Evaluation of Genotypic variation using SDS-PAGE
... bands were up- regulated whereas some other bands showed down regulation. On the basis of their studies they concluded that the identified protein patterns may be used as protein markers. ...
... bands were up- regulated whereas some other bands showed down regulation. On the basis of their studies they concluded that the identified protein patterns may be used as protein markers. ...
Targeted Proteomic Study of the Cyclin
... Clb3 (Supplemental Figure S1 on Molecular Cell’s website) was observed, most likely because of its role in targeting this cyclin for degradation (Schwab et al., 2001; Visintin et al., 1997). The association of Cdc4 and Cdc53 with Clb3 (Supplemental Figure S1 on Molecular Cell’s website), could have ...
... Clb3 (Supplemental Figure S1 on Molecular Cell’s website) was observed, most likely because of its role in targeting this cyclin for degradation (Schwab et al., 2001; Visintin et al., 1997). The association of Cdc4 and Cdc53 with Clb3 (Supplemental Figure S1 on Molecular Cell’s website), could have ...
Measuring Enzyme Activity Using the Thermo Scientific NanoDrop
... researchers. They may be used as reporter groups of fluorogenic substrates needed for the determination of enzyme activities. These substrates are nonfluorescent in their initial “structure” and only through enzymatic cleavage have their fluorescent properties become apparent. Thereby, they are very ...
... researchers. They may be used as reporter groups of fluorogenic substrates needed for the determination of enzyme activities. These substrates are nonfluorescent in their initial “structure” and only through enzymatic cleavage have their fluorescent properties become apparent. Thereby, they are very ...
Heart Failure
... reported previously,15 7 days of sTAB was sufficient to induce clinical heart failure as demonstrated by development of robust cardiac hypertrophy, pulmonary edema, and diminished systolic function (Figure 1). Elevated afterload imposes oxidative, biomechanical, and neurohumoral stress on the heart, ...
... reported previously,15 7 days of sTAB was sufficient to induce clinical heart failure as demonstrated by development of robust cardiac hypertrophy, pulmonary edema, and diminished systolic function (Figure 1). Elevated afterload imposes oxidative, biomechanical, and neurohumoral stress on the heart, ...
Interaction of a 14-3-3 protein with the plant
... Subcellular localization of green fluorescent protein (GFP) – EDE1 fusions on the microtubules of the spindle and spindle poles during mitosis and on the phragmoplast during cytokinesis indicates that EDE1 has a key role in microtubule function during mitosis. Also, its expression pattern is strictl ...
... Subcellular localization of green fluorescent protein (GFP) – EDE1 fusions on the microtubules of the spindle and spindle poles during mitosis and on the phragmoplast during cytokinesis indicates that EDE1 has a key role in microtubule function during mitosis. Also, its expression pattern is strictl ...
The phosphatidylinositol transfer protein RdgBβ binds 14-3
... ion channel regulation and membrane traffic. PITPs [PI (phosphatidylinositol) transfer proteins] have emerged as key regulators that interface the spatial distribution of PI coupled with its conversion into seven phosphorylated derivatives [1,2]. PITPs are a family of lipid-binding proteins that tra ...
... ion channel regulation and membrane traffic. PITPs [PI (phosphatidylinositol) transfer proteins] have emerged as key regulators that interface the spatial distribution of PI coupled with its conversion into seven phosphorylated derivatives [1,2]. PITPs are a family of lipid-binding proteins that tra ...
1. ELONGATION
... sequences of preferred nucleotides at the 5’ and 3’ ends have been established. In addition to AG, other nucleotides just upstream of the 3 splice junction also are important for precise splicing. Genetica per Scienze Naturali a.a. 05-06 prof S. Presciuttini ...
... sequences of preferred nucleotides at the 5’ and 3’ ends have been established. In addition to AG, other nucleotides just upstream of the 3 splice junction also are important for precise splicing. Genetica per Scienze Naturali a.a. 05-06 prof S. Presciuttini ...
Prolyl Isomerases –Old Proteins as New Therapeutic Targets
... the end of 2012 the total number of publications on cyclophilins since 1990 had surpassed 2,000, a publication with the title “Cyclophilins: Proteins in Search of a Function” provided a good illustration of the still scarce understanding of their biological roles [16]. The early pioneering work by ...
... the end of 2012 the total number of publications on cyclophilins since 1990 had surpassed 2,000, a publication with the title “Cyclophilins: Proteins in Search of a Function” provided a good illustration of the still scarce understanding of their biological roles [16]. The early pioneering work by ...
PDF document
... linked cluster (the šcel’ cluster). The first gene in the cluster encodes the scaffoldin CipC, which is followed by seven genes coding for cellulases, one gene coding for mannanase and one gene coding for rhamnogalacturonan lyase. Three other genes encoding cellulosomal proteins were found to be loc ...
... linked cluster (the šcel’ cluster). The first gene in the cluster encodes the scaffoldin CipC, which is followed by seven genes coding for cellulases, one gene coding for mannanase and one gene coding for rhamnogalacturonan lyase. Three other genes encoding cellulosomal proteins were found to be loc ...
cAMP-Dependent Protein Kinase, Catalytic Subunit Product
... cAMP-Dependent Protein Kinase (PKA) is an ubiquitous serine/threonine protein kinase present in a variety of tissues, including brain, skeletal muscle and heart tissues. Changes in intracellular cAMP levels regulate cellular responses by influencing interaction between the Regulatory (R) and Catalyt ...
... cAMP-Dependent Protein Kinase (PKA) is an ubiquitous serine/threonine protein kinase present in a variety of tissues, including brain, skeletal muscle and heart tissues. Changes in intracellular cAMP levels regulate cellular responses by influencing interaction between the Regulatory (R) and Catalyt ...
A Class of Human Proteins that Deliver Functional
... in the current study possess positively charged amino acids widely dispersed throughout their sequence, and would not have been identified using these previously reported methods. Moreover, the NSHPs we have identified were able to mediate internalization of fused proteins with up to 40-fold higher ...
... in the current study possess positively charged amino acids widely dispersed throughout their sequence, and would not have been identified using these previously reported methods. Moreover, the NSHPs we have identified were able to mediate internalization of fused proteins with up to 40-fold higher ...
Cytochrome P450 Proteins
... Assay selectivity, and sensitivity; Different technical expertise and equipment are needed for mRNA or Western Blotting assessment ...
... Assay selectivity, and sensitivity; Different technical expertise and equipment are needed for mRNA or Western Blotting assessment ...
Protein C-mannosylation: Facts and questions.
... bind (C2-Man)-Trp residues in properdin or terminal complement components, and whether this plays a role in the activation or control of complement. The three-dimensional structure of the TSR or of a protein containing this module has not been determined so far. However, the hydrophilic nature of a ...
... bind (C2-Man)-Trp residues in properdin or terminal complement components, and whether this plays a role in the activation or control of complement. The three-dimensional structure of the TSR or of a protein containing this module has not been determined so far. However, the hydrophilic nature of a ...
Protein replacement therapies
... • In January 1922, Banting and Best used first insulin to treat a 14-years-old patient named Leonard Thomson • He became more ill as the consequence of the injection, but his blood glucose level decreased, therefore the improvement of the preparation technique was decided • 6 weeks later a better ex ...
... • In January 1922, Banting and Best used first insulin to treat a 14-years-old patient named Leonard Thomson • He became more ill as the consequence of the injection, but his blood glucose level decreased, therefore the improvement of the preparation technique was decided • 6 weeks later a better ex ...
BS3 Crosslinking
... Bis (sulfosuccinimidyl) suberate (BS3) crosslinking was performed as described previously (Grosshans et al., 2001, 2002; Conrad et al., 2008). BS3 is a membrane-impermeable agent, which selectively crosslinks cell-surface proteins to form high-molecular-mass aggregates. Because intracellular protein ...
... Bis (sulfosuccinimidyl) suberate (BS3) crosslinking was performed as described previously (Grosshans et al., 2001, 2002; Conrad et al., 2008). BS3 is a membrane-impermeable agent, which selectively crosslinks cell-surface proteins to form high-molecular-mass aggregates. Because intracellular protein ...
C.N.R. Short-term Mobility Program 2008 Gabriella Pocsfalvi
... SSO2749. This protein shows conserved domain of Linocin_M18 bacteriocin protein. Many Grampositive bacteria produce antimicrobial peptides, generally termed bacteriocins. These polypeptides usually has less than 50 amino acid residues long cationic, contain an amphiphilic or hydrophobic region, and ...
... SSO2749. This protein shows conserved domain of Linocin_M18 bacteriocin protein. Many Grampositive bacteria produce antimicrobial peptides, generally termed bacteriocins. These polypeptides usually has less than 50 amino acid residues long cationic, contain an amphiphilic or hydrophobic region, and ...
Lecture 11, chemical genetics - Cal State LA
... Instead of linking drug to a solid support, attach another molecule that is reactive with light or protein functional groups (primary amines) - This “linker” molecule will covalently bind the protein once the drug binds (non-covalently) to its protein target - Linker may be radioactive, so the prote ...
... Instead of linking drug to a solid support, attach another molecule that is reactive with light or protein functional groups (primary amines) - This “linker” molecule will covalently bind the protein once the drug binds (non-covalently) to its protein target - Linker may be radioactive, so the prote ...
Proteolysis in Mixed Organic-Aqueous Solvent
... The resulting peptides are analyzed with matrix-assisted laser desorption ionization (MALDI) mass spectrometry, and this information is interrogated by searching the protein and nucleic acid databases. Although this procedure has become routine, it is inefficient regarding the time required to prepa ...
... The resulting peptides are analyzed with matrix-assisted laser desorption ionization (MALDI) mass spectrometry, and this information is interrogated by searching the protein and nucleic acid databases. Although this procedure has become routine, it is inefficient regarding the time required to prepa ...
The Xanthomonas effector XopJ triggers a conditional
... called type-III effector proteins (T3Es) into their eukaryotic host cell (Galan et al., 2014). These T3Es are targeted to a number of cellular compartments where they influence host cellular processes to provide a beneficial environment for the pathogen to promote pathogen multiplication and disease. I ...
... called type-III effector proteins (T3Es) into their eukaryotic host cell (Galan et al., 2014). These T3Es are targeted to a number of cellular compartments where they influence host cellular processes to provide a beneficial environment for the pathogen to promote pathogen multiplication and disease. I ...
Sample Grant Proposal 2
... vegetative reticulate bodies (RBs). Infection occurs when EBs attach to and are taken up by eukaryotic epithelial cells, a process which has recently been shown to involve local actin cytoskeleton remodeling (7). Within a few hours, they differentiate into RBs and begin to replicate within a membran ...
... vegetative reticulate bodies (RBs). Infection occurs when EBs attach to and are taken up by eukaryotic epithelial cells, a process which has recently been shown to involve local actin cytoskeleton remodeling (7). Within a few hours, they differentiate into RBs and begin to replicate within a membran ...
Fibrous and globular proteins Structure
... elastic fibers is elastin, which is a highly hydrophobic protein and is rich in proline and glycine. It contains some hydroxyproline but no hydroxylysine. It is not glycosylated The primary component, tropoelastin molecules, is cross-linked between lysines to one another. ...
... elastic fibers is elastin, which is a highly hydrophobic protein and is rich in proline and glycine. It contains some hydroxyproline but no hydroxylysine. It is not glycosylated The primary component, tropoelastin molecules, is cross-linked between lysines to one another. ...
Fibrous and globular proteins Structure
... elastic fibers is elastin, which is a highly hydrophobic protein and is rich in proline and glycine. It contains some hydroxyproline but no hydroxylysine. It is not glycosylated The primary component, tropoelastin molecules, is cross-linked between lysines to one another. ...
... elastic fibers is elastin, which is a highly hydrophobic protein and is rich in proline and glycine. It contains some hydroxyproline but no hydroxylysine. It is not glycosylated The primary component, tropoelastin molecules, is cross-linked between lysines to one another. ...
Protein production: feeding the crystallographers and NMR
... Many individual proteins cannot be expressed in soluble form in bacteria. These include one-third to one half of prokaryote proteins (unpublished data). This proportion is likely to be higher for eukaryotic proteins, particularly those that comprise multiple domains, those that require cofactors or ...
... Many individual proteins cannot be expressed in soluble form in bacteria. These include one-third to one half of prokaryote proteins (unpublished data). This proportion is likely to be higher for eukaryotic proteins, particularly those that comprise multiple domains, those that require cofactors or ...
How Do Plant Mitochondria Avoid Importing Chloroplast Proteins
... known sizes of subunits in the yeast complex. In particular, no homologs of Tom37 or Tom22 were apparent and there was an additional protein of around 9 kD. The absence of Tom37 from the plant complex was not so surprising, since this subunit is also missing from the N. crassa complex, and the Tom37 ...
... known sizes of subunits in the yeast complex. In particular, no homologs of Tom37 or Tom22 were apparent and there was an additional protein of around 9 kD. The absence of Tom37 from the plant complex was not so surprising, since this subunit is also missing from the N. crassa complex, and the Tom37 ...
Conjugating Enzymes Belonging to the UbcP4/E2
... key regulatory proteins. The correct progress through the cell cycle is thus under the control of successive events where protein activation alternates with protein degradation mediated by the ubiquitindependent proteolytic pathway. Degradation via this pathway is a two-step process: The protein is ...
... key regulatory proteins. The correct progress through the cell cycle is thus under the control of successive events where protein activation alternates with protein degradation mediated by the ubiquitindependent proteolytic pathway. Degradation via this pathway is a two-step process: The protein is ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.