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FLISIAK et al, Hepatology 2009
FLISIAK et al, Hepatology 2009

... inhibitory effect of autophagy on innate immune response CARREIRA et al, Autophagy 2010 ...
Similarities and Differences between RNA and DNA Recognition by
Similarities and Differences between RNA and DNA Recognition by

... The extensive hydrogen bonding in shape complementarity between the major groove of B-DNA and the surfaces of many of the sequencespecific DNA-binding proteins as a source of recognition has been extensively documented from high-resolution crystal structures and a few NMR structures of DNA complexes ...
Gene mutation
Gene mutation

Gene Transfer
Gene Transfer

... have the capacity to be transformed, at various levels of efficiency, as reviewed by Lorenz and Wachernagel (1994). In the unlikely event that a plasmid were released from a maize cell as described above, it could only replicate in E. coli and some of its close relatives which can utilize the specif ...
Sonogenetics: A Breakthrough in Prenatal Diagnosis
Sonogenetics: A Breakthrough in Prenatal Diagnosis

... of a chromosomal etiology allows the clinician to shift the diagnostic focus onto other etiologies, such as Mendelian disorders and environmental insults. 5 The subsequent development and application of microarray-based assays have established the importance of copy number variants (CNVs) as a subst ...
Case Study: Visualization of annotated DNA sequences
Case Study: Visualization of annotated DNA sequences

... tation type may dictate the number of regions. The extent of an annotation α is the region [min..max] where min is the minimum of all start positions and max the maximum of all end positions of the regions of α. properties is a set of name-value pairs representing properties that are not contained i ...
UltraClean 15 DNA Purification Kit
UltraClean 15 DNA Purification Kit

... What’s happening: ULTRA SALT is sodium iodide (a chaotropic salt) that breaks hydrogen bonds that hold solidified agarose together. Once the gel is melted, it will not re-solidify. ...
Polymorphisms and Genomic Organization of Repetitive
Polymorphisms and Genomic Organization of Repetitive

NPA White Paper: DNA Barcoding for Botanical Authentication Issue
NPA White Paper: DNA Barcoding for Botanical Authentication Issue

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File - Integrated Science

... dsRNA into C. elegans which led to an efficient sequencespecific silencing and coined the term "RNA Interference". ...
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Week of 09/04

Biology, 8th Edition
Biology, 8th Edition

... from bacteria, known as restriction enzymes, to cut DNA molecules only in specific places. Restriction enzymes enable researchers to cut DNA into manageable segments. Each fragment is then incorporated into a suitable vector molecule, a carrier capable of transporting the DNA fragment into a cell. Ba ...
CtrA mediates a DNA replication checkpoint that prevents cell
CtrA mediates a DNA replication checkpoint that prevents cell

... The stalked cell re-enters S phase immediately after cell division and grows into an asymmetric pre-divisional cell. In contrast, the swarmer cell must differentiate into a stalked cell before entering S phase. The establishment of cellular asymmetry prior to cell division is tightly coupled to prog ...
Supplementary information PDF
Supplementary information PDF

Document
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... formed when TFIIF was absent, Thus, TFIIF appears to bring polymerase II to the complex. The lanes on the right show what happened when Reinberg and colleagues left out one factor at a time. In lane 13, without TFIID, no complexes formed at all Lane 14 shows that the DA complex, but no tubers, forme ...
The Effects of Plasmids of Genotype and Phenotype
The Effects of Plasmids of Genotype and Phenotype

... you can readily appreciate how this type of gene can cause serious medical problems when it occurs in pathogenic bacteria. For this reason, the plasmids such as pUC 18 which are used in recombinant DNA experiments were designed so that they cannot be exchanged with other bacteria except by special t ...
Chapter 15: Genes and How They Work
Chapter 15: Genes and How They Work

... In both bacteria and eukaryotes, the polymerase adds ribonucleotides to the growing 3′ end of an RNA chain. No primer is needed, and synthesis proceeds in the 5′ → 3′ direction. Bacteria contain only one RNA polymerase enzyme, while eukaryotes have three different RNA polymerases: RNA polymerase I s ...
Determination of the Binding Site-Size of the Protein
Determination of the Binding Site-Size of the Protein

... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
RNA/DNA catalysts
RNA/DNA catalysts

Note 20 - South Tuen Mun Government Secondary School
Note 20 - South Tuen Mun Government Secondary School

... virus DNA (viral DNA), bacterial plasmid. In this example: plasmid acts as the vector. A plasmid is a smaller circular / ring of extrachromosomal DNA in bacteria. Restriction enzyme [endonuclease / restriction eodonuclease]  recognizes specific base sequence and cut the DNA at specific site ...
DNA and RNA extraction
DNA and RNA extraction

... it should be noted that some enzymes cleave Physcomitrella DNA to a greater extent than others. This probably relates to the extent and distribution of methylation of the moss genome. - This has been discussed by Krogan & Ashton (1999) who demonstrated that enzymes with recognition sites subject to ...
A-level Human Biology Question paper Unit 3 - Pathogens
A-level Human Biology Question paper Unit 3 - Pathogens

... Scientists have discovered a new technique for detecting disease-causing microorganisms. This technique could lead to faster and cheaper diagnosis of diseases such as salmonella food poisoning. The scientists point out, however, that this new technique is no substitute for following the basic rules ...
Similarities and Differences Between mRNA and tRNA
Similarities and Differences Between mRNA and tRNA

... shapes of tRNA  use paper models to show how tRNA molecules bring specific amino acid molecules to the ribosome where building of proteins occurs.  Learn to transcribe a DNA code to a mRNA message and translate the mRNA to the tRNA-amino acid code  Study the molecular basis for mutations Part A R ...
The energetic basis of the DNA double helix: a
The energetic basis of the DNA double helix: a

... and widely accepted, that the enthalpy of DNA duplex dissociation/association does not depend on temperature (10–13). On the other hand, the enthalpy of DNA dissociation at elevated temperatures, determined by DSC, was found to be in conflict with the enthalpy of association of the complementary str ...
Gel Electrophoresis
Gel Electrophoresis

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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