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Metabolism of amino acids, porphyrins
Metabolism of amino acids, porphyrins

... •The others are classed as "essential" amino acids and must be obtained in the diet ...
Genetics Problems
Genetics Problems

... their offspring that would need glasses resulting from the parental cross of a heterozygous farsighted man and a woman with normal eyesight. 3. Short hair (S) is dominant over long hair (s) in rabbits. Determine the probable offspring of the mating of a male homozygous short-haired rabbit and a fema ...
Frequently Asked Questions about Red/ET Cloning
Frequently Asked Questions about Red/ET Cloning

... lower concentration. Normally, it shows a bit lower efficiency than a PCRgenerated fragment. A sometimes observed, higher efficiency after precipitation, maybe due to a higher DNA concentration that has been applied, to prevent from unsatisfying yield. You once marketed several kits, including kits ...
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... sample. Potassium ions interact with the SDS making the detergent insoluble. The SDS will easily precipitate and can be separated by centrifugation. In doing so the insoluble SDS traps the larger genomic DNA and removes it from the supernatant. This leaves the plasmid DNA and RNA in solution. The RN ...
Solid Tumour Section Soft tissue tumors: Rhabdomyosarcoma Atlas of Genetics and Cytogenetics
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... (20%); polymorphism studies shows that E-RMS is associated with the loss of heterozygosity at 11p13. A-RMS is characterized by two pathognomonic translocations: t(2;13)(q35;q14) and t(1;13)(p36;q14), found in 80 and 15% of the cases respectively, leading to the formation of gene fusions, namely PAX3 ...
another handout on atherosclerosis
another handout on atherosclerosis

... V) What if such a combination of mutations produced ten times worse symptoms, as compared with either mutation alone. Can you invent a hypothesis that would predict that? VI) Please consider whether genetic difference that favor development of atherosclerosis (Cause worse symptoms? Earlier onset? Mo ...
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Grade 12 Biology: Final Exam

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supplemental figure legend and Materials and Methods

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... Aims of genetics course  Understand the definition and branches of genetics.  Understand the key features of chromosome and gene.  Describe the key steps in the cell cycle.  Describe the relationship between mitosis and meiosis in both ...
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... c) Write the protein fragment that the mRNA strand in (b) above would code for. d) If the 4th nucleotide from the left in the mRNA strand above were changed from U to C, what mutation is this and what would the resulting mRNA look like? ...
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... Another useful concept is the statement that “chance has no memory.” This concept helps us know that each child has the same chances. So, if a couple has four children, each child has a 25% chance of having attached earlobes. This may not be significant if we are considering earlobes. It does become ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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