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DEVELOPMENT AND CHARACTERIZATION OF SULFIDE-OXIDIZING BIOFILMS UNIVERSITAT AUTÒNOMA DE BARCELONA Facultat de Ciències
DEVELOPMENT AND CHARACTERIZATION OF SULFIDE-OXIDIZING BIOFILMS UNIVERSITAT AUTÒNOMA DE BARCELONA Facultat de Ciències

... of all, several DNA extraction procedures were evaluated in order to select the most suitable method for performing the diversity analysis of our biofilms. We compared the extraction efficiency (i.e. amount of DNA recovered), as well as the genetic diversity recovered by denaturing gradient gel elec ...
Bacteriophage functional genomics and its role in
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... remnants of formerly functional temperate bacteriophages. In addition, some comparisons may have been skewed since Phage_Finder can run under two different stringency modes, producing radically different results. PhiSpy [56] uses a training set based on known prophages in closely related organisms t ...
investigation of the function and structure of ace2p
investigation of the function and structure of ace2p

... Larvae became inactive, showed unusual behaviour, failed to pupate and died. Larvae were submitted to the Faculty of Veterinary Science to diagnose the aetiology of the disease. On the basis of clinical signs, virus infections were suspected. Tissue homogenates of diseased pupae were subjected to ne ...
The bactericidal mechanism of the complement membrane attack
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... in serum and body fluids.1 Functionally, the complement system can be divided into three parts. The system can mark (opsonize) pathogens for phagocytic cells, it can stimulate inflammatory reactions and it can kill Gram-negative bacteria by the surface-deposition of the membrane attack complex (MAC) ...
Syllabus of M.Sc Microbiology of Annamalai University
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... who has failed to obtain the prescribed 80% attendance for valid reasons on payment of a condonation fee and such exemption should not under any circumstances be granted for attendance below 70%. Examination There will be two sessional assessments and one end semester Examinations during each semest ...
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... 9. Preparation of media (Nutrient broth, Nutrient agar). 10. Isolation of pure culture. 11. Enumeration of microbial cells (pour plate method). 12. Fungal staining. 13. Fungal Culture. 14. Determination of phenol coefficient. 15. Use of differential and selective media. 16. Oligodynamic action of he ...
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... 7% instead of 5% sucrose and incubated at 30 deg C, or subculturing 30-day-old colonies on NSA, resulted in the appearance of clear, waterdrop-like colony variants. These variants did not induce the hypersensitivity reaction in tobacco leaves. This colony type was occasionally observed on NSA during ...
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... probably the most important and widely used microbiological differential stain. Medical Applications The Gram stain is the first differential test run on a specimen brought into the laboratory for identification. Principle The Gram stain is a differential stain in which a decolorization step occurs ...
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... and most HAI’s are accorded only a limited significance (Hambraeus, 2006). Correspondingly, it was found that the predominant organisms responsible for nosocomial infections in technically advanced countries were gram positive cocci, while gram negative bacilli were the major cause of HAI’s in devel ...
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... 116). Many of these activities are involved in virulence and for this reason have been studied in the context of pathogenesis, but microbial social behaviors are important in a variety of other contexts (24). One type of social trait that has been studied extensively at the molecular level is the ab ...
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When saliva meets acid: chemical warfare at the
When saliva meets acid: chemical warfare at the

... from the mucosa near to the oesophagogastric junction. The fall in incidence of carcinoma of the mid and distal stomach may be explained by the falling incidence of Helicobacter pylori infection and associated atrophic gastritis which are major risk factors for cancer at these sites. However, the mo ...
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... help to build proteins from the feed and manufacture all of the B vitamins needed by the goat. Many nutrients that help provide the goat with energy are also absorbed here. „ Two primary types of ...
Chapter 1
Chapter 1

... • study of organisms too small to be clearly seen by the unaided eye (i.e., microorganisms) • these organisms are relatively simple in their construction and lack highly differentiated cells and distinct ...
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Human microbiota



The human microbiota is the aggregate of microorganisms, a microbiome that resides on the surface and in deep layers of skin (including in mammary glands), in the saliva and oral mucosa, in the conjunctiva, and in the gastrointestinal tracts. They include bacteria, fungi, and archaea. Micro-animals which live on the human body are excluded. The human microbiome refer to their genomes.One study indicated they outnumber human cells 10 to 1. Some of these organisms perform tasks that are useful for the human host. However, the majority have been too poorly researched for us to understand the role they play, however communities of microflora have been shown to change their behavior in diseased individuals. Those that are expected to be present, and that under normal circumstances do not cause disease, but instead participate in maintaining health, are deemed members of the normal flora. Though widely known as microflora, this is a misnomer in technical terms, since the word root flora pertains to plants, and biota refers to the total collection of organisms in a particular ecosystem. Recently, the more appropriate term microbiota is applied, though its use has not eclipsed the entrenched use and recognition of flora with regard to bacteria and other microorganisms. Both terms are being used in different literature.Studies in 2009 questioned whether the decline in biota (including microfauna) as a result of human intervention might impede human health.Most of the microbes associated with humans appear to be not harmful at all, but rather assist in maintaining processes necessary for a healthy body. A surprising finding was that at specific sites on the body, a different set of microbes may perform the same function for different people. For example, on the tongues of two people, two entirely different sets of organisms will break down sugars in the same way. This suggests that medical science may be forced to abandon the ""one only"" microbe model of infectious disease, and rather pay attention to functions of groups of microbes that have somehow gone awry.
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