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Glossary of Bacterial Genetics
Glossary of Bacterial Genetics

... a prokaryotic, microscopic, single-celled organism with a cell wall that increases by binary fission ...
ID of Genetic Material
ID of Genetic Material

... enzyme-catalyzed reactions. It begins with the attachment of a particular enzyme to a special site on the DNA molecule. This causes the two strands of DNA to unwind in that area. It is thought that only one of the two strands of DNA serves as a template for RNA synthesis. The RNA nucleotides (ribonu ...
Biology 3 Questions  1. Which is found in prokaryotic cell? (Cell)
Biology 3 Questions 1. Which is found in prokaryotic cell? (Cell)

... d) In yeast growing anaerobically, pyruvate is converted to ethanol. 35. Consider a DNA sample, which is true? (DNA/RNA) a) A=C b) C=U c) A+T=C+G d) C+T=A+G 36. Consider 5’-AATCGACCT-3’, this deoxyribnucleotide polymer could form a double stranded structure with what? (DNA/RNA) 37. Steroid hormones ...
Real-time monitoring of branched rolling
Real-time monitoring of branched rolling

Slayt 1
Slayt 1

... into the bacterial chromosome by a crossover, similar to the conversion of an F plasmid into an Hfr. Once incorporated into the chromosome, the lambda DNA becomes quiescent: its genes are not expressed and it remains a passive element on the chromosome, being replicated along with the rest of the ch ...
Transcription in Bacteria
Transcription in Bacteria

... After about 9-12 nt of RNA have been synthesized, the initiation complex enters the elongation stage. As RNA polymerase moves during elongation, it holds the DNA strands apart, forming a transcription “bubble.” The moving polymerase protects a “footprint” of ~30 bp along the DNA against nuclease dig ...
DNA Notes (in pdf) - Nutley Public Schools
DNA Notes (in pdf) - Nutley Public Schools

... •  All cells come from the division of pre-existing cells. •  CELL DIVISION - process where a cell splits to produce a new cell •  Before a cell divides, all the genetic material (DNA) must be copied. •  This will allow for both cells (2 after cell splits) to have a complete copy of the genetic mate ...
Exam Procedures: this isBMB 526 Exam #1 11/5/12 this is form A
Exam Procedures: this isBMB 526 Exam #1 11/5/12 this is form A

... eukaryotic DNA replication? A. DNA polymerase α(alpha) B. DNA polymerase β (beta) C. RNA polymerase γ (gamma) D. DNA polymerase ε (epsilon) E. telomerase 9. Some patients with familial hypercholesterolemia produce a truncated form of the LDL receptor which lacks three of the five domains of the prot ...
Biotechnology
Biotechnology

... Bacteria  Bacteria review one-celled prokaryotes  reproduce by mitosis ...
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A DNA

... Genetic material in prokaryotes  1 (usually) chromosome  Circular (most) chromosome  Supercoiled DNA located in nucleoid region ...
From Gene To You
From Gene To You

...  Nitrogen Rings  Purines are double ring bases Adenine, ...
From RNA to protein
From RNA to protein

... When DNA base sequences are cited, by convention it is the sequence of the non-template (sense, coding) strand that is given, even though the RNA is transcribed from the template strand. The following Table shows highlighted sequences from the HvCBF2A gene and their interpretation. ...
From RNA to protein
From RNA to protein

... When DNA base sequences are cited, by convention it is the sequence of the non-template (sense, coding) strand that is given, even though the RNA is transcribed from the template strand. The following Table shows highlighted sequences from the HvCBF2A gene and their interpretation. ...
Nucleic Acids - Biology Junction
Nucleic Acids - Biology Junction

...  3 H bonds Matching bases? Why is this important? ...
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DNA

... restriction enzymes and TAQ polymerase. • Work on ice to slow down enzymatic processes. • Wear gloves to protect your samples from you!! • Autoclave all solutions and store in fridge (except SDS and ...
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DNA AP Bioloy

Chapter 24: Genes and Chromosomes
Chapter 24: Genes and Chromosomes

... The DNA of virtually every cell is underwound (i.e., negatively supercoiled) relative to B-form DNA. In bacteria, an enzyme called (a) ____________ introduces negative supertwists into DNA. This enzyme is classified as a type (b) ____________, which affects the linking number in steps of (c) _______ ...
Bell Work: What does DNA stand for?
Bell Work: What does DNA stand for?

... radioactive sulfur atoms in their protein.  They then used a  blender, to separate the bacteria from the phages that  remained outside the bacteria.  None of them showed  evidence of radioactivity. Experiment 2­ Bacteria tagged with radioactive phosphorus  were put through the same test.  This time  ...
Unit 08 Notes - Pierce College
Unit 08 Notes - Pierce College

... 1) G1 phase. The cell has just divided and it is actively growing, meaning it is synthesizing polypeptides. During the G1 phase, the cell decides whether it will divide or not, as influenced by growth factors secreted from tissues adjacent to the cell. If cell is triggered to divide, it will enter t ...
Ch.16 17 Study Guide
Ch.16 17 Study Guide

... experiments of Matthew Meselson and Franklin Stahl. 6. Describe the process of DNA replication, including the role of the origins of replication and replication forks. 7. Explain the role of DNA polymerases in replication. 8. Explain what energy source drives the polymerization of DNA. 9. Distinguis ...
Slide 1
Slide 1

... • Compare graphs for functional and non functional siRNA • For these two sets of siRNA, compute graph properties that reflect sequence structure. ...
Document
Document

... • Cause of using RNA bases: RNA molecules provide the link between DNA and actual protein ...
Foundations in Microbiology
Foundations in Microbiology

... removal of genetic material from one organism and combining it with that of a different organism – Objective of recombinant technology is cloning which requires that the desired donor gene be selected, excised by restriction endonucleases, and isolated – The gene is inserted into a vector (plasmid, ...
Review - UCR Class!
Review - UCR Class!

... • What removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3; end of the Okazaki fragments? ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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