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MENDEL MEETS CSI: Forensic Genotyping as a Method To Teach
MENDEL MEETS CSI: Forensic Genotyping as a Method To Teach

... To extract DNA from buccal cells, sterile cotton swabs, a boiling water bath, and a micro-centrifuge are needed. The cell lysate obtained is crude and contains heavy metal ions that can interfere with PCR amplification by either inhibiting DNA polymerase or by acting as cofactors for nucleases that ...
GENETIC ENGINEERING - PLASMIDS, EPISOMES
GENETIC ENGINEERING - PLASMIDS, EPISOMES

... which can infect a cell and insert themselves into its DNA. With this knowledge, scientists started to build vectors which incorporated genes of their choosing and used the new vectors to insert these genes into the DNA of living organisms. Genetic engineers believe they can improve the foods we eat ...
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GENE TECHNOLOGY - mf011

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In depth: the role of the function of DNA sequence before and after

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Watermarking sexually reproducing diploid organisms

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C2005/F2401 `07 -- Lecture 16 -- Last Edited

... chromosome. Therefore recipient can get a fragment (from a copy of part of the donor's chromosome) or a plasmid. See Becker fig. 20-21 (18-21). 5. Cell to cell contact is required. Conjugation, unlike transformation, requires cell-cell contact and the DNA (copy) is passed across a bridge that forms ...
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... Knowledge of the DNA sequence to be amplified is used to design two synthetic DNA oligonucleotides, each complementary to the sequence on one strand of the DNA double helix at opposite ends of the region to be amplified. These oligonucleotides serve as primers for in vitro DNA synthesis, which is pe ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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