Leukaemia Section t(9;22)(q34;q11) in ALL Atlas of Genetics and Cytogenetics
... major breakpoint cluster region), a cluster of 5.8 kb, between exons 12 and 16, also called b1 to b5 of Mbcr; most breakpoints being either between b2 and b3, or between b3 and b4; transcript is 8.5 kb long; this results in a 210 kDa chimeric protein (P210), with the first 902 or 927 amino acids fro ...
... major breakpoint cluster region), a cluster of 5.8 kb, between exons 12 and 16, also called b1 to b5 of Mbcr; most breakpoints being either between b2 and b3, or between b3 and b4; transcript is 8.5 kb long; this results in a 210 kDa chimeric protein (P210), with the first 902 or 927 amino acids fro ...
Product Specification
... Recombinant full-length human BLK containing N-terminal GST tag expressed by baculovirus in Sf 9 insect cells. The gene accession number is BC007371. This material is sold for research purposes only. Specific Activity 579 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30 ...
... Recombinant full-length human BLK containing N-terminal GST tag expressed by baculovirus in Sf 9 insect cells. The gene accession number is BC007371. This material is sold for research purposes only. Specific Activity 579 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30 ...
Is β-pleated sheet the molecular conformation which dictates
... protein, for three types of secondary structure: α-helix, β-sheet and β-turns. The scheme requires uniform input for the prediction programs, produced by any word processor, spreadsheet, editor or database program and produces uniform output on a printer, a graphics screen or a file. The scheme is i ...
... protein, for three types of secondary structure: α-helix, β-sheet and β-turns. The scheme requires uniform input for the prediction programs, produced by any word processor, spreadsheet, editor or database program and produces uniform output on a printer, a graphics screen or a file. The scheme is i ...
Simple Models of Protein Folding
... A final major consideration involves the use of a more complicated interaction potential. The most simple two dimensional lattice model only involves interactions amongst nonsequential hydrophobic residues, which neglects favorable interactions amongst hydrophilic residues and solvent and unfavorabl ...
... A final major consideration involves the use of a more complicated interaction potential. The most simple two dimensional lattice model only involves interactions amongst nonsequential hydrophobic residues, which neglects favorable interactions amongst hydrophilic residues and solvent and unfavorabl ...
ADP-ribosyltransferases: plastic tools for inactivating protein and
... 7. Residues governing ADPRT target specificity The conserved topology of the active site crevice indicates that modern ADPRTs diverged from a primordial ADPRT, yielding a family of enzymes with a conserved NAD-binding fold but diverse target specificities for ADP-ribosylation (Bazan and Koch-Nolte, ...
... 7. Residues governing ADPRT target specificity The conserved topology of the active site crevice indicates that modern ADPRTs diverged from a primordial ADPRT, yielding a family of enzymes with a conserved NAD-binding fold but diverse target specificities for ADP-ribosylation (Bazan and Koch-Nolte, ...
Proteins in Serum and Urine - Ústav lékařské biochemie a
... inflammation, tissue injury due to trauma or surgery, or tumor proliferation. In many other situations the reaction is present in a less pronounced form, such as the condition following an extreme physical exercise, acute heart stroke or around delivery. Simply stated, the acute phase reaction is tr ...
... inflammation, tissue injury due to trauma or surgery, or tumor proliferation. In many other situations the reaction is present in a less pronounced form, such as the condition following an extreme physical exercise, acute heart stroke or around delivery. Simply stated, the acute phase reaction is tr ...
ProteinPilot Descriptive Statistics Template
... in the data (Figure 3). Analysis as a function of retention time allows assessment of the time stability of an instrument’s calibration, while analysis as a function of peptide intensity gives a description of mass accuracy that allows comparison even across samples with different complexity. ...
... in the data (Figure 3). Analysis as a function of retention time allows assessment of the time stability of an instrument’s calibration, while analysis as a function of peptide intensity gives a description of mass accuracy that allows comparison even across samples with different complexity. ...
SOD binds cell-adhesive peroxidase - Journal of Cell Science
... surface; it could be released from the membrane with high salt. It was thus concluded that the peroxinectin-binding protein is an extracellular SOD (EC-SOD) and a peripheral membrane protein, presumably kept at the cell surface via ionic interaction with its C-terminal region. This interaction with ...
... surface; it could be released from the membrane with high salt. It was thus concluded that the peroxinectin-binding protein is an extracellular SOD (EC-SOD) and a peripheral membrane protein, presumably kept at the cell surface via ionic interaction with its C-terminal region. This interaction with ...
Sequence to Structure Analysis of DOPA Protein from Mucuna
... soil due to its ability to fix nitrogen [10]. In our study we have considered M. pruriens, which is considered to be one of the most popular Indian medicinal plant, traditionaly used in Ayurvedic Indian medicine, for treatment of different diseases including parkinsonism, male infertility, nervous d ...
... soil due to its ability to fix nitrogen [10]. In our study we have considered M. pruriens, which is considered to be one of the most popular Indian medicinal plant, traditionaly used in Ayurvedic Indian medicine, for treatment of different diseases including parkinsonism, male infertility, nervous d ...
How to study proteins by circular dichroism
... conditions where particular secondary structures are predominant (Section 4.2) cannot be analysed reliably at present. In Section 10, we will describe how an assessment of the reliability of the various methods of structural analysis of proteins can be made. For conventional CD instruments with a Xe ...
... conditions where particular secondary structures are predominant (Section 4.2) cannot be analysed reliably at present. In Section 10, we will describe how an assessment of the reliability of the various methods of structural analysis of proteins can be made. For conventional CD instruments with a Xe ...
How to study proteins by circular dichroism
... conditions where particular secondary structures are predominant (Section 4.2) cannot be analysed reliably at present. In Section 10, we will describe how an assessment of the reliability of the various methods of structural analysis of proteins can be made. For conventional CD instruments with a Xe ...
... conditions where particular secondary structures are predominant (Section 4.2) cannot be analysed reliably at present. In Section 10, we will describe how an assessment of the reliability of the various methods of structural analysis of proteins can be made. For conventional CD instruments with a Xe ...
... free water. The real-time PCR program was 95 °C for 5s, followed by 45cycles of 95 °C for 5s, 60 °C for 34s. Dissociation melting curves analysis of amplification products was performed at the end of each PCR reaction to confirm that only one PCR product was amplified and detected. The relative quan ...
hal.archives-ouvertes.fr
... the involvement of such receptors in the sorting of storage proteins was discarded. The diversity of species and tissues used in those studies made difficult the interpretation of the results obtained so far. Recently, a tagged mutant for the AtVSR1 gene, a close homologue to pumpkin PV72, was shown ...
... the involvement of such receptors in the sorting of storage proteins was discarded. The diversity of species and tissues used in those studies made difficult the interpretation of the results obtained so far. Recently, a tagged mutant for the AtVSR1 gene, a close homologue to pumpkin PV72, was shown ...
TAS-9011-2006.pdf
... structure of all newly expressed proteins with all known allergens should be done. Searches should be conducted using various algorithms such as FASTA or BLASTP to predict overall structural similarities. Strategies such as stepwise contiguous identical amino acid segment searches may also be perfor ...
... structure of all newly expressed proteins with all known allergens should be done. Searches should be conducted using various algorithms such as FASTA or BLASTP to predict overall structural similarities. Strategies such as stepwise contiguous identical amino acid segment searches may also be perfor ...
a pdf of this article as it appeared in Projects in Scientific
... large systems, of which the nuclear pore is one example. “The XT3,” says Schulten, “is a terrific computer platform for this new generation of work.” Encouraged by success with the NPC, Schulten has (as of late August 2006) begun to use his powerful software NAMD on PSC’s XT3 to simulate the riboso ...
... large systems, of which the nuclear pore is one example. “The XT3,” says Schulten, “is a terrific computer platform for this new generation of work.” Encouraged by success with the NPC, Schulten has (as of late August 2006) begun to use his powerful software NAMD on PSC’s XT3 to simulate the riboso ...
as a PDF
... resonances of Dd 27ox Fd to the b-CH2 and a-CH protons of the cysteines that coordinate the clusters. Resonances A, N and J are correlated. Considering cross peak intensities, the T1 values and the temperature dependence behavior, it is possible to assign both Hb and Ha from the same cysteinyl resid ...
... resonances of Dd 27ox Fd to the b-CH2 and a-CH protons of the cysteines that coordinate the clusters. Resonances A, N and J are correlated. Considering cross peak intensities, the T1 values and the temperature dependence behavior, it is possible to assign both Hb and Ha from the same cysteinyl resid ...
Chem 301 Biological Chemistry I Laboratory Lab 7: Protein
... moments that, when exposed to an external magnetic field, will align either with (lower energy) or against (higher energy) the applied field. These two energy states are the energy states that are “probed” by NMR. Nuclei that are “spin-1/2 nuclei” have NMR signals; these include 1H, 13C, 15N, 19F, a ...
... moments that, when exposed to an external magnetic field, will align either with (lower energy) or against (higher energy) the applied field. These two energy states are the energy states that are “probed” by NMR. Nuclei that are “spin-1/2 nuclei” have NMR signals; these include 1H, 13C, 15N, 19F, a ...
Task - Illustrative Mathematics
... This task is a variant of 8.EE Quinoa Pasta 1, where all the relevant information is given as part of the task statements and the students are asked to set up a system of equations. Another variant of this task is given in A-REI.6 Quinoa Pasta 3, which is a more open-ended modeling task. It poses th ...
... This task is a variant of 8.EE Quinoa Pasta 1, where all the relevant information is given as part of the task statements and the students are asked to set up a system of equations. Another variant of this task is given in A-REI.6 Quinoa Pasta 3, which is a more open-ended modeling task. It poses th ...
The balancing act between the cellular processes of protein
... differences contribute to sarcopenia or can be reversed with resistance exercise training and/or leucine-enriched essential amino acid supplementation remains to be determined. The review by Rose and Richter (6) focuses on molecular signaling in muscle during (rather than after) exercise that contri ...
... differences contribute to sarcopenia or can be reversed with resistance exercise training and/or leucine-enriched essential amino acid supplementation remains to be determined. The review by Rose and Richter (6) focuses on molecular signaling in muscle during (rather than after) exercise that contri ...
Powerpoint
... Gaia - a complex entity involving the Earth's biosphere, atmosphere, oceans, and soil; the totality constituting a feedback system which seeks an optimal physical and chemical environment for life on this planet. James Lovelock ...
... Gaia - a complex entity involving the Earth's biosphere, atmosphere, oceans, and soil; the totality constituting a feedback system which seeks an optimal physical and chemical environment for life on this planet. James Lovelock ...
Amino Acid Metabolism
... Amino acids are the “building-blocks” of proteins. Protein, from the Greek word meaning “of prime importance,” constitute and array of structures. Examples of these structures include hormones, enzymes, and muscle tissue. The primary function of protein is growth and repair of body tissue (anabolism ...
... Amino acids are the “building-blocks” of proteins. Protein, from the Greek word meaning “of prime importance,” constitute and array of structures. Examples of these structures include hormones, enzymes, and muscle tissue. The primary function of protein is growth and repair of body tissue (anabolism ...
Pvlea-18, a Member of a New Late-Embryogenesis
... F. Campos, A. Garciarrubio, A.A. Covarrubias [1997] Plant Mol Biol 35: 393–405). We show that the Pvlea-18 gene encodes a 14-kD protein that accumulates during late embryogenesis. Related proteins have been detected in both monocots and dicots, indicating that PvLEA-18 is a member of a new family of ...
... F. Campos, A. Garciarrubio, A.A. Covarrubias [1997] Plant Mol Biol 35: 393–405). We show that the Pvlea-18 gene encodes a 14-kD protein that accumulates during late embryogenesis. Related proteins have been detected in both monocots and dicots, indicating that PvLEA-18 is a member of a new family of ...
Site-specific functionalization of proteins and their applications to
... reduced number of lysines and the lack of an Fc region. There have been attempts to make this modification more specific by using preferential N-terminal labeling [ ] or kinetically controlled lysine labeling [2]. Generally those methods suffer from low yields or require complex steps including the ...
... reduced number of lysines and the lack of an Fc region. There have been attempts to make this modification more specific by using preferential N-terminal labeling [ ] or kinetically controlled lysine labeling [2]. Generally those methods suffer from low yields or require complex steps including the ...
2/17/12 Gene regulation
... 8.2 DNA-Binding Proteins • Most DNA-binding proteins interact with DNA in a sequence-specific manner • Specificity provided by interactions between amino acid side chains and chemical groups on the bases and sugar–phosphate backbone of DNA • Major groove of DNA is the main site of protein binding • ...
... 8.2 DNA-Binding Proteins • Most DNA-binding proteins interact with DNA in a sequence-specific manner • Specificity provided by interactions between amino acid side chains and chemical groups on the bases and sugar–phosphate backbone of DNA • Major groove of DNA is the main site of protein binding • ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.