![Protein Denaturation Studies Using the Pyris 6 DSC](http://s1.studyres.com/store/data/022106979_1-7bbce6d5a23359697078f57367f96f61-300x300.png)
Protein Denaturation Studies Using the Pyris 6 DSC
... instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional structures that allows them to support specific biological functions. When heat is applied to the protein, this shap ...
... instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional structures that allows them to support specific biological functions. When heat is applied to the protein, this shap ...
Testis-specific TAF homologs collaborate to control a
... proteins that might collaborate with the dTAF5 homolog can to regulate expression of specific target genes in Drosophila spermatocytes, we investigated the expression and function of other TAFII homologs in the Drosophila genome. We show that Drosophila primary spermatocytes express several addition ...
... proteins that might collaborate with the dTAF5 homolog can to regulate expression of specific target genes in Drosophila spermatocytes, we investigated the expression and function of other TAFII homologs in the Drosophila genome. We show that Drosophila primary spermatocytes express several addition ...
Gene Section CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1)
... 7alpha-hydroxylase deficiency exhibit, however, different phenotypes (Norlin and Wikvall, 2007). Several mechanisms for regulation of CYP7A1 have been described (Chiang, 2004). Bile acids inhibit CYP7A1 gene transcription via negative feedback control. The feedback inhibition by bile acids involve s ...
... 7alpha-hydroxylase deficiency exhibit, however, different phenotypes (Norlin and Wikvall, 2007). Several mechanisms for regulation of CYP7A1 have been described (Chiang, 2004). Bile acids inhibit CYP7A1 gene transcription via negative feedback control. The feedback inhibition by bile acids involve s ...
Examination test of Proteins The repeating units of proteins are
... a) Alfa-amylase, hexokinase, lactase b) Maltase, sucrase, enterokinase c) * Lactase, alfa-amylase, sucrase d) Sucrase, maltase, glycogen phosphorylase 31. The sugar which forms major component of nucleic acids is a) *ribose b) galactose c) mannose d) maltose 32. Under aerobic condition pyruvate is c ...
... a) Alfa-amylase, hexokinase, lactase b) Maltase, sucrase, enterokinase c) * Lactase, alfa-amylase, sucrase d) Sucrase, maltase, glycogen phosphorylase 31. The sugar which forms major component of nucleic acids is a) *ribose b) galactose c) mannose d) maltose 32. Under aerobic condition pyruvate is c ...
University of Groningen Structure and mechanism of the ECF
... work in the opposite direction. In contrast to the well-conserved NBDs, the TMDs are polyphyletic (144) and can thus adopt very different folds (see figure 2). Prokaryotic ABC transporters There is a wealth of biochemical (and to a lesser extent) structural data available on prokaryotic ABC transpor ...
... work in the opposite direction. In contrast to the well-conserved NBDs, the TMDs are polyphyletic (144) and can thus adopt very different folds (see figure 2). Prokaryotic ABC transporters There is a wealth of biochemical (and to a lesser extent) structural data available on prokaryotic ABC transpor ...
1 Nucleoli: Composition, Function and Dynamics
... localization of pre-miRNAs and Dicer-like 1 (DCL1) to D-bodies also suggests a role for the nucleolus in the maturation of miRNAs (Pontes and Pikaard, 2008). Some precursor and mature miRNAs are enriched in the nucleolus of mammalian cells possibly for modification, assembly or to regulate snoRNA ac ...
... localization of pre-miRNAs and Dicer-like 1 (DCL1) to D-bodies also suggests a role for the nucleolus in the maturation of miRNAs (Pontes and Pikaard, 2008). Some precursor and mature miRNAs are enriched in the nucleolus of mammalian cells possibly for modification, assembly or to regulate snoRNA ac ...
Post-translational Modifications
... Composed of oligosaccharides and inositol phospholipids Provides a mechanism for anchoring cell-surface proteins to the membrane as a flexible leash that allows the entire protein (except for anchor) to be in extracellular space (unlike a transmembrane protein) Added to translocated proteins in E ...
... Composed of oligosaccharides and inositol phospholipids Provides a mechanism for anchoring cell-surface proteins to the membrane as a flexible leash that allows the entire protein (except for anchor) to be in extracellular space (unlike a transmembrane protein) Added to translocated proteins in E ...
Protein Structure Prediction
... selected from PDB and other databases such as PSST (http://pranag.physics.iisc.ernet.in/psst) and PRF (http://www.genome.ad.jp/) have been selected. • The data set has been restricted to those biologically active peptides that consist of only natural amino acids and are linear with length varying be ...
... selected from PDB and other databases such as PSST (http://pranag.physics.iisc.ernet.in/psst) and PRF (http://www.genome.ad.jp/) have been selected. • The data set has been restricted to those biologically active peptides that consist of only natural amino acids and are linear with length varying be ...
Calcitonin
... Calcitonin is a 32-amino acid polypeptide hormone that is produced in humans primarily by the parafollicular (also known as C-cells) of the thyroid, and in many other animals in the ultimobranchia body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone(PTH). It has ...
... Calcitonin is a 32-amino acid polypeptide hormone that is produced in humans primarily by the parafollicular (also known as C-cells) of the thyroid, and in many other animals in the ultimobranchia body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone(PTH). It has ...
Molecular and Immunological Methods
... temperatures were needed to denature the double stranded DNA produced, and allow more DNA replication. The discovery of a thermostable DNA polymerase in Thermus aquaticus allowed it’s inclusion in a series of repeated thermal cycles, in which the DNA was denatured to single strands, the primers an ...
... temperatures were needed to denature the double stranded DNA produced, and allow more DNA replication. The discovery of a thermostable DNA polymerase in Thermus aquaticus allowed it’s inclusion in a series of repeated thermal cycles, in which the DNA was denatured to single strands, the primers an ...
Protein prediction methods – steps of analysis
... cytoplasm, in the lumen of an organelle or in the extracellular environments and those that are membrane attached associated wit a lipid bilayer. They can be integral membrane proteins or they may be peripherally attached to membranes. Proteins are also targeted to their cellular localisation becaus ...
... cytoplasm, in the lumen of an organelle or in the extracellular environments and those that are membrane attached associated wit a lipid bilayer. They can be integral membrane proteins or they may be peripherally attached to membranes. Proteins are also targeted to their cellular localisation becaus ...
Mutation of exposed hydrophobic amino acids to arginine to
... the protein (Fig. 3). However, differences were rather small. This is in accordance with literature, amino acid substitution usually does not significantly affect the stability [10,19], although important improvements of stability by mutagenesis of a single solvent-exposed residue have been reported ...
... the protein (Fig. 3). However, differences were rather small. This is in accordance with literature, amino acid substitution usually does not significantly affect the stability [10,19], although important improvements of stability by mutagenesis of a single solvent-exposed residue have been reported ...
Tertiary Protein Structure Prediction with Profile Analysis: A Case Study
... The ability to predict protein structure from amino acid sequences alone would be invaluable to scientists everywhere. A pharmaceutical company working on creating a new lifesaving drug, for example, could analyze the sequence of a protein that plays an integral role in the disease with a computer a ...
... The ability to predict protein structure from amino acid sequences alone would be invaluable to scientists everywhere. A pharmaceutical company working on creating a new lifesaving drug, for example, could analyze the sequence of a protein that plays an integral role in the disease with a computer a ...
Genetic backgrounds of each Escherichia coli strain used
... ompT: Mutation in outer-membrane protease that Improves yield of some recombinant proteins. gal: Mutation in galactose metabolism. Blocks galactose utilization. dcm: Cytosine methylation blocked (it makes DNA susceptible to cleavage at CC(A/T)GG sequences by some restriction enzymes). lon: This stra ...
... ompT: Mutation in outer-membrane protease that Improves yield of some recombinant proteins. gal: Mutation in galactose metabolism. Blocks galactose utilization. dcm: Cytosine methylation blocked (it makes DNA susceptible to cleavage at CC(A/T)GG sequences by some restriction enzymes). lon: This stra ...
Bacterial and Firefly Luciferase Genes in Transgenic Plants
... of reduced FMN and long-chain aldehydes with molecular oxygen to yield FMN, H20, corresponding carboxylic acids, and blue-green light (490 nm). The reaction can be considered as a branch of an electron-transport pathway which shunts electrons to oxygen a t the level of flavin. The enzymatic reaction ...
... of reduced FMN and long-chain aldehydes with molecular oxygen to yield FMN, H20, corresponding carboxylic acids, and blue-green light (490 nm). The reaction can be considered as a branch of an electron-transport pathway which shunts electrons to oxygen a t the level of flavin. The enzymatic reaction ...
macromolecules test 1
... Refer to the following diagram, which shows the DNA fingerprint obtained from blood cells at a crime scene. One section of a DNA molecule from the blood cells was multiplied. A restriction enzyme was used to cut the section into six fragments, which were then separated by gel electrophoresis. The sm ...
... Refer to the following diagram, which shows the DNA fingerprint obtained from blood cells at a crime scene. One section of a DNA molecule from the blood cells was multiplied. A restriction enzyme was used to cut the section into six fragments, which were then separated by gel electrophoresis. The sm ...
Infectious Bursal Disease Virus (IBDV)
... The primer and probe mix provided exploits the so-called TaqMan® principle. During PCR amplification, forward and reverse primers hybridize to the IBDV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`-dye and a 3`-quencher. During PCR ...
... The primer and probe mix provided exploits the so-called TaqMan® principle. During PCR amplification, forward and reverse primers hybridize to the IBDV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`-dye and a 3`-quencher. During PCR ...
Article Parallel Histories of Horizontal Gene
... lack additional symbiotic partners (Thao et al. 2000) but still maintain Carsonella strains with highly reduced genomes that have lost genes required for synthesis of some essential amino acids (Nakabachi et al. 2006). Finally, missing Carsonella genes may have been functionally replaced by host-enc ...
... lack additional symbiotic partners (Thao et al. 2000) but still maintain Carsonella strains with highly reduced genomes that have lost genes required for synthesis of some essential amino acids (Nakabachi et al. 2006). Finally, missing Carsonella genes may have been functionally replaced by host-enc ...
... rainbow trout and carp did. The two cDNAs code for two very abundant plasma proteins LALl (105 amino acids) and LAL2 (191 amino acids) that have characteristics of typical mammalian apolipoproteins. However, no definitive assignment of the two lamprey proteins to typical mammalian apolipoprotein cla ...
Characterization of Ubiquitin/Proteasome
... nicotinamide adenine dinucleotide (NAD+) is reduced to NADH by the transfer of hydrogen from glyceraldehyde phosphate by the enzyme glyceraldehyde 3-phosphate dehydrogenase. The overall reaction of glycolysis thus produces two moles of ATP, two moles of pyruvate, and two moles of the reduced NADH fr ...
... nicotinamide adenine dinucleotide (NAD+) is reduced to NADH by the transfer of hydrogen from glyceraldehyde phosphate by the enzyme glyceraldehyde 3-phosphate dehydrogenase. The overall reaction of glycolysis thus produces two moles of ATP, two moles of pyruvate, and two moles of the reduced NADH fr ...
analyzing gene and protein sequences
... -click on any of the accession numbers displayed and you will be able to view information about that sequence such as definition, source, classification, journal articles, comments, amino acid sequence, and the nucleotide sequence. -scroll down to the bottom of the page to see the nucleotide sequenc ...
... -click on any of the accession numbers displayed and you will be able to view information about that sequence such as definition, source, classification, journal articles, comments, amino acid sequence, and the nucleotide sequence. -scroll down to the bottom of the page to see the nucleotide sequenc ...
access full article - Caister Academic Press
... actinomycetale family since they are prolific producers of secondary metabolites. Molecular genetic analyses of these organisms have revealed that the genes encoding their secondary metabolite biosynthetic pathways are clustered and range in size from five to greater than 100 kilobases (Malpartida a ...
... actinomycetale family since they are prolific producers of secondary metabolites. Molecular genetic analyses of these organisms have revealed that the genes encoding their secondary metabolite biosynthetic pathways are clustered and range in size from five to greater than 100 kilobases (Malpartida a ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008.
... Therefore, chitin degradation and chitinases are of biotechnological importance. Chitinases degrade chitin into mainly mono and dimeric units of GlcNac. These enzymes are ubiquitous and are reported from bacteria, yeasts, higher fungi, plants and animals. In plants and animals, their role is mainly ...
... Therefore, chitin degradation and chitinases are of biotechnological importance. Chitinases degrade chitin into mainly mono and dimeric units of GlcNac. These enzymes are ubiquitous and are reported from bacteria, yeasts, higher fungi, plants and animals. In plants and animals, their role is mainly ...
protein factory ingredient info
... faster and more efficiently than any other glutamine peptide on the market. If a company does not tell you the molecular weight of their glutamine peptide product you know you are getting ripped off. Protein Factory's Glutamine Peptides have changed their appearance. It is still from the same manufa ...
... faster and more efficiently than any other glutamine peptide on the market. If a company does not tell you the molecular weight of their glutamine peptide product you know you are getting ripped off. Protein Factory's Glutamine Peptides have changed their appearance. It is still from the same manufa ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.