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BIOL 504: Molecular Evolution
BIOL 504: Molecular Evolution

... Neofunctionalization: one copy acquires a beneficial mutation that results in a new function Ancestral polymorphisms can also facilitate neofunctionalization Example: insecticide resistance in mosquito. Acetylcholinesterase enzyme plays essential role in central nervous system. Mutant allele at dupl ...
Biol
Biol

Self-incompatibility
Self-incompatibility

... •Self-crossing is much more common in plants than animals. •The reason many plants can inbreed may be due the relative importance of the gametophyte generation. •The superior performance of an F1 from inbred parents is call Hybrid Vigour. It is very important in crop production. Selfing F1 ...
pptx formatted for Benson Hill Biosystems
pptx formatted for Benson Hill Biosystems

... • Previous mutated versions have shown promise in field trials of multiple crops • Iterative mutation has resulted in BH33, which has improved characteristics relative to enzymes previously expressed in plants • Maize field testing in 2014 showed up to 24% increase in ear weight relative to control ...
xianxu
xianxu

... Virtual Gene Algorithm • Gene to gene correlations are generally ignored in feature selection algorithms. In this work, we examine using instead of ignoring such correlations for the purpose of gene selection. • Motivating examples are shown in the next two pages, from both synthetic and real ...
Transcription & Translation
Transcription & Translation

... • Translation of mRNA information into protein starts at the initiation codon (5’AUG3’); proceeds codon by codon until the end of gene, as identified by a stop, or non-sense, codon. • Coding region of mRNA can be polygenic (multiple genes = multiple starts / stops). Coding Region ...
What is a DNA?
What is a DNA?

... • Add an equal volume of isopropanol. • DNA will be precipitated by gentle swirling & observed as a white thread like strand. • Using a sterile spatula or loop transfer the DNA strand into a sterile micro centrifuge tube containing 1 mL of 75% ethanol. • Wash by inversion to remove any remaining sal ...
Quantitative Traits
Quantitative Traits

... Quantitative traits are determined by many genes spread across numerous chromosomes. The alleles of quantitative genes are additive. So it is possible to have many combinations of the additive traits. What results is a continuous range of variation. Traits which are controlled by genes that fall wit ...
Structure and function of DNA
Structure and function of DNA

... Iso _________  arg  tyr  ala  leu ...
Biochemistry Powerpoint
Biochemistry Powerpoint

... Enzymes in the Human Body(2700 enzymes) 1. Amylase in saliva speeds the breakdown of starch (catabolic) 2. Lipase from the pancreas speeds the breakdown of lipids in the small intestine (catabolic) 3. Polymerase is used in building DNA from monomer nucleotides. (anabolic) 4. GTPases work in the cel ...
Isolation and Sequencing of Actin1, Actin2 and Tubulin1 Genes
Isolation and Sequencing of Actin1, Actin2 and Tubulin1 Genes

... physiological characteristics, no efficient treatments against diseases caused by these microorganisms are presently available [3]. In order to develop such treatments appeared essential to dissect the molecular mechanisms of the interaction between Phytophthora species and host plants. Actin and tu ...
Large Scale Protein Association Prediction
Large Scale Protein Association Prediction

... • There are other aspects of function: functional associations, e.g. the target of a protein kinase or a transcriptional regulator, I.e. to understand the cell we need to know the interactions of the genes Thus: predicting associations ...
Table of Genetic Disorders Disease Gene/Defect Inheritance
Table of Genetic Disorders Disease Gene/Defect Inheritance

Download: Genes, Genomics, and Chromosomes
Download: Genes, Genomics, and Chromosomes

... length. Satellite DNA consists of 14-500 bp sequence units that tandemly repeat over 20-100 kb lengths of genomic DNA. Minisatellite DNA consists of 15-100 bp sequence units that tandemly repeat over 1-5 kb stretches of DNA. Microsatellite DNA consists of 113 bp units that can repeat up to 150 times ...
The glpP and glpF genes of the glycerol regulon in
The glpP and glpF genes of the glycerol regulon in

... DNA techniques. Chromosomal DNA was prepared by standard techniques. Plasmid DNA was prepared by the alkaline lysis method of Ish-Horowicz & Burke (198 1). When preparing plasmid DNA from B. subtilis, the cells were treated with lysozyme ( 5 g 1-') prior to lysis with NaOH/SDS. Plasmid DNA from E. c ...
Lecture 2 Nucleic Acid Structure
Lecture 2 Nucleic Acid Structure

... -10 bp/turn -3.3 Å/bp -helix axis through center of bp -anti -C2' endo -wide major groove - binds peptides -narrow minor groove - binds planar molecules A form DNA -Right handed (more contact) -11 bp/turn -2.3 Å/bp (short/broader) ...
Ribosomal DNA sequences reveal gregarine pathogens
Ribosomal DNA sequences reveal gregarine pathogens

Chapter 6A
Chapter 6A

... length. Satellite DNA consists of 14-500 bp sequence units that tandemly repeat over 20-100 kb lengths of genomic DNA. Minisatellite DNA consists of 15-100 bp sequence units that tandemly repeat over 1-5 kb stretches of DNA. Microsatellite DNA consists of 113 bp units that can repeat up to 150 times ...
You Light Up My Life
You Light Up My Life

... • Some genes on chromosomes control cell growth and division • If something affects chromosome structure at or near these loci, cell division may spiral out of control ...
Genomic island analysis: Improved web-based software
Genomic island analysis: Improved web-based software

... Properties of genes in GIs? Defined a “putative island” as  8 or more genes in a row with dinucleotide bias  8 or more genes in a row with dinucleotide bias + an associated mobility gene Any difference for genes in islands versus outside of islands in terms of their protein Functional categories? ...
Mutations, Karyotyping, Pedigrees
Mutations, Karyotyping, Pedigrees

... Having any number of chromosomes that does not equal 23 pairs. Ex) Down Syndrome – 3 Chromosome 21’s ...
PPT
PPT

... Males only have a single X chromosome and will have recessive disorder on X chromosome automatically (do not have another X with a possible normal gene to counteract) ...
ch11_lecture
ch11_lecture

... • Some genes on chromosomes control cell growth and division • If something affects chromosome structure at or near these loci, cell division may spiral out of control ...
The Transcription Process
The Transcription Process

... The genetic code is frequently referred to as a "blueprint" because it contains the instructions a cell requires in order to sustain itself. We now know that there is more to these instructions than simply the sequence of letters in the nucleotide code, however. For example, vast amounts of evidence ...
Genetic Manipulation of Kinetoplastida
Genetic Manipulation of Kinetoplastida

... 2% or less) express the product of interest7, it is almost impossible to study the localization of introduced gene products by electron microscopy, and difficult to obtain sufficient RNA product for quantitation or structural analysis. Therefore, the creation of permanently transformed cell lines ha ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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