BIO305 - National Open University of Nigeria
... localisation of proteins to subcellular organelles. In this way, it is possible to study genetic differences between organisms or individuals. Hybridisation can be achieved by southern or northern blotting. Southern blotting is a method for probing for the presence of a specific DNA sequence within ...
... localisation of proteins to subcellular organelles. In this way, it is possible to study genetic differences between organisms or individuals. Hybridisation can be achieved by southern or northern blotting. Southern blotting is a method for probing for the presence of a specific DNA sequence within ...
Lesson
... Traits are observable characteristics. While each of us shares some of our traits with many other people, the combination of all our individual traits is what makes us unique. All the traits in your body are determined by bits of DNA called genes. Hundreds of genes together form chromosomes, which a ...
... Traits are observable characteristics. While each of us shares some of our traits with many other people, the combination of all our individual traits is what makes us unique. All the traits in your body are determined by bits of DNA called genes. Hundreds of genes together form chromosomes, which a ...
Casework Genetics Uses Illumina Technologies to Decipher
... Application Note: DNA Analysis Expanding Forensic Analysis Applications Confident in their ability to produce high-quality data and compare DNA from multiple contributors to persons of interest, Casework Genetics has also begun looking at more complex problems related to mixtures. For example, comp ...
... Application Note: DNA Analysis Expanding Forensic Analysis Applications Confident in their ability to produce high-quality data and compare DNA from multiple contributors to persons of interest, Casework Genetics has also begun looking at more complex problems related to mixtures. For example, comp ...
Sample pages 1 PDF
... uptake DNA by horizontal DNA transfer. However, some bacteria such as Pseudomonas species which are not naturally competent must use more complex strategies such as conjugation to uptake DNA. Bacterial conjugation (described Chapter 4) is very efficient in Pseudomonas but requires the presence of a ...
... uptake DNA by horizontal DNA transfer. However, some bacteria such as Pseudomonas species which are not naturally competent must use more complex strategies such as conjugation to uptake DNA. Bacterial conjugation (described Chapter 4) is very efficient in Pseudomonas but requires the presence of a ...
PPT File
... An attractive strategy for single-molecule DNA sequencing is to pass single-stranded DNA through a nanopore in a graphene monolayer. Here, the rings of carbon atoms in the graphene are depicted as hexagons, and the diameter of the nanopore is about 1.5 nm, corresponding to about 35 hexagonal units. ...
... An attractive strategy for single-molecule DNA sequencing is to pass single-stranded DNA through a nanopore in a graphene monolayer. Here, the rings of carbon atoms in the graphene are depicted as hexagons, and the diameter of the nanopore is about 1.5 nm, corresponding to about 35 hexagonal units. ...
Regulating Gene Expression
... These are considered non-living b/c they have no metabolism, homeostasis, growth, and require a host cell to carry out their functions Are extraordinarily small, since they are active inside of cells. They can contain traditional, double-stranded DNA, single-stranded DNA, or even RNA Recall ...
... These are considered non-living b/c they have no metabolism, homeostasis, growth, and require a host cell to carry out their functions Are extraordinarily small, since they are active inside of cells. They can contain traditional, double-stranded DNA, single-stranded DNA, or even RNA Recall ...
Where Is DNA Found?
... Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. Commercial kits are now available for easy PCR reaction setup and amplification. Contaminant DNA, such as from fungal and bacterial sources, will not amplify because human-specific primers ...
... Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. Commercial kits are now available for easy PCR reaction setup and amplification. Contaminant DNA, such as from fungal and bacterial sources, will not amplify because human-specific primers ...
Organization of DNA replication origins in the fission yeast genome
... Only nascent strand DNA is protected by its RNA primer from the 5⬘ to 3⬘ λ-exonuclease activity, and this is used as a template to extend a labelled oligonucleotide to the junction with the RNA primer. The smallest detectable fragment indicates the position of the first deoxyribonucleotide synthesiz ...
... Only nascent strand DNA is protected by its RNA primer from the 5⬘ to 3⬘ λ-exonuclease activity, and this is used as a template to extend a labelled oligonucleotide to the junction with the RNA primer. The smallest detectable fragment indicates the position of the first deoxyribonucleotide synthesiz ...
Recombinases
... complementary interfaces to form a stable synaptic complex (149, 150). Following recombination, the conformations switch to the attL and attR specificities, the interface complementarity breaks down, and the complex dissociates into the separate integrase-bound attL and attR sites. Because these pha ...
... complementary interfaces to form a stable synaptic complex (149, 150). Following recombination, the conformations switch to the attL and attR specificities, the interface complementarity breaks down, and the complex dissociates into the separate integrase-bound attL and attR sites. Because these pha ...
Therapeutic Targeting of the DNA Mismatch Repair Pathway
... does this task by recognizing distortions in the DNA double helix structure caused by mismatched bases (12). MutS initially binds double-stranded DNA at the site of a mismatch and then recruits MutL. MutL seems to act as the mediator for a series of subsequent protein interactions that facilitate MM ...
... does this task by recognizing distortions in the DNA double helix structure caused by mismatched bases (12). MutS initially binds double-stranded DNA at the site of a mismatch and then recruits MutL. MutL seems to act as the mediator for a series of subsequent protein interactions that facilitate MM ...
Recombinant DNA Technology
... Another example of a typical E. coli cloning vector is pUC19 (2,686-bp). The pUC19 plasmid features: a. High copy number in E. coli, with nearly a hundred copies per cell, provides a good yield of cloned DNA. b. Its selectable marker is ampR. c. It has a cluster of unique restriction sites, called t ...
... Another example of a typical E. coli cloning vector is pUC19 (2,686-bp). The pUC19 plasmid features: a. High copy number in E. coli, with nearly a hundred copies per cell, provides a good yield of cloned DNA. b. Its selectable marker is ampR. c. It has a cluster of unique restriction sites, called t ...
Lesson Overview
... strands of DNA are “antiparallel”— they run in opposite directions. This arrangement enables the nitrogenous bases on both strands to come into contact at the center of the molecule. ...
... strands of DNA are “antiparallel”— they run in opposite directions. This arrangement enables the nitrogenous bases on both strands to come into contact at the center of the molecule. ...
Recombinant DNA Technology
... Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker. ...
... Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker. ...
XRCC1 (X-Ray Repair Cross Complementing
... XRCC1 (X-Ray Repair Cross Complementing Protein1) Ab-1 (Clone 33-2-5) ...
... XRCC1 (X-Ray Repair Cross Complementing Protein1) Ab-1 (Clone 33-2-5) ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.