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Yeast - iGEM 2007
Yeast - iGEM 2007

... Yeast genome: fully sequenced and easy to manipulate. Basic mechanisms of yeast cell biology (such as DNA replication, recombination, cell division and metabolism) are highly similar to that of higher organisms (including humans). ...
3 Meiosis
3 Meiosis

... happen, he realized that genes must be located on the chromosomes. One; there is only one possible allele from each parent. The offspring will all have the same combination. XY Students should circle the XX combinations. a disorder whose genes are carried on the X chromosome first row, left to right ...
Genome analysis of Desulfotomaculum gibsoniae strain
Genome analysis of Desulfotomaculum gibsoniae strain

... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
Molecular markers in Brassica Rapa
Molecular markers in Brassica Rapa

... of BrCRTISO2 in a normal yellow-colored (YE) cultivar, but full-length BrCRTISO1 transcripts were not detected in the OC cultivar. Genomic sequence analysis revealed that BrCRTISO1 of the OC cultivar had many sequence variations, including single nucleotide polymorphisms (SNPs) and insertions and de ...
Managing people in sport organisations: A strategic
Managing people in sport organisations: A strategic

... Mechanisms for generating variant surface glycoprotein diversity in trypanosomes: Expressing new VSG. VSG sequences are in shades of red, others are purple. Silent VSG genes are dark red; expressed VSG genes are bright red, and VSG pseudogenes are pink. The large dots at the end of the chromosome re ...
Nucleic Acids
Nucleic Acids

... the antiparallel strand must be thymine. Similarly, if the purine in one strand is guanine, its complement in the antiparallel strand must be cytosine. A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair o ...
Variable clinical manifestation of a novel missense mutation in the
Variable clinical manifestation of a novel missense mutation in the

Translation of Cyclin mRNA Is Necessary for Extracts of Activated
Translation of Cyclin mRNA Is Necessary for Extracts of Activated

... al., 1979; Kishimoto et al., 1982; Weintraub et al., 1982; Gerhart et al., 1984; Meijer and Guerrier, 1984). MPF can also drive GP-arrested cells into mitosis (Miake-Lye et al., 1983; Newport and Kirschner, 1984; Kirschner et al., 1985). Thus, MPF is an almost universal concomitant of M phase, and i ...
Imprinted SNRPN within chromosome territories
Imprinted SNRPN within chromosome territories

... sets: (5′-AGGAAAAGGAACAGGGTAAGGGAAA-3′, 5′-GCAGACGCAGCAGAGGTGACAG-3′, 5′-ACGCAACACAGACCCCCAGG-3′, 5′-GGAAGGGCGGTGGTGACTGG-3′) corresponding to approximately 3 kb sequences on the upstream of exon alpha of the SNRPN gene. PCR products were labeled by nick translation using digoxigenin-11-dUTP. The RN ...
Ch. 9 Patterns of Inheritance (Lecture Notes)
Ch. 9 Patterns of Inheritance (Lecture Notes)

... Mendel’s Law of Segregation. Each organism contains two factors (alleles) for each trait (genes), and the factors segregate during the formation of gametes so that each gamete contains only one factor from each pair of factors. When fertilization occurs, the new organism will have two factors for ea ...
Gene Section MIR7-1 (microRNA 7-1) Atlas of Genetics and Cytogenetics
Gene Section MIR7-1 (microRNA 7-1) Atlas of Genetics and Cytogenetics

Gene Expression in Adult Metafemales of Drosophila
Gene Expression in Adult Metafemales of Drosophila

... The expression of selected X-linked and autosomal genes was examined in metafemales ( 3 X 2 A ) compared to diploid sisters. Three enzyme activities (glucose-6-phosphatedehydrogenase, 6-phosphogluconate dehydrogenase, p-hydroxyacid dehydrogenase) encoded by X-linked genes are not significantly diffe ...
Monohybrid cross
Monohybrid cross

... by the U.S. Department of Labor’s Employment and Training Administration (CB-15-162-06-60). NCC is an equal opportunity employer and does not discriminate on the following basis: against any individual in the United States, on the basis of race, color, religion, sex, national origin, age disability, ...
Lecture 4 - University of California, Santa Cruz
Lecture 4 - University of California, Santa Cruz

... world. In parts of Africa frequencies of 20% to 40% are often found for the HbS allele. It was found however that in areas in which there was a high HbS allelic frequency, that there was also a corresponding high frequency of mosquitoes infected with the protozoan parasite, plasmodium. This parasite ...
Transduction of mammalian cells using lentiviral vectors
Transduction of mammalian cells using lentiviral vectors

... Known oncogenes or shRNAs that will silence known tumour suppressor genes may be inserted into primary cells and cell lines. There is therefore a theoretical possibility that they could render the cells neoplastic. If during an unlikely event these cells were inoculated into the human organism, then ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma

... A unique feature of APOA5 compared with its evolutionarily related apolipoprotein paralogues is its multiple transcripts of ⬇1.3 and 1.9 kb, as determined by Northern blot analysis.8 Alternative transcripts have not been described for other members within this gene cluster. Examination of expressed ...
Advances in the diagnosis of infection
Advances in the diagnosis of infection

... acid to increase the signal generated from a hybridised probe molecule. Commonly used technologies include branched DNA (bDNA) and hybrid capture assays Probe amplification The end-product of the reaction is an amplified version of the original components used to detect the target and include the li ...
περισσότερες πληροφορίες
περισσότερες πληροφορίες

... Lilly and company today announced that it has begun limited testing in healthy human volunteers of biosynthetic human insulin produced by recombinant DNA technology (followed careful animal studies and extensive laboratory testing). The company has started construction of the world’s first manufactu ...
The most abundant protein in bovine herpes 1 virions is
The most abundant protein in bovine herpes 1 virions is

Chapter 12 Reproduction and Meiosis
Chapter 12 Reproduction and Meiosis

... During the somatic cell division process, each homologous chromosome moves independently, and two sister chromatids of the single chromosome are divided into two cells during the fission process. In meiosis, on the other hand, homologous chromosomes form pairs (synapsis). This pairing also occurs be ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma

... A unique feature of APOA5 compared with its evolutionarily related apolipoprotein paralogues is its multiple transcripts of ⬇1.3 and 1.9 kb, as determined by Northern blot analysis.8 Alternative transcripts have not been described for other members within this gene cluster. Examination of expressed ...
Gene Section FABP7 (fatty acid binding protein 7, brain)
Gene Section FABP7 (fatty acid binding protein 7, brain)

... Crystal structure of FABP7 bound to DHA. The structure of FABP7 is similar to that of other FABPs and consists of two Nterminal α-helices attached to a β-barrel motif (left). Three amino acids are predicted to be important for fatty acid binding: F104 (fuchsia), arginine 126 (red) and Y128 (teal) ba ...
Nucleic Acid Synthesis in the Neoplastic Cell
Nucleic Acid Synthesis in the Neoplastic Cell

... Other pyrinfidine and purine analogs may be incorporated into D N A and RNA. Iododeoxyuridine may replace thymine in the D N A of human leukemic leukocytes following the in vivo administration of the analog. Iododeoxyuridine is also incorporated into D N A of tissue culture cells (73). Fluorouracil, ...
Document
Document

... What do these codons have to do with proteins? • Each codon represents an amino acid that will eventually form a protein that is used within a cell. • Proteins are made up of hundreds of amino acids in a specific sequence. • When they get “out of order’ a mutation occurs. ...
Population genomics of human gene expression
Population genomics of human gene expression

... We estimated the median and variance of each of the 47,294 To maximize the power to detect genetic effects, it is important to probe types for each population, and analyzed the distribution of establish that expression measurements are robust to experimental variance and median values of normalized ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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