PCR settings, pitfalls and artefacts
... • Plasmids work as control templates but can have slightly different conditions compared to gDNA or cDNA • If possible: Certified reference material (cell line documentation) • negative control(s): check for contamination • Run a positive control (a sample known to amplify well) ...
... • Plasmids work as control templates but can have slightly different conditions compared to gDNA or cDNA • If possible: Certified reference material (cell line documentation) • negative control(s): check for contamination • Run a positive control (a sample known to amplify well) ...
Biogenesis of trans-acting siRNAs, endogenous
... generated by miR390 or 22 nt miRNA-containing RISCs is important for subsequent steps. The 5′ and 3′ miR173cleaved fragments accumulate more in the rdr6 mutant than in wild-type, while the 3′ fragments disappear and the 5′ fragments decrease in the sgs3 rdr6 double mutant similar to the sgs3 mutant ...
... generated by miR390 or 22 nt miRNA-containing RISCs is important for subsequent steps. The 5′ and 3′ miR173cleaved fragments accumulate more in the rdr6 mutant than in wild-type, while the 3′ fragments disappear and the 5′ fragments decrease in the sgs3 rdr6 double mutant similar to the sgs3 mutant ...
Transcription and RNA processing
... nascent transcript. The C-terminal domain (CTD) of the largest subunit is then phosphorylated (P) at specific serine residues. Such modification is the signal for binding of the capping enzyme to the CTD and capping (blue) if the nascent RNA. ...
... nascent transcript. The C-terminal domain (CTD) of the largest subunit is then phosphorylated (P) at specific serine residues. Such modification is the signal for binding of the capping enzyme to the CTD and capping (blue) if the nascent RNA. ...
BIO 208 Homework: Bacterial Genetics 2011 17.1 constitutive gene
... 17.1 constitutive gene expression – certain genes are always “on” because the gene products (proteins) are required by the cell all of the time. Regulated gene expression for gene active in response to cellular needs. 17.3 polycistronic mRNA – coding information from more than one gene on one mRNA m ...
... 17.1 constitutive gene expression – certain genes are always “on” because the gene products (proteins) are required by the cell all of the time. Regulated gene expression for gene active in response to cellular needs. 17.3 polycistronic mRNA – coding information from more than one gene on one mRNA m ...
DNA, Proteins, and Biotechnology
... Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA. State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the appl ...
... Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA. State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the appl ...
Slides PPT
... A factor can influence the transcription of a number of genes scattered throughout the genome e.g. CAP. A number of genes which encode catabolic enzymes involved in ...
... A factor can influence the transcription of a number of genes scattered throughout the genome e.g. CAP. A number of genes which encode catabolic enzymes involved in ...
Transcription Regulation Background: Lactose Background: How
... This protein complex binding puts a 90o kink in the DNA and interacts with the alpha subunit of RNA polymerase. Without the cAMP:CAP the lac promoter is a weak promoter varying significantly from the consensus sequence at -10 and -35. The combination of the two controls means beta gal and lac permea ...
... This protein complex binding puts a 90o kink in the DNA and interacts with the alpha subunit of RNA polymerase. Without the cAMP:CAP the lac promoter is a weak promoter varying significantly from the consensus sequence at -10 and -35. The combination of the two controls means beta gal and lac permea ...
tRNA aminoacylation by arginyltRNA synthetase: induced
... conformational changes in the catalytic center of the Ê structure of a protein. The comparison with the 2.9 A binary complex formed by yeast arginyl-tRNA synthetase and tRNAArg reveals that L-arginine binding controls the correct positioning of the CCA end of the tRNAArg. Important structural change ...
... conformational changes in the catalytic center of the Ê structure of a protein. The comparison with the 2.9 A binary complex formed by yeast arginyl-tRNA synthetase and tRNAArg reveals that L-arginine binding controls the correct positioning of the CCA end of the tRNAArg. Important structural change ...
03-131 F 2013 Final Exam Name:_________________________
... 27. Presentation Questions (8 pts). Two are required; two additional can be answered for bonus. Only the first four that you answer will be graded (choose wisely). 1. What is the cause of Huntington’s disease and what is its inheritance pattern? 2. What is the cause of hemophilia and what is its inh ...
... 27. Presentation Questions (8 pts). Two are required; two additional can be answered for bonus. Only the first four that you answer will be graded (choose wisely). 1. What is the cause of Huntington’s disease and what is its inheritance pattern? 2. What is the cause of hemophilia and what is its inh ...
Microarray Services
... • Red spots indicate the genes down-regulated in sample A. • Yellow spots indicate the equal expressions of those genes in sample A and sample B Agilent: two-color gene expression analysis => Not recommended any more ...
... • Red spots indicate the genes down-regulated in sample A. • Yellow spots indicate the equal expressions of those genes in sample A and sample B Agilent: two-color gene expression analysis => Not recommended any more ...
DNA is - Mount Carmel Academy
... Triploid (___) are often ________________ larger and stronger than diploid plants (2N=Normal, 2 sets). ...
... Triploid (___) are often ________________ larger and stronger than diploid plants (2N=Normal, 2 sets). ...
Importance of molecular cell biology investigations in human
... keeps separate most of the genome (= nuclear DNA) as well as other nuclear components (such as some enzymes) separated from cytosolic components. The nucleus is an important example of cellular compartmentalization that allows the cell to properly function. Biochemical reactions are facilitated by t ...
... keeps separate most of the genome (= nuclear DNA) as well as other nuclear components (such as some enzymes) separated from cytosolic components. The nucleus is an important example of cellular compartmentalization that allows the cell to properly function. Biochemical reactions are facilitated by t ...
Primary Sequence of Ovomucoid Messenger RNA as Determined
... IIIA were obtained from Bethesda Research Laboratories, Rockville, Md. Restriction enzymes Hinf I, Hha 1, Hph 1, Mbo II, Psi 1, Kpn I, and Ava 11 were purchased from New England Biolabs, Inc., Beverly, Mass. S, nuclease was obtained from Miles Laboratories, Inc., Elkhart, Ind. Bacterial alkaline pho ...
... IIIA were obtained from Bethesda Research Laboratories, Rockville, Md. Restriction enzymes Hinf I, Hha 1, Hph 1, Mbo II, Psi 1, Kpn I, and Ava 11 were purchased from New England Biolabs, Inc., Beverly, Mass. S, nuclease was obtained from Miles Laboratories, Inc., Elkhart, Ind. Bacterial alkaline pho ...
Supplementary Information (doc 884K)
... Double labeling Immunohistochemistry for D2, NeuN, and GFP in the rat brain Rats were transcardially perfused with 0.1M phosphate-buffered saline (PBS) followed by ice-cold 1% paraformaldehyde (PFA; in 0.1M PBS). Thereafter, brains were removed and left overnight in 1% PFA solution, 2 days in a 20% ...
... Double labeling Immunohistochemistry for D2, NeuN, and GFP in the rat brain Rats were transcardially perfused with 0.1M phosphate-buffered saline (PBS) followed by ice-cold 1% paraformaldehyde (PFA; in 0.1M PBS). Thereafter, brains were removed and left overnight in 1% PFA solution, 2 days in a 20% ...
Fall_Final_Exam_Review
... • The production of chemical compounds by reaction from simpler materials ...
... • The production of chemical compounds by reaction from simpler materials ...
Teacher-submitted assessment ideas
... 4. Let’s think more about the two drawings you just created: How is it possible that mutations in certain locations within PTC gene (nucleotide positions 145, 785 and 886) affect tasting the PTC molecule but mutations in other regions of the PTC gene might not affect tasting PTC? You may answer this ...
... 4. Let’s think more about the two drawings you just created: How is it possible that mutations in certain locations within PTC gene (nucleotide positions 145, 785 and 886) affect tasting the PTC molecule but mutations in other regions of the PTC gene might not affect tasting PTC? You may answer this ...
Chapter 17
... • Three properties of RNA enable it to function as an enzyme – It can form a three-dimensional structure because of its ability to base pair with itself – Some bases in RNA contain functional groups – RNA may hydrogen-bond with other nucleic acid molecules ...
... • Three properties of RNA enable it to function as an enzyme – It can form a three-dimensional structure because of its ability to base pair with itself – Some bases in RNA contain functional groups – RNA may hydrogen-bond with other nucleic acid molecules ...
Untitled
... The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Rapid and very economical method. Does not eliminate genomic DNA. ...
... The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Rapid and very economical method. Does not eliminate genomic DNA. ...
A unifying model for mTORC1-mediated regulation of mRNA translation Please share
... To understand why the translation of TOP and TOP-like mRNAs has a 4E-BP mediated hyper-dependence on mTOR, we considered the established functions of the 4E-BPs3. A key step in eIF4E-dependent initiation is the cooperative binding of eIF4E and eIF4G1 to mRNA, which nucleates the eIF4F complex26. eIF ...
... To understand why the translation of TOP and TOP-like mRNAs has a 4E-BP mediated hyper-dependence on mTOR, we considered the established functions of the 4E-BPs3. A key step in eIF4E-dependent initiation is the cooperative binding of eIF4E and eIF4G1 to mRNA, which nucleates the eIF4F complex26. eIF ...
Chapter 8
... RNA synthesis continues until the polymerase encounters a stop signal. The most common stop signal is a symmetrical inverted repeat of a GCrich sequence followed by seven A ...
... RNA synthesis continues until the polymerase encounters a stop signal. The most common stop signal is a symmetrical inverted repeat of a GCrich sequence followed by seven A ...
Prokaryotic DNA organization • Circular DNA • Condensed by packaging proteins
... – 6 uridines follow hairpin – Requires no accessory proteins ...
... – 6 uridines follow hairpin – Requires no accessory proteins ...
Single molecule analysis - Biomolecular Engineering Laboratory
... Labeling of proteins with two fluorescent dyes (donor and acceptor) Most common conventional method for labeling involves: - Introduction of two cysteine residues into desired sites on proteins Dye heterogeneity Limited to the nucleic acid-interacting proteins and a subset of proteins that a ...
... Labeling of proteins with two fluorescent dyes (donor and acceptor) Most common conventional method for labeling involves: - Introduction of two cysteine residues into desired sites on proteins Dye heterogeneity Limited to the nucleic acid-interacting proteins and a subset of proteins that a ...
August 19, 2002 - People
... • The mRNA in a cell can be isolated and examined using microarrays. Its contents give a snapshot of the genes currently being expressed. • Correlating gene expression with conditions gives hints into the dynamic functioning of the cell. ...
... • The mRNA in a cell can be isolated and examined using microarrays. Its contents give a snapshot of the genes currently being expressed. • Correlating gene expression with conditions gives hints into the dynamic functioning of the cell. ...