IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
... spores/ml), 1ml of C. globosum spores (2.34 x 106 spores/ml) and 1ml of S. cerevisiae cells (3.96 x 106 cells/ml). The contents of the other three flasks were not inoculated and these served as controls. The inoculated samples were mixed thoroughly with the aid of surface-sterilized spatula. This he ...
... spores/ml), 1ml of C. globosum spores (2.34 x 106 spores/ml) and 1ml of S. cerevisiae cells (3.96 x 106 cells/ml). The contents of the other three flasks were not inoculated and these served as controls. The inoculated samples were mixed thoroughly with the aid of surface-sterilized spatula. This he ...
Alterations in oxidative phosphorylation complex proteins in the
... overexpression of MKK6 protected cultured cardiac myocytes against various types of stresses in a p38-dependent manner (54, 55). This protective effect has since been demonstrated by other laboratories, as well (28, 47). In genetically modified mice harboring an ␣-myosin heavy chain-driven MKK6 tran ...
... overexpression of MKK6 protected cultured cardiac myocytes against various types of stresses in a p38-dependent manner (54, 55). This protective effect has since been demonstrated by other laboratories, as well (28, 47). In genetically modified mice harboring an ␣-myosin heavy chain-driven MKK6 tran ...
Characterization of Lipid Rafts from Medicago
... that their detergent-insolubility property correlates with functional properties (for review, see He et al., 2005). The emerging use of microscopic analyses to localize proteins in lipids raft and to deduce their lateral mobility should give new insights into in vivo lipid raft protein composition, ...
... that their detergent-insolubility property correlates with functional properties (for review, see He et al., 2005). The emerging use of microscopic analyses to localize proteins in lipids raft and to deduce their lateral mobility should give new insights into in vivo lipid raft protein composition, ...
Ars Pharmaceutica - Facultad de Farmacia
... Aim: The objective of this study was to evaluate the integration of the AccQ•Tag derivatization system with the Finnigan Surveyor Plus HPLC system to determine the amino acids (aa) composition of earthworm meal protein post-hydrolysis. Materials and Methods: In lab cultivated earthworms (Eisenia and ...
... Aim: The objective of this study was to evaluate the integration of the AccQ•Tag derivatization system with the Finnigan Surveyor Plus HPLC system to determine the amino acids (aa) composition of earthworm meal protein post-hydrolysis. Materials and Methods: In lab cultivated earthworms (Eisenia and ...
Fast and simple purification of GST fusion proteins using prepacked
... preparative separations. The columns can be used separately or connected together in series. Columns are made of medical-grade polypropylene, a biocompatible material that does not interact with biomolecules. Top and bottom frits are manufactured from porous polyethylene. The frits allow high flow r ...
... preparative separations. The columns can be used separately or connected together in series. Columns are made of medical-grade polypropylene, a biocompatible material that does not interact with biomolecules. Top and bottom frits are manufactured from porous polyethylene. The frits allow high flow r ...
The Impact of Non-Enzymatic Reactions and Enzyme Promiscuity on
... methionine residues absorb oxidizing agents by positioning on the exposed protein surface and surrounding the active site, thereby preventing more severe oxidative damage on targeted proteins [33,34]. This oxidation of methionine residues at the protein surface can proceed without significant reduct ...
... methionine residues absorb oxidizing agents by positioning on the exposed protein surface and surrounding the active site, thereby preventing more severe oxidative damage on targeted proteins [33,34]. This oxidation of methionine residues at the protein surface can proceed without significant reduct ...
pGEX-5X-3 GST Expression Vector
... CaCl2; incubation temperature, 25°C; incubation time, 2-16 hours (1, 2, 4). The molecular weight of factor Xa (bovine) is approximately 48 kDa and the protease is available as a stand alone product. Multiple Cloning region and protease cleavage site ...
... CaCl2; incubation temperature, 25°C; incubation time, 2-16 hours (1, 2, 4). The molecular weight of factor Xa (bovine) is approximately 48 kDa and the protease is available as a stand alone product. Multiple Cloning region and protease cleavage site ...
Expression and V (D) J recombination activity of mutated RAG
... immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination fun ...
... immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination fun ...
Enzymes - bYTEBoss
... works for fats. Fats are large molecules, made up of the smaller particles called fatty acids and glycerol ...
... works for fats. Fats are large molecules, made up of the smaller particles called fatty acids and glycerol ...
Gonococcal outer-membrane protein PIB
... epitope recognized by the antibody. To identify precisely the epitope recognized by mAb SM24, a series of hexapeptides were synthesized between residues 193 and 204, together with a series of smaller peptides centred on residue 198. Antibody SM24 reacted with five hexapeptides, all of which containe ...
... epitope recognized by the antibody. To identify precisely the epitope recognized by mAb SM24, a series of hexapeptides were synthesized between residues 193 and 204, together with a series of smaller peptides centred on residue 198. Antibody SM24 reacted with five hexapeptides, all of which containe ...
Enzymes - africangreyparrott.com
... They require a specific temperature And if it’s not exact they can be denatured ...
... They require a specific temperature And if it’s not exact they can be denatured ...
Involvement of the glycine-rich RNA
... The plant glycine-rich RNA-binding proteins (GRP) are a major class of proteins known to be implicated in plant response to abiotic and biotic stress. Recent studies regarding this type of proteins focused mostly on two of them, namely GRP2 and GRP7, which are mainly involved in osmotic and oxidativ ...
... The plant glycine-rich RNA-binding proteins (GRP) are a major class of proteins known to be implicated in plant response to abiotic and biotic stress. Recent studies regarding this type of proteins focused mostly on two of them, namely GRP2 and GRP7, which are mainly involved in osmotic and oxidativ ...
Essential amino acid
... 27.2 Amino Acid Metabolism: An Overview • The carbon atoms of amino acids are converted to compounds that can enter the citric acid cycle. • They continue through the citric acid cycle to give CO2 and energy stored in ATP. • About 10–20% of our energy is produced in ...
... 27.2 Amino Acid Metabolism: An Overview • The carbon atoms of amino acids are converted to compounds that can enter the citric acid cycle. • They continue through the citric acid cycle to give CO2 and energy stored in ATP. • About 10–20% of our energy is produced in ...
FREE Sample Here - We can offer most test bank and
... Ans: Living things are composed primarily of macromolecules, polymers of simple compounds of just a few different types. The properties of these polymers are determined by their sequence of monomers and these can be combined in many different ways. Diversity is thus achieved through the nearly limit ...
... Ans: Living things are composed primarily of macromolecules, polymers of simple compounds of just a few different types. The properties of these polymers are determined by their sequence of monomers and these can be combined in many different ways. Diversity is thus achieved through the nearly limit ...
FREE Sample Here
... Ans: Living things are composed primarily of macromolecules, polymers of simple compounds of just a few different types. The properties of these polymers are determined by their sequence of monomers and these can be combined in many different ways. Diversity is thus achieved through the nearly limit ...
... Ans: Living things are composed primarily of macromolecules, polymers of simple compounds of just a few different types. The properties of these polymers are determined by their sequence of monomers and these can be combined in many different ways. Diversity is thus achieved through the nearly limit ...
Effect of LIVNITRO on Protein Anabolism
... are characterized by suppressed protein breakdown and via that mechanism lead to a greater anabolic response. This explains why when net protein synthesis is measured, the relationship between amino acid availability and net gain remains linear, without any apparent plateau of effect at higher level ...
... are characterized by suppressed protein breakdown and via that mechanism lead to a greater anabolic response. This explains why when net protein synthesis is measured, the relationship between amino acid availability and net gain remains linear, without any apparent plateau of effect at higher level ...
Universal strategies in research and drug discovery based on protein
... be obscured by extensive cross-talk between pathways. For instance, the inhibition of an enzyme in a biochemical pathway may trigger a whole series of parallel changes in several pathways, ultimately resulting in the initiation of a complex remodelling of gene expression. To pinpoint the small-molec ...
... be obscured by extensive cross-talk between pathways. For instance, the inhibition of an enzyme in a biochemical pathway may trigger a whole series of parallel changes in several pathways, ultimately resulting in the initiation of a complex remodelling of gene expression. To pinpoint the small-molec ...
Proteomic Approach to Identify Novel
... 25% in primary metabolism such as pyruvate decarboxylation, citric acid cycle, and amino acid and nucleotide metabolism. Five identified proteins represent chaperones; others are involved in molecular transport or protection against O2. Only two identified proteins form part of the mitochondrial pro ...
... 25% in primary metabolism such as pyruvate decarboxylation, citric acid cycle, and amino acid and nucleotide metabolism. Five identified proteins represent chaperones; others are involved in molecular transport or protection against O2. Only two identified proteins form part of the mitochondrial pro ...
the elastin gene
... significance of "acidic" and "basic" keratins in this respect? 6. What are the main crosslinks in keratin? How can they be broken to partially solubilise the protein? How does increased crosslinking affect keratin's properties? 7. Keratin is the main component of hair. How are individual keratin mol ...
... significance of "acidic" and "basic" keratins in this respect? 6. What are the main crosslinks in keratin? How can they be broken to partially solubilise the protein? How does increased crosslinking affect keratin's properties? 7. Keratin is the main component of hair. How are individual keratin mol ...
Indian Agricultural Research Institute, New Delhi
... Approaches for Genetic Engineering Increasing proteins rich in essential amino acids in coarse cereals: • Genes encoding lysine rich proteins • beta-amylase (5%), protein Z (7.1%), chymotrypsin inhibitors CI-I (9.5%) and CI-2 (11.5%) and hordeothionin of barley Reducing the synthesis of proteins poo ...
... Approaches for Genetic Engineering Increasing proteins rich in essential amino acids in coarse cereals: • Genes encoding lysine rich proteins • beta-amylase (5%), protein Z (7.1%), chymotrypsin inhibitors CI-I (9.5%) and CI-2 (11.5%) and hordeothionin of barley Reducing the synthesis of proteins poo ...
Heterotrimeric G Protein–Coupled Signaling in Plants
... of all helices. Until the plant Gα-subunit structure and function were studied, the only function ascribed to the helical domain was an interaction with the GoLoco motif from RGS14 (46). The Ras domain and the domain linkers contain the residues that contact GPCRs (in animals), RGS proteins, and the ...
... of all helices. Until the plant Gα-subunit structure and function were studied, the only function ascribed to the helical domain was an interaction with the GoLoco motif from RGS14 (46). The Ras domain and the domain linkers contain the residues that contact GPCRs (in animals), RGS proteins, and the ...
Identification of a GDP-L-fucose: polypeptide fucosyltransferase and
... Experiments using factor VII EGF-1 as acceptor substrate showed O-fucosyltransferase activity present in rat and hamster liver and CHO cell homogenates. CHO cell paste was used as the source of enzyme in all subsequent experiments unless otherwise noted, because it had relatively higher activity tha ...
... Experiments using factor VII EGF-1 as acceptor substrate showed O-fucosyltransferase activity present in rat and hamster liver and CHO cell homogenates. CHO cell paste was used as the source of enzyme in all subsequent experiments unless otherwise noted, because it had relatively higher activity tha ...
Protein
Proteins (/ˈproʊˌtiːnz/ or /ˈproʊti.ɨnz/) are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues. Proteins perform a vast array of functions within living organisms, including catalyzing metabolic reactions, DNA replication, responding to stimuli, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific three-dimensional structure that determines its activity.A linear chain of amino acid residues is called a polypeptide. A protein contains at least one long polypeptide. Short polypeptides, containing less than about 20-30 residues, are rarely considered to be proteins and are commonly called peptides, or sometimes oligopeptides. The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. The sequence of amino acid residues in a protein is defined by the sequence of a gene, which is encoded in the genetic code. In general, the genetic code specifies 20 standard amino acids; however, in certain organisms the genetic code can include selenocysteine and—in certain archaea—pyrrolysine. Shortly after or even during synthesis, the residues in a protein are often chemically modified by posttranslational modification, which alters the physical and chemical properties, folding, stability, activity, and ultimately, the function of the proteins. Sometimes proteins have non-peptide groups attached, which can be called prosthetic groups or cofactors. Proteins can also work together to achieve a particular function, and they often associate to form stable protein complexes.Once formed, proteins only exist for a certain period of time and are then degraded and recycled by the cell's machinery through the process of protein turnover. A protein's lifespan is measured in terms of its half-life and covers a wide range. They can exist for minutes or years with an average lifespan of 1–2 days in mammalian cells. Abnormal and or misfolded proteins are degraded more rapidly either due to being targeted for destruction or due to being unstable.Like other biological macromolecules such as polysaccharides and nucleic acids, proteins are essential parts of organisms and participate in virtually every process within cells. Many proteins are enzymes that catalyze biochemical reactions and are vital to metabolism. Proteins also have structural or mechanical functions, such as actin and myosin in muscle and the proteins in the cytoskeleton, which form a system of scaffolding that maintains cell shape. Other proteins are important in cell signaling, immune responses, cell adhesion, and the cell cycle. Proteins are also necessary in animals' diets, since animals cannot synthesize all the amino acids they need and must obtain essential amino acids from food. Through the process of digestion, animals break down ingested protein into free amino acids that are then used in metabolism.Proteins may be purified from other cellular components using a variety of techniques such as ultracentrifugation, precipitation, electrophoresis, and chromatography; the advent of genetic engineering has made possible a number of methods to facilitate purification. Methods commonly used to study protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray crystallography, nuclear magnetic resonance and mass spectrometry.