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Chapter 6: Gene Expression
Chapter 6: Gene Expression

... mRNA Modifications in Eukaryotes: Part I In prokaryotes, transcription and translation can occur simultaneously. In eukaryotes, mRNA must undergo modifications before it crosses the nuclear membrane to the cytoplasm. Once the modified mRNA enters the cytoplasm, it can undergo translation. There are ...
Chromatin Modifications
Chromatin Modifications

... Some HATs have a large but limited region – usually enzymes that are involved in heterochromation formation. No specific HMTs are known to interact with TFs, but some do recruit specifically to coding regions. ...
Chapter 18: Regulation of Gene Expression
Chapter 18: Regulation of Gene Expression

... to affect gene expression. Label the following elements: TATA box, promoter, gene, enhancer, activators, transcription factors, transcription initiation complex, RNA polymerase II, and DNA. Then place your explanation to the right of the figure. EXPLANATION ...
Ch 14- 17 Unit Test - Akron Central Schools
Ch 14- 17 Unit Test - Akron Central Schools

... • Which of the following statements is true about protein synthesis in prokaryotes? • A) Extensive RNA processing is required before prokaryotic transcripts can be translated. • B) Translation can begin while transcription is still in progress. • C) Prokaryotic cells have complicated mechanisms for ...
lecture 20
lecture 20

... Has particular traits and a coding sequence ...
Gene Section ATF2 (activating transcription factor 2) Atlas of Genetics and Cytogenetics
Gene Section ATF2 (activating transcription factor 2) Atlas of Genetics and Cytogenetics

... 5'GTGACGT[AC][AG]-3'). ATF2 binds DNA as a dimer. The specificity of the DNA target sequence that is recognized by dimers containing ATF2 is different depending on whether it is a homodimer or it forms a heterodimer with another JUN protein. ...
Question 1 _____/30 points Question 2 _____/20 points Question 3
Question 1 _____/30 points Question 2 _____/20 points Question 3

... specificity of codon-anticodon pairing. Your first approach is to make a form of EF-Tu that no longer requires the factor binding center of the ribosome to stimulate its GTPase activity. Instead, the mutant EF-Tu hydrolyzes its bound GTP approximately every 20 seconds independent of ribosome binding ...
Using RNA as Molecular Code for Programming Cellular Function
Using RNA as Molecular Code for Programming Cellular Function

... genes. They consist of a ligand-binding domain, the aptamer, which is coupled to an expression platform such that a ligandinduced conformational switch in their structure alters access to a translationally relevant part of the mRNA, like a prokaryotic RBS or a eukaryotic 5′cap, or the production of ...
microarray activity - Blue Valley Schools
microarray activity - Blue Valley Schools

... technology is based on the basic chemistry of DNA. Adenine pairs with thymine and guanine pairs with cytosine. This base complementarity is what allows DNA from cells to bind specifically to known DNA sequences (probes) on a chip. Since a cell expresses hundreds or even thousands of genes at any giv ...
Activity of ribosomes and tmRNA of Streptomyces aureofaciens
Activity of ribosomes and tmRNA of Streptomyces aureofaciens

... GDPNP can substitute GTP in the binding of fMet-tRNA to ribosomes, but increasing concentrations of UTP had no stimulation effect. To investigate whether the stimulatory effect of UTP possibly influences the correct position of fMet-tRNA on ribosome, the transfer of fMet-tRNA from 70S ribosomes to p ...
gene
gene

... • Structural genes: encoding proteins • Regulatory genes: encoding products that interact with other sequences and affect the transcription and translation of these sequences • Regulatory elements: DNA sequences that are not transcribed but play a role in regulating other nucleotide sequences ...
Chapter 3
Chapter 3

... cells, where the DNA can be reproduced in large quantities codon - sequence of three nucleotides in DNA or mRNA that specifies a particular amino acid during protein synthesis; also called triplet. Of the 64 possible codons, three are stop codons, which do not specify amino acids complementary DNA ( ...
Slide 1
Slide 1

... phospholipids that can spontaneously form bilayers and vesicles. ...
Self-Quiz 3 Questions
Self-Quiz 3 Questions

... Some sequences are too long to have only one reading frame Reading frames overlap one another There are often more than one start (methionine) codons in a sequence A codon encompasses 3 nucleotides, one sequence can produce different reading frames depending on which nucleotide is read first How man ...
Structure/function relationship in DNA
Structure/function relationship in DNA

... RNA polymerase recognition of DNA during transcription involves a promoter region and a multi-subunit complex (machine) to conduct a complex process (unwinding DNA, RNA elongation, accurate translation of DNA via proofreading) Numerous proteins involved in regulation of transcription ...
Overcoming the codon bias of E. coli for enhanced protein expression
Overcoming the codon bias of E. coli for enhanced protein expression

... to the demand for one or more tRNAs that may be rare or lacking in the population (3–5). Insufficient tRNA pools can lead to translational stalling, premature translation termination, translation frameshifting and amino acid misincorporation (4). In practice with the pET System and other high-level ...
reading guide
reading guide

... to affect gene expression. Label the following elements: TATA box, promoter, gene, enhancer, activators, transcription factors, transcription initiation complex, RNA polymerase II, and DNA. Then place your explanation to the right of the figure. EXPLANATION ...
Genetics = science of heredity - Suffolk County Community College
Genetics = science of heredity - Suffolk County Community College

... 3. Beginning with an RNA primer complementarily base paired to the single stranded parental DNA, the leading strand is synthesized continuously by the enzyme DNA polymerase in the direction of the replication fork. New tri-phosphate nucleotides from the cytoplasm/nucleoplasm are complementarily base ...
Student Materials - Scope, Sequence, and Coordination
Student Materials - Scope, Sequence, and Coordination

... In the previous activity, you flipped a penny to determine which traits your critter would have. You translated and transcribed the DNA code into messenger RNA and transfer RNA codes. From those codes you determined the amino acid sequence and finally which trait that sequence determined. Now you ar ...
pAmCyan1-N1 Vector Information
pAmCyan1-N1 Vector Information

... base-pair changes, which correspond to human codon-usage preferences, for optimal expression in mammalian cells (2). Additionally, an upstream sequence—located just 5' to the AmCyan1 start codon—has been converted to a Kozak consensus translation initiation site (3) to further increase the translati ...
Genome Organization
Genome Organization

... some originated as tRNA genes and others as 7SL RNA, the RNA involved in the signal recognition particle that guides secreted and membrane protein translation into the endoplasmic reticulum. – Most important SINE is the Alu sequence, which started as a 7SL RNA. Alu sequences make up 7% of genome, ab ...
Review Questions for Ch 1
Review Questions for Ch 1

... adenine (A), guanine (G), and cytosine (C). The fourth base of DNA is thymine (T) whereas the fourth base in RNA is uracil (U). Each DNA molecule consists of two chains of nucleotides forming a double helix held together by hydrogen bonds. RNA consists of a single strand of nucleotides. There is onl ...
Protein Secondary Structure Prediction
Protein Secondary Structure Prediction

... with the help of tRNA. In prokaryotic cells, which have not partition into nucleus and cytoplasm compartments, mRNA can bind to ribosomes while it is being transcribed from DNA. After a certain amount of time the message degrades into its component nucleotides, usually with the assistance of ribonuc ...
Study of Enzyme Mechanisms
Study of Enzyme Mechanisms

... 3-point binding? ...
Manipulating Cells and Viruses in Cultures
Manipulating Cells and Viruses in Cultures

... means to classify viruses. 2. The mRNA that can infect the cell is called the plus strand while the other complementary strand is the negative strand. 3. There are 6 classes due to this system: a. DNA viruses – class I and II – contain DNA Class I has a double strand and class II has a single strand ...
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