Bioinformatik - Chair of Computational Biology
... polymerase-based technique. This technique is based on the ability of a specific nucleotide (dideoxy nucleotide) to terminate the DNA polymerase reaction. These nucleotides do not have a free 3'-OH group, an absolute requirement for DNA polymerase activity. Thus, any time this nucleotide is inserted ...
... polymerase-based technique. This technique is based on the ability of a specific nucleotide (dideoxy nucleotide) to terminate the DNA polymerase reaction. These nucleotides do not have a free 3'-OH group, an absolute requirement for DNA polymerase activity. Thus, any time this nucleotide is inserted ...
Using the Hepatitis C Virus RNA-Dependent RNA Polymerase as a
... size and secondary structure depending on the specific requirements for replication in a given virus (i.e., replicating single- or double-stranded RNA/DNA genomes). The fingers and thumb domains of different polymerases have similar positions with respect to the palm, which contains the active site ...
... size and secondary structure depending on the specific requirements for replication in a given virus (i.e., replicating single- or double-stranded RNA/DNA genomes). The fingers and thumb domains of different polymerases have similar positions with respect to the palm, which contains the active site ...
Degenerate PCR - Yale School of Medicine
... 2) the use of inosine as a "neutral" base Inosine is a purine (which occurs naturally in tRNAs) that can form base pairs with cytidine, thymidine, and adenosine (although the inosine:adenosine pairing presumably doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalt ...
... 2) the use of inosine as a "neutral" base Inosine is a purine (which occurs naturally in tRNAs) that can form base pairs with cytidine, thymidine, and adenosine (although the inosine:adenosine pairing presumably doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalt ...
Protocol
... requires short strands RNA that are called primers- the two different primers only bind at ends of a segment of DNA within the gene of interest- the PLC-gamma geneso that you make many copies of the bit of DNA located within the primer binding sites), and (4) the proper buffer components for the pol ...
... requires short strands RNA that are called primers- the two different primers only bind at ends of a segment of DNA within the gene of interest- the PLC-gamma geneso that you make many copies of the bit of DNA located within the primer binding sites), and (4) the proper buffer components for the pol ...
Nucleic Acids - hrsbstaff.ednet.ns.ca
... The base is attached to the C1 The phosphate is linked to the C5 The phosphate of another nucleotide will link to the C3 ...
... The base is attached to the C1 The phosphate is linked to the C5 The phosphate of another nucleotide will link to the C3 ...
Genetics Project
... Collects and passes out group materials and work Communicates with the teacher Assigns a ‘Daily Participation Grade’ for each member of the group Collects and grades homework for the group when necessary ...
... Collects and passes out group materials and work Communicates with the teacher Assigns a ‘Daily Participation Grade’ for each member of the group Collects and grades homework for the group when necessary ...
1. If the inside ends
... 1. Replicative transposase cuts only one strand at the junction. 2. Nonreplicative transposase makes cuts in both strands in the junction. • The similarity between replicative and nonreplicative transposition 1. The cut 5’ ends of the target DNA are joined to the free 3’ ends of the transposon. 2. T ...
... 1. Replicative transposase cuts only one strand at the junction. 2. Nonreplicative transposase makes cuts in both strands in the junction. • The similarity between replicative and nonreplicative transposition 1. The cut 5’ ends of the target DNA are joined to the free 3’ ends of the transposon. 2. T ...
Print Article - International Journal of Current Research and
... denaturation and hydrolysis at high temperatures i.e., there is a greater burden of both chemical degradation and duplex destabilization (Robb and Maeder, 1998; Robb and Clark, 1999). At these temperatures, not only the DNA double helix unwind, but it also induces various chemical modifications that ...
... denaturation and hydrolysis at high temperatures i.e., there is a greater burden of both chemical degradation and duplex destabilization (Robb and Maeder, 1998; Robb and Clark, 1999). At these temperatures, not only the DNA double helix unwind, but it also induces various chemical modifications that ...
Fluorescence-Activated Flow Sorting of Metaphase Chromosomes
... The IMR-32 cells used for the present experiments contain a modal number of 48 chromosomes. Approximately 70% of cells have only-one normal chromosome 1 plus two apparently identical abnormal chromosomes 1 (Fig. 1). These abnormal chromosomes result from insertion of a segment of homogeneously stain ...
... The IMR-32 cells used for the present experiments contain a modal number of 48 chromosomes. Approximately 70% of cells have only-one normal chromosome 1 plus two apparently identical abnormal chromosomes 1 (Fig. 1). These abnormal chromosomes result from insertion of a segment of homogeneously stain ...
BYB2 - TheAllPapers
... Callus tissue develops into either shoots or roots depending on the relative concentration of the plant growth regulators used. Use your knowledge of genes to suggest how these plant growth regulators determine the type of plant tissue formed. ...
... Callus tissue develops into either shoots or roots depending on the relative concentration of the plant growth regulators used. Use your knowledge of genes to suggest how these plant growth regulators determine the type of plant tissue formed. ...
Monitoring endangered freshwater biodiversity using environmental
... When the animals have been removed from the containers, only DNA degradation occurs: xðtÞ ¼ xðtR Þ expð$cðt $ tR ÞÞ ...
... When the animals have been removed from the containers, only DNA degradation occurs: xðtÞ ¼ xðtR Þ expð$cðt $ tR ÞÞ ...
BIOL4 - gale-force-glyn
... leaf palisade cells? What is the structure of a chloroplast and how is it related to its function? What is the plant cell wall composed of and what is its function? How do plant cells differ from animal cells? ...
... leaf palisade cells? What is the structure of a chloroplast and how is it related to its function? What is the plant cell wall composed of and what is its function? How do plant cells differ from animal cells? ...
DNA Denaturing through UV-C Photon Dissipation: A
... small prevalence of right over left handed circularly polarized submarine light in the late afternoon (Angel et al., 1972; Wolstencroft, 2004) when surface water temperatures are highest and thus more conducive to denaturing. The UVTAR mechanism also provides an explanation for the beginnings of inf ...
... small prevalence of right over left handed circularly polarized submarine light in the late afternoon (Angel et al., 1972; Wolstencroft, 2004) when surface water temperatures are highest and thus more conducive to denaturing. The UVTAR mechanism also provides an explanation for the beginnings of inf ...
Electroosmotic screening of the DNA charge in a
... 5 ns was required to balance the effective driving force by the force of the spring. Each simulation was continued for an additional 15 ns to determine the radial distribution of ions, the velocity profile of water, and the average stall force F . In a nanopore, the radial density of counterions dep ...
... 5 ns was required to balance the effective driving force by the force of the spring. Each simulation was continued for an additional 15 ns to determine the radial distribution of ions, the velocity profile of water, and the average stall force F . In a nanopore, the radial density of counterions dep ...
in Power-Point Format
... without help from other proteins • Inverted repeat leads transcript to hairpin structure • T-rich region in nontemplate strand produces string of weak rU-dA base pairs holding transcript to template ...
... without help from other proteins • Inverted repeat leads transcript to hairpin structure • T-rich region in nontemplate strand produces string of weak rU-dA base pairs holding transcript to template ...
Genomics I
... adenosine 5′ phosphosulfate, and luciferin. Sequentially flow solutions containing A, T, G, or C into the wells. In the example below, T has been added to the wells. PPi (pyrophosphate) is released when T is incorporated into the growing strand. T T C AT GCA Primer Adenosine ...
... adenosine 5′ phosphosulfate, and luciferin. Sequentially flow solutions containing A, T, G, or C into the wells. In the example below, T has been added to the wells. PPi (pyrophosphate) is released when T is incorporated into the growing strand. T T C AT GCA Primer Adenosine ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.