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3 Genetics - Kerboodle
3 Genetics - Kerboodle

Expressed Sequence Tags
Expressed Sequence Tags

... randomly selected from genome library and can be used to identify and map the whole genome of any particular species. ESTs are usually 200 to 500 nucleotides long and are generated by sequencing the ends of DNA. ESTs can be obtained without much expenditure and are quite fast in genomic analysis. Th ...
Pathology
Pathology

... 1) Know the major types of mutations and be able to give an example of each. Point Mutation (missense, nonsense) – replacement of one base pair with another creating a codon for a different amino acid (missense) or a stop codon (nonsense). Ex: Sickle cell anemia. Frameshift (deletion or insertion) – ...
Life Science NJ ASK Review
Life Science NJ ASK Review

... • Nucleus usually pushed to • Nucleus • Nuclear Membrane the side • Cell Membrane • Usually rectangular(ish) • Cytoplasm • Golgi Apparatus ...
Vocabulary handout
Vocabulary handout

... In order to make sense of mitosis and meiosis one must have some basic chromosome terminology. Duplicated Vs Unduplicated Chromosomes Chromosomes either have one or two molecules of DNA plus associated proteins. A chromosome with one molecule of DNA is called an unduplicated chromosome because it on ...
Mitosis and Meiosis Power Point
Mitosis and Meiosis Power Point

...  Sex chromosome ...
DNA Replication
DNA Replication

... • DNA replication is carried out by a complex system of enzymes. • Some regions of DNA do not code for proteins but have other important functions. Applications and Skills • Use of nucleotides containing dideoxyribonucleic acid to stop DNA replication in preparation of samples for base sequencing. • ...
BI-Lec 3
BI-Lec 3

... compiled from a DNA or cDNA Draft sequences library. Usually large collection of contigs and are in the process of being ordered and catalogued. Genome ...
regulation of cell cycle
regulation of cell cycle

... The 5' cap is a modified guanine nucleotide added to the "front" (5' end) of the pre-mRNA using a 5',5-Triphosphate linkage. This modification is critical for recognition and proper attachment of mRNA to the ribosome, as well as protection from 5' exonucleases. It may also be important for other ess ...
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Document

... bacterium Agrobacterium tumefacians Used because it evolved to be recognized in most plants ...
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... than are at lower temperatures, indicating that the fungus is under heat stress. 2. More putative virulence genes (ex. those coding for the proteins responsive to oxidative stress and host immune system and for toxin production) are highly expressed at 37˚C, although there is no contact with host ce ...
EOC Review Packet #2
EOC Review Packet #2

... 5. What are the 4 bases of RNA? Which ones pair together? 5. A-U, C-G 6. What would be the mRNA sequence of the following DNA sequence? ...
Name - Valhalla High School
Name - Valhalla High School

... 3. Use the diagram to label the generations: P, F1, F2, pure, hybrid, and make notes of Mendel’s observations. ...
Study Guide: Meiosis and Genetics
Study Guide: Meiosis and Genetics

... 3.3.1 What are the 3 building blocks of the DNA molecule? 3.3.2 What are the complete names for the 4 bases found in DNA? How do these pair up? 3.3.3 What type of chemical bonds hold together the nucleotides? 3.3.4 What type of bonds hold together the complementary strands of DNA? How do these compa ...
m02-biological_sequences
m02-biological_sequences

... This lecture introduces biological sequences (e.g. genomes, genes, transcripts, and proteins) as an important biological data type, as well as methods and tools for representing, storing, and searching sequence data. Biopolymers Nucleic acids and polypeptides are biological polymers comprised of spe ...
Field Guide to Methylation Methods
Field Guide to Methylation Methods

... groups to nucleic acid bases. In addition to 5-methylcytosine, other common modifications are 7-methylguanosine (5’-cap for RNA), and 6-methyladenosine (common RNA modification.) CpG island Defined as regions > 500 bp, > 55% GC and expected/observed CpG ratio of > 0.65. 40% of gene promoters contain ...
Macromolecules of the Human Body
Macromolecules of the Human Body

... Pancreatic nuclease enzymes digest nucleic acids (DNA and RNA) to nucleotides in the duodenum. Membrane-bound nucleotidase enzymes in the epithelial cells of the ileum digest the nucleotides to sugar, base and phosphate, which are absorbed. ...
DNA and Proteins
DNA and Proteins

... a combination of three nitrogenous bases, called a codon, to code for one of 20 common amino acids. There are 64 codons possible. • Some codons code for instructions such as start (AUG) and stop (UAA). • The same amino acid can have more than one codon. ...
The Genetic Basis of Development
The Genetic Basis of Development

... an enucleated egg (or egg that has had nucleus removed) of the same species can support normal development (reproductive cloning)  however, the older the donor nucleus the lower the % of normal development so something in the animal nucleus does change as animal cells differentiate ...
Personalized Medicine Background and Challenges Geoffrey S
Personalized Medicine Background and Challenges Geoffrey S

... Genetic counselors will play an increasingly important role in patient management as genetic information becomes incorporated into everyday clinical practice Referrals to genetic counselors are encouraged; if no counselors are available at one’s institution, local counselors can be found through the ...
DNA ANALYSIS: Public vs private access to the human genome
DNA ANALYSIS: Public vs private access to the human genome

... Part 1. b. Locate the following information about your sequence and circle or list them i) What does the cDNA encode? What is the function of the protein product? (Note: to fully answer this, you may need to do further searches) ii) Genus and species name of the organism the cDNA is from. iii) The v ...
University students` conceptions about the concept of gene - Hal-SHS
University students` conceptions about the concept of gene - Hal-SHS

... But, it is also found that, hybrid models consisting of features from several of the historical models. 26. 8% of students knew the chemical nature of gene (DNA) and defined the gene by its relationship to a phenotype regardless of the specific molecular sequence and the whole developmental mechanis ...
Antimicrobial Drugs
Antimicrobial Drugs

... permeability by binding to sterols in fungal membranes, more side effects since membranes similar in all cells) Protein synthesis ...
Types of RNA
Types of RNA

... 1. ______________________________ - Compounds made of sugar molecules (saccharides) 2. ______________________________ - Stores energy for long term use. Includes Fats, phospholipids , waxes, and steroids 3. ______________________________ - Long polymers of which amino acids are connected together by ...
transcription and rna
transcription and rna

... Degeneracy of the genetic code and the wobble hypothesis Degenerate genetic code: some amino acids are specified by more than one codon Wobble hypothesis Codon-anticodon pairing precise for first two nucleotides of codon Base-pairing rules at third codon position (3’-end) is less constrained The gen ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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