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Copper complexes as chemical nucleases
Copper complexes as chemical nucleases

... particularly copper phenanthroline complexes, is primarily sugar directed. Copper complexes usually do not mediate nucleobase oxidation, but are responsible for direct strand scission by hydrogen atom abstraction from the deoxyribose moiety. Sigman and coworkers have shown that bis-(1,10-phenanthrol ...
7. vitamins - Biochemistry Notes
7. vitamins - Biochemistry Notes

... CH3 HO H2C C CH OC NH CH2 CH2 COOH ...
Stable nitrogen isotopic fractionation associated with transamination
Stable nitrogen isotopic fractionation associated with transamination

... The stable nitrogen isotope ratio of glutamic acid is increased by ∼8.0‰ during trophic level changes in ecological food webs. It has been hypothesized that significant isotopic fractionation occurs associated with glutamic acid metabolism during transamination or deamination in heterotrophic animal ...
Two New Ruthenium(II) Complexes with Cyclometalated 2
Two New Ruthenium(II) Complexes with Cyclometalated 2

... underline again the strong labilizing effect of the metal–carbon σ bond on a trans-positioned bond, as was already observed in related ruthenium complexes [1b, 12, 13, 15]. The trans influence of the carbonyl ligand is obviously smaller, although the Ru1–C22 bond is not much shorter than in the comp ...
檔案下載
檔案下載

... malate dehydrogenase ...
2.2.56. amino acid analysis
2.2.56. amino acid analysis

... amino acid analysis of these molecules. The glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes for 1 h in 1 M hydrochloric acid or soak tubes in concentra ...
Enzymes lII: Clinical Applications
Enzymes lII: Clinical Applications

... patterns (Table 8-2) that may be reflected in the relative serum concentrations of the respective enzymes in disease. The diseased tissue can be further identified by determination of the isoenzyme pattern of one of these enzymes (e.g., lactate dehydrogenase, creatine kinase) in the serum, since man ...
Metal Complexes as Antimicrobial Agents
Metal Complexes as Antimicrobial Agents

... Some metals are essential for biological functions and are found in enzymes and cofactors required for various processes. For example, hemoglobin in red blood cells contains an iron porphyrin complex, which is used for oxygen transport and storage in the body. Chlorophyll in green plants, which is r ...
Mader 11 ch 3 Chemistry of Organic Molecules Part 2
Mader 11 ch 3 Chemistry of Organic Molecules Part 2

... Interactions of amino acid side chains with water, covalent bonding between R groups, and other chemical interactions determine the folded three-dimensional shape of a protein. ...


... M. tuberculosis, N. meningitides, MRSA and several other species is a homotetramer in both crystal structure and solution (Figure 3).49 In E.coli, the monomer is 292 amino acids in length and is composed of two domains. Each monomer contains an N-terminal (β/α) 8-barrel (residues 1-224) with the act ...
Purification and Partial Characterization of an Acid
Purification and Partial Characterization of an Acid

... slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sephadex G-100. This suggested that the faster moving component on SDS-polyacrylamide gel el ...
Purification and Partial Characterization of an Acid
Purification and Partial Characterization of an Acid

... slow moving component with a molecular weight of about 155000 and a second protein band of molecular weight 87 100. The molecular weight of the acid phosphatase was estimated to be 93000 by gel filtration on Sephadex G-100. This suggested that the faster moving component on SDS-polyacrylamide gel el ...
Second-Sphere Contributions to Substrate-Analogue
Second-Sphere Contributions to Substrate-Analogue

... been proposed to arise from subtle differences in the second coordination sphere that strongly affect the reduction midpoint potential, Em, of the bound metal ion.1,7,8 By using a combination of spectroscopic and computational techniques in conjunction with site-directed mutagenesis, we were able to ...
Inhibition of the Particle-associated RNA
Inhibition of the Particle-associated RNA

Chapter 25 Organic and Biological Chemistry
Chapter 25 Organic and Biological Chemistry

... © 2015 Pearson Education, Inc. ...
Krebs Cycle - cloudfront.net
Krebs Cycle - cloudfront.net

... matrix of a mitochondrion, where they start the Krebs cycle. The reactions that occur next are shown in Figure 1.1. You can watch an animated version at this link: http://www.youtube.com/watch?v=p-k0biO1DT8&feature=related. Before the Krebs cycle begins, pyruvic acid, which has three carbon atoms, i ...
Side Chain Chemistry Mediates Backbone Fragmentation in
Side Chain Chemistry Mediates Backbone Fragmentation in

... via the 18C6 portion of the molecule. The preferred target is lysine; however, under appropriately gentle electrospray conditions, 2 will complex with virtually any protonated peptide. The iodonaphthyl portion of 2 is designed to yield a naphthyl radical following photodissociation of the carbon-iod ...
Biochemistry The Citric Acid Cycle Chapter 17:
Biochemistry The Citric Acid Cycle Chapter 17:

... to enol intermediate ...
Krebs cycle
Krebs cycle

... Very similar to PDC but has no intrinsic protein kinases & phosphatases. Otherwise has ~ the same regulation ...
Document
Document

... during and after exercise. This indicated that the glucose-fed rats burned sugar over body fat as an energy source.3 By contrast, the whole-milk protein and whey meals preserved lipid oxidation and increased protein oxidation, indicating that fat burning was maintained and that protein was also used ...
The KIebsieIIa pneumoniae cytochrome bd
The KIebsieIIa pneumoniae cytochrome bd

... severely impaired in the presence of low concentrations of oxygen compared with the wild-type bacterium. Only the wild-type organism was capable of microaerobic nitrogenase activity supported by fermentation products. It is proposed that formate dehydrogenase-0may be involved in supplying electrons ...
Discussion: Why is the addition of Super-Conductive
Discussion: Why is the addition of Super-Conductive

... Why is the addition of Super-Conductive Ionic Minerals™ in your diet so important? Soil depletion, chemical pollution, and other environmental factors have contributed to an acute lack of minerals in almost everyone's diet. This was first noted at the beginning of the last century and the mineral de ...
Review Article Hydroxyl radical generation theory: a possible
Review Article Hydroxyl radical generation theory: a possible

... unchecked, ethanol molecules is not the only target for hydroxyl radicals. It all makes the peroxidatic activity relatively slow. Role of NADPH Hydrogen peroxide provides both oxidative and reductive potential during catalysis. Because of this the precise role of cofactor (NADPH) in enzyme activity ...
Glycolic Acid Labeling During Photosynthesis
Glycolic Acid Labeling During Photosynthesis

... acid from CO, one might expect a significantly higher ratio of tritium to :'C in glycolic acid than in other intermediates of the photosynthetic carbol redutction cycle, after a short period of photosynthesis with both tracers. Also, one would expect the T/14C ratio of glycolic acid to drop with tim ...
Porphyrins (Structure of Porphyrins)
Porphyrins (Structure of Porphyrins)

... Porphyrins are cyclic compounds that readily bind metal ions, usually Fe2+, Fe3+. The most prevalent metallopophyrin in human is heme, which consists of one Fe2+ coordinated in the center of tetrapyrrol ring protoporphyrin lX through methenyl bridges. Heme is the prothetic group for hemoglopin, myog ...
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Metalloprotein



Metalloprotein is a generic term for a protein that contains a metal ion cofactor. A large number of all proteins are part of this category.
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