Yeast whole-genome analysis of conserved regulatory motifs
... • Enhancer networks: Regulator enhancer target gene • Ex1: Oct4 predicted activator of embryonic stem (ES) cells • Ex2: Gfi1 repressor of K562/GM cells ...
... • Enhancer networks: Regulator enhancer target gene • Ex1: Oct4 predicted activator of embryonic stem (ES) cells • Ex2: Gfi1 repressor of K562/GM cells ...
3: The Technologies
... Human proteins may be isolated directly from cultured cells. Cell cultures can also be the source of the genetic material needed to apply recombinant DNA technology in further studying a problem. As will be described later in this chapter, cultured human cells, both primary and established, play an ...
... Human proteins may be isolated directly from cultured cells. Cell cultures can also be the source of the genetic material needed to apply recombinant DNA technology in further studying a problem. As will be described later in this chapter, cultured human cells, both primary and established, play an ...
DNA cytosine methylation in plant development
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
S1 Table. Experimental evidence supporting interactions between
... controls the rate and orientation of vascular cell division. Development. 137:767-774. Hardtke, C.S., and T. Berleth. 1998. The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. 1405-1411 pp. Hass, C., J. Lohrmann, V. Albrecht, U. Sw ...
... controls the rate and orientation of vascular cell division. Development. 137:767-774. Hardtke, C.S., and T. Berleth. 1998. The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. 1405-1411 pp. Hass, C., J. Lohrmann, V. Albrecht, U. Sw ...
Apoptosis - Learning
... Sixteen different cyclins have been identified, but like the CDK’s, not all are cell cycle related. Like their respective protein kinases, the cyclins act at very specific points in the cell cycle. The D cyclins D1 D2 & D3 bind to CDK4 and CDK6 and it is this step that is crucial for entry into G1. ...
... Sixteen different cyclins have been identified, but like the CDK’s, not all are cell cycle related. Like their respective protein kinases, the cyclins act at very specific points in the cell cycle. The D cyclins D1 D2 & D3 bind to CDK4 and CDK6 and it is this step that is crucial for entry into G1. ...
Differentiation of Cardiac Myocytes after Mitogen Withdrawal
... By days 2 and 3, the contractile myocytes developed a characteristic morphology consisting of a coarse granular cytoplasm with dense inclusions and a dense round nucleus. By the criteria of morphology and spontaneous contraction, the ventricular myocytes were readily distinguished from nonmuscle cel ...
... By days 2 and 3, the contractile myocytes developed a characteristic morphology consisting of a coarse granular cytoplasm with dense inclusions and a dense round nucleus. By the criteria of morphology and spontaneous contraction, the ventricular myocytes were readily distinguished from nonmuscle cel ...
Return to the RNAi world: rethinking gene expression and
... remarkably stable differentiation events can be maintained for the entire life of an organism without any underlying changes in the DNA sequence. The germline cells, which in C. elegans inherit PIE-1 protein, are the only cells that retain the potential to launch the developmental program again in t ...
... remarkably stable differentiation events can be maintained for the entire life of an organism without any underlying changes in the DNA sequence. The germline cells, which in C. elegans inherit PIE-1 protein, are the only cells that retain the potential to launch the developmental program again in t ...
Eye Development
... The transcription factor Pax6 is possibly required for maintenance of lens competence in mice. Expressed from early stages in head ectoderm in a region which overlaps, but is wider than the area fated to form the lens. In chimeric mice that were a mixture of Pax6+/+ and Pax6-/- cells, the mutant cel ...
... The transcription factor Pax6 is possibly required for maintenance of lens competence in mice. Expressed from early stages in head ectoderm in a region which overlaps, but is wider than the area fated to form the lens. In chimeric mice that were a mixture of Pax6+/+ and Pax6-/- cells, the mutant cel ...
vHnf1 mutant embryos lack visceral endoderm - Development
... (Cereghini et al., 1988), vHNF1 is a member of a complex regulatory network composed of a heterogeneous class of transcription factors including HNF-4, HNF-3α, β and γ (Cereghini, 1996). The temporal and spatial expression pattern of vHnf1 is consistent with the hypothesis that it plays a role in ea ...
... (Cereghini et al., 1988), vHNF1 is a member of a complex regulatory network composed of a heterogeneous class of transcription factors including HNF-4, HNF-3α, β and γ (Cereghini, 1996). The temporal and spatial expression pattern of vHnf1 is consistent with the hypothesis that it plays a role in ea ...
Parental Methamphetamine Exposure Affects Offspring`s Behavior and
... Maternal influence on the phenotype (independent of dam treatment) also sexually dimorphic Fear conditioning: Maternal influence on the phenotype dependent on dam treatment (“saline rescue”) Open field: Maternally influenced phenotye (dependent on dam treatment (“METH induced”) Light/dark bo ...
... Maternal influence on the phenotype (independent of dam treatment) also sexually dimorphic Fear conditioning: Maternal influence on the phenotype dependent on dam treatment (“saline rescue”) Open field: Maternally influenced phenotye (dependent on dam treatment (“METH induced”) Light/dark bo ...
D9-Tetrahydrocannabinol inhibits cell cycle progression by
... activity with effect on cell cycle progression. We found increases in the percentage of cells sequestered in the G0-G1 compartment from 59% in control cells to 76% in U87-MG during induction of D9-THC and statistical significant elevation from 55% in control cells to 73% post treatment with D9-THC i ...
... activity with effect on cell cycle progression. We found increases in the percentage of cells sequestered in the G0-G1 compartment from 59% in control cells to 76% in U87-MG during induction of D9-THC and statistical significant elevation from 55% in control cells to 73% post treatment with D9-THC i ...
DNA methylation involved in proline accumulation in - Funpec-RP
... 1998; Nanjo et al., 2003; Verbruggen and Hermans, 2008). Proline is synthesized via the glutamate pathway via 2 key enzymes, Δ1-pyrroline-5-carboxylate synthetase (P5CS, EC 1.5.1.12) and Δ1-pyrroline-5-carboxylate reductase (P5CR, EC 1.5.1.2) (Hu et al., 1992; Verbruggen and Hermans, 2008), whereas ...
... 1998; Nanjo et al., 2003; Verbruggen and Hermans, 2008). Proline is synthesized via the glutamate pathway via 2 key enzymes, Δ1-pyrroline-5-carboxylate synthetase (P5CS, EC 1.5.1.12) and Δ1-pyrroline-5-carboxylate reductase (P5CR, EC 1.5.1.2) (Hu et al., 1992; Verbruggen and Hermans, 2008), whereas ...
Scenario 2 - people.vcu.edu
... Andrey Matveyev, Kathryn T Young, Andrew Meng, and Jeff Elhai Dept. of Biology, University of Richmond, Richmond VA USA From the characterization of enzyme activities and the analysis of genomic sequences, the complement of DNA methyltransferases (MTases) possessed by the cyanobacterium Anabaena PCC ...
... Andrey Matveyev, Kathryn T Young, Andrew Meng, and Jeff Elhai Dept. of Biology, University of Richmond, Richmond VA USA From the characterization of enzyme activities and the analysis of genomic sequences, the complement of DNA methyltransferases (MTases) possessed by the cyanobacterium Anabaena PCC ...
Biochemical studies of carbohydrate blood group antigens
... deletion of glycosyltransferase genes is a powerful technique to study the biological function of selected carbohydrate antigens. However, shifting the equilibrium between competing glycosyltransferases might lead to unexpected phenotypic effects, such as accumulation of other carbohydrate antigens ...
... deletion of glycosyltransferase genes is a powerful technique to study the biological function of selected carbohydrate antigens. However, shifting the equilibrium between competing glycosyltransferases might lead to unexpected phenotypic effects, such as accumulation of other carbohydrate antigens ...
A1988L880000001
... a means for the removal of cell walls from cell surfaces and for the detection of regenerating cell walls on the protoplast surface. Although electron microscopy can be used for the same purpose, it is time-consuming and laborious, a sharp contrast to the procedure that uses this fluorescent dye. No ...
... a means for the removal of cell walls from cell surfaces and for the detection of regenerating cell walls on the protoplast surface. Although electron microscopy can be used for the same purpose, it is time-consuming and laborious, a sharp contrast to the procedure that uses this fluorescent dye. No ...
Probing Lymphocyte Biology by Genomic-Scale Gene Expression Analysis.
... were found to be maximally repressed at 3 and 6 hr following activation and then return to near-resting levels after 24 hr. This suggests that a window of time exists early during T cell activation in which the trafficking of the cell is inhibited. A second repressed gene that may illuminate T cell ...
... were found to be maximally repressed at 3 and 6 hr following activation and then return to near-resting levels after 24 hr. This suggests that a window of time exists early during T cell activation in which the trafficking of the cell is inhibited. A second repressed gene that may illuminate T cell ...
Evolution
... is concluded that PLT promoter activity leads to protein gradients with maximum expression in the stem cell niche. PLT1 and PLT2 expression maxima broadly encompass the niche, whereas PLT3 and BBM are more restricted. ...
... is concluded that PLT promoter activity leads to protein gradients with maximum expression in the stem cell niche. PLT1 and PLT2 expression maxima broadly encompass the niche, whereas PLT3 and BBM are more restricted. ...
Name:______ Period
... b. What process is used to create un-identical haploid sex cells? _________________ ...
... b. What process is used to create un-identical haploid sex cells? _________________ ...
Accepted Version - CSIRO Research Publications Repository
... (Figure 1). However, in a self-pollinated ddm1 mutant, the BNS gene is methylated and stochastically silenced (Figure 1), indicating that bns is a loss of function epigenetic mutant. The BNS gene is flanked by a LINE (Long interspersed repeated element) sequence in a tail-to-tail orientation, and in ...
... (Figure 1). However, in a self-pollinated ddm1 mutant, the BNS gene is methylated and stochastically silenced (Figure 1), indicating that bns is a loss of function epigenetic mutant. The BNS gene is flanked by a LINE (Long interspersed repeated element) sequence in a tail-to-tail orientation, and in ...
Exam 1 Q2 Review Sheet
... 23. Compare the extended form of DNA to the condensed form. At what stage in the cell cycle would you find each? 24. Know the difference between the terms DNA, chromosome, and chromatin. 25. Describe the chromosomes found in the human nucleus (how many, which are homologous, autosomes, sex chromosom ...
... 23. Compare the extended form of DNA to the condensed form. At what stage in the cell cycle would you find each? 24. Know the difference between the terms DNA, chromosome, and chromatin. 25. Describe the chromosomes found in the human nucleus (how many, which are homologous, autosomes, sex chromosom ...
D melanogaster - GEP Community Server
... Check out your gene on FlyBase – what is the pattern of expression in D. melanogaster? Has a function been described? Many dot chromosome genes are expressed at a high level - how can this occur in a heterochromatic domain? ...
... Check out your gene on FlyBase – what is the pattern of expression in D. melanogaster? Has a function been described? Many dot chromosome genes are expressed at a high level - how can this occur in a heterochromatic domain? ...
Molecular Cell Biology - Biomedical Informatics
... Characteristic features of gene regulation mechanisms: • Large number and variety of participating regulatory elements: thousands of transcription factors (TFs), chromatin, DNA methylation etc. • None of those elements is neither absolutely necessary nor sufficient for the regulatory processes. • T ...
... Characteristic features of gene regulation mechanisms: • Large number and variety of participating regulatory elements: thousands of transcription factors (TFs), chromatin, DNA methylation etc. • None of those elements is neither absolutely necessary nor sufficient for the regulatory processes. • T ...
SHORT COMMUNICATION Genetical Analysis of a
... The second step preceding conjugation is agglutination of cells destined to conjugate, which occurs rapidly after G I arrest when cells of h- and hf mating-types are mixed together (Crandall et aZ., 1977). This agglutination does not occur when ste 1 . 1 is one of the mating partners, whether the ot ...
... The second step preceding conjugation is agglutination of cells destined to conjugate, which occurs rapidly after G I arrest when cells of h- and hf mating-types are mixed together (Crandall et aZ., 1977). This agglutination does not occur when ste 1 . 1 is one of the mating partners, whether the ot ...
Oncogene Activation and Tumor Suppressor
... malignant SCC cell lines. Among the TAM67 transfected SCC cells we observed specific inhibition of both constitutive and inducible AP-1 mediated transcriptional activation, a decrease in the steady state levels of transcripts for certain AP-1 responsive, effector genes, and have demonstrated signifi ...
... malignant SCC cell lines. Among the TAM67 transfected SCC cells we observed specific inhibition of both constitutive and inducible AP-1 mediated transcriptional activation, a decrease in the steady state levels of transcripts for certain AP-1 responsive, effector genes, and have demonstrated signifi ...
Identifying Cells Practice 1. Discuss each answer with your table
... 17. Put the letters in order according to the cell cycle, starting with the interphase cell. ...
... 17. Put the letters in order according to the cell cycle, starting with the interphase cell. ...