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Chapter 12-1 - DNA
Chapter 12-1 - DNA

... bacteria together and let virus work • After a time, put mixture into a blender Purpose was to shake viral coats off of the bacteria ...
Restriction Enzymes
Restriction Enzymes

DNA and Its Role in Heredity Reading Assignments
DNA and Its Role in Heredity Reading Assignments

... eucaryotes y have many y (10 ( 2 to 103)). • Replication for each proceeds in both directions from an origin of replication. ...
Structure of a six-finger transcription factor IIIA complex
Structure of a six-finger transcription factor IIIA complex

Jeopardy, cells part 2 review
Jeopardy, cells part 2 review

... Which of the following may alter mitosis and cause mutations of DNA. A)medications B) chemical exposture C) radiation D) all of the above ...
Ch. 14 - Crestwood Local Schools
Ch. 14 - Crestwood Local Schools

... toward the replication fork in the 5’-->3’ direction. ...
biotechnology
biotechnology

... separated from their respective fusion proteins by treatment with carboxypeptidase and trypsin. Statement B: After the separation of A and B chains from fusion proteins, the two chains are bonded by disulphide bonds by treating them with sodium disulphonate. ...
DNA Replication
DNA Replication

... – DNA synthesis begins at a site termed the origin of replication • Each bacterial chromosome has only one ...
Figure 11.7
Figure 11.7

... – DNA synthesis begins at a site termed the origin of replication • Each bacterial chromosome has only one ...
DNA
DNA

... helping to maximize the odds that each molecule is a perfect copy of the original DNA DNA ligase – connects together the Okazaki fragments on the lagging strand ...
BIOL 112 – Principles of Zoology
BIOL 112 – Principles of Zoology

... will pair w/A leading to a GC to AT transition Oxidative damage – superoxide radicals (byproducts of metabolism) alter bases to cause mispairing… 8oxidG or GO pairs with A ...
THREE POSSIBILE MODELS FOR REPLICATION
THREE POSSIBILE MODELS FOR REPLICATION

Test # 1. Which of the following is not an electron acceptor or carrier?
Test # 1. Which of the following is not an electron acceptor or carrier?

... In chromosomal replication, one DNA strand is built continuously, while the other strand is built in pieces, called Okazaki fragments. b) Most cells can divide an infinite number of times. c) An RNA primer is required in chromosomal replication because DNA polymerase will not bind to a single stand ...
THREE POSSIBILE MODELS FOR REPLICATION
THREE POSSIBILE MODELS FOR REPLICATION

...  RNA POLYMERASE binds to DNA at region called PROMOTER LIKE DNA POLYMERASE: can only attach nucleotides in 5’ → 3’ direction; UNLIKE DNA POLYMERASE: can start a chain from scratch; no primer needed  In eukaryotes: TRANSCRIPTION FACTORS & TATA BOXES help position/bind to correct spot  RNA POLYMERA ...
single-nucleotide polymorphism
single-nucleotide polymorphism

... A single-nucleotide polymorphism • A single-nucleotide polymorphism (SNP, pronounced snip) is a DNA sequence variation occurring when a single nucleotide — A, T, C, or G — in the genome (or other shared sequence) differs between members of a species (or between paired chromosomes in an individual) ...
Lecture 2 Nucleic Acid Structure
Lecture 2 Nucleic Acid Structure

... 1 A2 of hydrophobic surface ca. 24 cal/mol stabilization when removed from H2O -Hydrophobic Effect can be relatively non-specific when molecules bind to protein or DNA ...
Protein Synthesis Overview IP
Protein Synthesis Overview IP

... the proteins your body makes. Proteins, in turn, determine the structure and function of all your cells. What determines a protein’s structure? It begins with the sequence of amino acids that make up the protein. Instructions for making proteins with the correct sequence of amino acids are encoded i ...
DNA Deoxyribonucleic Acid
DNA Deoxyribonucleic Acid

Worksheet - Oregon State University
Worksheet - Oregon State University

... 6.    What  molecule  ends  polypeptide  synthesis  by  recognizing  the  stop  codon?   ...
Document
Document

...  Carries out protein synthesis  Differences from DNA:  different sugar (ribose)  single strand  different base  no thymine  URACIL instead ...
PDF file - Gupta Lab
PDF file - Gupta Lab

... ◦ Ionizing radiation can penetrate cells and create ions in the cell contents. These can cause permanent alteration in DNA, that is mutations. ◦ The somewhat shorter Ultraviolet rays(260 nanometer)and Gamma and x-rays are electromagnetic(like light) that can be absorbed by DNA. Ultraviolet light is ...
RNA.transcription.translation
RNA.transcription.translation

...  Carries out protein synthesis  Differences from DNA:  different sugar (ribose)  single strand  different base  no thymine  URACIL instead ...
DNA - heredity2
DNA - heredity2

... • Affects the haemoglobin • Most common form is caused by a recessive trait on chromosome 11, a single base change (T  A) which makes the 6th codon Val not Glu on the β-globin polypeptide • Causes RBCs to form a sickle shape when the concentration of oxygen is low ...
Document
Document

... Ligase – brings together Okazaki fragments DNA polymerases add nucleotides only to the free 3end of a growing strand; therefore, a new DNA strand can elongate only in the 5to 3direction ...
III. Biotechnology
III. Biotechnology

... 4. DNA Sequencing a) A piece of DNA with an unknown sequence is placed in 4 Eppendorf tube containing primase, nucleotides, and DNA polymerase. Dideoxynucleotides (ddA, ddT, ddC & ddG) are added to different tubes. b) In the tube that contain ddT, sometimes deoxythymine will be added, but sometimes ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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