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viral networks
viral networks

... • Protein/polypeptides can be from various sources; eukaryotes, prokaryotes, viruses and even artificial sequences…allows the comparison of interactomes w/in and between different species…in this paper, eukaryote (human) interactome vs. viral interactome ...
PDF
PDF

... magnetic or electric fields. This is then followed by their detection and analysis by appropriate software. In Proteomics, the two most common approaches used are: peptide mass fingerprinting and tandem mass MS sequencing. Additionally, liquid chromatography helps to separate the proteins before MS. ...
The amino acids
The amino acids

... Now that we know everything about the amino acids, we can look at the real goal of this whole excercise: sequence alignments. Which of the following alignments is better, left or right: ...


... 17. The protein myoglobin is found in numerous organisms, and the amino acid residue sequence of the protein from a wide variety of organisms has been determined. The word "conserved"is applied to a particular region of the protein if the sequence of that region is regular between several organisms ...
Biology and computers
Biology and computers

... PCR primers Analysis ...
What is the difference between RMSF? RMSD? B-Factor?
What is the difference between RMSF? RMSD? B-Factor?

... Tools for Analyzing Protein 3D Structures AMIGOS - Reads an RNA PDB file and outputs a complete table of torsion angle calculations. APBS - Software for evaluating the electrostatic properties of nanoscale biomolecular systems. Atlas of Protein Side-Chain Interactions - depicts how amino acid side- ...
Multiple Sequence Alignment
Multiple Sequence Alignment

... to deduce function PCR primers Analysis ...
CH 6: Proteins and Amino Acids
CH 6: Proteins and Amino Acids

... a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential aa (one exception, gelatin) – Plant sources are missing or low in at least one essential aa (one exception, soy) ...
Repetitive Patterns in Proteins
Repetitive Patterns in Proteins

... Problems: Only very few polypeptide sequences are capable of folding; protein folds are not very stable Repetition intrinsically promotes stability through the periodic recurrence of favorable interactions Modular reuse of already established components allows for a stepwise increase in complexity ( ...
cytology_chemicals
cytology_chemicals

...  Cellulose in human food passes undigested through the digestive tract as insoluble fiber  Some microbes use enzymes to digest cellulose  Many herbivores, from cows to termites, have symbiotic relationships with these microbes ...
No Slide Title
No Slide Title

... One can only transfer information if the similarity is significantly high between the two sequences. Schneider (group of Sander) determined the “threshold curve” for transferring structural information from one known protein structure to another protein sequence: If the sequences are > 80 aa long, t ...
Protein Structure Prediction and Display
Protein Structure Prediction and Display

... Uses table of propensities calculated primarily from structures determined by Xray crystallography  Table consists of one “likelihood” for each structure for each amino acid for each position in a 17 amino acid window ...
Slide 1
Slide 1

... of DNA, RNA and amino acid to identify the regions of similarity that may be a consequence of functional, structural or evolutionary relationship between the sequences. ...
Proteins are polymers consisting of amino acids linked by peptide
Proteins are polymers consisting of amino acids linked by peptide

... hydrophobic side chains from diverse parts of the protein chain- causes the protein chain to fold into compact, ordered form. Why this effect is thermodynamically favored? Entropy-driven process ...
Protein foods - Deans Community High School
Protein foods - Deans Community High School

...  the iodine test turns from brown to black if starch is in food.  the filter paper test goes transparent if fat is in food. Chemists also have a way to test whether foods contain protein. This test is called the soda lime test. Watch your teacher demonstrate (demo 3.40) what happens in the soda li ...
Proteins2[1]
Proteins2[1]

... – Dislphide bond (if any) ...
protpars
protpars

... One will usually want to use "?" after a stop codon, if one does not know what amino acid is there. If the DNA sequence has been observed there, one probably ought to resist putting in the amino acids that this DNA would ...
Slides
Slides

... and (due to the evolutionary connection) have similar function The sequence alignment problem is an optimization problem: produce the best alignment according to a scoring function A scoring function provide numeric values for each possible symbol pairing and for gaps in an alignment. ...
Protein engineering of aldolase: Directed evolution
Protein engineering of aldolase: Directed evolution

... Our directed evolution experiments as well as our mechanistic studies are focused on the FBP-aldolase and the related tagatose-1,6-bisphosphate aldolase, which cleaves a stereoisomer of FBP. Both enzymes belong to the (/)8-barrel family of protein fold. There is much interest in this class of fold ...
here
here

... BUT the most common sequences are simple repeats ...
exon f exon g
exon f exon g

... of proteins by assuming a protein chain consists of noninteracting atoms in a uniform sphere of radius equivalent to that of the corresponding protein. The normalized version (N-DOPE) was used instead of the raw score; it is a standard score (Z-score) derived from the statistics of raw DOPE scores. ...
Diversity in the Structure and Function of Amylase
Diversity in the Structure and Function of Amylase

... • What is the percent similarity in structure of amylase based on the phylogenetic tree? • How does the sequence identity of the sequences match the clustering in the phylogenetic tree? • Blast for percent sequence identity and percent sequence similarity. • This will help students to quantitatively ...
Phylogenetic analysis
Phylogenetic analysis

... the best available for carrying out data base similarity (homology searches). • 2) PAM (Dayhoff). These have been extremely widely used since the late '70s. • 3) GONNET. These matrices were derived using almost the same procedure as the Dayhoff one (above) but are much more up to date and are based ...
exam I answers
exam I answers

... below and the attached graph paper for your answers. Prostaglandins are a class of fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. Prostaglandins are responsible for producing fever and inflammation and its associated pain. They are derived from the 20-carbon ...
Protein Secondary Structure Prediction
Protein Secondary Structure Prediction

... the coordinates of residues. Tertiary Structure is the native state, or folded form, of a single protein chain. This form is also called the functional form. Tertiary structure of a protein includes the coordinates of its residues in three dimensional space. Quaternary Structure is the structure of ...
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Ancestral sequence reconstruction

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