Engineering Tyrosine-Based Electron Flow Pathways in Proteins
... radical location on the basis of EPR hyperfine structure. Mutation to tyrosine decreases the lipid peroxidase activity of this myoglobin in the presence of low concentrations of reductant, and the possibility of decreasing the intrinsic toxicity of hemoglobin by introduction of these pathways is disc ...
... radical location on the basis of EPR hyperfine structure. Mutation to tyrosine decreases the lipid peroxidase activity of this myoglobin in the presence of low concentrations of reductant, and the possibility of decreasing the intrinsic toxicity of hemoglobin by introduction of these pathways is disc ...
The Generic Nature of Protein Folding and Misfolding
... protein molecules (Branden and Tooze, 1999). Different proteins are distinguished by a different order of amino acids in the polymeric sequence of typically 300 such building blocks. Following their synthesis in the cell, the majority of proteins must be converted into tightly folded compact structu ...
... protein molecules (Branden and Tooze, 1999). Different proteins are distinguished by a different order of amino acids in the polymeric sequence of typically 300 such building blocks. Following their synthesis in the cell, the majority of proteins must be converted into tightly folded compact structu ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... partment can be compared to the lumen of the endoplasmic reticulum (ER) in eukaryotic cells. Many proteins are found in the periplasm, but the physiological functions of only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the ...
... partment can be compared to the lumen of the endoplasmic reticulum (ER) in eukaryotic cells. Many proteins are found in the periplasm, but the physiological functions of only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the ...
as a PDF
... splice was expressed predominantly in testis. The C2 splice had a more general expression pattern. Therefore, in addition to the distinct features revealed by amino acid sequences, these splice variants also were expressed in a tissue-specific manner. Because the N0 splice contained sequences shared ...
... splice was expressed predominantly in testis. The C2 splice had a more general expression pattern. Therefore, in addition to the distinct features revealed by amino acid sequences, these splice variants also were expressed in a tissue-specific manner. Because the N0 splice contained sequences shared ...
669 Salmonella typhimurium - Journal of General Virology
... Immediately after binding, the tail proteins begin to cleave the O antigen. As the rhamnosylgalactose linkages are cleaved, the proteins apparently migrate down the chain. This is based on the fact that when unattached tail protein is bound to ceils, it is not found free in the medium until some tim ...
... Immediately after binding, the tail proteins begin to cleave the O antigen. As the rhamnosylgalactose linkages are cleaved, the proteins apparently migrate down the chain. This is based on the fact that when unattached tail protein is bound to ceils, it is not found free in the medium until some tim ...
Association of CAD, a multifunctional protein involved in pyrimidine
... FLAG-mLST8 vector and Myc-tagged CAD expression vector of either the full length or each deletion mutant, and the cell extracts were subjected to immunoprecipitation with the anti-myc antibody. The extracts and immunoprecipitates were analyzed by immunoblot with the antimyc or anti-FLAG antibody. Th ...
... FLAG-mLST8 vector and Myc-tagged CAD expression vector of either the full length or each deletion mutant, and the cell extracts were subjected to immunoprecipitation with the anti-myc antibody. The extracts and immunoprecipitates were analyzed by immunoblot with the antimyc or anti-FLAG antibody. Th ...
Do Legume Storage Proteins Play a Role in
... Departamento de Bioquı́mica, Universidade Federal do Rio Grande do Norte, 59072–970 Natal, Rio Grande do Norte, Brazil (M.P.S.); Departamento de Biologia Celular, Universidade de Brası́lia, Brasilia, Distrito Federal, Brazil (I.R.G.); Embrapa-Cenargen, Setor de Áreas Isoladas Norte Parque Rural, 70 ...
... Departamento de Bioquı́mica, Universidade Federal do Rio Grande do Norte, 59072–970 Natal, Rio Grande do Norte, Brazil (M.P.S.); Departamento de Biologia Celular, Universidade de Brası́lia, Brasilia, Distrito Federal, Brazil (I.R.G.); Embrapa-Cenargen, Setor de Áreas Isoladas Norte Parque Rural, 70 ...
Bio2 Gene prediction DNA structure Codons and ORFs Predicting
... of this approach: • HMMs can be visualised as finite state machines with a begin and an end state. • FSMs move through a series of state emitting some kind of output report either at the end or during a transition from one state to another. ...
... of this approach: • HMMs can be visualised as finite state machines with a begin and an end state. • FSMs move through a series of state emitting some kind of output report either at the end or during a transition from one state to another. ...
The Caenorhabditis elegans mRNA 5`
... consists of an amino (N)-terminal region with RTPase activity and a carboxy (C)-terminal region that resembles known GTases, However, CEL-1 has not previously been shown to have GTase activity. Cloning of the CEL-1 cDNA shows that the full-length protein has 623 amino acids, including an additional ...
... consists of an amino (N)-terminal region with RTPase activity and a carboxy (C)-terminal region that resembles known GTases, However, CEL-1 has not previously been shown to have GTase activity. Cloning of the CEL-1 cDNA shows that the full-length protein has 623 amino acids, including an additional ...
occasional article intracellular protein degradation: from a vague
... when needed. The lysosomal membrane provided this fencing mechanism. Obviously, nobody could have predicted that a new mode of post-translational modification -ubiquitination- could function as a proteolysis signal, and that untagged proteins will remain protected. Thus, while the structure of the l ...
... when needed. The lysosomal membrane provided this fencing mechanism. Obviously, nobody could have predicted that a new mode of post-translational modification -ubiquitination- could function as a proteolysis signal, and that untagged proteins will remain protected. Thus, while the structure of the l ...
The plastid division proteins, FtsZ1 and FtsZ2, differ in their
... thaliana and by a gene family of at least four members in Nicotiana tabacum (an allotetraploid species). In both plants, a small multigene family encodes FtsZ2, with two members in Arabidopsis thaliana. It was demonstrated recently that the classification of FtsZ proteins into two distinct groups al ...
... thaliana and by a gene family of at least four members in Nicotiana tabacum (an allotetraploid species). In both plants, a small multigene family encodes FtsZ2, with two members in Arabidopsis thaliana. It was demonstrated recently that the classification of FtsZ proteins into two distinct groups al ...
Function of Conserved Tryptophans in the Aspergillus niger
... W590K mutation resulting in the abolition of binding at site 1 since W543 and W590 are both key binding residues at this site. By contrast, signals associated with binding site 2 shift by similar amounts in wild-type SBD and W590K (results not shown). Comparable results were obtained from W563K, sho ...
... W590K mutation resulting in the abolition of binding at site 1 since W543 and W590 are both key binding residues at this site. By contrast, signals associated with binding site 2 shift by similar amounts in wild-type SBD and W590K (results not shown). Comparable results were obtained from W563K, sho ...
Highly conserved epitope domain in major core protein p24 is
... homology not only between HIV-1 and SIVAGM, but also between HIV-1 and FIV (Fig. 3), implying that the region might be important for this protein to exhibit its essential function. In the epitope regions consisting of 15 amino acids, although one amino acid change between HIV-1 and SIVAGM and nine c ...
... homology not only between HIV-1 and SIVAGM, but also between HIV-1 and FIV (Fig. 3), implying that the region might be important for this protein to exhibit its essential function. In the epitope regions consisting of 15 amino acids, although one amino acid change between HIV-1 and SIVAGM and nine c ...
Lethal protein produced in response to competition
... of the proteins on P. dendritiformis colony growth, various concentrations of subtilisin were placed near growing colonies (Fig. 3A). Low subtilisin levels (Fig. 3A, Upper Line) promoted bacterial reproduction and colony expansion, whereas higher subtilisin levels (Fig. 3A, Middle Line) initially pr ...
... of the proteins on P. dendritiformis colony growth, various concentrations of subtilisin were placed near growing colonies (Fig. 3A). Low subtilisin levels (Fig. 3A, Upper Line) promoted bacterial reproduction and colony expansion, whereas higher subtilisin levels (Fig. 3A, Middle Line) initially pr ...
AHM1, a Novel Type of Nuclear Matrix–Localized
... in Figure 2A) (Kelley, 1998; Mayer and Bukau, 1998). As evidenced from the alignment shown in Figure 2A, the J domain–like region of AHM1 has considerably diverged from the canonical J domains of Escherichia coli DnaJ and its close relatives. However, the divergence is similar to the difference seen ...
... in Figure 2A) (Kelley, 1998; Mayer and Bukau, 1998). As evidenced from the alignment shown in Figure 2A, the J domain–like region of AHM1 has considerably diverged from the canonical J domains of Escherichia coli DnaJ and its close relatives. However, the divergence is similar to the difference seen ...
found that these mutants differed greatly
... found that these mutants differed greatly in the activity of the phenylalanine (and p-fluorophenylalanine) permwre. They ranged in activity from as low 01 20% to greater than 100% when compared to the control (ST74A). Crosses between these mutants hove indicated that there are at least 2 and possibl ...
... found that these mutants differed greatly in the activity of the phenylalanine (and p-fluorophenylalanine) permwre. They ranged in activity from as low 01 20% to greater than 100% when compared to the control (ST74A). Crosses between these mutants hove indicated that there are at least 2 and possibl ...
Intracellular Protein Degradation
... mechanism of entry of cytosolic proteins into the lysosomal lumen, major problems have remained unsolved. Important among them were: (i) the varying half-lives, (ii) the energy requirement, and (iii) the distinct response of different populations of proteins to lysosomal inhibitors. Thus, according ...
... mechanism of entry of cytosolic proteins into the lysosomal lumen, major problems have remained unsolved. Important among them were: (i) the varying half-lives, (ii) the energy requirement, and (iii) the distinct response of different populations of proteins to lysosomal inhibitors. Thus, according ...
FREE Sample Here
... requirement of the individual. Increasing or decreasing one energy source while consuming a set amount of kilocalories affects the intake of the other sources of energy. a. Both the statement and the reason are correct and related. b. Both the statement and the reason are correct but are not related ...
... requirement of the individual. Increasing or decreasing one energy source while consuming a set amount of kilocalories affects the intake of the other sources of energy. a. Both the statement and the reason are correct and related. b. Both the statement and the reason are correct but are not related ...
Deficient Saccharomyces cerevisiae and on the
... of intracellular amino acids and acid-labile phosphate in the ycast. The DNA chontent of ycast grown in adcniri e-containing biotin-deficient media remained UII changed. Formation of pink 1)igment was suppressed in yeast grown in media containing more than 0.25 x 10 -"Rr-adenine (Chamberlain & Rainb ...
... of intracellular amino acids and acid-labile phosphate in the ycast. The DNA chontent of ycast grown in adcniri e-containing biotin-deficient media remained UII changed. Formation of pink 1)igment was suppressed in yeast grown in media containing more than 0.25 x 10 -"Rr-adenine (Chamberlain & Rainb ...
Computer Storage of Sequences
... Comprehensive and expertly annotated protein sequence database. The primary sources of PSD data are sequences from GenBank/EMBL/DDBJ translations, published literature, and direct submission to PIR-International. ...
... Comprehensive and expertly annotated protein sequence database. The primary sources of PSD data are sequences from GenBank/EMBL/DDBJ translations, published literature, and direct submission to PIR-International. ...
A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with
... has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot ...
... has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot ...
29. protein targeting and degradation
... bulge out from donor compartments and fuse with target compartments. The signals used to target eukaryotic proteins for transfer across the ER membrane are ancient, for bacteria also use similar sequences or signals for sending proteins to their plasma membrane and to secrete them. The transported p ...
... bulge out from donor compartments and fuse with target compartments. The signals used to target eukaryotic proteins for transfer across the ER membrane are ancient, for bacteria also use similar sequences or signals for sending proteins to their plasma membrane and to secrete them. The transported p ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.