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Cloning in Escherichia coli
Cloning in Escherichia coli

... molecular biology. With just a small amount of sequence information about a target DNA molecule, a pair of short single-stranded primers complementary to the ends of this DNA molecule, and a special DNA polymerase enzyme, the PCR technique can amplify the sequence to produce enough DNA for most stan ...
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... BNIP3 can induce autophagy. However whether the consequence of the autophagy is the cell death or survival remains to be established. Since BNIP3 is induced by hypoxia through transcription factor HIF-1, it was postulated to play a role in hypoxia-induced cell death. Hypoxia-induced acidosis augment ...
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... Since the DNA strands are anti-parallel the template nucleotides have to be added in opposite directions. One strand moves in the same direction as the replication fork (leading strand) On the other template strand it moves in the opposite direction (lagging strand) DNA Ligase joins the fragments ...
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IUSTI Australia MAMEF poster

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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