ProteinPrediction
... that are related by lower but still detectable levels of sequence similarity (and therefore have a common but more ancient evolutionary origin). ...
... that are related by lower but still detectable levels of sequence similarity (and therefore have a common but more ancient evolutionary origin). ...
5 end
... • Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions • These noncoding regions are called intervening sequences, or introns • The other regions are called exons because they are eventually expressed, usually translated into am ...
... • Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions • These noncoding regions are called intervening sequences, or introns • The other regions are called exons because they are eventually expressed, usually translated into am ...
biological background the central dogma of molecular biology
... Initiation: occurs in a specific region on the DNA called the promoter. • The promoter contains sequence elements that bind several transcription factors in combination with RNA polymerase and indicate the first base to be copied into an RNA transcript (the promoter also includes sequences involved ...
... Initiation: occurs in a specific region on the DNA called the promoter. • The promoter contains sequence elements that bind several transcription factors in combination with RNA polymerase and indicate the first base to be copied into an RNA transcript (the promoter also includes sequences involved ...
DNA cloning
... Following the elucidation of the DNA structure and the genetic code, it became clear that many biological secrets were hidden in the sequence of bases in DNA. Technical and biological discoveries in the 1970s led to a new era of DNA analysis and manipulation. Key among these was the discovery of two ...
... Following the elucidation of the DNA structure and the genetic code, it became clear that many biological secrets were hidden in the sequence of bases in DNA. Technical and biological discoveries in the 1970s led to a new era of DNA analysis and manipulation. Key among these was the discovery of two ...
Sequence and transcription analysis of the Petunia mitochondrial
... labelled at the BamHI site immediately outside the coding region will not be protected from S1 nuclease digestion by transcripts from any other homologous gene. ...
... labelled at the BamHI site immediately outside the coding region will not be protected from S1 nuclease digestion by transcripts from any other homologous gene. ...
Mechanism of peptide bond formation on ribosomes
... Mechanism of peptide bond formation on ribosomes D. P. Burma In spite of extensive studies carried out on structure and function of ribosomes during the last four decades or so, the crucial information on the mechanism of peptide bond formation was missing. However, with the very recent elucidation ...
... Mechanism of peptide bond formation on ribosomes D. P. Burma In spite of extensive studies carried out on structure and function of ribosomes during the last four decades or so, the crucial information on the mechanism of peptide bond formation was missing. However, with the very recent elucidation ...
Searching for Genes
... All branches on the tree of life share common roots. One way to study those roots is to look to DNA sequences. So, when the Roberts lab began studying the evolution of cellulose synthesis, one place they looked was at the available DNA sequence information. Even now, as they expand their studies to ...
... All branches on the tree of life share common roots. One way to study those roots is to look to DNA sequences. So, when the Roberts lab began studying the evolution of cellulose synthesis, one place they looked was at the available DNA sequence information. Even now, as they expand their studies to ...
Part 2 - people.iup.edu
... Concept 5.4: Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells • Proteins have more chemical and physical versatility than any other type of macromolecule • Protein functions include structural support, storage, t ...
... Concept 5.4: Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells • Proteins have more chemical and physical versatility than any other type of macromolecule • Protein functions include structural support, storage, t ...
Protein Folding Problem
... The initial stages of folding must be nearly random, but if the entire process was a random search it would require too much time. Consider a 100 residue protein. If each residue is considered to have just 3 possible conformations the total number of conformations of the protein is 3100. Conformatio ...
... The initial stages of folding must be nearly random, but if the entire process was a random search it would require too much time. Consider a 100 residue protein. If each residue is considered to have just 3 possible conformations the total number of conformations of the protein is 3100. Conformatio ...
Characterization of Tbc2, a nucleus
... W. Zerges’s present address is Biology Department, Concordia University, 1445 Maisonneuve W., Montreal, Quebec, H3G 1M8, Canada. Key words: chloroplast; translation; Chlamydomonas; photosynthetic mutant; protein complex ...
... W. Zerges’s present address is Biology Department, Concordia University, 1445 Maisonneuve W., Montreal, Quebec, H3G 1M8, Canada. Key words: chloroplast; translation; Chlamydomonas; photosynthetic mutant; protein complex ...
Gen660_Lecture12B_NetworkEvo_2014
... How do regulatory networks evolve? Module = group of genes co-regulated by the same regulatory system * Evolution of individual gene targets Gain or loss of genes from a module * Evolution of activating signals Change in responsiveness but not regulators * Wholesale evolution of the entire module Tr ...
... How do regulatory networks evolve? Module = group of genes co-regulated by the same regulatory system * Evolution of individual gene targets Gain or loss of genes from a module * Evolution of activating signals Change in responsiveness but not regulators * Wholesale evolution of the entire module Tr ...
NON-CANONICAL TRANSCRIPTION INITIATION: THE EXPANDING
... In the cell, the nicotinamide ribonucleoside moiety can be removed by a Nudix enzyme. Nudix enzymes are pyrophosphohydrolases that catalyze the breakage of pyrophosphate bonds in a range of substrates (McLennan, 2013). Consistently, deletion of nudC resulted in about a twofold increase in the abunda ...
... In the cell, the nicotinamide ribonucleoside moiety can be removed by a Nudix enzyme. Nudix enzymes are pyrophosphohydrolases that catalyze the breakage of pyrophosphate bonds in a range of substrates (McLennan, 2013). Consistently, deletion of nudC resulted in about a twofold increase in the abunda ...
GO: The Gene Ontology
... GO:L-xylulose reductase activity ; GO:0050038 GO:4-oxoproline reductase activity ; GO:0016617 GO:retinol dehydrogenase activity ; GO:0004745 ...
... GO:L-xylulose reductase activity ; GO:0050038 GO:4-oxoproline reductase activity ; GO:0016617 GO:retinol dehydrogenase activity ; GO:0004745 ...
Align sequence to structure - Computational Bioscience Program
... • Proteins that have similar sequences (i.e., related by evolution) are likely to have similar three-dimensional structures 1. BLAST sequence of Interest against PDB to identify a template •Multiple templates can be used if desired •Templates with Ligands bound can be used to identify binding sites ...
... • Proteins that have similar sequences (i.e., related by evolution) are likely to have similar three-dimensional structures 1. BLAST sequence of Interest against PDB to identify a template •Multiple templates can be used if desired •Templates with Ligands bound can be used to identify binding sites ...
Lecture_10
... cellular proteins. GFP fluoresces green when exposed to blue light, allowing the determination of the cellular location of the attached protein. ...
... cellular proteins. GFP fluoresces green when exposed to blue light, allowing the determination of the cellular location of the attached protein. ...
Molecular Biology Fourth Edition
... Certain DNA Sequences Adopt Unusual Structures • Other sequence-specific DNA structures have been detected, within larger chromosomes, that may affect the function and metabolism of the DNA segments in their immediate vicinity. • For example, certain repetitive sequences can bend the DNA helix in a ...
... Certain DNA Sequences Adopt Unusual Structures • Other sequence-specific DNA structures have been detected, within larger chromosomes, that may affect the function and metabolism of the DNA segments in their immediate vicinity. • For example, certain repetitive sequences can bend the DNA helix in a ...
Amino Acids Proteins, and Enzymes
... (From ExPASy Biochemical Pathways; http://www.expasy.org/cgi-bin/show_thumbnails.pl?2) ...
... (From ExPASy Biochemical Pathways; http://www.expasy.org/cgi-bin/show_thumbnails.pl?2) ...
Lecture 11 - Class I and Class III Factors
... Model of preinitiation complex formation on TATA-less promoters recognized by all three types of polymerases 1) Assembly factor binds first (UBP, Sp1 and TFIIIC in class I, II and III promoters, respectively) 2) This attracts another factor that contains TBP (these are SL1, TFIID or TFIIIB in class ...
... Model of preinitiation complex formation on TATA-less promoters recognized by all three types of polymerases 1) Assembly factor binds first (UBP, Sp1 and TFIIIC in class I, II and III promoters, respectively) 2) This attracts another factor that contains TBP (these are SL1, TFIID or TFIIIB in class ...
6 Characterization of Casein and Bovine Serum Albumin (BSA)
... cellular components using basic chemical techniques. Once a protein has been isolated, one can initiate characterization studies, information such as pH- and heat-stability, that could prove helpful in ascertaining the protein’s structure and/or function without knowing its amino acid sequence. Many ...
... cellular components using basic chemical techniques. Once a protein has been isolated, one can initiate characterization studies, information such as pH- and heat-stability, that could prove helpful in ascertaining the protein’s structure and/or function without knowing its amino acid sequence. Many ...
BMMB597E_lecture3
... • In CATH, proteins with very similar structures, sequences and functions are grouped into sequence families. • A homologous superfamily contains proteins for which similarity of sequence and structure gives evidence of common ancestry • A topology or fold family comprises sets of homologous superfa ...
... • In CATH, proteins with very similar structures, sequences and functions are grouped into sequence families. • A homologous superfamily contains proteins for which similarity of sequence and structure gives evidence of common ancestry • A topology or fold family comprises sets of homologous superfa ...
Control of Gene Expression
... by (1) controlling when and how often a given gene is transcribed, (2) controlling how an RNA transcript is spliced or otherwise processed, (3) selecting which mRNAs are exported from the nucleus to the cytosol, (4) selectively degrading certain mRNA molecules, (5) selecting which mRNAs are translat ...
... by (1) controlling when and how often a given gene is transcribed, (2) controlling how an RNA transcript is spliced or otherwise processed, (3) selecting which mRNAs are exported from the nucleus to the cytosol, (4) selectively degrading certain mRNA molecules, (5) selecting which mRNAs are translat ...
Epigenetics of Cancer
... • Any process that alters gene activity without changing the DNA sequence and leads to modifications that can be transmitted to daughter cells. • Epigenomics: global study of epigenetic changes across the entire genome ...
... • Any process that alters gene activity without changing the DNA sequence and leads to modifications that can be transmitted to daughter cells. • Epigenomics: global study of epigenetic changes across the entire genome ...
Detailed characterization of the interactions between hepatitis C virus and host proteins.
... between HCV and host will determine the outcome of the viral infection. To characterize these processes in details, several novel viral-host interactions have been identified through yeast-twohybrid screen, proteomic and bioinformatic approaches. For the first part of the project, we aim to determin ...
... between HCV and host will determine the outcome of the viral infection. To characterize these processes in details, several novel viral-host interactions have been identified through yeast-twohybrid screen, proteomic and bioinformatic approaches. For the first part of the project, we aim to determin ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.