A Novel Role for Vitamin K1 in a Tyrosine Phosphorylation
... facilitate the binding of vitamin K-dependent proteins to membrane phospholipids in the presence of calcium (4, 5). The vitamin K1 level in the mammalian fetus is tightly regulated by a maternal/fetal placental gradient (6); the median vitamin K1 concentration in human cord plasma is 16 pg/ml as com ...
... facilitate the binding of vitamin K-dependent proteins to membrane phospholipids in the presence of calcium (4, 5). The vitamin K1 level in the mammalian fetus is tightly regulated by a maternal/fetal placental gradient (6); the median vitamin K1 concentration in human cord plasma is 16 pg/ml as com ...
Probing protein function by chemical modification
... Therefore, bioorthogonal chemistry has been successfully used in genome-wide protein profiling, including proteomic analysis of glycoproteins and glycosidases [51–53] and activity-based protein profiling (ABPP) for the identification of hydrolases, proteases, kinases, phosphotases, and glycosidases ...
... Therefore, bioorthogonal chemistry has been successfully used in genome-wide protein profiling, including proteomic analysis of glycoproteins and glycosidases [51–53] and activity-based protein profiling (ABPP) for the identification of hydrolases, proteases, kinases, phosphotases, and glycosidases ...
Abundant Expression of ras Proteins in Aplysia Neurons
... was observed in neurons; little or no fluorescence was detected in the connective tissue sheath that surrounds the neuronal components (Fig. 3 A). Little immunoreactivity was seen in buccal muscle exposed photographically for the same short period of time (Fig. 3 C). No immunofluorescence was detect ...
... was observed in neurons; little or no fluorescence was detected in the connective tissue sheath that surrounds the neuronal components (Fig. 3 A). Little immunoreactivity was seen in buccal muscle exposed photographically for the same short period of time (Fig. 3 C). No immunofluorescence was detect ...
Application Note: Using the NanoDrop One to Quantify Protein and
... their workflows. This information helps them make informed decisions before proceeding with downstream experiments. There are many protein quantification methods to choose from including gravimetric approaches, colorimetric assays, direct spectrophotometric UV measurements (such as A280), and amino ...
... their workflows. This information helps them make informed decisions before proceeding with downstream experiments. There are many protein quantification methods to choose from including gravimetric approaches, colorimetric assays, direct spectrophotometric UV measurements (such as A280), and amino ...
A mutation which disrupts the hydrophobic core of the signal peptide
... whether the mutant enzyme was membrane bound, we prepared microsomes from the transduced COS-7 cells. The BUGT activity was present in the microsomal fraction of the COS-7 cells transfected with wild type and 15-Arg/B-UGT (results not shown). This indicates that the mutant enzyme is present in its n ...
... whether the mutant enzyme was membrane bound, we prepared microsomes from the transduced COS-7 cells. The BUGT activity was present in the microsomal fraction of the COS-7 cells transfected with wild type and 15-Arg/B-UGT (results not shown). This indicates that the mutant enzyme is present in its n ...
Laboratory Exercise #7: Column Chromatography of GFP proteins
... The lab is designed so that you can isolate the GFP protein from your bacterial cells. The bacterial cells you are working with have been genetically modified – i.e. transformed – with a pGLO plasmid that contains a gene for the Green Fluorescent Protein (GFP) from the jellyfish Aequoria victoria. A ...
... The lab is designed so that you can isolate the GFP protein from your bacterial cells. The bacterial cells you are working with have been genetically modified – i.e. transformed – with a pGLO plasmid that contains a gene for the Green Fluorescent Protein (GFP) from the jellyfish Aequoria victoria. A ...
Key Residues Defining the ~.t-OpioidReceptor Binding Pocket: A
... domains of the opioid receptors are exchanged to examine receptor selectivity (Kong et al., 1994; Wang et al., 1994; Xue et al., 1994, 1995; Fukuda et al., 1995; Hjorth et al., 1995; Meng et al., 1995; Minami et al., 1995; Onogi et al., 1995). The second approach involves three-dimensional computer ...
... domains of the opioid receptors are exchanged to examine receptor selectivity (Kong et al., 1994; Wang et al., 1994; Xue et al., 1994, 1995; Fukuda et al., 1995; Hjorth et al., 1995; Meng et al., 1995; Minami et al., 1995; Onogi et al., 1995). The second approach involves three-dimensional computer ...
Linköping University Post Print Distinct parts of leukotriene C-4 synthase
... A. - B. LTC4S interacts with 5-LO: The largest binding of [35S] 5-LO was seen with GST-LTC4S (87-150) whereas no binding was observed with GST-LTC4S (114-150) suggesting that the second hydrophilic loop (aa 90-113) mediated LTC4S binding of 5-LO. C. LTC4S interacts with FLAP: The largest binding of ...
... A. - B. LTC4S interacts with 5-LO: The largest binding of [35S] 5-LO was seen with GST-LTC4S (87-150) whereas no binding was observed with GST-LTC4S (114-150) suggesting that the second hydrophilic loop (aa 90-113) mediated LTC4S binding of 5-LO. C. LTC4S interacts with FLAP: The largest binding of ...
Diapositive 1 - International QSAR Foundation
... ► Iron yield of HO• radical via the Haber-Weiss cycle In the presence of reductants (metabolites such as ascorbate, cysteine, gluthatione) ...
... ► Iron yield of HO• radical via the Haber-Weiss cycle In the presence of reductants (metabolites such as ascorbate, cysteine, gluthatione) ...
PHARMACOLOGY (and other important compounds) Hey, Here is a
... of phosphorylase (activates) breakdown of glycogen increase in free glucose - Also activates triacylglycerol lipase (phosphorylated = active) Erythropoietin- [EPO], produced by kidney peritubular cells - works using a JAK/STAT signaling mechanism - release stimulated by hypoxia inducible factor- ...
... of phosphorylase (activates) breakdown of glycogen increase in free glucose - Also activates triacylglycerol lipase (phosphorylated = active) Erythropoietin- [EPO], produced by kidney peritubular cells - works using a JAK/STAT signaling mechanism - release stimulated by hypoxia inducible factor- ...
Rampant Adaptive Evolution in Regions of Proteins with Unknown
... primary targets of directional selection (e.g. [5–7]). However, there are no genome-scale analyses addressing how population genetic processes may differ between functionally annotated regions of proteins versus those regions with no known function. Protein domains serve a diversity of specialized f ...
... primary targets of directional selection (e.g. [5–7]). However, there are no genome-scale analyses addressing how population genetic processes may differ between functionally annotated regions of proteins versus those regions with no known function. Protein domains serve a diversity of specialized f ...
Document
... An Introduction To Cells In our model cell, a _____________separates the cell contents, called the____________, from its surroundings. The cytoplasm can be subdivided into the ____________a liquid, and intracellular structures collectively known as______________. Organelles are structures suspended ...
... An Introduction To Cells In our model cell, a _____________separates the cell contents, called the____________, from its surroundings. The cytoplasm can be subdivided into the ____________a liquid, and intracellular structures collectively known as______________. Organelles are structures suspended ...
SHORT COMMUNICATION Why Are Both Ends of the Polypeptide
... We extracted 2177 protein subunits6 solved by X-ray crystallography from the Protein Data Bank (PDB) of October 27, 2001. The proteins have ⬍30% amino acid sequence homology and were determined at 3.0 Å resolution or better. Of these, 393 subunits with ⬍100 amino acids were eliminated. Another 282 s ...
... We extracted 2177 protein subunits6 solved by X-ray crystallography from the Protein Data Bank (PDB) of October 27, 2001. The proteins have ⬍30% amino acid sequence homology and were determined at 3.0 Å resolution or better. Of these, 393 subunits with ⬍100 amino acids were eliminated. Another 282 s ...
Polyunsaturated Fatty Acids and Cardiovascular
... Cardiovascular disease (CVD) arises as a result of genetic predisposition in the context of a disease-promoting environment. While several risk factors have been identified for CVD, such as elevated serum lipid levels and hypertension, most of the genes identified thus far do not appear to involve s ...
... Cardiovascular disease (CVD) arises as a result of genetic predisposition in the context of a disease-promoting environment. While several risk factors have been identified for CVD, such as elevated serum lipid levels and hypertension, most of the genes identified thus far do not appear to involve s ...
Pentose Phosphate Shunt
... The complications of diabetes include a high propensity for cataract formation in later life Hyperglycemia is the cause, but why? Evidence points to the polyol pathway, in which glucose and other simple sugars are reduced in NADPH-dependent reactions Glucose is reduced by aldose reductase to sorbito ...
... The complications of diabetes include a high propensity for cataract formation in later life Hyperglycemia is the cause, but why? Evidence points to the polyol pathway, in which glucose and other simple sugars are reduced in NADPH-dependent reactions Glucose is reduced by aldose reductase to sorbito ...
Characterization of Ubiquitin/Proteasome
... The Hap2/3/4/5 complex is a heme-activated, CCAATT binding, global transcriptional activator of genes involved in respiration and mitochondrial biogenesis in the yeast species Saccharomyces cerevisiae. Hap4 is the regulatory subunit of the complex and its levels determine the activity of the complex ...
... The Hap2/3/4/5 complex is a heme-activated, CCAATT binding, global transcriptional activator of genes involved in respiration and mitochondrial biogenesis in the yeast species Saccharomyces cerevisiae. Hap4 is the regulatory subunit of the complex and its levels determine the activity of the complex ...
LAC OPERON: A CONCEPT TO BE CLEARED What is an
... In the absence of lactose, a repressor protein encoded by the regulatory gene binds to the lac operator and prevents its transcription. This repressor protein has 2 binding sites: 1. With the allolactose molecule, 2. With the operator site. Binding of allolactose (which is synthesized from the lacto ...
... In the absence of lactose, a repressor protein encoded by the regulatory gene binds to the lac operator and prevents its transcription. This repressor protein has 2 binding sites: 1. With the allolactose molecule, 2. With the operator site. Binding of allolactose (which is synthesized from the lacto ...
Patrick Cramer Anton Meinhart, Tobias Silberzahn and
... polymerase II (pol II)1 is coupled to mRNA processing, including 5⬘ capping and splicing and 3⬘-end formation of the transcript. This coupling is achieved by the binding of mRNA processing factors to the phosphorylated C-terminal repeat domain (CTD), a mobile extension of the catalytic core of pol I ...
... polymerase II (pol II)1 is coupled to mRNA processing, including 5⬘ capping and splicing and 3⬘-end formation of the transcript. This coupling is achieved by the binding of mRNA processing factors to the phosphorylated C-terminal repeat domain (CTD), a mobile extension of the catalytic core of pol I ...
tuesday_lect_prot_DBs
... automatically compare the nucleotide seq. to amino acid databases. These can search sequence similarities going from one reading frame to another. => Simple, You don’t have to worry about translating the sequence (see below) BLASTX and FastX are explained more in detail later ...
... automatically compare the nucleotide seq. to amino acid databases. These can search sequence similarities going from one reading frame to another. => Simple, You don’t have to worry about translating the sequence (see below) BLASTX and FastX are explained more in detail later ...
Protein_Informatics_Annotation
... orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)tartrate. [provided by RefSeq, Aug 2008]. ...
... orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)tartrate. [provided by RefSeq, Aug 2008]. ...
Transcript
... b. Proteins that carry out enzymatic activity or structural feature will stick together inside the cell in their normal state. (More the rule than exception.) c. Cell is not a bag of water with globular proteins floating, but little centers, places where things get done. This is dictated by protein- ...
... b. Proteins that carry out enzymatic activity or structural feature will stick together inside the cell in their normal state. (More the rule than exception.) c. Cell is not a bag of water with globular proteins floating, but little centers, places where things get done. This is dictated by protein- ...
Valea LifeScience09 R
... made up of pharmaceutically uninteresting parts, whereas only a small part of the protein is directly involved in its actual biological activity, for example forming the binding pocket of an enzyme or the epitope of an antigen. Those active parts of a protein often tend to be conserved between prote ...
... made up of pharmaceutically uninteresting parts, whereas only a small part of the protein is directly involved in its actual biological activity, for example forming the binding pocket of an enzyme or the epitope of an antigen. Those active parts of a protein often tend to be conserved between prote ...
Gene Section PKM2 (pyruvate kinase isoenzyme type M2) in Oncology and Haematology
... This leads to an expansion of all phosphometabolites above the pyruvate kinase reaction and an increased channeling of glucose carbons into synthetic processes, i.e. DNA, phospholipid and amino acid synthesis. Tumor cells contain high levels of dimeric M2-PK, which has therefore been termed 'Tumor M ...
... This leads to an expansion of all phosphometabolites above the pyruvate kinase reaction and an increased channeling of glucose carbons into synthetic processes, i.e. DNA, phospholipid and amino acid synthesis. Tumor cells contain high levels of dimeric M2-PK, which has therefore been termed 'Tumor M ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.