify the sequence of amino acids within proteins. These RNA strands are initially created using DNA strands as a template in a process called transcription.Within cells, DNA is organized into long structures called chromosomes. During cell division these chromosomes are duplicated in the process of DNA replication, providing each cell its own complete set of chromosomes. Eukaryotic organisms (animals, plants, fungi, and protists) store most of their DNA inside the cell nucleus and some of their DNA in organelles, such as mitochondria or chloroplasts. In contrast, prokaryotes (bacteria and archaea) store their DNA only in the cytoplasm. Within the chromosomes, chromatin proteins such as histones compact and organize DNA. These compact structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed.First isolated by Friedrich Miescher in 1869 and with its molecular structure first identified by James Watson and Francis Crick in 1953, DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity. The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. Among notable advances in this field are DNA origami and DNA-based hybrid materials.The obsolete synonym ""desoxyribonucleic acid"" may occasionally be encountered, for example, in pre-1953 genetics.