When epigenetics meets alternative splicing: the roles of DNA
... contain higher levels of DNA methylation than noncoding regions. So the question remains: do high levels of DNA methylation on exons occur circumstantially, as an outcome of high exonic GC content, or does DNA methylation have a biological function in the exon selection process? To answer this quest ...
... contain higher levels of DNA methylation than noncoding regions. So the question remains: do high levels of DNA methylation on exons occur circumstantially, as an outcome of high exonic GC content, or does DNA methylation have a biological function in the exon selection process? To answer this quest ...
Update on Carbapenem Resistant Enterobacteriaceae
... • Enterobacteriaceae are gram-negative bacteria that can cause invasive disease but generally have been susceptible to a variety of antibiotics. • Carbapenem-Resistant Enterobacteriaceae (CRE) are Enterobacteriaceae that have become highly resistant to most or all antibiotics through several mechani ...
... • Enterobacteriaceae are gram-negative bacteria that can cause invasive disease but generally have been susceptible to a variety of antibiotics. • Carbapenem-Resistant Enterobacteriaceae (CRE) are Enterobacteriaceae that have become highly resistant to most or all antibiotics through several mechani ...
Exam 2 Answer Key Spring 1996 Mcbio 316 - page 1
... As described above FO-1 and FO-3 complement each other, so every cell coinfected with both phage will be lysed. In contrast, the results indcate that FO-1 and FO-2 cannot complement each other, so the rare plaques observed are probably due to recombination between the two phage to produce wild-type ...
... As described above FO-1 and FO-3 complement each other, so every cell coinfected with both phage will be lysed. In contrast, the results indcate that FO-1 and FO-2 cannot complement each other, so the rare plaques observed are probably due to recombination between the two phage to produce wild-type ...
Virus on virus infects bacterium
... and the replicative form of its DNA is not very abundant. These properties may be related to the many functions of the genes that it encodes, or they may reflect a regulatory mechanism that balances development of the phage with formation of the pilus. Another amazing link between the VPIF and CTXF ...
... and the replicative form of its DNA is not very abundant. These properties may be related to the many functions of the genes that it encodes, or they may reflect a regulatory mechanism that balances development of the phage with formation of the pilus. Another amazing link between the VPIF and CTXF ...
Chapter 4 powerpoint file
... occur. They may also perform reactions that would not otherwise take place. ...
... occur. They may also perform reactions that would not otherwise take place. ...
selection for recombination in small populations
... this result (see also Fig. 1). Negative epistasis generates negative linkage disequilibria among favorable alleles, such that beneficial alleles tend to be found on genetic backgrounds that are less fit than average (Eshel and Feldman 1970). With negative disequilibria among favorable alleles, recom ...
... this result (see also Fig. 1). Negative epistasis generates negative linkage disequilibria among favorable alleles, such that beneficial alleles tend to be found on genetic backgrounds that are less fit than average (Eshel and Feldman 1970). With negative disequilibria among favorable alleles, recom ...
THE LOD SCORE METHOD
... Again, nail-patella syndrome appears to be dominant because affected individuals all had at least one parent with the disease. From an examination of the progeny in generation II, it is possible to determine the complete genotypes of the parents in generation I. The non-affected parent must be homoz ...
... Again, nail-patella syndrome appears to be dominant because affected individuals all had at least one parent with the disease. From an examination of the progeny in generation II, it is possible to determine the complete genotypes of the parents in generation I. The non-affected parent must be homoz ...
Practice exam 3 key
... pUC map (4 pts) -1 pt for each error in site or fragment length; 1 pt if just the 3 sites shown IF relative spacing approximately correct. Plasmids X and Y (8 pts): vector fragments correct (3 pts); yfg/EcoRI fragment = 1 kb (1 pt); BamHI site subdivides yfg/EcoRI fragment into 0.2 kb and 0.8 kb (2 ...
... pUC map (4 pts) -1 pt for each error in site or fragment length; 1 pt if just the 3 sites shown IF relative spacing approximately correct. Plasmids X and Y (8 pts): vector fragments correct (3 pts); yfg/EcoRI fragment = 1 kb (1 pt); BamHI site subdivides yfg/EcoRI fragment into 0.2 kb and 0.8 kb (2 ...
Yeast as a Model Genetic Organism
... An important theme of yeast biology is the use and study of homologous recombination, a process by which a broken piece of DNA uses a homologous DNA template as a substrate for repair. Yeast use this process to fix DNA damage, to switch mating types, and to segregate homologous chromosomes during mei ...
... An important theme of yeast biology is the use and study of homologous recombination, a process by which a broken piece of DNA uses a homologous DNA template as a substrate for repair. Yeast use this process to fix DNA damage, to switch mating types, and to segregate homologous chromosomes during mei ...
1 X chromosome crossover formation and genome stability in
... xnd-1(ok709); LG IV, ced-3(n717); LG V, mys-1(n3681), him-5(ok1896). Some strains were provided by the Caenorhabditis Genetics Center. xnd-1(ok709) was outcrossed multiple times for these studies due to long-term maintenance problems of the strain. Double and triple mutants were generated using sta ...
... xnd-1(ok709); LG IV, ced-3(n717); LG V, mys-1(n3681), him-5(ok1896). Some strains were provided by the Caenorhabditis Genetics Center. xnd-1(ok709) was outcrossed multiple times for these studies due to long-term maintenance problems of the strain. Double and triple mutants were generated using sta ...
towards synthetic plant genomes, transcriptomes and epigenomes
... derived from pathogens from previous infection flanked by short repeats. After transcription of a long RNA, short individual RNA molecules (crRNA, abbreviation for CRISPR RNA) are processed and set free. Together with a second conserved RNA (tracRNA, abbreviation for tracing RNA), they define the sp ...
... derived from pathogens from previous infection flanked by short repeats. After transcription of a long RNA, short individual RNA molecules (crRNA, abbreviation for CRISPR RNA) are processed and set free. Together with a second conserved RNA (tracRNA, abbreviation for tracing RNA), they define the sp ...
Next-Generation Sequencing applied to aDNA
... sumatran_orangutan/ This folder contains trimmed reads (organized as in the makefile), and other intermediate files; these are often useful in other analyses, but may be deleted to save space. Next, genotype the coding genes on the mitochondrial genome and build a phylogeny using the following comma ...
... sumatran_orangutan/ This folder contains trimmed reads (organized as in the makefile), and other intermediate files; these are often useful in other analyses, but may be deleted to save space. Next, genotype the coding genes on the mitochondrial genome and build a phylogeny using the following comma ...
Genome Sequence Quality - Rice Genome Annotation Project
... – Gaps may exist and can be of an unknown size – In some cases, the complete sequence of the BAC may exist but the quality scores are too low for some regions to promote the record to phase 3 ...
... – Gaps may exist and can be of an unknown size – In some cases, the complete sequence of the BAC may exist but the quality scores are too low for some regions to promote the record to phase 3 ...
Towards Programmable Molecular Machines
... Tian et al. [10] also proposed a walker using DNAzymes. In this construction, the walker is a DNAzyme. It attaches to single stranded DNAs anchored on the substrate, makes a cut and moves onto the next DNA strand. Pei et al. [4] extended the DNAzyme walker to a 2-dimensional walker. In their design, ...
... Tian et al. [10] also proposed a walker using DNAzymes. In this construction, the walker is a DNAzyme. It attaches to single stranded DNAs anchored on the substrate, makes a cut and moves onto the next DNA strand. Pei et al. [4] extended the DNAzyme walker to a 2-dimensional walker. In their design, ...
Introduction of Microarray
... Basic DNA detection technique that has been used for over 30 years, known as Southern blots: ...
... Basic DNA detection technique that has been used for over 30 years, known as Southern blots: ...
Ultramer® Oligonucleotides - Integrated DNA Technologies
... of Ultramer Oligonucleotides for de novo gene construction. Many of the published procedures for constructing specific DNA sequences from oligos use relatively short sequences (≤60mer) with overlapping stretches of common sequence that permit annealing, followed by assembly using PCR-based strategie ...
... of Ultramer Oligonucleotides for de novo gene construction. Many of the published procedures for constructing specific DNA sequences from oligos use relatively short sequences (≤60mer) with overlapping stretches of common sequence that permit annealing, followed by assembly using PCR-based strategie ...
Prentice Hall Biology - Mid
... amounts of DNA called repeats that do not code for proteins. This DNA varies from person to person. Here, one sample has 12 repeats between genes A and B, while the second sample has 9 repeats. ...
... amounts of DNA called repeats that do not code for proteins. This DNA varies from person to person. Here, one sample has 12 repeats between genes A and B, while the second sample has 9 repeats. ...
1 BIOL2323: GENERAL GENETICS STUDY GUIDE
... know the basic genetic terminology: gene, allele, locus, phenotype, genotype, wild type, homozygous, heterozygous, recessive, dominant know symbols used in pedigree analysis determine if a disease is dominant, recessive, autosomal, or X-chromosomal using pedigree analysis explain what multifactorial ...
... know the basic genetic terminology: gene, allele, locus, phenotype, genotype, wild type, homozygous, heterozygous, recessive, dominant know symbols used in pedigree analysis determine if a disease is dominant, recessive, autosomal, or X-chromosomal using pedigree analysis explain what multifactorial ...
Microbiology
... Cloning and sequencing of the eg/ and bgl genes. Clones harbouring the bglS and eglS genes were isolated from a genomic library of B. subtilis 168 cloned in phage A L47.1 (Putzer e t al., 1990). DNA from phage clones exhibited P-glucan hydrolysing activity was isolated, digested by Hind111 and subcl ...
... Cloning and sequencing of the eg/ and bgl genes. Clones harbouring the bglS and eglS genes were isolated from a genomic library of B. subtilis 168 cloned in phage A L47.1 (Putzer e t al., 1990). DNA from phage clones exhibited P-glucan hydrolysing activity was isolated, digested by Hind111 and subcl ...
PopGen2: Linkage Disequilibrium
... An alternative to the family study is a population based approach called LINKAGE DISEQUILIBRIUM MAPPING (or LD MAPPING), and sometimes called an ALLELIC ASSOCIATION STUDY, and it is applied to a population rather than a family pedigree. LD mapping is based on the fact that the mutation in the gene t ...
... An alternative to the family study is a population based approach called LINKAGE DISEQUILIBRIUM MAPPING (or LD MAPPING), and sometimes called an ALLELIC ASSOCIATION STUDY, and it is applied to a population rather than a family pedigree. LD mapping is based on the fact that the mutation in the gene t ...
File
... The photograph shows Rainbow and CC (CC is Rainbow’s clone). Why is CC’s coat pattern different from Rainbow’s given that CC and Rainbow are genetically identical? a) random X chromosome inactivation b) heterozygous at coat color gene locus c) environmental effects on gene expression d) all of the ...
... The photograph shows Rainbow and CC (CC is Rainbow’s clone). Why is CC’s coat pattern different from Rainbow’s given that CC and Rainbow are genetically identical? a) random X chromosome inactivation b) heterozygous at coat color gene locus c) environmental effects on gene expression d) all of the ...
Directions for Use Taq DNA Polymerase, 500U
... (ung-) by a modified ung gene derived from Atlantic Cod. It degrades uracil-containing singleand double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolyzing the Nglycosidic bond between deoxyribose sugar and the base in uracil. This generates alkalinesensitive apyramidinic sites in ...
... (ung-) by a modified ung gene derived from Atlantic Cod. It degrades uracil-containing singleand double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolyzing the Nglycosidic bond between deoxyribose sugar and the base in uracil. This generates alkalinesensitive apyramidinic sites in ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.