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L - Centre for Genomic Regulation
L - Centre for Genomic Regulation

... copies have descended side by side during the history of an organism, (for example, alpha and beta hemoglobin) the genes should be called paralogous (para = in parallel). Where the homology is the result of speciation so that the history of the gene reflects the history of the species (for example a ...
Gene Section STEAP2 (six transmembrane epithelial antigen of the prostate 2)
Gene Section STEAP2 (six transmembrane epithelial antigen of the prostate 2)

... STEAP2 is highly expressed in androgen receptorpositive prostate cancer cell line LNCaP, but not in the androgen receptor-negative prostate cancer cell lines PC-3 and DU-145. However, STEAP2 expression is not significantly regulated by androgens in prostate cancer cells (Korkmaz et al., 2002). In hu ...
Bio/CS 251 Bioinformatics
Bio/CS 251 Bioinformatics

... because this peptide is uniformly hydrophobic. This hydrophobic peptide could also be found in a lipid bilayer, e.g., the cell membrane. (b) Which peptide could form an amphipathic -sheet? Why? Where in the protein would this peptide most likely be found. Why? Peptide B could form an amphipathic - ...
Gene Section myeloid leukemia 1; aml1 oncogene)) Atlas of Genetics and Cytogenetics
Gene Section myeloid leukemia 1; aml1 oncogene)) Atlas of Genetics and Cytogenetics

... AML1 and partners - recurrent translocations. Editor 02/2003; updated 08/2003. Note: cases of AML1 translocations with either 4q21, 4q27, 8q24 (not the TRPS1 one), 11q24,14q11, 16p13 hav e been prov ed to be cry ptic t(12;21) with ETV6/AML1 inv olv ement, and we hav e theref ore to be cautious with ...


... whole structure of the fusion protein construction is made up of, Clostridium epsilon toxin ETX/ MTX2, family member which is combined with Leucocidin/ASH4 hemolysin domain. The nucleotide and derived amino acid sequences of the epsilon toxin gene are located between the start codon at the base 188 ...
SUPPORTING INFORMATION Manipulating fatty acid biosynthesis
SUPPORTING INFORMATION Manipulating fatty acid biosynthesis

... Protein sequences of Cr-cACP (ACP2, Q6UKY5_CHLRE), Cr-mACP (ACP1, Q6UKY4_CHLRE), CrTE (FAT1, A8HY17_CHLRE), ChTE (FATB2, Q39514_CUPHO) and UcTE (FATB1, Q41635) were obtained from the UniProt database. Sequence alignments were conducted using TCOFFEE [1] and ESPRIPT [2], based on the secondary struct ...
Manipulating Genes - whssbiozone
Manipulating Genes - whssbiozone

... In the matter of using this biotechnology to further the studies in how it can help treat or prevent diseases that may have no other cure, this should be studied further. Doctors may be able to treat the disorders by inserting a gene into the patient's cells instead of using drugs or surgery. Resear ...
Manuals: XL1-Red Competent Cells
Manuals: XL1-Red Competent Cells

... plasmid with a lower copy number than the pBluescript® phagemid is being used, the cells can be diluted and grown overnight for as many cycles as desired. 14. Prepare miniprep DNA from 1.5 ml of the overnight culture in order to isolate the randomly mutated plasmid DNA. Stratagene's StrataPrep® plas ...
PROTEIN SUBCELLULAR LOCALIZATION - PolyU
PROTEIN SUBCELLULAR LOCALIZATION - PolyU

... location. This kind of methods can achieve a very high accuracy as long as the homologs of the query sequences can be found in protein databases [8]. Over the years, a number of homology-based predictors have been proposed. For example, Proteome Analyst [9] computes the feature vectors for classific ...
Midwest climate summary - US Soybean Export Council
Midwest climate summary - US Soybean Export Council

... As is noted in most years, Western Corn Belt states showed lower protein concentrations than the US crop as a whole. Soybeans grown in the Southeast and East Coast states tended to have higher protein concentrations. Midsouth, Southeastern, and East Coast states produced a soybean crop with higher o ...
NH 2
NH 2

... - living organisms employ enzymes to form peptide bonds. eg. during translation process. - When two amino acids are linked together, the product is called a dipeptide and when the product is of three amino acids then it is tripeptide ...
Case
Case

... are generally only interested in SNPs If you are a cancer geneticist looking at sequence data from tumors, you are primarily interested in SNVs Outside humans, there are other complications (e.g. polyploidy, HGT etc.). Definitions vary by field ...
Thin-Layer Chromatography of Amino Acids
Thin-Layer Chromatography of Amino Acids

... the substance moved 5 mm, the solvent moved 10 mm. It also means if the substance moved 50 mm, the solvent moved 100 mm. ...
Degradation signals within both terminal domains of the cauliflower
Degradation signals within both terminal domains of the cauliflower

... (a) The UPR technique. Reference and test proteins are separated by a ubiquitin moiety. This fusion is cleaved by ubiquitin (Ub)-speci®c proteases at the Ub±test protein junction, yielding equimolar amounts of both proteins (Levy et al., 1996). For this study the system was adapted by using two stab ...
Genomics Bioinformatics Medicine. Institute of Medicine, October 15, 2002, Washington DC
Genomics Bioinformatics Medicine. Institute of Medicine, October 15, 2002, Washington DC

... genome loci ...
Slid 7 Hops
Slid 7 Hops

... 5- Further desaturation of the crepenynic acid by using Delta 14 desaturase enzyme (form double bond at position 14) leads to the formation of dehydrocrepenynic acid which has two double bonds one at position 9 and the other at position 14, is only found in fungi. 6- Then delta 14 Acetylenase form t ...
Anabaena - Oxford Academic
Anabaena - Oxford Academic

... chococcus sp. PCC 7942 exhibited similar dark respiratory activity, as measured by oxygen uptake, to that of the wild-type [21]. Thus cyanobacteria may employ an alternative respiratory pathway when the OPP is non-functional. The OPP is also thought to be largely responsible for the supply of reduct ...
lactase persistence: evidence for selection
lactase persistence: evidence for selection

... There was strong selection pressure for the lactase-persistence allele. This selection caused the allele (and surrounding DNA) to be passed on from one generation to the next. Evidence for this selective sweep can be seen as a large area of homozygosity in all lactase-persistent individuals. Because ...
Nature of Sex Chromosomes
Nature of Sex Chromosomes

... usually remains unchanged from generation to generation but changes do sometimes occur and the number of repeats may increase or decrease. ...
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection

... asparagine at position 11, the N-terminally shortened peptides both without glutamic acid of position 1 and glutamine of position 2 lead to a binding signal comparable to that of the untruncated peptide. A drastic effect amounting to the complete loss of detectable binding signal is associated with ...
Katsanis - Noble Research Lab
Katsanis - Noble Research Lab

... Group for the selection and ranking of the markers is unclear, and the literature offers little information relevant to whether (and the extent to which) any of these markers are causally related to phenotypes (1,2). Moreover, a quick review of the literature of linkage analyses and genome wide asso ...
Measuring Arsenite using E. coli reporter strains
Measuring Arsenite using E. coli reporter strains

... 4197 (Gfp intensity, fluorescence assay) Principle Resistance to arsenite and arsenate is a natural phenomenon, which is present in many different bacteria. The resistance mechanism functions by pumping any entering arsenite out from the cell by use of a specific membrane protein complex. Arsenate w ...
Folds
Folds

... each class contains many different folds further classified into families no necessary functional connection is in this type of classification: a certain type of function is often, but not always, restricted to a certain type of fold (convergent evolution) – fold of a protein is “only” a scaffold to ...
- Circle of Docs
- Circle of Docs

... heat is needed for the reaction to occur E. substitution 88. What is the total number of high energy phosphates generated by 1 mole of acetyl Co-A through one round of the Tricarboxycylic cycle A. B. C. D. E. ...
Bioinfo_Course_Rotterdam
Bioinfo_Course_Rotterdam

... Finding back precise information A bit of history will explain some terms and avoid further confusion. In the 1980s, several databases started to collect sequence information: GenBank in the USA, EMBL in Europe and DDBJ in Japan for DNA; SwissProt in Switzerland and PIR in the USA for proteins; PDB ...
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Point mutation



A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.
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