Review Non-ribosomal peptide synthetases: Identifying the cryptic
... biosynthetic NRPS-PKS hybrid gene cluster discovered in M. tuberculosis (Quadri et al. 1998). Presence of such a small protein encoding gene was puzzling in NRPS-PKS cluster, and hence its study remained dormant for quite a long time. Later, consistent identification of MbtH homologs in other second ...
... biosynthetic NRPS-PKS hybrid gene cluster discovered in M. tuberculosis (Quadri et al. 1998). Presence of such a small protein encoding gene was puzzling in NRPS-PKS cluster, and hence its study remained dormant for quite a long time. Later, consistent identification of MbtH homologs in other second ...
Correlation of an Immobilized Digestive Enzyme Assay with Poultry
... tide bonds in the sample using acid hydrolysis (24 h), whereas the optimized IDEA SBM kit uses nitrogen combustion analysis (15 min) to calculate a percent protein. Calculation of the IDEA value for both assays uses a parameter in the denominator to provide sample protein comparability. The IDEA SBM ...
... tide bonds in the sample using acid hydrolysis (24 h), whereas the optimized IDEA SBM kit uses nitrogen combustion analysis (15 min) to calculate a percent protein. Calculation of the IDEA value for both assays uses a parameter in the denominator to provide sample protein comparability. The IDEA SBM ...
BIOCHEMISTRY
... treatment. Biochemical tests can also be used to detect complications of treatment and are extensively used to screen for possible drug toxicity, particularly in trials, but also in some cases when a drug is in established use. Biochemical tests are widely used to determine whether a condition is pr ...
... treatment. Biochemical tests can also be used to detect complications of treatment and are extensively used to screen for possible drug toxicity, particularly in trials, but also in some cases when a drug is in established use. Biochemical tests are widely used to determine whether a condition is pr ...
Kinetics and Mechanism of Uncatalyzed and Ag (I) Catalyzed
... N-bromo-benzenesulphonamide [11], in both acid and alkaline media have been studied. Although, various types of the reaction models have been suggested by different researchers [12-16], the specific details are yet to be discovered. Also, there are still controversies regarding the mechanistic pathw ...
... N-bromo-benzenesulphonamide [11], in both acid and alkaline media have been studied. Although, various types of the reaction models have been suggested by different researchers [12-16], the specific details are yet to be discovered. Also, there are still controversies regarding the mechanistic pathw ...
Glucose Polyester Biosynthesis. Purification and
... on a SDS-PAGE column. Intensities of the 34- and 24-kD bands were similar in these two samples (not shown), suggesting that they are likely candidates for the enzyme. To confirm the identity of the proteins responsible for activity, native PAGE was performed using a ConApurified enzyme preparation. ...
... on a SDS-PAGE column. Intensities of the 34- and 24-kD bands were similar in these two samples (not shown), suggesting that they are likely candidates for the enzyme. To confirm the identity of the proteins responsible for activity, native PAGE was performed using a ConApurified enzyme preparation. ...
The integration of flow reactors into synthetic organic chemistry
... up to six additional manipulations (work-up and purification) can be required. Interestingly, these supplementary operations, which are essential but costly (in time and resources), add very little intrinsic value to the compounds; they are necessary only because of inefficiencies in our current syn ...
... up to six additional manipulations (work-up and purification) can be required. Interestingly, these supplementary operations, which are essential but costly (in time and resources), add very little intrinsic value to the compounds; they are necessary only because of inefficiencies in our current syn ...
Chapter 18 Metabolic Pathways and Energy Production
... A. coenzyme used in oxidation of carbon-oxygen bonds NAD+ B. reduced form of flavin adenine dinucleotide FADH2 C. used to transfer acetyl groups Coenzyme A D. oxidized form of flavin adenine dinucleotide FAD E. the coenzyme after C=O bond formation NADH + H+ ...
... A. coenzyme used in oxidation of carbon-oxygen bonds NAD+ B. reduced form of flavin adenine dinucleotide FADH2 C. used to transfer acetyl groups Coenzyme A D. oxidized form of flavin adenine dinucleotide FAD E. the coenzyme after C=O bond formation NADH + H+ ...
Study on the degradability of poly(ester amide)s derived from the... acids glycine, and l-alanine containing a variable amide/ester ratio
... calibration. Thermogravimetric analysis was carried out with a Perkin±Elmer TGA-6 thermobalance. Plates of 1:5 cm £ 1:5 cm £ 200 mm were cut off from ®lms prepared by melt pressing 200 mg of powder samples. Films of the PDAxAHAy8 series were obtained by applying 1 metric ton for 1 min at the fusion ...
... calibration. Thermogravimetric analysis was carried out with a Perkin±Elmer TGA-6 thermobalance. Plates of 1:5 cm £ 1:5 cm £ 200 mm were cut off from ®lms prepared by melt pressing 200 mg of powder samples. Films of the PDAxAHAy8 series were obtained by applying 1 metric ton for 1 min at the fusion ...
Classification of pseudo pairs between nucleotide bases and amino
... single and bifurcated hydrogen bonds observed between bases and amino acids are classified and annotated. Only 5 of the 20 usual amino acid residues, Asn, Gln, Asp, Glu and Arg, are able to orient in a coplanar fashion in order to form pseudo pairs with nucleotide bases through two hydrogen bonds. T ...
... single and bifurcated hydrogen bonds observed between bases and amino acids are classified and annotated. Only 5 of the 20 usual amino acid residues, Asn, Gln, Asp, Glu and Arg, are able to orient in a coplanar fashion in order to form pseudo pairs with nucleotide bases through two hydrogen bonds. T ...
vapor phase transport vpc materials synthesis, crystal growth
... The reactor consists of three inner quartz tubes, which supply the reactive gases, InCl3, GaCl3 (N2 carrier) and NH3, and an outer quartz tube, which supplies inert gas (N2) and houses the reaction in a horizontal tube furnace. Two independently controlled heating tapes were used to tune the vapour ...
... The reactor consists of three inner quartz tubes, which supply the reactive gases, InCl3, GaCl3 (N2 carrier) and NH3, and an outer quartz tube, which supplies inert gas (N2) and houses the reaction in a horizontal tube furnace. Two independently controlled heating tapes were used to tune the vapour ...
to Sample Chapter
... 14. Give reason: At the time of cooling in laboratory method used for glucose preparation, alcohol is added. Ans. i. Hydrolysis of cane sugar gives a mixture of glucose and fructose. ii. Glucose is insoluble in alcohol and crystallized out first while Fructose being soluble in alcohol it remains in ...
... 14. Give reason: At the time of cooling in laboratory method used for glucose preparation, alcohol is added. Ans. i. Hydrolysis of cane sugar gives a mixture of glucose and fructose. ii. Glucose is insoluble in alcohol and crystallized out first while Fructose being soluble in alcohol it remains in ...
UNIT-II - E
... 10. In either case, the Ac is not to be confused with the abbreviation for the chemical element actinium. Acetic acid has the molecular formula C2H4O2 (empirical formula CH2O). 11. To emphasize the role of the active hydrogen in forming the salt sodium acetate, some people write the molecular formul ...
... 10. In either case, the Ac is not to be confused with the abbreviation for the chemical element actinium. Acetic acid has the molecular formula C2H4O2 (empirical formula CH2O). 11. To emphasize the role of the active hydrogen in forming the salt sodium acetate, some people write the molecular formul ...
Fatty acid and phospholipid metabolism in prokaryotes
... auxotrophy on a strain, such as a requirement for unsaturated fatty acids or glycerol phosphate. Such mutants have generally lost the ability to produce a key biosynthetic enzyme (e.g.fabA mutants, which require supplementation with unsaturated fatty acids), or they may be more complex (e.g. plsB mu ...
... auxotrophy on a strain, such as a requirement for unsaturated fatty acids or glycerol phosphate. Such mutants have generally lost the ability to produce a key biosynthetic enzyme (e.g.fabA mutants, which require supplementation with unsaturated fatty acids), or they may be more complex (e.g. plsB mu ...
A Simple Method for Displaying the Hydropathic Character of a Protein
... Ideally, the most satisfying way to determine the hydrophobic or hydrophilic inclinations of a given amino acid side-chain (i.e. its hydropathyt) would be to measure its partition coefficient between water and a non-interacting, isotropic phase and to calculate from that partition coefficient a tran ...
... Ideally, the most satisfying way to determine the hydrophobic or hydrophilic inclinations of a given amino acid side-chain (i.e. its hydropathyt) would be to measure its partition coefficient between water and a non-interacting, isotropic phase and to calculate from that partition coefficient a tran ...
Ube2W conjugates ubiquitin to α-amino groups of protein N
... The covalent attachment of the protein ubiquitin to intracellular proteins by a process known as ubiquitylation regulates almost all major cellular systems, predominantly by regulating protein turnover. Ubiquitylation requires the co-ordinated action of three enzymes termed E1, E2 and E3, and typica ...
... The covalent attachment of the protein ubiquitin to intracellular proteins by a process known as ubiquitylation regulates almost all major cellular systems, predominantly by regulating protein turnover. Ubiquitylation requires the co-ordinated action of three enzymes termed E1, E2 and E3, and typica ...
The Role of the Krebs Cycle in Conjugation in
... The results obtained by supplementing washed minimal agar with aspartic acid suggested that this substance was necessary for zygote formation. Zygotes which had been preformed in nutrient broth yielded recombinants with the same efficiency on medium WMAB, as on medium MAB,. The role of aspartic acid ...
... The results obtained by supplementing washed minimal agar with aspartic acid suggested that this substance was necessary for zygote formation. Zygotes which had been preformed in nutrient broth yielded recombinants with the same efficiency on medium WMAB, as on medium MAB,. The role of aspartic acid ...
Carbon conversion efficiency and central - Shachar
... and from the labeling data. We used existing models of metabolic pathways in non-photosynthetic tissues to which oil and protein synthesis pathways were added (Schwender et al., 2006). Incorporation of [U-14C4]malate and [U-14C5]glutamine into starch and cell wall (Figure 2b,c) indicated a gluconeog ...
... and from the labeling data. We used existing models of metabolic pathways in non-photosynthetic tissues to which oil and protein synthesis pathways were added (Schwender et al., 2006). Incorporation of [U-14C4]malate and [U-14C5]glutamine into starch and cell wall (Figure 2b,c) indicated a gluconeog ...
The Role of the Krebs Cycle in Conjugation in
... The results obtained by supplementing washed minimal agar with aspartic acid suggested that this substance was necessary for zygote formation. Zygotes which had been preformed in nutrient broth yielded recombinants with the same efficiency on medium WMAB, as on medium MAB,. The role of aspartic acid ...
... The results obtained by supplementing washed minimal agar with aspartic acid suggested that this substance was necessary for zygote formation. Zygotes which had been preformed in nutrient broth yielded recombinants with the same efficiency on medium WMAB, as on medium MAB,. The role of aspartic acid ...
Tendency for Local Repetitiveness in Amino Acid Usages in Modern
... amino acids R and K in correlation with the G C content of the corresponding genome DNA, raised the possibility that local unevenness of genome DNA structure may be related to the tendency for repetitiveness of the genome (Nishizawa & Nishizawa, 1998). It has also been reported that eukaryotes, bu ...
... amino acids R and K in correlation with the G C content of the corresponding genome DNA, raised the possibility that local unevenness of genome DNA structure may be related to the tendency for repetitiveness of the genome (Nishizawa & Nishizawa, 1998). It has also been reported that eukaryotes, bu ...
The Occurrence and Location of Teichoic Acids in
... finding, and the absence of intracellular glycerol teichoic acid in those walls which contained the ribitol compound, indicated that little or no cross-contaminationof wall and cell material occurred during the isolation procedure. Faint traces of ribitol derivatives were detected in the cell conten ...
... finding, and the absence of intracellular glycerol teichoic acid in those walls which contained the ribitol compound, indicated that little or no cross-contaminationof wall and cell material occurred during the isolation procedure. Faint traces of ribitol derivatives were detected in the cell conten ...
Phosphate stabilizing compositions
... Component (b) is an anionic carboxylic polymer or a salt thereof that stabilizes phosphates against precipitation in an aqueous system. For purposes of describing this invention, polymer ...
... Component (b) is an anionic carboxylic polymer or a salt thereof that stabilizes phosphates against precipitation in an aqueous system. For purposes of describing this invention, polymer ...