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Predicted function of the vaccinia virus G5R protein
Predicted function of the vaccinia virus G5R protein

... each protein associated with the intracellular mature virion using mass spectrometry were unable to detect G5R within the intracellular mature virion (Yoder et al., 2006; Chung et al., 2006). When we employed a hidden Markov model (HMM) comparison search tool (HHsearch) to screen for the functions o ...
What is similarity and homology? What is a good match? How does
What is similarity and homology? What is a good match? How does

... Repeats and low complexity regions constitute more than one third of the human genome. Highly locally biased composition occurs in regions of many proteins and in DNA. E.g. structural proteins in hair. Low complexity regions may give rise to high alignment scores – but are usually biologically unint ...
Protein splicing elements: inteins and exteins
Protein splicing elements: inteins and exteins

... The protein sequences that flank the intein, and that are ligated to form the mature product, are defined as the exteins. Exteins are analogous to RNA exons. This nomenclature should replace that used in previous publications, where exteins were called 'external protein sequences' or 'EPSs'. We furt ...
Site specific insertion of a type I rDNA dement into a unique
Site specific insertion of a type I rDNA dement into a unique

... panel); or CR.H6/11, a Hindlll fragment from a recombinant phage carrying a segment of Drosophila DNA carrying the 'empty' site (left panel). The CR.H6/11 probe is in M13mp8. shown). This experiment indicated that the Hindlll fragment 8a/5 was present as a single copy within the D. melanogaster geno ...
Diapositive 1 - LBGI Bioinformatique et Génomique Intégratives
Diapositive 1 - LBGI Bioinformatique et Génomique Intégratives

... ARP11 are involved in microtubule based vesicle trafficking, in nuclear functions (transcriptional activation, tumor suppression…), ARP4-ARP9 are components of many chromatin modulation complexes (SWI2/SNF2, SWR1, HAT). Conventional actins and ARPs together define a large family of homologous protei ...
Other crosses - No Brain Too Small
Other crosses - No Brain Too Small

... The development of coat colour patterns in cats is very complex. It involves at least 10 genes, each of which has a different effect on the phenotype. Effects include different colours, spots, different intensities, stripes, colour in different parts of the body and texture. (a) Explain why the 10 g ...
Sex-linked traits
Sex-linked traits

... Law of Independent AssortmentSeparate genes for separate traits are passed independently of one another from parents to offspring. These allele pairs are then randomly united at fertilization. ...
iGenetics: A Molecular Approach, 3e (Russell/Bose)
iGenetics: A Molecular Approach, 3e (Russell/Bose)

... 43) The nucleus separates the chromosomes from the rest of the cell. How is the information in the DNA communicated to the cell? Answer: The nuclear membrane has pores and is permeable to certain molecules. This allows selected materials, like RNA and proteins, to move in and out of the nucleus. Ski ...
Chapter 15
Chapter 15

... 1. How was it determined that chromosomes carry genes? 2. Morgan’s next cross showed that linked genes are inherited together. 3. What if the genes were unlinked…meaning independent assortment? 4. How often will recombination occur…frequency?? 5. How can a genetic map be created from recombination f ...
EXTENDED CONCEPT OF KNOWLEDGE FOR EVOLUTIONARY
EXTENDED CONCEPT OF KNOWLEDGE FOR EVOLUTIONARY

... makes it a gene, and what or who could be that 'somebody' (and in what sense). There is a real danger to take these metaphors too literally, and as a consequence to load them with spiritualist metaphysics or religious mysticism. I think this danger is one apparent reason why this kind of 'contentiou ...
Biology Chapter 10 Meiosis Notes 3-27
Biology Chapter 10 Meiosis Notes 3-27

... Crossing Over  Takes places during Prophase I of Meiosis  Homologous chromosomes in tetrad o Chromosomes exchange corresponding segments of their chromatids (sections that carry the same genes) – 1-3 locations per chromosome pair ...
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Slide 1

... • Bootstrap Aggregating (Bagged) Logic Regression is a new technique that may be useful in analyzing SNP associations. • Bagged Logic Regression identified “Worst-Least” CFS SNP genes consistent with exhaustive search by Goertzel, et al (2006). • “Interesting” SNPs did not show statistically signifi ...
MALE STERILITY - public.iastate.edu
MALE STERILITY - public.iastate.edu

... 3. Most revertants show that T-urf13 is deleted from mitochondrial genome by recombination, resulting in a simultaneous loss of cms and disease susceptibility; some revertants due to mutations (frameshift mutation or premature stop codon) in T-urf13 that make non-functional URF13 protein. These data ...
Molecular identification of rapidly growing mycobacteria
Molecular identification of rapidly growing mycobacteria

... (Daley and Griffith, 2002). RGM identification at species level is necessary because it provides the first indication regarding the mycobacteria antibiotic susceptibility. Identification of these organisms by biochemical methods is not always a straightforward procedure (Brown-Elliott and Wallace, 2 ...
Lecture 9 Fatty Acid Synthesis
Lecture 9 Fatty Acid Synthesis

... Malonyl-CoA by Acetyl-CoA Carboxylase (ACC) Biotin: water soluble vitamin – functions as a CO2 carrier for several important reactions including:  Acetyl-CoA carboxylase  Pyruvate carboxylase  Propionyl CoA carboxylase ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII

... events because the telomere reFigure 1 Probes H49 and JL8 define a pair of size-polymorphic homologous chromosomes. (A) peat arrays of the T. cruzi chroSeparation of T. cruzi chromosomal bands by PFGE and staining with ethidium bromide. The mosomes are short, ranging in arabic and roman numerals ind ...
Life: The Science of Biology, 8e
Life: The Science of Biology, 8e

... needed for conjugation; F factor can be transferred during conjugation. ...
MIDAS2_19
MIDAS2_19

... One-class SAM: Identify genes whose mean expression across experiments are different from a user-specified mean. • Assign a score (d) to each gene based on its change in expression relative to the standard deviation of repeated measurements for the gene • Genes with scores > a threshold (Δ) are deem ...
Title: Gene Interactions in Corn. Introduction. The phenotype of an
Title: Gene Interactions in Corn. Introduction. The phenotype of an

... genotype of an organism, and an understanding of how the various genes and alleles interact, we can predict the phenotype of the progeny of a particular cross. For example, given that flower colour in pea plants is controlled by a single gene, and that the allele that gives rise to purple flowers is ...
Amino Acids and Proteins: →Protein Functions: enzymes, transport
Amino Acids and Proteins: →Protein Functions: enzymes, transport

... →Can have cis or trans form, but trans is the most stable conformation, so find trans peptide bonds in proteins. Exception: Pro-because the NH group is attached in a ring structure, cis and trans have about equal energy. Therefore, whether it is cis or trans at the Pro position depends on the overa ...
Aromatic Amino Acid Hydroxylase Genes and
Aromatic Amino Acid Hydroxylase Genes and

Microsoft Word (Chapter 3) - DORAS
Microsoft Word (Chapter 3) - DORAS

... Analysis of the results indicated that all siderophores tested were utilised by S. meliloti. Some background growth was evident on the plates culturing S. meliloti 2011 which was due to the production and utilisation of the endogenous siderophore rhizobactin 1021. The indicator strain S. meliloti 20 ...
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Slide 1

... Don’t forget to go: …coding DNA …template DNA …mRNA …A A ...
Protein-only inheritance in yeast: something to get
Protein-only inheritance in yeast: something to get

... In another group of experiments, the proteinremodelling factor Hsp104 was isolated as an extra-copy modifier of [PSI1] in a genetic screen10. Either the deletion or the overexpression of Hsp104 heritably eliminates [PSI1] from yeast strains, and this change in phenotype is accompanied by a change in ...
Genetics - Elizabeth Rose Greenman
Genetics - Elizabeth Rose Greenman

... • He crossed plants with two different traits, for example purple flowers with white flowers. • He started his experiments with purebred plants. • Purebred plants ALWAYS produce offspring with the same trait as the parent. For example, if the parent is tall, all offspring will be tall. If the parent ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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