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Fermentation
Fermentation

... electron acceptor in its electron transport chain. There are many environments or instances, however, where oxygen is not available to cells or organisms. Consider, for example, the muddy bottom of a bog, or even your muscles when they are rapidly overworked. In both of these instances, oxygen suppl ...
The P5 protein from bacteriophage phi
The P5 protein from bacteriophage phi

... Our analysis of sequence similarity searches and Cystovirus phage genomes suggests that the P5 protein from bacteriophage phi-6, as the only member of peptidase family U40, is homologous to the lytic transglycosylases and has a lysozyme-like fold. This prediction is consistent with the lytic functio ...
Guided reading Ch 9- ENERGY IN A CELL
Guided reading Ch 9- ENERGY IN A CELL

handout nucleic acids and DNA replication
handout nucleic acids and DNA replication

... a) they possess an `anticodon' loop through which they can interact with molecules of mRNA. b) at the opposite end of the molecule they possess an amino acid binding site. The amino acids carried by tRNA molecules eventually form polypeptide chains during protein synthesis. ...
The Citric Acid Cycle
The Citric Acid Cycle

... The citric acid cycle is the final common pathway for the oxidation of fuel molecules: amino acids, fatty acids, & carbohydrates. • Most fuel molecules enter the cycle as acetyl coenzyme A • This cycle is the central metabolic hub of the cell • It is the gateway to aerobic metabolism for any mol ...
1 Determinants of protein-protein interfaces
1 Determinants of protein-protein interfaces

... intermolecular hydrogen bonds and salt bridges being the whole story. However, three of the 54 high-resolution structures studied in [29] have no hydrogen bonds or salt bridges, and another dozen have no salt bridges and five or fewer hydrogen bonds. Thus, we will delve deeper to see what other fact ...
PDF File
PDF File

... The metal ion interaction with the pro-SP oxygen eluded detection for a long time because a single thio substitution at this oxygen was not rescued by soft metal ions such as Mn2+ or Zn2+ (30, 32, and data not shown) and, as shown herein, is only inefficiently rescued by Cd2+ (see below). However, w ...
Part 2
Part 2

... sheet’ which must be superimposed on the gel. When specific anti-phospho-amino acid antibodies or more recently, by this happens, the blue bands and blue spots must motif antibodies that specifically bind to proteins having appear on these sheets. These must then be removed phosphorylation at a part ...
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... The citric acid cycle is the final common pathway for the oxidation of fuel molecules: amino acids, fatty acids, & carbohydrates. • Most fuel molecules enter the cycle as acetyl coenzyme A • This cycle is the central metabolic hub of the cell • It is the gateway to aerobic metabolism for any molecul ...
L -2 Sample preparation Before crystallization (first step
L -2 Sample preparation Before crystallization (first step

... Damage can be during purification, concentration, and storage. Remedies: Include a nuclease inhibitor in the prep or sample to protect the sample from ribonuclease and deoxyribonucleases. Inhibitors: RNasin (from Promega), ribonucleaoside-vanadyl complexes, and DEPC. Inhibitors of deoxyribonucleases ...
Name Date Period 1. What are the end products of aerobic cell
Name Date Period 1. What are the end products of aerobic cell

... The amount of energy absorbed by photosynthetic pigments is equal to the activation energy for photosynthesis. (Total 1 mark) ...
The Organic Chemistry of Enzyme Catalyzed Reactions Revised
The Organic Chemistry of Enzyme Catalyzed Reactions Revised

... Hypothesized that an enzyme is a flexible template that is most complementary to substrates at the transition state rather than at the ground state Therefore, the substrate does not bind most effectively in the ES complex As reaction proceeds, enzyme conforms better to the transition-state structur ...
Essay Prompt #1 - Cloudfront.net
Essay Prompt #1 - Cloudfront.net

... ___Respiratory Assembly: CoQ, cytochromes b, c, a, a3 ___Ring Compounds w/Fe (porphyrin ring) ___Changing Oxidation states as "go down" assembly ___Fe III -> Fe II change ionic state as accept electrons ___Release energy in "packets" - small amounts sufficient to produce ATP (about 7 kcal/mole) ___O ...
Synthesis of Cobalt(III), Iron(III), and Chromium(III) Complexes with
Synthesis of Cobalt(III), Iron(III), and Chromium(III) Complexes with

ATP? - MCC Year 12 Biology
ATP? - MCC Year 12 Biology

... and pH • Outside the optimum conditions, intermolecular bonds are broken leading to a change in the active site and a loss of function • This is called DENATURING the enzyme • Some enzymes can regain their shape, while in others the changes are irreversible. ...
Syllabus for GUTS lecture on Amino Acids
Syllabus for GUTS lecture on Amino Acids

... with the hydrophobic lipid bilayer. Interaction between hydrophobic amino acids is a major force in stabilizing protein structure. Hydrophobic patches may also be found on the surfaces of some cytoplasmic proteins where they often form sites that interact with other proteins. ...
L10v02a_-_glycolysis.stamped_doc
L10v02a_-_glycolysis.stamped_doc

... [00:03:53.52] I'd like to look at a detail of step three in glycolysis. As you know, we invested two molecules of ATP. The first ones produces fructose 6-phosphate. At this point, the cell can still use this molecule for things other than glycolysis or it can be converted back into unphosphorylated ...
Use of Heparin HyperD® M Affinity Resin for Batch Mode Protein
Use of Heparin HyperD® M Affinity Resin for Batch Mode Protein

... are not highly efficient for purification of many important plasma proteins found at low concentration. Affinity chromatography is a preferred method for specific capture and purification of these classes of proteins. Heparin is a negatively-charged mucopolysaccharide consisting of a repeating dimer ...
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... amino acids which are L-isomers. ...
Worked Example 20.1
Worked Example 20.1

... degraded? Note also that the coenzymes NAD+ and FAD are reduced and the reduced versions are considered energy-carrying products of the cycle. Also, CO2 is produced at two different steps in the cycle. Finally, GDP is converted to GTP in Step 5 of the cycle. ...
Transport of Ammonia to the liver
Transport of Ammonia to the liver

... Now when Aspartate combines with citrulline, 1 ATP Molecule is consumed and degraded into AMP + Pyrophosphate (P2O74− ). Pyrophosphate is further degraded to give more energy. How many high energy bonds were used in the cycle ? Answer: 4 Bonds. How many ATPs ? Answer : 3 ATPs Urea Moves from liver t ...
Preview to Mole Activity #2 preview_to_mole_activity_21
Preview to Mole Activity #2 preview_to_mole_activity_21

... This can be interpreted as two molecules of hydrogen and one molecule of oxygen combining to form two water molecules. But how often do we as chemists limit our reactions to one or two molecules? Usually a reaction is done on a billion, trillion scale where an unimaginable number of molecules are in ...
Structure, function and evolution of the XPD family of iron–sulfur
Structure, function and evolution of the XPD family of iron–sulfur

... conserved residues. Several are located on the surface of the archaeal enzyme well away from the ssDNA-binding and active sites, or deep in the hydrophobic core of the Arch domain. Mutation of these residues does not always knock out the helicase activity of the archaeal enzyme [8]. The prediction h ...
Questions and Solutions
Questions and Solutions

... with only vibrations), liquids (fixed volume, particles can slide past one another, and gases (particles move quickly and are separated by lots of space). ...
Stoichiometry
Stoichiometry

... E.g: How many ml of 1 M HCl must be added to 50 ml of 0.5 M HCl to get a solution whose concentration is 0.6 M. no. of moles before mixing = no. of moles after mixing ( Y x1) + (50 x 0.5) = (Y + 50) x 0.6 ...
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Metalloprotein



Metalloprotein is a generic term for a protein that contains a metal ion cofactor. A large number of all proteins are part of this category.
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