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CH24
CH24

... --There are three primary sources of genetic variation Mutations are changes in the DNA structure Gene flow in genes’ movement [Sex determination (non somatic cells)] --It provides the raw material for natural selection ...
Bacteria
Bacteria

... • Beneficial mutations can accumulate rapidly in a population, allowing for rapid evolution • ex. Antibiotic resistant strains are becoming more common ...
PDF
PDF

... by their promoter region located upstream of the coding regions (e.g. Hampsey, 1998). There are ∼200 known yeast transcription factors including activators/repressors and coactivators/co-repressors, in addition to basal factors. The availability of the complete genome enables largescale functional s ...
Note 20 - South Tuen Mun Government Secondary School
Note 20 - South Tuen Mun Government Secondary School

... Mendel’s first law / Law of segregation – a character is controlled by a pair of alleles, the alleles separate during gamete formation and recombines randomly in fertilization. Phenotype – the observable character Genotype – the genetic make up Homozygous – the two alleles of a gene is the same e.g. ...
Chapter 13 Lecture Notes: DNA Function I. Transcription (General
Chapter 13 Lecture Notes: DNA Function I. Transcription (General

... a) Can bind to specific DNA sequences and help RNA polymerase initiate transcription via protein-protein interactions or by altering the structure of the DNA. b) Transcription of some promoters requires an accessory transcriptional activator; at other promoters, the activators just increase the rate ...
Lecture Outline
Lecture Outline

... hydrocarbons: found in smoke of all kinds) Deaminating agents affects cytosine and adenine nitrous acid Oxidative reactions reactive forms of oxygen produced by normal aerobic metabolism superoxide radicals, hydrogen peroxide, hydroxyl radicals Intercalating agents ethidium bromide agents insert bet ...
light - Microbiology
light - Microbiology

... bacterial chromosome, during the repair of U.V. damage, facilitates pairing with homologous regions of the complementary sex factor strand. A recombination event, mediated by breakage and covalent bonding, then joins a free end of the excised DNA strand to the paired sex factor strand. In this way, ...
To learn how to use a spectrophotometer. UV/visible spectrophotometer Quartz Cuvette
To learn how to use a spectrophotometer. UV/visible spectrophotometer Quartz Cuvette

... Tissue and cells are composed of a variety of organic compounds (DNA, proteins, lipids, and carbohydrates). Light interacts with these molecules in different ways: reflection, absorption, transmission, and scattering. All biophotonic applications involve a light source that is passed through a targ ...
Ultraviolet Induction of Chromosome Transfer by
Ultraviolet Induction of Chromosome Transfer by

... bacterial chromosome, during the repair of U.V. damage, facilitates pairing with homologous regions of the complementary sex factor strand. A recombination event, mediated by breakage and covalent bonding, then joins a free end of the excised DNA strand to the paired sex factor strand. In this way, ...
Chem 465 Biochemistry II Hour Exam 3
Chem 465 Biochemistry II Hour Exam 3

... Has a large CTD (carboxyterminal domain) that has several specialized functions not seen in Ecoli like sites to bind capping and splicing complexes. Will also have lots of interactions with other proteins as part of the more complex control mechanisms seen n eukaryotes. ...
Figure 2: Construction of a gene deletion using BRED.
Figure 2: Construction of a gene deletion using BRED.

... mycobacteriophages that we have termed BRED: Bacteriophage recombineering with electroporated DNA. This method utilizes recombineering-proficient strains of Mycobacterium smegmatis that have elevated recombination frequencies due to the expression of phage-derived proteins. It is described in furthe ...
A 2 - Computer Science
A 2 - Computer Science

... LocusLink is a great starting point: it collects key information on each gene/protein from major databases. It now covers 8 organisms. RefSeq provides a curated, optimal accession number for each DNA (NM_006744) or protein (NP_007635) [2] Entrez [3] UniGene [4] ExPASy SRS ...
Question Marking Guidance Mark Comments 01.1 1. Equilibrium
Question Marking Guidance Mark Comments 01.1 1. Equilibrium

... 35–36 (μmol O2 mg–1);; ...
as a PDF
as a PDF

... over the length of the gene. A cycle of 6 steps is then postulated for the matching of each slave in turn against the master, namely: (1) breakage of the complementary chain of the slave at the terminus (non-operator) end of the gene; (2) dissociation of the chains of the slave over the length of th ...
Name - the BIOTECH Project
Name - the BIOTECH Project

... shelter. Also, the plates were stored at 37°C, which is their favorite temperature. 2. If the bacteria can grow on LB agar so well, why didn't they grow on LB agar with ampicillin? This is an opportunity to talk about what ampicillin is. The bacteria aren't growing on LB agar with ampicillin because ...
Keynote for 2008 Genomics Workshop
Keynote for 2008 Genomics Workshop

... in leaves of sweet wormwood ...
2 Marks
2 Marks

... List five natural plant growth regulators. Write a note on discovery, physiological functions and agricultural/horticultural applications of any one of them. ...
Urania basin brine Bannock basin interface l`Atalante basin interface
Urania basin brine Bannock basin interface l`Atalante basin interface

... Fragment size (base pairs) ...
DNA and RNA: Composition and Structure
DNA and RNA: Composition and Structure

... • Denaturation or inhibition may change protein structure - will change its function • Coenzyme and co factor may enhance the protein’s structure ...
1 of 1 Study Questions for Topic 7: Linkage Analysis in Mice and
1 of 1 Study Questions for Topic 7: Linkage Analysis in Mice and

... twe en -I- -I- -I- and x y z produced an ascus with the following ordered array of ascospores (only one member of each spore pair is shown): (-I--I- z) (+ Y z) (x + +) (x y +). (a) Is this ascus most likely the result of a meiotic event in which 0, 1, 2, or 3 crossovers occurred? (b) In what intcr ...
TRaNsgeNIC faRm aNImal pRODUCTION aND
TRaNsgeNIC faRm aNImal pRODUCTION aND

... animal physiology and/or anatomy; cloning procedures to reproduce specific blood lines; and h) developing animals specially created for use in xenografting. The different animal systems to produce recombinant proteins 1) Milk: Milk is currently the best available bioreactor. Extensive studies have ...
RNA Metabolism Summary Slides as Questions
RNA Metabolism Summary Slides as Questions

... eukaryotes, can be a few minutes or up to a day. Without degradation, the mRNA concentraion would be out of control.**Bacterial mRNA is degraded by an endoribonuclease followed by 3' to 5' exoribonuclease action. 19. What happens when the Poly A tail is shortened to less than 25 A's? In which direct ...
Plasmid Sex Introduction .....In most bacteria there are several
Plasmid Sex Introduction .....In most bacteria there are several

... Transformation is another method of acquiring resistance. During transformation, bacterial cells take up DNA from the surrounding environment. Certain requirements exist in order for transformation to take place. First, exogenous DNA must be present in the immediate environment. Bacteria must have m ...
Adaptive value of sex in microbial pathogens
Adaptive value of sex in microbial pathogens

... There may be other effects, indeed, with a trait like sex which is so entrenched in the biology of life we expect there to be a variety of other significant effects. The reduction of genetical associations between alleles that can result from recombination is certainly among these significant effect ...
Methods, Applications and Policy for Agriculture OVERVIEW
Methods, Applications and Policy for Agriculture OVERVIEW

... • Canada takes a product-based approach to assessing plants with novel traits (PNTs) for use as food, as feed, and for release into the Canadian environment. − The trigger for regulation in all cases is based on novelty. − The regulatory trigger is not identical for novel foods, novel feeds, and PNT ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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