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Gene Patents in India: Gauging Policy by an Analysis of the
Gene Patents in India: Gauging Policy by an Analysis of the

... discloses the full length nucleotide sequence of the JEV strain, the infectious JEV cDNA clones (which can be obtained from the genomic sequence) and the use as a vector after introducing the cDNA in a BAC vector. Here, the creation of the cDNA is a part of the larger process of creating a vector to ...
uniprotkb-goa_aug2011
uniprotkb-goa_aug2011

... • Determines which GO terms are more often associated with a specified list of genes/proteins compared with a control list or rest of genome • Many tools available to do this analysis • User must decide which is best for their analysis ...
PowerPoint 簡報
PowerPoint 簡報

Extraordinary Sequence Divergence at Tsga8, an X
Extraordinary Sequence Divergence at Tsga8, an X

... for sequencing with Qiagen QIAquick spin columns (Qiagen, Valencia, CA) or using the University of Arizona Genetics Core (UAGC) cleaning service. Cleaned PCR products were sequenced by UAGC using an ABI 3731 automated sequencer and big-dye terminator chemistry. Chromatograms were edited and initiall ...
Al - Iraqia university/ college of medicine
Al - Iraqia university/ college of medicine

... The base can have 2 rings (adenine or guanine) or one ring (thymine or cytosine). In RNA, the base uracil (U) replaces base thymine. These structures are called bases because their presence raises the pH of a solution. Polynucleotide Structure: Nucleotides link to make a polynucleotide, called a str ...
Lack of homology between two haloacetate dehalogenase genes
Lack of homology between two haloacetate dehalogenase genes

... Two genes encoding haloacetate dehalogenases,H-1 and H-2, are closely linked on a plasmid from Moraxellu sp. strain B. H-1 predominantly acts on fluoroacetate, but H-2 does not. To elucidate the molecular relationship between the two enzymes, we compared their structuralgenes. Two restriction fragme ...
- Wiley Online Library
- Wiley Online Library

... for chemical modifications. Histones provide the primary packing structure for chromosomal DNA in eukaryotes with each histone wrapped in ∼146 bp of DNA to form the nucleosome, and are structured from two copies each of four different subunits: H2A, H2B, H3, and H4, whose residues are subjected to a ...
Chapter 4 Background DNA Structure and Analysis
Chapter 4 Background DNA Structure and Analysis

... • Main focus on genes and their function ...
20.6 NnV mx
20.6 NnV mx

... genes are occasionally assembled into regulatory units called operons2. On page 851 of this issue, Blumenthal et al.3 now report the first whole-genome characterization of such operons in a multicellular organism, and raise intriguing questions as to how (and why) they have evolved. Operons were fir ...
genetic code
genetic code

... genetic code: means for converting DNA sequence into protein sequence the original question has always been how to convert 4 nucleotide bases into 20 types of amino acids in the 1940's Beadle and Tatum begain studying a bread mold Neurospora and isolated mutants (ie. strains of yeast with damaged ge ...
Chapter 5: Nucleic Acids, etc. Nucleotides and Derivatives Nucleic
Chapter 5: Nucleic Acids, etc. Nucleotides and Derivatives Nucleic

... (a) AUCGp – assumes phosphate between nucleotides (b) ApUpCpGp – all phosphates indicated with 'p' Proper Name – adenyl-3',5'-uridyl-3',5'-cytidyl-3',5'-guanyl-3'phosphate (only used for small or uncommon oligonucleotides) ...
Chapter 19 (Eukaryotic Genome)
Chapter 19 (Eukaryotic Genome)

... – The looped domains themselves coil and fold forming the characteristic metaphase chromosome ...
emboj7601881-sup
emboj7601881-sup

... viral proteins involved in virus genome replication are expressed stably from plasmids ...
qPCR Hand Calculations - University of Puget Sound
qPCR Hand Calculations - University of Puget Sound

... NOTE: These calculations apply when only one reference gene is being used. If you are using more than one reference gene (which is recommended) please use the document titled “qPCR Hand Calculations when using Multiple Reference Targets” ...
Detection of Viral, Bacterial and Human Genomic DNA
Detection of Viral, Bacterial and Human Genomic DNA

Piwi-interacting RNAs and the role of RNA interference
Piwi-interacting RNAs and the role of RNA interference

... RNAi since the RNAi drugs can be delivered directly to the diseased tissue through injections to the eye. These RNA strands are not packaged or protected in any way, so they are referred to as “naked” RNAi drugs. In this example they can easily reach their intended target intact since traveling thro ...
BCB 444/544
BCB 444/544

Exam 2
Exam 2

... Fossil D Volcanic ash – no fossils found Fossils A and C Fossil B Fossil A Question 15 From the data it can be concluded that A. fossil A is less than 5 million years old. B. fossil D is the youngest fossil present. C. fossils A and C are closely related. D. fossil B is extinct. Question 16 Fossils ...
Quant-iT™ Assay Kits for microplate
Quant-iT™ Assay Kits for microplate

... linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of ...
Lecture
Lecture

... Function: Storage of amino acids Examples: Casein, the protein of milk, is the major source of amino acids for baby mammals. Plants have storage proteins in their seeds. Ovalbumin is the protein of egg white, used as an amino acid source for the developing embryo. ...
Mammalian Two-Hybrid Assay Kit
Mammalian Two-Hybrid Assay Kit

Leukaemia Section t(3;6)(q27;p21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(3;6)(q27;p21) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... 706 amino acids; composed of a NH2-term BTB/POZ domain (amino acids 1-130 (32-99 according to SwissProt)) which mediates homodimerization and proteinprotein interactions with other corepressors (including HDAC1 and NCOR2/SMRT) to constitute a large repressing complex, another transcription repressio ...
Slide 1
Slide 1

... Gene map locus 17q21.31-q22, 7q22.1 ...
Case Study Powerpoints - Westford Academy Ap Bio
Case Study Powerpoints - Westford Academy Ap Bio

... second complementary DNA strand to be incorporated into host’s DNA. When a person is infection with HIV host cells retain provirus in their own genome. Immune system cannot detect HIV in host cell and provirus cannot be removed from body as foreign. At any time provirus can produce more viral mRNA a ...
Expression and purification of four different rhizobial acyl carrier
Expression and purification of four different rhizobial acyl carrier

... and the general tertiary fold of the NodF protein (Ghose et al., 1996) demonstrates that the overall structures of ACPs are surprisingly well conserved. In spite of the general structural similarity between NodF and AcpP of E. coli, AcpP cannot substitute for NodF in vivo in the synthesis of polyuns ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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